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Contents of the microflora of the intestine, retention and productivity of broiler chickens in accordance with different types and quantities of the acidifier 不同酸化剂种类和用量对肉鸡肠道菌群含量、滞留率和产率的影响
Pub Date : 2018-12-27 DOI: 10.33245/2310-9289-2018-145-2-14-23
L. Dyachenko, E. Syvachenko, T. Syvyk
In two scientific-field experiments with broiler chicken cross-breeding ”Kobb-500", the goal was set to learn the influence of different dosages, forms and types of feeding the acidifier FRA LBB DRY onto the contents of the microflora of intestine, retention and productivity of broiler chickens.The first experiment was done using three groups-analogs of broiler chickens, 46 birds per group. The broiler chickens of the first group were the control group. This group was fed a regular mixed feed. The second and third groups were fed the same mixed feed with an addition of the acidifier of FRA LBB DRY feed added; 3kg per ton of feed for the 2-nd, and 5 kg per ton for the third group.The second experiment was done using 6 similar groups-analogs of broiler chickens, 100 birds per group, with an equal ratio of male to female chicken. Throw-out the experiment all six groups were fed the same amount of fully nutritional mixed feed, in accordance with their age growth groups. As far as drinking went, the groups were hydrated differently. Birds of the first group were getting plain water, broiler chickens of the 2, 3, 4, and 5 groups were getting a mix of FRA LBB DRY acidifier in the quantities of 1,0; 1,33; 1,66 and 2,0 ml/L accordingly. The last group, group 6, was getting a dose of liquid Norfolk antibiotic in the quantity of 1 ml/L.As the bacteriological experiments have shown, after 7 days of feeding different doses of dry acidifier, the stool sample of the second and third groups of broiler chickens, when compared to the first group, had shown to contain less undesirable microflora in particular Escherichia coli – by 26,7 and 29,0 %, enterococcus by 21,4 and 24,7 % with a high probability of difference (P<0,01). At the same time the test samples contained higher concentration of useful microflora such as Bifidobacterium and lacto bacteria. The difference, when compared to the- test sample, was 18,3–24,1 % (P<0,01) and 16,5-17,8 % (P<0,05).In the second experiment adding acidifier FRA LBB DRY to water for the 2, 3, 4, and 5-th groups by 1,0; 1,33; 1,66 and 2 ml/l of water had caused a decrease of escherichia coli by 12,7 % (P<0,05), 17,4 % (P<0,01), 18,8 % (P<0,01) and 16,0 % (P<0,05), enterococcus by 14,9; 20,9; 17,8; and 16,9 % with a high degree of probability (P<0,01), at the same time increasing the amount of bifidobacteria by 19,1; 20,6; 17,9 and 21,3 %, (P<0,01) and lactobacteria by 14,4; 16,5; 15,8; 12,4 % (P<0,01).The changes that had taken place in the contents of the microflora of intestine obviously had a positive effect on digestion and processing of nutrients, which, as a result, had improved the upkeep and productivity of the broiler chickens which were exposed to the acidifier. In the first experiment departure of the broiler chickens was almost three times less than that of a control group. In the second experiment the upkeep of broilers in test groups was larger than the control by 6–7 absolute percent.Adding an acidifier into t
本试验以杂交品种“Kobb-500”肉鸡为试验对象,研究了不同投喂酸化剂FRA LBB DRY的剂量、形式和类型对肉鸡肠道菌群含量、滞留量和生产能力的影响。第一个实验用三组肉仔鸡进行,每组46只鸡。第一组肉鸡为对照组。试验组饲喂常规混合饲料。第二组和第三组在相同的混合饲料中添加FRA - LBB - DRY饲料的酸化剂;第二组每吨饲料3公斤,第三组每吨饲料5公斤。第二个试验采用6个相似的肉鸡类似物组,每组100只鸡,雌雄比例相等。试验结束后,6个组均按年龄分组饲喂等量的全营养混合饲料。就饮酒而言,两组人的补水情况不同。第一组饲喂白开水,2、3、4、5组肉鸡饲喂FRA - LBB - DRY酸化剂,添加量分别为1、0;1、33;分别为1,66和2,0 ml/L。最后一组,即第六组,给予1 ml/L的诺福克液体抗生素。细菌学实验表明,在饲喂不同剂量的干酸化剂7天后,与第一组相比,第二组和第三组肉鸡的粪便样本中含有较少的不良微生物群,特别是大肠杆菌减少了26.7%和29.0%,肠球菌减少了21.4%和24.7%,差异很大(P< 0.01)。同时,检测样品中有益菌群如双歧杆菌和乳酸菌浓度较高。与未检测样本相比,差异为18.3 ~ 24.1% (P< 0.01)和16.5 ~ 17.8% (P< 0.05)。在第2、3、4、5组水中分别添加酸化剂FRA LBB DRY 1、0;1、33;1、66、2 ml/l水分别使大肠杆菌减少12.7% (P< 0.05)、17.4% (P< 0.01)、18.8% (P< 0.01)、16.0% (P< 0.05),肠球菌减少14.9%;20日,9;17、8;高概率增加16.9% (P< 0.01),同时双歧杆菌数量增加19.1%;20日,6;乳酸菌分别为17.9%和21.3% (P< 0.01)和14.4%;16、5;15日,8;12.4% (p < 0.01)。肠道菌群含量的变化对营养物质的消化和加工有明显的积极作用,从而提高了酸化剂处理肉鸡的维持力和生产能力。在第一次试验中,肉鸡的离群率几乎是对照组的三分之一。在第二个试验中,试验组肉鸡的饲养量比对照组高出6-7个百分点。在混合饲料中添加酸化剂也提高了鸟类的生长速度。例如,在第一次试验中,第2、3试验组的1只肉仔鸡的体质量比对照组增长了5.05、5.23 %。在第2次试验中,2 ~ 5组的绝对增重和日增重均比对照组高出3.8 ~ 4.5个百分点。就每增重1公斤的食用量而言,两项试验均表明,试验2 - 3组和试验2 - 5组的食用量比对照组少3,4和2,7 - 4,3 %。在显示实验效率的客观指标中,我们可以使用欧洲效率指数,在第一次和第二次实验中,该指数为37,8-38-6,32,0-50,比对照组高1分。除了酸化剂,该实验还将测试诺福克抗生素的使用效果,诺福克抗生素是在第二次实验中给第6组的鸟类服用的。数据表明,在绝对增重和日增重、饲料系数、畜禽滞留率和欧洲效率指数方面,第6组的鸡在所有类别上都明显超过对照组,但在几乎所有类别上都不及其他试验组。这些数据让我们得出结论,我们很有可能将抗生素换成酸化剂,而生产力几乎没有损失。综上所述,本微生物学研究的数据表明,在固定饲料中加入酸化剂对肉鸡肠道菌群有积极影响,这很可能是改善消化和消费过程的因素之一,从而改善了家禽的饲养。通过对试验结果的综合评价,肉鸡使用干液酸化剂FRA LBB dry的最佳投加量为混合饲料3kg/t或水1,33 - 1,66 ml/l。 从进一步发展的角度来看,这项研究可能会寻求一种不同的生化性质和更有效的酸化剂。关键词:肉鸡,酸化剂,抗生素,肠道菌群,产量
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引用次数: 0
