Pub Date : 2019-10-23DOI: 10.5772/intechopen.84709
N. Maddu
{"title":"Functions of Saliva","authors":"N. Maddu","doi":"10.5772/intechopen.84709","DOIUrl":"https://doi.org/10.5772/intechopen.84709","url":null,"abstract":"","PeriodicalId":377273,"journal":{"name":"Saliva and Salivary Diagnostics","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134393900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-23DOI: 10.5772/intechopen.86460
G. Sridharan
{"title":"Introductory Chapter: Saliva - The Future of Disease Diagnostics","authors":"G. Sridharan","doi":"10.5772/intechopen.86460","DOIUrl":"https://doi.org/10.5772/intechopen.86460","url":null,"abstract":"","PeriodicalId":377273,"journal":{"name":"Saliva and Salivary Diagnostics","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116375285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-28DOI: 10.5772/INTECHOPEN.85831
Manohara A. Bhat, Devikripa Bhat
Common oral diseases like dental caries, periodontal diseases and oral cancer have major impact on quality of life. For prevention, treatment and prognosis, it is essential to measure the disease objectively and accurately in a quantitative manner. Quantification of biochemical or molecular specific products of cancers in serum or localized body juices can be one of the current methods of measuring oral diseases objectively. Salivary diagnostics has influenced several researchers and has been verified as an important tool in the diagnosis of many systemic conditions and prognosis of the disease. Developments in the field of molecular biology, salivary genomics and proteomics have directed to the detection of novel molecular markers for oral disease diagnosis, therapeutics and prognosis. DNA methylation profiling promoter
{"title":"Salivary Diagnostics in Oral Diseases","authors":"Manohara A. Bhat, Devikripa Bhat","doi":"10.5772/INTECHOPEN.85831","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.85831","url":null,"abstract":"Common oral diseases like dental caries, periodontal diseases and oral cancer have major impact on quality of life. For prevention, treatment and prognosis, it is essential to measure the disease objectively and accurately in a quantitative manner. Quantification of biochemical or molecular specific products of cancers in serum or localized body juices can be one of the current methods of measuring oral diseases objectively. Salivary diagnostics has influenced several researchers and has been verified as an important tool in the diagnosis of many systemic conditions and prognosis of the disease. Developments in the field of molecular biology, salivary genomics and proteomics have directed to the detection of novel molecular markers for oral disease diagnosis, therapeutics and prognosis. DNA methylation profiling promoter","PeriodicalId":377273,"journal":{"name":"Saliva and Salivary Diagnostics","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116736607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-21DOI: 10.5772/INTECHOPEN.86405
R. Dumache, V. Ciocan, C. Mureşan, Ramona Parvanescu, A. Enache
Since two and a half decades, in human identification, the short tandem repeat (STR) markers represent the “gold standard.” Besides them, haploid markers such as X-STR and Y-STR are also used to complement the autosomal markers. In human identification, DNA from body fluids, especially saliva, represents an important tool. The aim of this chapter is to present the importance of analyz-ing X-STR markers in a relatedness case between a sister and her presumptive brother, a carbonized victim using body fluids for their DNA identification. Our laboratory had to establish the relatedness between a woman and her presumptive brother (PB), who was the victim of a car accident explosion. In this case, as reference sample we used saliva collected on swabs from the woman and blood sample from the deceased victim. For the DNA extraction, DNA IQ Casework (Promega, USA) was used. DNA quantification was done with PowerQuant System kit (Promega, USA). Furthermore, the DNA samples were amplified with Investigator 24plex QS (Qiagen, Germany) for the STR markers and Investigator Argus 12-X QS kit (Qiagen, Germany) for the X-STR markers. The amplified DNA products were separated by capillary electrophoresis on a 3500 Genetic Analyzer. In this case, full genetic profiles were obtained for the woman and her presumptive brother on both STR and X-STR markers. Thus, we could confirm a full sibling relationship between them. Since the introduction of DNA in human identification, it represents a useful tool in establishing sibling relationship from different biological samples.
{"title":"Advantages of Salivary DNA in Human Identification","authors":"R. Dumache, V. Ciocan, C. Mureşan, Ramona Parvanescu, A. Enache","doi":"10.5772/INTECHOPEN.86405","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.86405","url":null,"abstract":"Since two and a half decades, in human identification, the short tandem repeat (STR) markers represent the “gold standard.” Besides them, haploid markers such as X-STR and Y-STR are also used to complement the autosomal markers. In human identification, DNA from body fluids, especially saliva, represents an important tool. The aim of this chapter is to present the importance of analyz-ing X-STR markers in a relatedness case between a sister and her presumptive brother, a carbonized victim using body fluids for their DNA identification. Our laboratory had to establish the relatedness between a woman and her presumptive brother (PB), who was the victim of a car accident explosion. In this case, as reference sample we used saliva collected on swabs from the woman and blood sample from the deceased victim. For the DNA extraction, DNA IQ Casework (Promega, USA) was used. DNA quantification was done with PowerQuant System kit (Promega, USA). Furthermore, the DNA samples were amplified with Investigator 24plex QS (Qiagen, Germany) for the STR markers and Investigator Argus 12-X QS kit (Qiagen, Germany) for the X-STR markers. The amplified DNA products were separated by capillary electrophoresis on a 3500 Genetic Analyzer. In this case, full genetic profiles were obtained for the woman and her presumptive brother on both STR and X-STR markers. Thus, we could confirm a full sibling relationship between them. Since the introduction of DNA in human identification, it represents a useful tool in establishing sibling relationship from different biological samples.","PeriodicalId":377273,"journal":{"name":"Saliva and Salivary Diagnostics","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125079088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-04DOI: 10.5772/INTECHOPEN.85762
C. Streckfus
Technological developments are propelling medical diagnostics forward at unprecedented rates. Advancements in genomic, proteomics and cellomics are leading the way for diagnostic tests that will be capable of rapid multi-analyte detection in both laboratory and non-laboratory settings. Currently, proof-of-principle has been demonstrated for salivary biomarkers, whose up and down regulation has been shown to correlate strongly with breast cancer among women. Consequently, the rationale for this chapter is to review the medical literature and present the current research focused on the use of saliva as a diagnostic medium for the study of early breast cancer progression.
{"title":"Salivary Biomarkers to Assess Breast Cancer Diagnosis and Progression: Are We There Yet?","authors":"C. Streckfus","doi":"10.5772/INTECHOPEN.85762","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.85762","url":null,"abstract":"Technological developments are propelling medical diagnostics forward at unprecedented rates. Advancements in genomic, proteomics and cellomics are leading the way for diagnostic tests that will be capable of rapid multi-analyte detection in both laboratory and non-laboratory settings. Currently, proof-of-principle has been demonstrated for salivary biomarkers, whose up and down regulation has been shown to correlate strongly with breast cancer among women. Consequently, the rationale for this chapter is to review the medical literature and present the current research focused on the use of saliva as a diagnostic medium for the study of early breast cancer progression.","PeriodicalId":377273,"journal":{"name":"Saliva and Salivary Diagnostics","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125588120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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