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Effect of Salt and Inhibitoron the Isolation, Purification and Characterization of α -Amylase from Aspergillusniger Produced from Pigeon Pea 盐和抑制剂对鸽豆黑曲霉α -淀粉酶的分离纯化及特性的影响
Pub Date : 2021-12-04 DOI: 10.55124/jfsn.v1i1.155
A. O. R.
α-Amylase an industrially used enzyme can be obtained from Aspergillusniger and can be produced from food sources such as pigeon pea. α-Amylase was produced from Aspergillusniger isolated from pigeon pea, purified and characterized. This process was achieved using ammonium sulphate, ion exchange DEAE column and gel filtration (Sephadex A-50 and sephadex G-100) chromatography. The effect of salt and inhibitor was determinedAmmonium sulphate precipitation results showed that the highest specific α-amylase activity was (1.01 U/ml. mg) obtained at 11.27% saturation level, with a purity of 1.81-fold of the crude extract and yielding 1.00%. Further purification using gel filtration increased the enzyme purity and yielding 8.94-fold relative to the crude extract 3.01% and yielding Specific activity after purification was 4.99 U/mg. The effect of salts on α-Amylase activity increased to 258.09% when in MgSO4, while decreased to 7.71% and 21.07% when in MnSO4 and CuCl2 respectively and yielded no result when in PbNO3.Its reaction with chemical inhibitors such as Bromosuccinimide was activated to 136.465% and was inhibited at Mercaptoethanol to 0%. All these were determined using a visible spectrophotometer with an absorbance of 540nm, against the control that contains 100µL of the enzyme and 100µL of 1% starch solution.Therefore α -amylase produced from Aspergillusniger can be exploited for potential usage for industrial applications of enzymes in a wide range of production and its application in food processing.
α-淀粉酶是一种工业上使用的酶,可以从黑曲霉中获得,也可以从鸽豆等食物来源中生产。α-淀粉酶是从鸽豆中分离得到的黑曲霉,并对其进行了纯化和鉴定。该工艺采用硫酸铵、离子交换DEAE柱和凝胶过滤(Sephadex A-50和Sephadex G-100)色谱法实现。测定了盐和抑制剂对α-淀粉酶的影响。硫酸铵沉淀结果表明,α-淀粉酶的最高特异性活性为(1.01 U/ml)。Mg)在11.27%饱和水平下得到,纯度为粗提物的1.81倍,得率为1.00%。纯化后的酶纯度比粗提物提高了8.94倍,比活性为4.99 U/mg,比活性为3.01%。盐对α-淀粉酶活性的影响在MgSO4中增加到258.09%,在MnSO4和CuCl2中分别降低到7.71%和21.07%,在PbNO3中没有影响。其与溴丁二酰亚胺等化学抑制剂的反应被激活至136.465%,巯基乙醇的反应被抑制至0%。所有这些都是用可见光分光光度计测定,吸光度为540nm,对照组含有100µL的酶和100µL的1%淀粉溶液。因此,黑曲霉生产的α -淀粉酶具有广泛的工业应用潜力,在食品加工中的应用也十分广泛。
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引用次数: 0
Total arsenic, arsenic species, and trace elements in crop and vegetable grown in areas irrigated with arsenic contaminated water in Bangladesh and West Bengal-India 在孟加拉国和西孟加拉-印度用砷污染的水灌溉的地区种植的作物和蔬菜中的总砷、砷种类和微量元素
Pub Date : 2021-10-25 DOI: 10.55124/jfsn.v1i1.121
U. Chowdhury
Article history: Received 20210130 Received in revised form 20210425 Accepted 20210425 Available online 20210425 In this study, tube well water, soil, crop, and vegetable were collected from agricultural field where irrigated with arsenic contaminated water. Estimation of total arsenic and other metals and metalloids in soil, vegetable, and paddy (rice & husk) samples by using ICP-MS after microwave digestion. But arsenic species in paddy (rice + husk), rice, husk, and vegetable by IC-ICP-MS after TFA extraction.