Stabilization of enzyme prepareition protosubtilin G3X for use it on poultry farming 酶制剂原枯草菌素G3X用于家禽养殖的稳定性研究
Pub Date : 2018-12-27 DOI: 10.33245/2310-9289-2018-145-2-54-61
O. Selezniova, S. Tsehmistrenko, V. Polishchuk, S. Polishchuk
Enzyme preparations witchused in animal breeding are unstable. Instability is due to a partial or complete inactivation of the enzymes in the gastrointestinal tract under the influence of a strongly acidic environment, inhibitors and proteases. Increasing the effectiveness of the use of exogenous enzyme preparations is possible by the creation of stabilized forms of biopreparations. To do this, use the principles and methods of engineering enzymology. The aim of the research is to determine the optimal conditions for the immobilization of the enzyme preparation of protosubtilin G3X (proteolytic spectrum of the action) by the adsorption method. Conduct a comparative evaluation of the properties of native and immobilized biocatalysts on the conditions in vitro and in vivo.Proteolytic activity was determined by Anson's method. The amount of protein on the carrier was evaluated by reducing its concentration in the reaction mixture, measured with Lowry O.H. et al .. The activity of the immobilized enzyme was expressed as a percentage of the activity of the native enzyme. The pH solutions were measured on the potentiometer pH- 340. For immobilization weused enzyme preparation of protosubtilin G3X with an activity of 70 units / g, as a carrier we used zeolite.The immobilization procedure consisted of mixing the buffer solution of the enzyme with the carrier. During research and study of the influence of the ionic strength of the solution and pH on the adsorption process it was established that the catalytic activity of the obtained preparation falls in the buffers: phosphate, citrate, borate, acetate. Moreover, with an increase in the ionic strength of the solution, regardless of its composition, the enzyme activity of preparation was reduced. Optimal for immobilization was a 0.1 M phosphate buffer solution with pH in the range of 7.0–7.4. Protosubtilin G3X lost 80 % of the initial activity during immobilization in water.When determining the capacity of a carrier, it is found that 1 g of zeolite adsorbs 29.8 mg of protein. The maximum specific activity of the enzyme (0.16 U / mg protein) is appeared at the optimum load of the carrier 22.5 mg protein per 1 g zeolite and corresponded to 85.7 % of the activity of the native protosubtilin G3X. Consequently, the optimal conditions for the immobilization of protosubtilin G3X on zeolite are: 0.1 M phosphate buffer solution with pH 7.0–7.4, temperature 20–25 ºС, carrier capacity 22.5 mg / g, duration of the process 2 years.In experiments in vitro studied the dependence of the catalytic activity of the pH value in the range of 1.5 to 8.0. After 1 hour of incubation in the buffer solutions, it was found that the optimal value of pH for both forms of preparations coincides (pH 7.2). With pH-inactivation of native and immobilized forms of protosubtilin G3X, the loss of catalytic activity of the modified preparation was significantly less than the native. Moreover, a significant expansion of the pH profile in th
用于动物育种的酶制剂是不稳定的。不稳定性是由于胃肠道中的酶在强酸性环境、抑制剂和蛋白酶的影响下部分或完全失活。通过创建稳定形式的生物修复,可以增加外源性酶制剂使用的有效性。要做到这一点,使用工程酶学的原理和方法。本研究的目的是通过吸附法确定原枯草菌素G3X酶制剂的最佳固定化条件(作用蛋白水解谱)。在体外和体内条件下,对天然生物催化剂和固定化生物催化剂的性能进行比较评价。蛋白水解活性用Anson法测定。通过降低反应混合物中蛋白质的浓度来评估载体上的蛋白质量,由Lowry O.H.等人测量。固定化酶的活性以天然酶活性的百分比表示。pH溶液在电位计pH- 340上测定。固定化采用酶法制备活性为70单位/ g的原枯草菌素G3X,载体选用沸石。固定化过程包括将酶的缓冲溶液与载体混合。在研究溶液的离子强度和pH对吸附过程的影响时,确定了所获得的制剂的催化活性在缓冲液中下降:磷酸盐,柠檬酸盐,硼酸盐,醋酸盐。此外,随着溶液离子强度的增加,无论其组成如何,制备的酶活性都降低。固定的最佳条件是0.1 M的磷酸盐缓冲液,pH值在7.0-7.4之间。原枯草菌素G3X在水中固定期间失去了80%的初始活性。测定载体容量时,发现1g沸石可吸附29.8 mg蛋白质。在载体22.5 mg / 1 g沸石的最佳负载下,酶的最大比活性为0.16 U / mg蛋白,相当于原生枯草菌素G3X活性的85.7%。因此,原枯草菌素G3X在沸石上固定化的最佳条件为:0.1 M磷酸盐缓冲液,pH 7.0-7.4,温度20-25ºС,载体容量22.5 mg / g,工艺时间2年。在体外实验中研究了pH值在1.5 ~ 8.0范围内对催化活性的依赖性。在缓冲液中孵育1小时后,发现两种制剂的最佳pH值一致(pH值为7.2)。对原生和固定化形式的原枯草菌素G3X进行ph失活后,改性制剂的催化活性损失明显小于原生制剂。此外,固定化酶在酸性区域的pH分布显著扩大。如果天然酶在pH 5.0时保持20%的活性,在pH 4.5-4.8时不可逆失活;固定化酶在pH 4.0条件下保持42%的活性。研究了肉仔鸡胃肠道不同部位(甲状腺(pH 4.5 ~ 5.8)、腺胃(pH 3.6 ~ 4.7)、十二指肠(pH 5.7 ~ 6.2)消化酶的蛋白水解活性。原生和固定化原枯草菌素G3X组鸡甲状腺肿内容物的蛋白水解活性与对照组相同或高于对照组(p < 0.1),与固定化组相比提高了16.0% (p <0.05)。两试验组每增重1 kg的饲料成本相同(2.40 kg),比对照组低5.8%。关键词:酶,固定化,天然酶,沸石,吸附,蛋白水解活性,pH,缓冲溶液。
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引用次数: 0
Evaluanion of intensity of radionuclides increases in wheat production of body for growth succession 放射性核素强度在小麦生长发育过程中的影响
Pub Date : 2018-12-27 DOI: 10.33245/2310-9289-2018-145-2-72-77
G. Gutsol
