The present study was conducted to determine the influence of Cr on some agronomic characters of Telfairia occidentalis nutrient content and uptake and some chemical properties of soil. In the greenhouse trial, chromium nitrate [Cr(N0 3 ) 2 ] was applied at rates of 0, 50, 100 and 200 mg per 5 kg sieved and air-dried soil obtained from a depth of 0-15cm. The rates of 0, 20, 40 and 80 kgha equivalent to pot rates were used in the field trial. Results showed that the soil used was texturally sandy loam and an ultisol as revealed by its low base saturation. In the greenhouse the soil pH, N, K, Mg, Ca, Na, Fe, Mn, Zn, free Fe and Al oxides, organic carbon, effective cation exchange capacity, exchangeable acidity, amorphous Fe and Al oxides content of the soil decreased inconsistently at various levels of Cr treatments except available P, which appreciated inconsistently. With the exception of soil pH, organic carbon, available P and amorphous Fe oxide, which increased at various levels of Cr concentrations, all other soil chemical properties determined, declined inconsistently in the field trial. The amorphous Al oxide however remained stable in the field trial. The Cr content of the soil increased with the levels of Cr treatments when compared with the control in the trials. The N, P, K, Mg, Ca, Na, Fe, Mn and Zn content of shoot and root as well as their uptake also decreased consistently with increasing Cr treatments. In addition, the Cr content as well as uptake by the shoot and root also increased consistently with increased rates of the Cr applied in the trials with the minimum levels of the Cr content and uptake recorded at the control treatments. As the Cr concentration increased, the crude protein content of both shoot and root consistently decreased with highest crude protein content recorded in the shoot compared to the root. A decrease in the dry matter yield with increased Cr treatments in shoot and root was recorded in the trials. Results also showed that the Cr influenced the height, collar girth, leaf area and number of leaves with control treatments higher than other treatments at final harvest.
{"title":"Phyto-availability of chromium to fluted pumpkin (Telfairi occidentalis Hook F) in an ultisol.","authors":"E. Orhue, U. Frank","doi":"10.31018/jans.v3i2.178","DOIUrl":"https://doi.org/10.31018/jans.v3i2.178","url":null,"abstract":"The present study was conducted to determine the influence of Cr on some agronomic characters of Telfairia occidentalis nutrient content and uptake and some chemical properties of soil. In the greenhouse trial, chromium nitrate [Cr(N0 3 ) 2 ] was applied at rates of 0, 50, 100 and 200 mg per 5 kg sieved and air-dried soil obtained from a depth of 0-15cm. The rates of 0, 20, 40 and 80 kgha equivalent to pot rates were used in the field trial. Results showed that the soil used was texturally sandy loam and an ultisol as revealed by its low base saturation. In the greenhouse the soil pH, N, K, Mg, Ca, Na, Fe, Mn, Zn, free Fe and Al oxides, organic carbon, effective cation exchange capacity, exchangeable acidity, amorphous Fe and Al oxides content of the soil decreased inconsistently at various levels of Cr treatments except available P, which appreciated inconsistently. With the exception of soil pH, organic carbon, available P and amorphous Fe oxide, which increased at various levels of Cr concentrations, all other soil chemical properties determined, declined inconsistently in the field trial. The amorphous Al oxide however remained stable in the field trial. The Cr content of the soil increased with the levels of Cr treatments when compared with the control in the trials. The N, P, K, Mg, Ca, Na, Fe, Mn and Zn content of shoot and root as well as their uptake also decreased consistently with increasing Cr treatments. In addition, the Cr content as well as uptake by the shoot and root also increased consistently with increased rates of the Cr applied in the trials with the minimum levels of the Cr content and uptake recorded at the control treatments. As the Cr concentration increased, the crude protein content of both shoot and root consistently decreased with highest crude protein content recorded in the shoot compared to the root. A decrease in the dry matter yield with increased Cr treatments in shoot and root was recorded in the trials. Results also showed that the Cr influenced the height, collar girth, leaf area and number of leaves with control treatments higher than other treatments at final harvest.","PeriodicalId":413604,"journal":{"name":"Research Journal of Agriculture and Biological Sciences","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128097995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. A. Ismail, O. El-sayed, S. Helmy, Y. Mohammad, N. Mohammad
Cyclodextrin glucanotransferase- (CGTase), (EC.2.4.1.19) producing bacteria were isolated from different sources of soils and identified as Bacillus cereus N1 and the best source was that obtained from National Research Centre. The maximum production of the crude CGTase enzyme was observed after 48h of incubation at 37 o C producing CGTase activity of 3.5 U/ml. The effect of nutritional requirements on the CGTase production was carried out. Soluble starch and yeast extracts were found to be the best carbon and nitrogen sources, respectively. The enzyme was successively purified by ammonium sulphate precipitation, DEAE-cellulose, and sephadex G-100 column chromatography, and the final specific activity of CGTase enzyme was increased by 24 fold. The SDS-PAGE showed that the purified CGTase enzyme was homogenous and the molecular weight of the purified enzyme was about 75 kDa. The characterization of the enzyme exhibited optimum pH and temperature at 6.0 and 40 ° C, respectively. The enzyme was stable at pH 6.5 to 8.0 and retained its high activity up to 45 ° C.
