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Genomic Tools to Accelerate Improvement in Okra (Abelmoschus esculentus) 基因组工具加速秋葵(Abelmoschus esculentus)改良
Pub Date : 2021-05-20 DOI: 10.5772/INTECHOPEN.97005
S. Lata, R. Yadav, B. S. Tomar
Okra (Abelmoschus esculentus L. Moench), is an important vegetable crop with limited studies on genomics. It is considered as an essential constituent for balanced food due to its dietary fibers, amino-acid and vitamins. It is most widely cultivated for its pods throughout Asia and Africa. Most of the okra cultivation is done exclusively in the developing countries of Asia and Africa with very poor productivity. India ranks first in the world with a production of 6.3 million MT (72% of the total world production). Cultivated okra is mostly susceptible to a large number of begomoviruses. Yellow vein mosaic disease (YVMD) caused by Yellow vein mosaic virus (YVMV) of genus Begomovirus (family Geminiviridae) results in the serious losses in okra cultivation. Symptoms of YVMD are chlorosis and yellowing of veins and veinlets at various levels, small size leaves, lesser and smaller fruits, and stunting growth. The loss in yield, due to YVMD in okra was found ranging from 30 to 100% depending on the age of the plant at the time of infection. Exploitation of biotechnological tools in okra improvement programmes is often restricted, due to the non availability of abundant polymorphic molecular markers and defined genetic maps. Moreover, okra genome is allopolyploid in nature and possess a large number of chromosomes (2n = 56–196) which makes it more complicated. Genomics tools like RNA- seq. for transcriptome analysis has emerged as a powerful tool to identify novel transcript/gene sequences in non-model plants like okra.
秋葵(Abelmoschus esculentus L. Moench)是一种重要的蔬菜作物,基因组学研究较少。由于富含膳食纤维、氨基酸和维生素,它被认为是均衡食物的必要成分。它的豆荚在亚洲和非洲被广泛种植。大部分秋葵种植都是在亚洲和非洲的发展中国家进行的,这些国家的生产力非常低。印度以630万吨的产量排名世界第一(占世界总产量的72%)。栽培的秋葵最容易感染大量的begomovirus。黄脉花叶病(YVMD)是由双病毒科begomavirus属的黄脉花叶病毒(YVMV)引起的黄秋葵病,是造成黄秋葵种植严重损失的主要原因。YVMD的症状是不同程度的静脉和小静脉变绿和变黄,叶片小,果实越来越小,生长发育迟缓。秋葵YVMD造成的产量损失在30%到100%之间,这取决于感染时植株的年龄。由于缺乏丰富的多态分子标记和确定的遗传图谱,在秋葵改良计划中利用生物技术工具往往受到限制。此外,秋葵基因组本质上是异源多倍体,并且拥有大量的染色体(2n = 56-196),这使得它的基因组结构更加复杂。基因组学工具如RNA- seq。转录组分析已成为鉴定秋葵等非模式植物中新的转录/基因序列的有力工具。
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引用次数: 2
Wild Progenitor and Landraces Led Genetic Gain in the Modern-Day Maize (Zea maysL.) 野生祖先和地方品种导致现代玉米的遗传增益(玉米)
Pub Date : 2021-03-20 DOI: 10.5772/INTECHOPEN.96865
D. Sharma, Rajesh K. Khulbe, Ramesh S. Pal, Jeevan Bettanaika, L. Kant
Maize (Zea mays ssp. mays) originated from Mexico and Central America and grew worldwide for food, feed and industrial products components. It possesses ten chromosomes with a genome size of 2.3 gigabases. Teosinte (Z. mays ssp. parviglumis) is the probable progenitor of the modern-day maize. The maize domestication favored standing gain of function and regulatory variations acquired the convergent phenotypes. The genomic loci teosinte branched 1 (tb1) and teosinte glume architecture 1 (tga1) played a central role in transforming teosinte to modern-day maize. Under domestication and crop improvement, only 2% (~1200) genes were undergone selection, out of ~60000 genes. Around ~98% of the genes have not experienced selection; there is enormous variation present in the diverse inbred lines that can be potentially utilized to identify QTLs and crop improvement through plant breeding. The genomic resources of wild relatives and landraces harbor the unexplored genes/alleles for biotic/abiotic tolerance, productivity and nutritional quality. The human-made evolution led to the transformation of wild relatives/landraces to the modern-day maize. This chapter summarized the maize’s wild relatives/landraces and the genetic gain over time in biotic/abiotic, productivity, and nutritional quality traits.