在本研究中,我们收集了用砷污染水灌溉的农田的管井水、土壤、作物和蔬菜。微波消解后ICP-MS法测定土壤、蔬菜和稻壳样品中总砷和其他金属及类金属。但经TFA提取后,用IC-ICP-MS测定了水稻(水稻+稻壳)、水稻、稻壳和蔬菜中的砷种类。
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引用次数: 1
Food Chain Arsenic: Additional body burden on health of arsenic 食物链砷:砷对身体健康的额外负担
Pub Date : 2021-10-25 DOI: 10.55124/jfsn.v1i1.123
U. Chowdhury
Introduction     Arsenic: The king of poisons, the poisons of kings, and the bane of investigators1. The IARC2 has classified arsenic as a group 1 human carcinogen. Chronic exposure to inorganic arsenic can cause cancerous2-5 and non-cancerous health hazards6,7 in humans. Arsenic can get entry into the human body via drinking water, eating food, inhaling dust, and/or ingesting soil.   In arsenic affected areas of West Bengal-India and Bangladesh huge quantity of arsenic is falling on agricultural land. A study in West Bengal-India reported that in a 201 km2 area of the Deganga block in the arsenic-affected district of North 24-Parganas, 6.4 tons of arsenic is falling on agricultural land in one year from 3200 contaminated tube wells for agricultural irrigation8. They expect tons of arsenic is coming with underground water in the arsenic affected areas of West Bengal-India and Bangladesh and falling on irrigated land. Thus, it is expected arsenic is entering the food chain.           Figure. Using tube well water for agricultural irrigation.   Rice and vegetable are the staple food for poor villagers of West Bengal, India and Bangladesh. This is true for the villagers in Kolsur gram-panchayet (G.P.) in Deganga block of North 24-Parganas district, West Bengal-India, where a group of researchers studied for arsenic in soil, rice, and vegetables from fields cultivated with arsenic contaminated water. From the results of total arsenic (drinking water + rice + vegetables + Pantavat + water added for food preparation) body burden to North Kolsur villagers [1185.0 µg for per adult per day and 653.2 µg for per child (around 10 years) per day], as the amount of arsenic coming from rice, vegetables, and water added for Pantavat and food preparation  is 485 µg i.e., 41% of total for adult and 253.2 µg i.e., 38.8% for child and from rice and vegetable 285 µg i.e., 24% of total for adult and 153.2 µg i.e., 23.4% for child9-11. Their findings show most of the arsenic coming from food is inorganic in nature10. They reported that 95% and 5% of the arsenic are inorganic arsenic and methylated arsenic in rice, and 96% and 4% are inorganic arsenic and methylated arsenic in vegetables, respectively10.     According to WHO12 1.0 µg of inorganic arsenic per day may give rise to skin effects within a few years. It has been estimated that based upon the current U.S. Environmental Protection Agency (EPA) standard of 50 µg/L, the lifetime risk of dying from cancer of the liver, lung, kidney, or bladder, from drinking 1 liter per day of water could be as high as 13 per 1000 persons13. Using the same methods, the risk estimate for 500 µgL of arsenic in drinking water would be 13 per 100 persons14. In its latest document on arsenic in drinking water, the U.S. National Research Council (NRC) concluded that exposure to 50 µg/L could easily result in a combined cancer risk15 of 1 in 100. Comparing to the WHO, EPA, and NRC document with arsenic burden to Kolsur vi