The influence of liming of soils of honey fields with high pH on the intensity of the transition of cesium-137 and strontium-90 to protein production of beekeeping, in particular, flower pollen and pergo, was studied. This protein product contains about 250 substances and mineral elements: proteins and non-protein nitrogenous compounds – 20-25 %, sugars (sucrose, glucose, fructose, etc.) – 13-35 %, starch – 1-25 %, fiber – 3 -20 %, polovin – 6-20 %, crude fat – 2-14 %, ash – 1-6 %, water – 20-30 %. Pollen contains arytenoids, flavonoids, anthocyanins, higher alcohols, growth and other substances. In ashes of pollen, a lot of mineral elements, % of total ash: potassium 20-45, magnesium – 1-12, calcium – 1-15, silicon – 2-10, phosphorus – 1-20, iron – 0,1, sulfur – 1, manganese – 1.4. Other elements that affect the vital activity of the organism are also revealed. Beetroot obnizhya is used in the food industry, has antimicrobial effect, stimulates the immune system of the body, is used in cardiovascular diseases of the stomach and kidneys. The high efficiency of use of bee antlers in the treatment of diseases of the nervous system, lungs, liver, kidneys, glands of the internal secretion, vascular diseases of the brain, gastrointestinal tract, rheumatoid arthritis is established. Bee ankles increase appetite and overall body condition. Due to the high content of biologically active substances, it is widely used to increase the immunity of the body during the period of illness and surgical operations. Anesthetics also affect the process of hematopoietic, and increases hemoglobin and red blood cells. Agricultural honey plants are a powerful source of nectar and pollen, which are raw materials for the production of bee products. The main representatives are winter and spring rape, sunflower, buckwheat, turbot. Winter rice was grown in areas with soil pH of 4.7 with specific activity of cesium-137 – 31.0 and strontium-90 – 2.7 Bq / kg. Liming of soil under winter rape was carried out at the rate of 9 t / ha of brushwood along the surface of the soil with subsequent flooding. Limestone acidic soils with a pH of 4.7 reduced the acidity of the aqueous medium to 6.8. Reducing the acidity of the soil from pH 4.7 to 6.8 contributed to a decrease in the specific activity of cesium-137 in beer overgrowth by 40.0 %, in a perace – by 42.9 %, and by strontium-90, respectively, by 43.3 % and 42, 89 %. Specific activity of cesium-137 and strontium-90 in protein beekeepers did not exceed DR-2006. The coefficient of accumulation of cesium-137 and strontium-90 in beer overwork, produced from winter wheat rape, decreased by 31.8 % and 35.7 %, respectively – by 29.6 % and 31.6 % respectively. The greatest radiological efficacy was achieved with respect to cesium-137 in beer overwork, its accumulation decreased by 66.7 %, strontium-90 – in a proxy by 3.4 times.Comparing the intensity of the accumulation of radionuclides in protein production of beekeeping for liming soil
研究了高pH蜂蜜田土壤施石灰对铯-137和锶-90向养蜂生产蛋白质,特别是花粉和pergo的转化强度的影响。该蛋白质产品含有约250种物质和矿物元素:蛋白质和非蛋白质含氮化合物–20-25%,糖(蔗糖、葡萄糖、果糖等)–13-35%,淀粉–1-25%,纤维–3-20%,波洛温–6-20%,粗脂肪–2-14%,灰分–1-6%,水–20-30%。花粉中含有类芳基、黄酮类、花青素、高级醇、生长激素等物质。花粉灰中含有大量矿物元素,占总灰的%:钾20-45,镁1-12,钙1-15,硅2-10,磷1-20,铁-0.1,硫-1,锰-1.4。还揭示了影响生物体生命活动的其他元素。甜菜根用于食品工业,具有抗菌作用,刺激身体免疫系统,用于治疗胃和肾脏的心血管疾病。蜂角在治疗神经系统疾病、肺、肝、肾、内分泌腺、脑血管疾病、胃肠道疾病、类风湿性关节炎方面的高效应用已经确立。蜜蜂脚踝增加食欲和整体身体状况。由于生物活性物质含量高,它被广泛用于在疾病和外科手术期间提高身体免疫力。麻醉剂还会影响造血过程,增加血红蛋白和红细胞。农业蜂蜜植物是花蜜和花粉的强大来源,而花蜜和花粉是生产蜂产品的原材料。主要代表有冬春季油菜、向日葵、荞麦、大菱鲆。冬稻生长在土壤pH值为4.7的地区,比活性为铯-137–31.0和锶-90–2.7 Bq/kg。冬油菜以9 t/ha的灌木林速率在土壤表面进行石灰化,随后发生洪水。pH为4.7的石灰石酸性土壤将水介质的酸度降低到6.8。将土壤酸度从pH 4.7降低到6.8有助于啤酒过度生长中铯-137的比活性降低40.0%,在一段时间内降低42.9%,锶-90的比活性分别降低43.3%和42.89%。蛋白质养蜂人血清中铯-137和锶-90的比活均未超过DR-2006。冬小麦油菜生产的啤酒过劳中铯-137和锶-90的积累系数分别下降了31.8%和35.7%,分别下降了29.6%和31.6%。铯-137在啤酒过度工作中获得了最大的放射疗效,其积累减少了66.7%,锶-90减少了3.4倍。比较pH为4.7的石灰土养蜂生产蛋白质过程中放射性核素的积累强度,有必要注意到锶-90的还原效率高于铯-137。这种趋势是由于钙土的增加而观察到的,钙土的存在取决于锶-90对植物的同化强度。因此,蜜蜂虫洞中锶-90的还原强度低2.5倍;与铯-137相比,该配方是石灰土的14.4倍。从冬油菜中收获花粉的蜂科,在没有石灰的土壤上生长,产生的蛋白质产品数量较少。与土壤pH值为6.8的情况相比,从冬油菜中收获花粉用于石灰土壤的家庭产生的蜂艾增加了18.2%,perg增加了6.0%。与此同时,研究发现,与蜜蜂的虫洞相比,perogus中铯-137和锶-90的比活性分别高出2.8倍和7.0倍。关键词:蜂苹果,秘鲁,土壤,放射性核素,蜂蜜地,铯-137,锶-90。
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引用次数: 0
The effective use of Pietren breed at purebred and crossbred selection 在纯种和杂交选育中有效利用彼得伦品种
Pub Date : 2018-12-27 DOI: 10.33245/2310-9289-2018-145-2-38-45
O. Khmeliova, R. Stavetska
The article highlights the problem of finding optimal variants of breeding process in a herd of pigs at purebred and crossbred selection. For this purpose, the control and two experimental groups were formed: ♀ Pietrain × ♂ Pietrain (control), ♀ (Pietrain × Large White) × ♂ Large White (first experimental group), ♀ (Pietrain × Durok) × ♂ Pitren (second experimental group). The analysis of the animals performance in these groups was carried out in 40, 30 and 35 parities, respectively. The research was conducted on the basis of PE «Maglevovany M.I.» in the Donetsk region.It has been established that the higher level of reproductive characteristics have sows of first experimental group, which have heredity of Large White breed, this breed is traditionally considered as a maternal form in cross-breeding schemes.For instance, their