玉米(玉米是甜的)。mays)起源于墨西哥和中美洲,并在世界范围内种植,用于食品,饲料和工业产品组件。它拥有10条染色体,基因组大小为2.3千兆酶。大刍草;大刍草;Parviglumis)很可能是现代玉米的祖先。玉米驯化有利于直立性功能增益和调控变异获得趋同表型。基因组位点teosinte branched 1 (tb1)和teosinte glume architecture 1 (tga1)在teosinte向现代玉米的转变中发挥了核心作用。在驯化和作物改良过程中,在约60000个基因中,只有2%(约1200个)基因发生了选择。大约98%的基因没有经历选择;在不同的自交系中存在着巨大的变异,这些变异可以潜在地用于识别qtl和通过植物育种进行作物改良。野生近缘种和地方品种的基因组资源蕴藏着生物/非生物耐受性、生产力和营养品质方面尚未开发的基因/等位基因。人类的进化导致了野生近缘种/地方品种向现代玉米的转变。本章总结了玉米的野生近缘种/地方品种及其在生物/非生物、生产力和营养品质性状方面的遗传增益。
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引用次数: 2
Reality of Mitogenome Investigation in Preservation of Native Domestic Sheep Breeds 本土家羊品种有丝分裂基因组保存研究现状
Pub Date : 2021-03-03 DOI: 10.5772/INTECHOPEN.95768
A. Gáspárdy
This chapter deals with the study of extranuclear hereditary material and the possibilities of using it to maintain endangered animal breeds. The chapter characterizes mtDNA, presents its genes and their functions, while also emphasizing the hypervariable control region. It reports on the results of previous researches, referring to international publications. It sheds light on promising areas of mitogenomic research. It shows the maternal genetic background of local native varieties according to the results of the study of available country/geographical region. It deals with reasons for endangerment and the arguments for preservation of autochthonous breeds. In addition, it gives place to discuss some exciting professional concepts in rare breed preservation.
本章涉及核外遗传物质的研究和利用它来维持濒危动物品种的可能性。本章描述了mtDNA的特征,介绍了其基因及其功能,同时也强调了高变控制区。它报告了以前的研究结果,参考了国际出版物。它揭示了有丝分裂基因组研究的前景。根据现有国家/地理区域的研究结果,显示了本地品种的母系遗传背景。它讨论了濒危的原因和保护本土品种的论点。此外,本文还讨论了稀有品种保护中一些令人振奋的专业概念。
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引用次数: 0
On-Farm Crop Diversity for Advancing Food Security and Nutrition 促进粮食安全和营养的农田作物多样性
Pub Date : 2021-02-26 DOI: 10.5772/INTECHOPEN.96067
B. Furman, A. Noorani, C. Mba
In 2019, nearly 690 million people were hungry, indicating that the achievement of Zero Hunger by 2030 is not on-track. The enhanced conservation and use of crop diversity, which demonstrably improves farm productivity and hence food security and nutrition, could be one of the solutions to this problem. The broadening of the inter- and intra-specific diversity of crops contributes to dietary diversification and nutrition and improves the resilience of production systems to shocks, especially the biotic and abiotic stresses attributed to climate change. Examples of successful interventions that resulted in enhanced on-farm crop diversity are provided. Relevant tools and guidelines to strengthen national capacities for the enhanced on-farm management of plant genetic resources for food and agriculture are also highlighted. Guidance, based primarily on the Second Global Plan of Action for Plant Genetic Resources for Food and Agriculture, is presented to enable the conservation of farmers’ varieties/landraces, their genetic improvement and seed delivery systems; promote their cultivation, consumption and marketing; develop and implement policies; foster partnerships and strengthen requisite institutional and human capacities. Finally, the case is made for research and development, including using modern techniques, to achieve these aims.
