Diabetes mellitus (DM) is a disease that causes blood glucose to increase above normal (hyperglycemia), which results in an increased level of free radicals. Antioxidants can fight the effects of free radicals. The combination of purple sweet potato (PSP) and black soybean tempeh (BST) is expected to increase antioxidant levels. This study aims to determine antioxidants levels, phenolics, and flavonoids from PSP and BST extract as antidiabetic candidates. Extraction was carried out by the maceration method using ethanol. The antioxidant activity test was carried out using the DPPH method, phenolic determination using the Folin-Ciocalteau (F-C) addition method, and the determination of flavonoid levels using quercetin as a standard. The spectrophotometer determined the IC50 of antioxidants, phenol, and flavonoid at 517, 740, and 510 nm, respectively. The highest antioxidant activity test results were obtained from the combination of BST and PSP (1 : 3), the IC50 value was 50.6 µg/mL, and the total phenol was 45.21 mgGAE/g extract. The most considerable total flavonoid level was found in the combination of BST and PSP ratio (3 : 1), 358.805 mgQE/g of extract. Our result suggested that the combination of BST and PSP has high antioxidant content and can be used as an alternative to antidiabetics.
{"title":"The evaluation of total phenolic, flavonoid, and antioxidant activities of black soybean tempeh and purple sweet potato as antidiabetic candidate","authors":"Yuny Damayanti, H. Susanto, A. Gofur","doi":"10.1063/5.0052612","DOIUrl":"https://doi.org/10.1063/5.0052612","url":null,"abstract":"Diabetes mellitus (DM) is a disease that causes blood glucose to increase above normal (hyperglycemia), which results in an increased level of free radicals. Antioxidants can fight the effects of free radicals. The combination of purple sweet potato (PSP) and black soybean tempeh (BST) is expected to increase antioxidant levels. This study aims to determine antioxidants levels, phenolics, and flavonoids from PSP and BST extract as antidiabetic candidates. Extraction was carried out by the maceration method using ethanol. The antioxidant activity test was carried out using the DPPH method, phenolic determination using the Folin-Ciocalteau (F-C) addition method, and the determination of flavonoid levels using quercetin as a standard. The spectrophotometer determined the IC50 of antioxidants, phenol, and flavonoid at 517, 740, and 510 nm, respectively. The highest antioxidant activity test results were obtained from the combination of BST and PSP (1 : 3), the IC50 value was 50.6 µg/mL, and the total phenol was 45.21 mgGAE/g extract. The most considerable total flavonoid level was found in the combination of BST and PSP ratio (3 : 1), 358.805 mgQE/g of extract. Our result suggested that the combination of BST and PSP has high antioxidant content and can be used as an alternative to antidiabetics.","PeriodicalId":365153,"journal":{"name":"INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129944813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
High salt-tolerant extracellular protease is an enzyme that plays a crucial role in industries and requires high salt conditions. Some protease enzymes are not resistant to high salt conditions because they may denature under high salt concentrations. Halophilic microbes produce extracellular enzymes that are stable at high salts. This research has succeeded isolating several halophilic microbes capable of producing extracellular proteases from Bledug Kuwu, Grobogan, Central Java, Indonesia. Extracellular proteases producing bacteria screened with skim milk selective medium and purified with the quadrant streak method. Then, the pure isolate and the resulting extracellular protease were characterized BK1B isolate was a Gram-positive bacterium, while BK1D was a Gram-negative bacterium. BK1B isolate is a halotolerant bacterium and BK1D is a moderate halophilic bacterium. BK1B and BK1D had the largest proteolytic index values, capable of producing extracellular proteases with an optimal production time of three days and pH 6. Extracellular proteases from BK1B and BK1D worked optimally at pH 6 and temperatures of 45 and 42 °C with 2.541 and 3.505 U/mL activity and resistance to NaCl concentrations ranging from 3 - 13% NaCl. This suggests that extracellular proteases from BK1B and BK1D strains can be stable at high salt concentrations.