litter size at birth is higher than the same indicator in the control and second experimental group (plus 0,5, P<0,05, and 0,3 piglets, respectively); by the litter size at weaning at 28 days (plus 1,6, P<0,001, and 0,6 piglets); by the litter size at 2 months (plus 1,6, P<0,001, and 0,4 piglets). The highest piglet survival at the age of 2 months was in the litters, whose ancestor were boars of Pietrain breed (second experimental group) − plus 3,6 and 2,3 %, respectively, compered to purebred piglets of Pietrain breed and the first experimental group.For such complex indexes as the litter weight at weaning and litter weight at 2 months the first experimental group had an advantage of 18,1 kg (P<0,001) and 31,1 kg (P<0,001) over the control group (purebred Pietrain) and 5,4 kg and 7,6 kg − over the second experimental group. However, the average piglet weight of sows of second experimental group both at 28 days and at 2 months are equal to the average piglet weight of the same age of the first experimental group.The piglets, which are descendants of boars of meat breeds Duroc and Pietrain show a tendency to the faster growth even in an early age. The avarage live weight of pigs of the second experimental group at the age of 100 days and 7 months is higher compared to avarage live weight of pigs of the same age of the control and the first experimental groups – plus 8,5–21,7 kg (P<0,01 for the second experimental group) and 4,4–13,5 kg (P<0,001 for the second experimental group), respectively. At the age of 100 days, the maximum average daily gain was obtained in the second experimental group with an advantage of 190 g (P<0,001) compared to control group and 115 g (P<0,001) compared to the first experimental group.It is obvious that hybrids with the faster growth, which were obtained through crossbreeding ♀ (Pietrain × Duroc) × ♂ Pietrain reach a live weight of 100 kg in a shorter period: they need less time needed to reach 100 kg of live weight – 32 days less (P<0,001 ) compared to purebred animals of Pietrain breed (control group) and 18 days less (P<0,001) compared to hybrids ♀ (Pietrain × Large White) × ♂ Large Whit
文章强调了在一群猪的纯种和杂交选择中寻找最佳育种过程变体的问题。为此,我们分为♀Pietrain ×♂Pietrain(对照组)、♀(Pietrain × Large White) ×♂Large White(第一实验组)、♀(Pietrain × Durok) ×♂Pitren(第二实验组)两组。分别以40胎、30胎和35胎对各组动物的生产性能进行分析。该研究是在顿涅茨克地区的“Maglevovany M.I.”的基础上进行的。研究表明,第一试验组母猪的生殖特征水平较高,该母猪遗传了大白品种,该品种在杂交方案中传统上被认为是母系品种。如初生窝产仔数均高于对照组和第二试验组(分别增加0,5头、P< 0.05和0,3头);28日龄断奶窝产仔数(外加1,6头(P< 0.001)和0,6头仔猪);2月龄窝产仔数(外加1,6头,P< 0.001, 0,4头)。2月龄时仔猪存活率最高的是Pietrain品种(第二试验组)的窝仔,分别比Pietrain品种的纯种仔猪和第一试验组提高了3.6%和2.3%。在断奶窝重和2月龄窝重等复杂指标上,试验组分别比对照组(纯种皮特兰)高18.1 kg (P< 0.001)和31.1 kg (P< 0.001),比试验组高5,4 kg和7,6 kg (P< 0.001)。而第二试验组母猪28日龄和2个月时的平均仔猪体重与第一试验组母猪同龄的平均仔猪体重相当。这些小猪是肉用品种杜洛克和皮特雷恩公猪的后代,在很小的时候就表现出更快的生长趋势。试验2组100日龄和7月龄猪的平均活重分别高于对照组和1组同龄猪的平均活重,分别增加8,5 - 21,7 kg (P< 0.01)和4,4 - 13,5 kg (P< 0.001)。100日龄时,第二试验组平均日增重最大,比对照组高190 g (P< 0.001),比第一试验组高115 g (P< 0.001)。通过杂交获得的雌(Pietrain ×杜洛克)×雄(Pietrain)杂种在较短的时间内达到100 kg的活重,达到100 kg活重所需的时间较雌(Pietrain ×大白)纯种(对照组)少32 d (P< 0.001),比雌(Pietrain ×大白)×雄(第一对照组)少18 d (P< 0.001)。在脂肪厚度等指标上,试验组杂交种以与对照组同龄(- 0.2 mm)的纯种猪占优势,说明肉用品种增肥能力较低。试验2组杂交猪的脂肪厚度比试验1组少0.9 mm (P< 0.001)。因此,在该母猪群中,为提高母猪的繁殖品质,建议采用♀(Pietrain × Large White) ×♂Large White杂交方案,并以♀(Pietrain × Duroc) ×♂Pietrain杂交方案为最佳方案。关键词:猪,皮特兰,大白猪,杜洛克,纯种和杂交选择,繁殖和肥育品质。
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引用次数: 0
Influence of feeding with live yeast on cow productivity and milk quality 饲喂活酵母对奶牛生产能力和牛奶品质的影响
Pub Date : 2018-12-27 DOI: 10.33245/2310-9289-2018-145-2-32-37
M. Panianchuk, O. Tytariova
A cow is, first and foremost, a ruminant animal. Its gastrointestinal tract is designed to digest a significant amount of structural fibers (fiber). Modern technological advances in genetics, breeding, feeding, retention and cows’ milking make it possible to obtain more than 10,000 kg of lactation milk from a cow, but this can be achieved only if a significant amount of concentrated fodder with high starch content has been included in the diet of these animals. These fodders are a source of energy for the cow, but the microorganisms of the rumen are also actively use it, forming a large amount of acid, which lowers the pH of the rumen and suppresses (sometimes even kills) the vital activity of the micro biota of the proventriculus. An important condition for the formation of lactic acid in the rumen is the presence of oxygen.Addition to the cows’ diet of live yeast helps to reduce the amount of oxygen in the rumen, which, in turn, inhibits the development of lactic acid bacteria and the synthesis of lactic acid by them. On the other hand, anaerobic environment in the proventriculus actively develops cellulose- lytic microorganisms.Many studies around the world have been devoted to the use of live yeast in feeding of cows. Most of them have been carried out at cows during the period of thermal stress. Part of the