砷:毒药之王,国王的毒药,调查员的克星。国际癌症研究机构将砷列为1类人类致癌物。长期接触无机砷可对人类造成致癌2-5和非癌性健康危害6,7。砷可以通过饮用水、食用食物、吸入灰尘和/或摄入土壤进入人体。在西孟加拉邦、印度和孟加拉国受砷影响的地区,大量的砷落在农田上。西孟加拉邦-印度的一项研究报告称,在北24-帕尔加纳斯砷污染地区的德甘加区块201平方公里的区域内,每年有6.4吨砷从3200口受污染的农业灌溉管井中落到农田上。他们预计,在西孟加拉邦-印度和孟加拉国受砷影响的地区,数以吨计的砷将随着地下水流入灌溉土地。因此,预计砷正在进入食物链。数字利用管井水进行农业灌溉。大米和蔬菜是西孟加拉邦、印度和孟加拉国贫穷村民的主食。这对印度西孟加拉邦北24-帕尔加纳斯区德甘加街区的Kolsur gram-panchayet (G.P.)的村民来说是真的。在那里,一组研究人员研究了土壤、水稻和蔬菜中的砷含量,这些土壤、水稻和蔬菜都是用砷污染的水种植的。结果的总砷(饮用水+米饭+蔬菜+ Pantavat +水为准备食物添加)身体负担北Kolsur村民[1185.0µg /成人每天和每个孩子653.2µg(大约10年)每天),大量的砷来自米饭,蔬菜,和水添加Pantavat和准备食物485µg即成人总数的41%和253.2µg例如,38.8%的儿童和大米和蔬菜285µg。占成人总量的24%,153.2微克,即9-11岁儿童的23.4%。他们的发现表明,大部分来自食物的砷本质上是无机的。他们报告说,水稻中95%和5%的砷是无机砷和甲基化砷,蔬菜中96%和4%分别是无机砷和甲基化砷10。根据世界卫生组织12,每天摄入1.0微克无机砷可能在几年内引起皮肤效应。据估计,根据目前美国环境保护署(EPA)的50微克/升的标准,每天饮用1升水,一生中死于肝癌、肺癌、肾癌或膀胱癌的风险可能高达每1000人中有13人。使用同样的方法,饮用水中500 μ gL砷的风险估计为每100人13人14。美国国家研究委员会(NRC)在其关于饮用水中砷含量的最新文件中得出结论,接触50微克/升的砷很容易导致百分之一的癌症风险。与世界卫生组织、美国环保署和美国核管理委员会关于水和食物给科尔苏尔村民带来砷负担的文件相比,科尔苏尔村民患砷皮肤效应和癌症的风险是存在的。与世界范围内从食物中摄入的砷相比,科尔苏尔村民似乎也从食物和蔬菜中摄入了大量无机砷。科尔苏尔村是西孟加拉邦-印度和孟加拉国许多这样的村庄的一个例子。此外,砷灌溉水-富砷土壤系统的产品也进入远离污染地区的普通市场,甚至没有饮用砷污染水的人也可能从污染地区生产的食品中摄取砷。在西孟加拉-印度和孟加拉国,大米、蔬菜和其他产品从农村进入城市(包括西孟加拉-印度的加尔各答和孟加拉国的达卡),不能排除城市居民从受污染地区食用受砷污染产品的可能性。参考文献:Aposhian, h.v., Avram, m.d., Tsaprailis, G., Chowdhury, uk, 2006。砷:毒药之王,毒药之王,调查员的祸根(会议论文)。化学与毒物学。,第16卷,1680-1680。IARC(国际癌症研究机构)1987. 国际癌症研究机构关于人类致癌性风险评估的专著。致癌性的全面评估:IARC专著1-42的更新(增刊7).里昂,法国:国际癌症研究机构,第100-106页。NRC(国家研究委员会)。2001. 饮用水中的砷。1999年食水含砷报告的最新情况。华盛顿:国家科学院出版社。C.J陈,陈,老温,答,郭,T.L. 1992。由于饮用水中摄入无机砷,可能会导致肝、肺、膀胱和肾脏的癌症。Br。[j] .中国医学杂志(英文版),23(5):888-892。Rossman, t.g., Uddin, a.n., Burns, F.J. 2004。有证据表明亚砷酸盐在皮肤癌中是一种致癌物质。Toxicol。达成。医学杂志,1998,39(4):394-404。黄,Y.K曾,刘昀黄Y.L,杨M.H Chen C.J. 薛彦明,2007。台湾西南地区砷中毒高流行区居民的砷甲基化能力与高血压风险,中华毒物杂志。达成。药学杂志,18(2):135-182。曾志华,2007。无机砷的代谢和与人体慢性接触有关的非癌性健康危害。j .包围。生物学报,28,349-357。曼达尔,b.k., 1998。印度西孟加拉邦8个地下水砷污染地区60个区块中2个区块的砷问题现状(博士论文)。印度加尔各答Jadavpur大学乔杜里,英国,2001。孟加拉国四个地理形态区域的地下水砷污染状况(特别参考生物样品和农作物中的砷)(博士论文)。印度加尔各答Jadavpur大学乔杜里,英国,拉赫曼,m.m.,曼达尔,b.k.,保罗,洛德,D,巴苏,g.k.,昌达,c.r.,萨哈,k.c.,慕克吉,s.c.,罗伊,s.s.,达斯,R,凯斯,I,巴鲁阿,a.k.,帕里特,s.k., Quamruzzaman, Q和Chakraborti, D.地下水砷污染与印度西孟加拉邦和孟加拉国人民的痛苦。环境科学学报,2003,8(1):393-415。乔杜里,英国(2021)。印度西孟加拉邦用砷污染的水灌溉的地区种植的作物和蔬菜中的总砷、砷种类和微量元素(提交)。卫生组织(世界卫生组织),1981年。砷:环境卫生标准日内瓦,瑞士:世界卫生组织。史密斯,A.H.等人,1992。饮用水中的砷有致癌风险。环境与健康展望。97:259-267。史密斯,A.H.等人,1999。饮用水中砷致癌风险:对饮用水标准的影响。见:第三届砷暴露与健康影响国际会议论文集,1998年7月12-15日,圣地亚哥,Elsevier科学有限公司,牛津,英国。页191 - 200。美国国家研究委员会,1999。饮用水中的砷。华盛顿特区,国家科学院出版社。
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引用次数: 0
Will Cultivated Meat Take Over The Food Industry? 人造肉会接管食品工业吗?
Pub Date : 2021-07-13 DOI: 10.55124/jfsn.v1i1.106
Suryakiran Navath