{"title":"Characterization of extracellular protease from halophilic microbes isolated from Bledug Kuwu, Grobogan, Central Java","authors":"N. Faridah, S. Suharti","doi":"10.1063/5.0052644","DOIUrl":"https://doi.org/10.1063/5.0052644","url":null,"abstract":"High salt-tolerant extracellular protease is an enzyme that plays a crucial role in industries and requires high salt conditions. Some protease enzymes are not resistant to high salt conditions because they may denature under high salt concentrations. Halophilic microbes produce extracellular enzymes that are stable at high salts. This research has succeeded isolating several halophilic microbes capable of producing extracellular proteases from Bledug Kuwu, Grobogan, Central Java, Indonesia. Extracellular proteases producing bacteria screened with skim milk selective medium and purified with the quadrant streak method. Then, the pure isolate and the resulting extracellular protease were characterized BK1B isolate was a Gram-positive bacterium, while BK1D was a Gram-negative bacterium. BK1B isolate is a halotolerant bacterium and BK1D is a moderate halophilic bacterium. BK1B and BK1D had the largest proteolytic index values, capable of producing extracellular proteases with an optimal production time of three days and pH 6. Extracellular proteases from BK1B and BK1D worked optimally at pH 6 and temperatures of 45 and 42 °C with 2.541 and 3.505 U/mL activity and resistance to NaCl concentrations ranging from 3 - 13% NaCl. This suggests that extracellular proteases from BK1B and BK1D strains can be stable at high salt concentrations.","PeriodicalId":365153,"journal":{"name":"INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121968536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sunflower seeds are one of the fourth most widely used sources of edible oil production in the world. The content of unsaturated fatty acids in these oils reaches almost 85%. A previous report showed that a fatty acid can inhibit the growth of bacteria that cause infections which are mostly caused by the nature of bacterial resistance. This study focused on the antibacterial activity of sunflower seed oil derivatives; namely K-salt (K-soap), free fatty acids (FFAs), and fatty acid methyl esters (FAMEs). The fatty acids in sunflower oil is linoleic (49.57%), 11-octadecenoic acid (39.09%), palmitic (9.06%), and stearic (2.28%) acids. The as-synthesized K-soap is solid, white, melting point 167-184 °C, soluble in water and methanol. FFAs are yellow, liquid, boiling point 201 °C, density 0.94 g.cm−3, refractive index 1.46, viscosity 57.30 cSt, soluble in methanol, chloroform, and n-hexane, acid value 171.35, saponification value 176.73, and ester value 5.38. The FAMEs are yellow, liquid, boiling point 185 °C, density 0.89 g.cm−3, refractive index 1.45, viscosity 4.34 cSt, soluble in chloroform and n-hexane. The acid, saponification, and ester values of K-soap, FFAs, and FAMEs are 0.98, 210.96, and 202.98 respectively. They are potential as antibacterial against Escherichia coli and Staphylococcus aureus.
{"title":"Synthesis, characterization, and antibacterial activity of sunflower (Helianthus annus Linn) seed oil derivatives","authors":"S. Sutrisno, Rensa Dwi Assyfah, S. Marfuah","doi":"10.1063/5.0052633","DOIUrl":"https://doi.org/10.1063/5.0052633","url":null,"abstract":"Sunflower seeds are one of the fourth most widely used sources of edible oil production in the world. The content of unsaturated fatty acids in these oils reaches almost 85%. A previous report showed that a fatty acid can inhibit the growth of bacteria that cause infections which are mostly caused by the nature of bacterial resistance. This study focused on the antibacterial activity of sunflower seed oil derivatives; namely K-salt (K-soap), free fatty acids (FFAs), and fatty acid methyl esters (FAMEs). The fatty acids in sunflower oil is linoleic (49.57%), 11-octadecenoic acid (39.09%), palmitic (9.06%), and stearic (2.28%) acids. The as-synthesized K-soap is solid, white, melting point 167-184 °C, soluble in water and methanol. FFAs are yellow, liquid, boiling point 201 °C, density 0.94 g.cm−3, refractive index 1.46, viscosity 57.30 cSt, soluble in methanol, chloroform, and n-hexane, acid value 171.35, saponification value 176.73, and ester value 5.38. The FAMEs are yellow, liquid, boiling point 185 °C, density 0.89 g.cm−3, refractive index 1.45, viscosity 4.34 cSt, soluble in chloroform and n-hexane. The acid, saponification, and ester values of K-soap, FFAs, and FAMEs are 0.98, 210.96, and 202.98 respectively. They are potential as antibacterial against Escherichia coli and Staphylococcus aureus.","PeriodicalId":365153,"journal":{"name":"INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114291364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Lukiati, Sulisetijono, Frida Kunti Setiowati, Anggi Klaritasari
{"title":"Potential prediction of phenolic compounds in red ginger (Zingiber officinale var. rubrum) as an AT1R antagonist by bioinformatics approach for antihypertensive oral drug candidates","authors":"B. Lukiati, Sulisetijono, Frida Kunti Setiowati, Anggi Klaritasari","doi":"10.1063/5.0052726","DOIUrl":"https://doi.org/10.1063/5.0052726","url":null,"abstract":"","PeriodicalId":365153,"journal":{"name":"INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131503974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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