research covers only the period of early lactation. Almost all researchers note an increase in the milk productivity of cows; however, a relatively small number of publications are devoted to the problem of milk quality changes under the actions of these microorganisms.The purpose of this study was to investigate changes in the chemical composition and milk qualitative indicators of cows, which received in the diet the probiotic preparations of live yeast of different manufacturers.To study the effects of living yeast preparations in the diet on milk qualitative indicators and productivity of cows, they have conducted a scientific and economic experiment under the conditions of the educational and production center of the Bila Tserkva National Agrarian University. During the experiment, the chemical composition of milk, its density, acidity, bacterial insemination and the number of somatic cells have been investigated.For conducting of the scientific and economic experiment under the conditions of the farm, 30 cows of Ukrainian black-and-white milk breed were selected, from which with the group method three groups were formed: one control group and two experimental ones. The cows of the control group did not consume probiotic preparations, animals of the 2nd experimental group were fed with LevuSell SC at a dose of 1 g/head/day, and the third experimental group – ActiSaf Sc-47 in the amount of 5 g/head/day. The experiment lasted 305 days.The quality of milk has great importance, because it is a raw material for the further processing and production of a variety of dairy products, the main consumers of which are children. The current
牛首先是反刍动物。它的胃肠道被设计用来消化大量的结构纤维。遗传学、育种、饲养、饲养和挤奶方面的现代技术进步使奶牛能够获得超过10000公斤的泌乳乳,但只有在这些动物的饮食中加入大量高淀粉含量的浓缩饲料,才能实现这一目标。这些饲料是奶牛的能量来源,但瘤胃的微生物也在积极利用它,形成大量的酸,降低瘤胃的pH值,抑制(有时甚至杀死)前胃微生物群的重要活动。瘤胃中形成乳酸的一个重要条件是存在氧气。在奶牛的日粮中添加活酵母有助于减少瘤胃中的氧气量,从而抑制乳酸菌的发育和乳酸的合成。另一方面,在腺胃的厌氧环境中,纤维素分解微生物活跃发育。世界各地的许多研究都致力于在奶牛饲养中使用活酵母。大多数都是在奶牛处于热应激时期进行的。部分研究只涉及早期哺乳期。几乎所有的研究人员都注意到奶牛的产奶能力有所提高;然而,相对较少的出版物专门研究在这些微生物作用下牛奶质量变化的问题。本研究的目的是调查奶牛的化学成分和牛奶质量指标的变化,这些奶牛在饮食中接受了不同制造商的活酵母益生菌制剂。为了研究日粮中活酵母制剂对奶牛乳汁质量指标和生产力的影响,他们在Bila Tserkva国立农业大学教育和生产中心的条件下进行了一项科学和经济实验。在实验过程中,对牛奶的化学成分、密度、酸度、细菌受精和体细胞数量进行了研究。为了在农场条件下进行科学经济实验,选择了30头乌克兰黑白奶品种的奶牛,用分组法从中组成三组:一个对照组和两个实验组。对照组的奶牛不食用益生菌制剂,第二实验组的动物以1g/头/天的剂量喂食LevuSell SC,第三实验组——ActiSaf SC-47以5g/头/日的剂量喂食。实验持续了305天。牛奶的质量非常重要,因为它是各种乳制品的进一步加工和生产的原料,而这些乳制品的主要消费者是儿童。畜牧业的现状迫使牛奶加工企业长途运输牛奶(数百公里)。这就是为什么在奶牛的饮食中引入任何具有不同目的的饲料添加剂都不应对牛奶质量产生负面影响。在奶牛饲养中使用不同的活酵母制剂会影响牛奶的化学成分。在各组奶牛乳汁中的血糖和蛋白质含量指标几乎不变的情况下,第2和第3实验组动物乳汁中粗脂肪含量的增加引起了关注。显然,这种变化是由于微生物群的增加和消化的正常化,瘤胃中纤维(结构碳水化合物)消化率增加的结果。实验组奶牛乳汁中脂肪浓度的增加使干物质含量和乳汁密度略有增加。牛奶的酸度是新鲜度及其储存能力的指标。根据这个标准,所有组的动物都是平等的。乳制品行业最大的问题之一是原料奶中存在大量微生物,由于挤奶、储存、运输牛奶等过程中的各种违规行为,这些微生物被输送到那里。给实验组的奶牛喂食活酵母必须影响牛奶的细菌定殖率,但即使是这种微小的变化也朝着改进的方向发生了。因此,根据细菌受精指标,第二实验组的奶牛的乳汁与对照类似物相比减少了3.1%。根据该标准,对照动物比第三实验组的奶牛的优势为4.0%。奶牛活酵母日粮中最大的变化是通过牛奶中体细胞的含量来测量的。
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引用次数: 0
Peculiarities of nutrient digestibility of rations of pregnant ewes with the additional introduction of cobalt 添加钴后怀孕母羊饲料营养物质消化率的特点
Pub Date : 2018-12-27 DOI: 10.33245/2310-9289-2018-145-2-24-31
V. Mykytyuk, T. Vasilenko, O. Orischuk, S. Tsap, I. Porotikova
The results of studies on the effect of various doses of cobalt with an optimized level of sulfur in rations of Askanian meat-and-wool ewes on digestibility of nutrients of consumed feed and digestibility of the studied mineral elements are presented.It was established that the level of nutrient digestibility of rations was higher in ewes of the experimental groups, which, to the generally accepted norm, were additionally injected with cobalt chloride in the amount of 10 %, which was equal 0.825 mg, 20 % – 0.900 mg, and 30 % – 0.975 mg per head per day .As a result of the conducted research, it was found that the ewes of the second experimental group differed in the highest indices of digestibility of nutrients of the ration, which increased the rate of cobalt by 10 %.The additional introduction of 20 % and 30 % cobalt to the basic ration did not contribute to a further increase in the digestibility factors of nutrients in the body of ewes of the experimental groups.The research has shown that the uptake of cobalt by experimental ewes increased as this element increased in the rations. Thus, 0.31 mg of cobalt was assimilated by the ewes of the second experimental group, which is 0.06 mg or 20.0 % higher compared to the control group; to the third-group of ewes by 0.07 mg or 28.0 % and to the fourth-group ewes by 0,08 mg or 32.0 % compared with analogues of the control group. However, when calculating these indicators in relative terms, it was established that the highest percentage of cobalt absorbed from that received was in the ewes of the second experimental group. Their advantage relative to the control group was 4.60 % (P <0.01), and III and IV experimental groups – 2.11 and 0.19 absolute percent.