Ever since the corona virus pandemic began, a significant chunk of the world population has lost its life. But despite the enormous number of deaths the world has seen, its demand for food seems to be on the rise. The global health crisis has deteriorated the economies worldwide, causing people to lose their jobs at an unimaginable rate. With millions of people employed, the food insecurity graph is rapidly climbing. In October 2020, The UN’s Food and Agriculture Organization (FAO) reported that food insecurity impacts more than 2 billion people, citing an increase of 10 million from October 2019. Suffice to say that the demand for food is climbing, and studies suggest that it will continue to grow, forcing the food industry to feed 10 billion mouths by 2050. And with meat being the primary source of protein, and in general, food, relying on industrial animal agriculture for meat products is getting more and more unsustainable. That is why many food manufacturers have developed environmentally sustainable ways to produce meat in a lab without harming the animals. The meat produced in an artificial environment is cultivated, cell-based, slaughter-free, cultured, cell-cultured, or clean meat. And by the looks of the food market, it seems that cultured meat will take over the entire industry in the future. Cultivated Meat: The Science The science behind cultured meat is pretty simple; experts cut out stem cells from an animal under anesthesia. The procured sample is then placed with nutrients, growth factors, salts, and pH buffers and left to proliferate. The resulting product is slaughter-free meat. Although the process of cultivating faux meat is slow, the industry is beginning to flourish at a remarkable rate. Figure 1. Red meat steak with red chilies and black peppers Staggering Stats Forbes has reported that the global cultivated meat market is expected to grow $15.5m by 2021 and $20m by 2027, and nearly 35% of all meat available in the market by 2040 will be cell-based. According to another study conducted by the Institute of the Future in Palo Alto, cultivated meat will be a standard product in supermarkets by 2023. Despite being a relatively recent synthetic product, cultured meat seems to be going mass-market quite early on in its life. It was only four years ago when an American company created quite a buzz producing meat-less, cell-based meatballs. The Beginning of Cell-Based Meat Industry The California-based company Memphis Meats introduced cultured meatballs four years ago as an alternative to real meat. Since then, the company has been working on mega projects to lunch cell-based meaton a much larger level worldwide. Memphis Meats’ CEO, Uma Valeti, is hell-bent on providing the world with slaughter-free meat to reduce the risk of heart disease and offer an affordable meat-like meat alternative. His corporation is currently working on a pilot plant to produce beef, chicken, and duck on a mass scale. Memphis Meat is not the