2019年,近6.9亿人处于饥饿状态,表明到2030年实现“零饥饿”的目标尚未步入正轨。加强作物多样性的保护和利用可以明显提高农业生产力,从而提高粮食安全和营养,这可能是解决这一问题的办法之一。作物种间和种内多样性的扩大有助于饮食多样化和营养,并提高生产系统抵御冲击的能力,特别是应对气候变化引起的生物和非生物压力的能力。还提供了一些成功干预措施的例子,这些措施提高了农田作物的多样性。报告还强调了加强国家能力以加强粮食和农业植物遗传资源农场管理的相关工具和准则。本指南主要基于《第二份粮食和农业植物遗传资源全球行动计划》,旨在促进农民品种/地方品种及其遗传改良和种子输送系统的保护;促进其种植、消费和销售;制定和实施政策;促进伙伴关系,加强必要的机构和人员能力。最后,提出了研究和开发的案例,包括使用现代技术,以实现这些目标。
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引用次数: 3
Vegetable Landraces: The “Gene Banks” for Traditional Farmers and Future Breeding Programs 蔬菜地方品种:传统农民和未来育种计划的“基因库”
Pub Date : 2021-02-17 DOI: 10.5772/INTECHOPEN.96138
R. Sumalan, S. Ciulca, R. Sumalan, S. Popescu
Crop diversity of vegetable species is threatened by the current homogenization of agricultural production systems due to specialization of plant breeders and increasing globalization in the seed sector. With the onset of modern agriculture, most traditional vegetable cultivars were replaced by highly productive and often genetically uniform commercial breeds and hybrids. This led to the loss of landraces, especially in countries with a super-intensive agriculture. The agricultural biodiversity erosion represents a huge risk for food safety and security. Vegetable landraces are associated with the cultural heritage of their place of origin being adapted to local agro-ecological areas and are more resilient to environmental stress than commercial cultivars. The chapter aim to highlight the importance of keeping and using vegetable landraces as valuable sources of genes for traditional farmers, but also for future breeding processes. We analyze the historical role of landraces, genetic diversity, high physiological adaptability to specific local conditions in association with traditional farming systems, as well as the breeding perspectives and evaluation of genetic diversity based on molecular markers.
由于植物育种专业化和种子部门日益全球化,目前农业生产系统的同质化威胁着蔬菜物种的作物多样性。随着现代农业的兴起,大多数传统的蔬菜品种被高产且通常基因一致的商业品种和杂交品种所取代。这导致了本土物种的消失,特别是在农业高度集约化的国家。农业生物多样性的侵蚀对食品安全构成了巨大的威胁。蔬菜地方品种与其原产地的文化遗产相关联,适应当地的农业生态区,比商业品种更能适应环境压力。本章旨在强调保存和利用蔬菜地方品种作为传统农民宝贵的基因来源的重要性,以及对未来育种过程的重要性。我们分析了地方品种的历史作用、遗传多样性、与传统耕作制度相关的对特定地方条件的高生理适应性,以及基于分子标记的育种前景和遗传多样性评价。
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引用次数: 1
Domestic Pig Germplasms of Andaman and Nicobar Islands 安达曼和尼科巴群岛家猪种质资源
Pub Date : 2021-02-11 DOI: 10.5772/INTECHOPEN.95916
Perumal Ponraj, A. De, D. Bhattacharya
Andaman and Nicobar Islands are endowed with immaculate flora and fauna biodiversity. Among the indigenous livestock species, pig occupies 27.26%. Andaman and Nicobar Islands have three different categories of domestic pig groups/breeds. Andaman Local pig is prevalent in Andaman group of Islands (South, Middle and North Andaman); Nicobari pig is in Nicobar group of Islands and long snouted Little Andaman wild pig (Schedule II animal under Forest