本文介绍了在阿斯卡尼亚肉羊毛母羊日粮中添加不同剂量的钴和最佳硫水平对所消耗饲料营养物质消化率和所研究矿质元素消化率的影响。成立口粮的养分消化率水平更高的母羊实验团体,普遍接受的规范,另外注射氯化钴的量为10%,等于0.825毫克,- 0.900毫克,20%和30%,人均0.975毫克/天。as的结果进行研究,发现第二个实验组的母羊不同营养物质的消化率最高指数的定量,使钴的生成速率提高了10%。在基础日粮中添加20%和30%的钴对试验组母羊体内营养物质的消化率没有进一步提高。研究表明,试验母羊对钴的吸收随饲料中钴元素的增加而增加。实验2组母羊吸收钴量为0.31 mg,比对照组提高0.06 mg或20.0%;与对照组的类似物相比,第三组母羊减少0.07 mg或28.0%,第四组母羊减少0.08 mg或32.0%。然而,在相对计算这些指标时,可以确定的是,从接收的钴中吸收钴的百分比最高的是第二实验组的母羊。相对于对照组的优势为4.60% (P <0.01),相对于ⅲ、ⅳ试验组的优势分别为2.11%、0.19%。
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引用次数: 0
Influence biologically of active substances to amaranth on composition of lipids in organism of quails 苋菜活性物质对鹌鹑机体脂质组成的生物学影响
Pub Date : 2018-12-27 DOI: 10.33245/2310-9289-2018-145-2-46-53
N. Ponomarenko, S. Tsehmistrenko, O. Tsekhmistrenko, V. Polishchuk, S. Polishchuk
There were investigated the level of common lipids and the ratio of their individual classes – phospholipids, monoacylglycerols, diacylglycerols and triacylglycerols, free and esterified cholesterol, unesterified fatty acids in the 6–8 weeks old quails pancreas (the period of laying eggs formation) under condition of nitrates influence and feeding of mixed fodder with the addition of amaranth seeds.The body's response to nitrate loading and the feeding of amaranth seeds was evaluated according to biochemical parameters in the pancreas, one of the largest glands in the body. For laboratory experiments there were used quails by Pharaon breed. Quails were divided into three groups of 60 heads in each, fed the standard feed, access to feed and water was free. The birds of the first group served as control, the birds of the second group, in order to model the stress state, water was dispensed with sodium nitrate at a dose of 0.5 g / kg of body weight. Birds of the third group were fed a mixed fodder with seeds of amaranth at the rate of 10 % of the mass of mixed fodder on the background of nitrate loading. Decapitation was performed under etheric anesthesia and the pancreas was taken from a 6-week-old birds (the beginning of the laying eggs period). In the extract of the pancreas, the content of total lipids and their individual classes were were srudied and expressed as a percentage of the total number of lipids. For identification of lipids on a chromatographic plate, the standard preparations of individual lipids from Sigma (USA) were used.The obtained results indicate that nitrate loading leads to a decrease in the common lipids content in the quail pancreas tissues. Thus, their number in the 6-week-old age is reduced by 37.0 % (p<0.05), and in the 8-week-old period – by 22.7 % (p<0.05) compared with the control group. Lipids perform structural and energy functions, as well as determine the important role for the adaptation of the organism to stress factors. Due to the long nitrate influence and due to the unequal functionality of the antioxidant systems of the body, protective mechanisms are violated, which leads to the occurrence of pathological processes. At the same time there are certain changes in the ratio of individual classes of lipids. In particular, the content of unesterified fatty acids (UFA) in 6-week-old poultry decreases by 13.3 % (p<0.05) and 8-week –12.4 % (p<0.05) compared to control. The level of UFA in tissues and serum is related to the energy supply of the bird organism and characterizes the activity of lipolysis processes and their mobilization from fat depots. The decrease in the tissues of the number of UFA indicates their intensive use in energy processes.Feeding of mixed fodder with seeds of amaranth against the background of nitrate influence causes changes in the total content of lipids and the ratio of their individual classes in pancreatic tissue compared with control and 2nd group. Thus the poultry, which was fe
研究了常见脂质的水平及其各自类别的比例——磷脂、单酰基甘油、二酰基甘油和三酰基甘油、游离胆固醇和酯化胆固醇,硝酸盐影响下6-8周龄鹌鹑胰腺(产卵期)中的未酯化脂肪酸和添加苋籽的混合饲料喂养条件。根据胰腺(人体最大的腺体之一)的生化参数来评估人体对硝酸盐负荷和苋籽喂养的反应。实验室实验使用了法老品种的鹌鹑。鹌鹑被分为三组,每组60头,喂食标准饲料,免费获得饲料和水。第一组的鸟作为对照,第二组的鸟,为了模拟应激状态,用水以0.5g/kg体重的剂量分配硝酸钠。在硝酸盐负荷的背景下,以混合饲料质量的10%的比例给第三组的鸟类喂食含有苋籽的混合饲料。在乙醚麻醉下进行滗析,并从6周大的鸟身上取下胰腺(产卵期开始)。在胰腺提取物中,研究了总脂质的含量及其各个类别,并将其表示为脂质总数的百分比。为了在色谱板上鉴定脂质,使用来自Sigma(USA)的单个脂质的标准制剂。结果表明,硝酸盐负荷导致鹌鹑胰腺组织中常见脂质含量下降。因此,与对照组相比,6周龄儿童和8周龄儿童的数量分别减少了37.0%(p<0.05)和22.7%(p<0.05)。脂质具有结构和能量功能,并决定生物体适应应激因素的重要作用。由于长期的硝酸盐影响和身体抗氧化系统的不平等功能,保护机制受到侵犯,从而导致病理过程的发生。同时,不同类别脂质的比例也有一定的变化。特别是,与对照组相比,6周龄家禽的未酯化脂肪酸(UFA)含量下降了13.3%(p<0.05),8周龄家禽下降了12.4%(p<0.05)。组织和血清中UFA的水平与鸟类机体的能量供应有关,并表征脂肪分解过程的活性及其从脂肪库中的动员。UFA数量在组织中的减少表明其在能量过程中的密集使用。与对照组和第二组相比,在硝酸盐影响的背景下喂食含有苋籽的混合饲料会导致胰腺组织中脂质的总含量及其各个类别的比例发生变化。因此,与第二组相比,在6周龄的家禽中,喂食混合的苋籽和硝酸盐影响的家禽的总脂质含量增加了2.0倍(p<0.05),在8周龄的禽肉中,总脂质含量提高了2.2倍(p>0001),与对照组相比,8周龄家禽的总脂含量增加了1.5倍(p=0.05)。同时,与对照组相比,单酰基和二酰基甘油的含量降低了15.3-20.9%(p<0.05),而三酰基甘油增加了45.5%(p<0.05)。与第二组相比,给鹌鹑喂食苋籽会使8周龄的鹌鹑胆固醇酯含量增加1.3倍(p<0.05),它们在6-8周龄鸟类胰腺中的含量增加了20,9-36.7%(p<0.05)。这些模式表明,胰腺组织中脂肪分解和储备脂质积累的抑制作用受到抑制。胆固醇酯数量的增加表明它们在鸟类生物体中的活性用途,这是由于在苋种子的生物活性物质,特别是角鲨烯的影响下胆固醇的酯化和水解过程的变化。可以假设,当进入人体时,苋籽角鲨烯最初会导致胰腺中胆固醇含量增加,因为它是胆固醇合成的前体。因此,胆固醇含量的增加激活了酰基辅酶A胆固醇酰基转移酶,而胆固醇酯的含量开始增加。值得注意的是,胆固醇酯中的类固醇核心更耐氧化,可能因此,细胞以酯的形式更好地储存胆固醇。在硝酸盐负荷的背景下进食的阿玛兰鸟阻止了总脂质含量的降低,并有助于胰腺中储备脂质的积累。 这可以通过苋红多不饱和脂肪酸的含量来解释,苋红与维生素和微量元素一起调节鸟类体内的脂质代谢。因此,硝酸盐的影响导致6-8周龄鹌鹑胰腺组织中脂质的总含量降低,未酯化脂肪酸的数量减少,这表明它们在能量过程中的使用强度很大。在硝酸盐影响的背景下,在产蛋期用含苋籽的混合饲料喂养家禽,有助于将总脂质含量提高到对照组的水平。与此同时,各个类别的脂质的比例也发生了变化,表现为单酰基和二酰基甘油含量的降低和胆固醇酯含量的增加。关键词:磷脂,单酰基甘油,二酰基甘油,三酰基甘油,游离胆固醇,胆固醇酯,未酯化脂肪酸,胰腺,硝酸盐,鹌鹑,苋。