自冠状病毒大流行开始以来,世界上有很大一部分人口失去了生命。但是,尽管世界上有大量的人死亡,但其对食物的需求似乎在上升。全球卫生危机恶化了世界各地的经济,导致人们以难以想象的速度失去工作。由于有数百万人就业,粮食不安全状况正在迅速攀升。2020年10月,联合国粮食及农业组织(粮农组织)报告称,粮食不安全影响了20多亿人,比2019年10月增加了1000万人。可以说,对食物的需求正在攀升,研究表明,它将继续增长,迫使食品工业到2050年养活100亿人。由于肉类是蛋白质的主要来源,一般来说,是食物的主要来源,依靠工业化畜牧业生产肉类产品变得越来越不可持续。这就是为什么许多食品制造商已经开发出环境可持续的方法,在实验室生产肉类而不伤害动物。在人工环境中生产的肉是培养的、基于细胞的、无屠宰的、培养的、细胞培养的或清洁的肉。从食品市场的情况来看,人造肉似乎将在未来占据整个行业。人造肉背后的科学原理很简单;专家在麻醉状态下从动物身上切除干细胞。然后将获得的样品与营养物、生长因子、盐和pH缓冲液一起放置,并使其增殖。最终的产品是没有屠宰的肉。虽然培育人造肉的过程很慢,但这个行业正开始以惊人的速度蓬勃发展。图1所示。《福布斯》报道称,到2021年,全球养殖肉类市场预计将增长1550万美元,到2027年将增长2000万美元,到2040年,市场上近35%的肉类将是细胞肉。根据帕洛阿尔托未来研究所进行的另一项研究,到2023年,人造肉将成为超市的标准产品。尽管人造肉是一种相对较新的合成产品,但它似乎很早就进入了大众市场。就在四年前,一家美国公司生产的无肉细胞肉丸引起了不小的轰动。四年前,总部位于加州的Memphis Meats公司推出了人造肉丸,作为真正肉类的替代品。从那时起,该公司一直致力于在全球范围内开展大型项目,以午餐细胞为基础的肉类。Memphis Meats的首席执行官乌玛·瓦莱蒂(Uma Valeti)一心想为世界提供免屠宰的肉类,以降低患心脏病的风险,并提供一种负担得起的类肉肉类替代品。他的公司目前正在进行大规模生产牛肉、鸡肉、鸭肉的试验工厂。孟菲斯肉类公司并不是市场上唯一的参与者;许多其他以细胞为基础的肉类制造公司也在努力扩大业务规模,以增加供应。旧金山的Artemys Foods、伯克利的Mission Barns和圣地亚哥的BlueNalu都在研究可持续的方式,为不断增长的世界人口提供包括鱼和鸭在内的养殖肉类。
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引用次数: 0
Traditional weight loss and dukan diets as to nutritional and laboratory results 传统减肥法和dukan饮食法的营养和实验结果
Pub Date : 2021-06-17 DOI: 10.55124/jfsn.v1i1.73
Patricia Naomi Sakae, Anita L R Saldanha, Antonio Helfenstein Fonseca, Henrique Trial Bianco, Luciano Monteiro Camargo, M. C. de Oliveira Izar, Ana Paula Pantoja Margeotto, A. L. Valera Gasparoto, Bruno Abdala, Tania Leme da Rocha Martinez, S.S. Miki Ihara
Background and Aims: Dukan diet, a popular diet with high content of protein and carbohydrate and fat restriction has been widely used for weight loss. We aimed to compare the effects of the Dukan diet with traditional low-calorie diet in nutritional, laboratory and vascular parameters in obese subjects. Methods and Results: Obese subjects classes I or II of both genders, aging 19 to 65 years were allocated into two groups: Traditional low-calorie diet (n=17) and Dukan Diet (n=17). Anthropometric, laboratory and vascular evaluations were performed at baseline, 3, 6 and 12 months. Body composition was evaluated by bioelectric impedance and endothelial function by flow-mediated dilation of the brachial artery, at same times. After 12 months, it was verified that Dukan diet was more effective (p<0.05) than traditional diet for: weight loss (-10.6 vs -2.9 kg), body mass index (-3.7 vs -1.1 kg/m2), waist circumference (-11.2 vs -2.1 cm), fat (-5.7 vs -2.0 kg) and lean mass (-4.8 vs 0.8 kg) and basal metabolic rate (-152 vs -28 cal). In Dukan diet group, improvement (p<0.05 vs baseline) was observed in triglyceride levels (172.40 to 111.90 mg/dL) and insulin resistance, based on HOMA-IR index (4.98 to 3.26). The glomerular  filtration rate decreased in this group after 3 months (132.50 to 113.80 mL/min) and no changes in flow-mediated dilation were observed throughout the study with both diets. Conclusion: Dukan diet was more effective than traditional diet for weight loss and laboratory parameters and without changes in endothelial