Act, India). Other than the indigenous pigs, pure and crossbreds of Large White Yorkshire are available in Andaman and Nicobar Islands. Nicobari Pigs are reared exclusively by Nicobari tribes in Nicobar group of islands and create a well defined socio-economic-ecological status of their tribal society. Nicobari pig occupies a prominent place in custom, festivals and socio-economic status of Nicobari tribes. These Andaman local and Nicobari pigs are reared for meat purpose under free range or semi-intensive system. Nicobari pig is appeared as short, black/brownish in colour and living as a family. Andaman local pig is available in Andaman group of islands and body colour differs from rusty grey to black and brown. Neck and dorsal portion hair are long and thick whereas flank and sides hairs are shorter and thinner. Wild pig of Andaman (Sus scrofa andamanensis) is a most endangered porcine species of Andaman and Nicobar islands. Jarawa tribes in Andaman Islands prefer this wild pig as a good protein source. It is black in colour, short legged, small to medium sized and a prolific breeder. Litter size varies from 4 to 7 numbers. Another pig group is crossbred, cross between Large White Yorkshire and Andaman local or Nicobari pig. Crossbred pigs are light brown to complete white with different lines of blackish colour. This breed exhibits early maturity, high growth rate and fecundity. The Nicobari pig has high prolificacy as litter size is ranging from 8 to 10 numbers with good mothering ability and body weight of matured pig differs from 115 to 130 kg. Moreover, this crossbred is adapted highly to the local tropical humid environmental conditions and also can adjust with locally available feed resources on the different agricultural produces. This is highly suitable for commercial production of pork in this Andaman and Nicobar islands. However, the domestic pig breeds need to be protected and be conserved in this Andaman and Nicobar group of Islands.
安达曼和尼科巴群岛拥有完美的动植物生物多样性。在本地家畜种类中,猪占27.26%。安达曼和尼科巴群岛有三种不同的家猪群/品种。本地猪普遍存在于安达曼群岛(南、中、北安达曼);尼科巴猪是在尼科巴群岛和长鼻子小安达曼野猪(附表II动物根据森林法案,印度)。除了本地猪,大白约克郡的纯种和杂交品种也可在安达曼和尼科巴群岛。尼科巴利猪由尼科巴利群岛的尼科巴利部落专门饲养,并为其部落社会创造了明确的社会经济生态地位。尼科巴里猪在尼科巴里部落的习俗、节日和社会经济地位中占有突出地位。这些安达曼本地猪和尼科巴里猪是在自由放养或半集约化系统下饲养的。尼科巴里猪看起来很矮,黑色/棕色,像一个家庭一样生活。安达曼本地猪在安达曼群岛可以买到,身体颜色从锈灰色到黑色和棕色不等。颈部和背部的毛发长而厚,而侧面和侧面的毛发短而薄。安达曼野猪(Sus scrofa andamanensis)是安达曼和尼科巴群岛最濒危的猪种。安达曼群岛的加洛瓦部落更喜欢这种野猪,因为它是一种很好的蛋白质来源。它是黑色的,短腿,小到中等大小和一个多产的繁殖。产仔数从4到7只不等。另一种猪是杂交的,是大白约克郡猪和安达曼本地猪或尼科巴里猪的杂交。杂交猪的毛色从浅棕色到全白色,有不同的黑色线条。该品种早熟,生长速度快,繁殖力强。Nicobari猪产仔8 ~ 10头,产母能力强,成熟猪体重115 ~ 130公斤,具有较高的繁殖力。此外,该杂交品种对当地热带潮湿的环境条件适应性强,并能根据当地可获得的饲料资源对不同的农产品进行调整。这非常适合在安达曼和尼科巴群岛进行猪肉的商业化生产。然而,安达曼和尼科巴群岛的家猪品种需要得到保护和保存。
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Landraces - Traditional Variety and Natural Breed [Working Title]
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