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引用次数: 0
The influence of genotypic factors on the forming of the economic use of values of ukrainian bleck and wheat milk breeds 基因型因素对乌克兰大麦和小麦乳品种经济利用价值形成的影响
Pub Date : 2018-12-27 DOI: 10.33245/2310-9289-2018-145-2-06-13
Nataliia Ihorivna Klopenko, R. Stavetska, M. Bushtruk, I. Starostenko, O. Babenko
The evaluation of dairy cows` exterior plays a key role in the system of breeding because well-defined breed typology and high exterior evaluation cause high production efficiency. The objective of the study was to evaluate the exterior of the cows in Ukrainian Black and White dairy breed, to analyze the effectiveness of the selection of first-calf cows according to the body measurements and to study the influence of genotype factors on the formation of the exterior.For research purpose animal groups were formed on the principle of analogues, taking into account the part of Holstein inheritance, parentage, linear belonging, and age. Depending on the part of Holstein inheritance researched livestock was divided into three groups: 75,0-87,4 %, 87,5-99,9 % and 100 %.As a result of our own research it has been established that simultaneously with an increasing of the part of Holstein inheritance the body measurements increase too. It indicates about the improving effect of the Holstein breed on the formation of the exterior in the Ukrainian black-and-white milk breed. The first-calf cows with part of Holstein inheritance 100 % have advantages over the cows with the same age with part of Holstein inheritance 75,0-87,4 % with the withers height – 4 cm (P < 0,001), sacrum height – 4 cm (P <0.001), depth of chest – 1 cm, width of chest – 1 cm, width of hips – 1 cm, length of thoracic – 3 cm, chest girth – 2 cm, width of pins – 1 cm (P <0,001); the advantage over the first-calf cows with part of Holstein inheritance 87,5-99,9 % was 3 cm in withers height, 2 cm in sacrum height and 1 cm in all other cases.An increasing of the part of Holstein inheritance is accompanied by an increasing of milk yield, milk fat and milk protein. The higher milk yield had cows with the part of Holstein inheritance 100 % – by 294 kg compared to cows of the same age with the part of Holstein inheritance 87,5-99,9 % and by 2017 kg (P < 0,001) compared to cows of the same age with the part of Holstein inheritance 75,0-87,4 %. Fat content in milk of cows with the part of Holstein inheritance 100 % was lower by 0,02 % compared to cows with the part of Holstein inheritance 87,5-99,9 % and 0,21 % (P < 0,001) compared to cows with the part of Holstein inheritance 75,0-87,4 %, it was expected – the higher milk yield, the lower fat content in milk.The effectiveness of the simultaneous complex selection of dairy cattle on several grounds depends on the existence of correlation between them. The correlation between the body measurements of cows and 305-milk yield is positive, but varied in strength. The average strength of the correlation is observed between the milk yield and withers height (r = +0,233, P < 0,001), milk yield and length of thoracic (r = +0,332, P < 0,01), in other cases, the strength of the correlation is weak, but with sacrum height (P < 0,01), chest girt (P < 0,01) and depth of chest (P < 0,05) is significant.One-factor dispersion analysis was carried out to deter
奶牛的外部评价在育种体系中起着关键作用,因为明确的品种类型和高的外部评价导致了高的生产效率。本研究的目的是评估乌克兰黑白奶牛品种中奶牛的外观,根据身体测量结果分析第一头小牛的选择效果,并研究基因型因素对外观形成的影响。出于研究目的,动物组是根据类似物的原则形成的,考虑了荷斯坦遗传、亲子关系、线性归属和年龄。根据荷斯坦遗传的部分,研究的牲畜分为三组:75,0-87,4%,87,5-99,9%和100%。根据我们自己的研究结果,已经确定,随着荷斯坦遗传部分的增加,身体测量也会增加。说明荷斯坦品种对乌克兰黑白乳品种外观形成的改善作用。部分荷斯坦遗传100%的第一头小牛比部分荷斯坦继承75,0-87,4%的同龄奶牛具有优势,其肩高-4 cm(P<0.001),骶骨高-4 cm,胸部深度-1 cm,胸部宽度-1 cm,臀部宽度1 cm,胸部长度3 cm,胸围2 cm,胸针宽度1 cm(P<0.0001);与部分具有荷斯坦遗传的第一头小牛相比,87,5-99,9%的优势是枯萎高度为3cm,骶骨高度为2cm,在所有其他情况下为1cm。荷斯坦遗传部分的增加伴随着产奶量、乳脂和乳蛋白的增加。荷斯坦部分遗传的奶牛的产奶量比荷斯坦部分继承的同龄奶牛高出100%——294公斤,比荷斯坦遗传的同龄奶牛提高87,5-99,9%,到2017公斤(P<0.001)。与荷斯坦部分遗传的奶牛相比,荷斯坦部分继承100%的奶牛的乳汁中的脂肪含量降低了0,02%(P<0001),与荷斯坦遗传部分为75,0-87,4%的奶牛相比(P<0.001),产奶量越高,乳汁中的脂素含量就越低。基于多种理由同时对奶牛进行复杂选择的有效性取决于它们之间是否存在相关性。奶牛的身体测量值与305产奶量之间的相关性是正的,但强度不同。产奶量与肩颈高度(r=+00233,P<0001)、产奶量和胸部长度(r=+0.032,P<0,01)之间的平均相关性强度较弱,但与骶骨高度(P<0,0 1)、胸围(P<0.01)和胸部深度(P<0.05)之间的相关性强度显著。进行单因素离散分析,以确定基因型因素对活体重和体重测量的决定水平。根据研究结果,公牛的出身对其第一个小女儿的活重的影响为72.8%,对身体测量的影响为-31,5-91.3%,系属的影响分别为81.0%和36,1-69.4%,荷斯坦遗传部分的影响分别是26.3%和15,9-39.5%。所有基因型因素对第一头小牛的活重(P<0,05…0001)、肩颈高度(P<0.05…0,01)、胸部宽度(P<0.01…0001)和胸针宽度(P<0,05…0.001)都有显著影响。因此,对第一头母牛的身体测量和活重的最大影响来自公牛,就在属于的线下方,最低的——荷斯坦遗产的一部分,但在所有情况下都相当高。已经确定,为了改善奶牛的外观,将推广使用具有理想的活重指标和女儿体重测量指标的公牛和品系,以及具有良好外观特征的高荷斯坦遗传率的动物。为了进行有效的选择,需要使用检测到的产奶量和身体测量之间的相关性。关键词:外观,身体测量,荷斯坦遗传的一部分,公牛的起源,牛奶产量。