function, in the 12-months follow-up of obese subjects. Introduction Low-carbohydrate diets have been one of the most recently used dietary therapies in patients with diabetes and obesity in clinical studies(1). Among them, in addition to carbohydrate restriction, fat restriction and high protein concentration, as in the Diet Dukan, has been widely used by the general population, aiming at weight loss. The Dukan diet is designed to reduce carbohydrate and fat intake in the first phase of the diet, with exclusive intake of protein, followed by another Three phases, with progressive and slow reintroduction of other nutrients such as fiber, carbohydrates and fats. In recent years, there has been increasing interest in the effectiveness of very low carbohydrate diets, called ketogenic diets, in the effectiveness of weight loss in order to combat obesity and cardiovascular disease risk(2). In this diet, ketone bodies are formed and they are used as an alternative energy source in the absence of glucose. Ketogenic diet promotes weight loss reducing appetite, increasing satiety and thermogenesis, due to the high protein consumption(3) affect hormones that control appetite, such as ghrelin and leptin(4) reduces lipogenesis and increases lipolysis(5,6) and gluconeogenesis(7). Replacing carbohydrates by proteins in the diet have been the aim of several studies but with inconsistent results. High protein intake has positive effects
BIA使用Biodynamics Model 450 TBW®仪器进行,配备便携式容积描记仪,并指导患者
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引用次数: 0
Breast feeding in refugee context: a scoping review 难民背景下的母乳喂养:范围审查
Pub Date : 2021-03-03 DOI: 10.55124/jfsn.v1i1.23
Dr. Juliana Vidal Vieira Guerra
IdentificationAuthorYear Country which study was conducted IdiomPopulation of the studyA1Jino e col.(14)2013GhanaEnglishRefugees from Kigali and RwandaA2Jessri e col.(15)2013CanadaEnglishMigrants e refugees from Middle EastA3Denis e col.(16)2014CanadaEnglishRefugees and migrants living in CanadaA4Gallegos e col.(17)2015AustraliaEnglishRefugees from Liberia, Sierra Leone, Burundi and Democratic Republic of CongoA5Jessri e col.(18)2015CanadaEnglishMigrantes e Refugiadas Árabes e IranianasA6Hufton e col.(19)2016EnglandEnglishRefugees in Liverpool and ManchesterA7Hunter-adams e col.(20)2016South AfricaEnglishMigrants And refugees from Democratic Republic of Congo Somalia e ZimbabweA8Woldeghebrie col.(21)2017GhanaEnglishLiberian Refugees
身份识别作者年份进行研究的国家idim研究人口1 jino e col(14)2013加纳英语来自基加利和卢旺达的难民2 jessri e col(15)2013加拿大英语来自中东的移民3 denis e col(16)2014加拿大英语居住在加拿大的难民和移民4 gallegos e col(17)2015澳大利亚英语来自利比里亚、塞拉利昂的难民布隆迪和刚果民主共和国5杰斯里上校(18)2015加拿大英语移民和难民Árabes e伊朗人1赫夫顿上校(19)2016英国英语利物浦和曼彻斯特的难民7亨特-亚当斯上校(20)2016南非英语刚果民主共和国索马里和津巴布韦的移民和难民8沃尔德格布里上校(21)2017加纳英语利比里亚难民
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引用次数: 1
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Journal of Food Science and Nutritional Disorders
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