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引用次数: 1
Estimation of 137Cs and 90Sr accumulation in the organism of dairy cows in the radioactive contaminated agro landscapes of the Central Forest-steppe in the remote period of the Chernobyl catastrophe 切尔诺贝利灾难早期中央森林草原放射性污染农业景观中奶牛体内137Cs和90Sr积累的估算
Pub Date : 2018-12-27 DOI: 10.33245/2310-9289-2018-145-2-62-71
O. Rozputnyi, V. Herasymenko, I. Pertsovyi, V. Skyba, M. Saveko
Because of the Chernobyl catastrophe, almost all the territory of Polissya and a significant part of the Forest-Steppe south of Kyiv suffered radioactive contamination. More than three decades have passed since the Chernobyl accident, but despite the time since the disaster, the problem of radioactive contamination is still very relevant. In radioactive contaminated agro landscapes, plant fodder becomes a source of 137Cs and 90Sr in the body of cows. In the organism of an animal, radionuclides of cesium mainly accumulate in muscle tissue, and strontium in bone and excreted with milk, urine and feces. Milk plays an important role in human nutrition, which necessitates constant monitoring of the radio ecological situation, estimates of the accumulation of 137Cs and 90Sr, and the determination of the laws governing the transition of these radionuclides into products.The aim of the research was to evaluate the supply of 137Cs and 90Sr with feed in the body of dairy cows and the accumulation of these radionuclides in milk and barnyard manure mass in radioactive contaminated agricultural landscapes of the Central Forest Steppe. The research was carried out at LLC "Agro-Leader Ukraine" Ltd. and "Nadiya" Ltd and on the peasants’ plots of Yosypivka, Tarasivka villages of the Bila Tserkva district of the Kyiv region who suffered from the radioactive contamination because of the Chernobyl catastrophe.Samples of soils, fodder, milk and barnyard manure mass for conducting research were selected. The activity of 137Cs and 90Sr was determined at the USM "Gamma Plus U" with the "Progress 2000" software in the laboratory of the Bila Tserkva NAU, department of life safety. The activity of 137Cs was determined by the method of scintillation gamma spectrometry in a Marinelli vessel of volume 1L in native samples or after their physical concentration, and 90Sr – after radiochemical isolation by scintillation beta spectrometry.The results of the studies indicate that the content of 137Cs and 90Sr in feed is directly proportional to the level of soil contamination by these radionuclides. At the same time, the least accumulated 137Cs and 90Sr in the green mass of corn, and most of all in the green mass of alfalfa and oats mixture. The intensity of the accumulation of 90Sr in the green mass of corn is 20 times, the alfalfa – in 1,5-2 times, the use of oatmeal mixture – 4 to 6 times higher than 137Cs.It has been established that the concentration of 137Cs and 90Sr in milk of cows is directly proportional to their activity in the diet. In the daily hopes of milk from cows from the diet 7,6 ± 0,55 % of 137Cs and 1,7 ± 0,42 90Sr passed from their activity in the diet. The coefficient of transition of 137Cs in 1 liter of milk on average was 0.76 % and 0.17 % 90Sr. The activity of 137Cs and 90Sr in milk does not exceed the permissible levels. At the same time, in the milk of households, the activity of 137Cs and 90Sr in the spring-summer period was two to three times highe
由于切尔诺贝利灾难,波利西亚几乎所有领土和基辅以南森林草原的大部分地区都遭受了放射性污染。切尔诺贝利事故已经过去三十多年了,但尽管灾难已经过去了,放射性污染的问题仍然非常重要。在受放射性污染的农业景观中,植物饲料成为奶牛体内137Cs和90Sr的来源。在动物体内,放射性核素铯主要积聚在肌肉组织中,放射性核素锶主要积聚在骨骼中,并随奶、尿和粪便排出体外。牛奶在人类营养中起着重要的作用,这就需要不断监测放射性生态状况,估计137Cs和90Sr的积累,并确定这些放射性核素向产品转变的规律。本研究旨在评价中央森林草原受放射性污染的农业景观中,137Cs和90Sr随饲料在奶牛体内的供应,以及这些放射性核素在牛奶和粪肥中的积累。研究是在“agroleader乌克兰”有限责任公司和“Nadiya”有限责任公司以及基辅地区Bila Tserkva区的Yosypivka、Tarasivka村的农民地块上进行的,这些农民因切尔诺贝利灾难而受到放射性污染。选取土壤、饲料、牛奶和厩肥等样品进行研究。137Cs和90Sr的活性是在Bila Tserkva NAU生命安全系实验室的USM“Gamma Plus U”上用“Progress 2000”软件测定的。137Cs的活度在体积为1L的Marinelli容器中或物理浓缩后用闪烁伽马能谱法测定,90Sr -的活度在放射化学分离后用闪烁β能谱法测定。研究结果表明,饲料中137Cs和90Sr的含量与土壤受放射性核素污染的程度成正比。同时,137Cs和90Sr的累积量在玉米青质量中最少,在苜蓿燕麦混合青质量中最多。90Sr在玉米青苗中的积累强度是137Cs的20倍,苜蓿的1.5 ~ 2倍,燕麦混合物的使用强度是137Cs的4 ~ 6倍。奶牛乳中137Cs和90Sr的浓度与它们在日粮中的活性成正比。在日粮中奶牛的日产奶量中,有7,6±0,55 %的137Cs和1,7±0,42 sr来自它们在日粮中的活动。137Cs在1升牛奶中的转变系数平均为0.76%,90Sr为0.17%。牛奶中137Cs和90Sr的活性没有超过允许水平。同时,由于在天然牧场放牧奶牛,农户牛奶中137Cs和90Sr的活性在春夏期比秋冬期高2 ~ 3倍,而天然牧场的土壤污染程度远高于耕地。牛奶中137Cs和90Sr活性形成的决定因素是这些放射性核素随日粮饲料的量,这取决于饲料的组成和种植饲料作物的土壤污染的密度。研究表明,牛体内来自植物性食物的137Cs和90Sr的主要比例(高达90%)被转化为粪肥团。牛场粪中137Cs和90Sr的积累系数为0.87。牛粪块进入土壤后,成为土壤二次污染的来源,促进137Cs和90Sr在农业景观中的迁移和再分配。在放射性污染地区获得的牛粪块只能在生产牛粪块的农场内使用。研究表明,在受放射性污染的森林草原地区可以种植饲料作物,并且可以不受任何限制地获得牛奶。关键词:农业景观,放射性污染区,放射性核素,137Cs, 90Sr,草,奶牛,牛奶,牛粪,森林草原带
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Tekhnologiia virobnitstva i pererobki produktiv tvarinnitstva
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