{"title":"Production, Purification and Biochemical Characteriztion of Cyclodextrin Glucanotransferase from Bacillus Cereus N1","authors":"S. A. Ismail, O. El-sayed, S. Helmy, Y. Mohammad, N. Mohammad","doi":"10.21608/ejm.2009.287","DOIUrl":"https://doi.org/10.21608/ejm.2009.287","url":null,"abstract":"Cyclodextrin glucanotransferase- (CGTase), (EC.2.4.1.19) producing bacteria were isolated from different sources of soils and identified as Bacillus cereus N1 and the best source was that obtained from National Research Centre. The maximum production of the crude CGTase enzyme was observed after 48h of incubation at 37 o C producing CGTase activity of 3.5 U/ml. The effect of nutritional requirements on the CGTase production was carried out. Soluble starch and yeast extracts were found to be the best carbon and nitrogen sources, respectively. The enzyme was successively purified by ammonium sulphate precipitation, DEAE-cellulose, and sephadex G-100 column chromatography, and the final specific activity of CGTase enzyme was increased by 24 fold. The SDS-PAGE showed that the purified CGTase enzyme was homogenous and the molecular weight of the purified enzyme was about 75 kDa. The characterization of the enzyme exhibited optimum pH and temperature at 6.0 and 40 ° C, respectively. The enzyme was stable at pH 6.5 to 8.0 and retained its high activity up to 45 ° C.","PeriodicalId":413604,"journal":{"name":"Research Journal of Agriculture and Biological Sciences","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125656038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.22587/rjabs.2019.14.1.2
{"title":"Modern Developments of Polymer Blend/ Oxide\u0000Nanocomposites for Biomedical Applications as\u0000Antibacterial and Radiation Shielding Materials: A Review","authors":"","doi":"10.22587/rjabs.2019.14.1.2","DOIUrl":"https://doi.org/10.22587/rjabs.2019.14.1.2","url":null,"abstract":"","PeriodicalId":413604,"journal":{"name":"Research Journal of Agriculture and Biological Sciences","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132209395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.22587/rjabs.2019.14.1.1
{"title":"The Role of Vermicompost and Foliar Spray of Spirulina\u0000Platensis Extract on Vegetative Growth, Yield and Nutrition\u0000Status of Lettuce Plant under sandy soil","authors":"","doi":"10.22587/rjabs.2019.14.1.1","DOIUrl":"https://doi.org/10.22587/rjabs.2019.14.1.1","url":null,"abstract":"","PeriodicalId":413604,"journal":{"name":"Research Journal of Agriculture and Biological Sciences","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130248429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.22587/rjabs.2019.14.3.1
{"title":"Study of Replacement of Compost with Vermicompost and\u0000Its Effect on Quality and Quantity Yield and Nutritional\u0000Status of Sweet Fennel Plants Grown in Sandy Soil","authors":"","doi":"10.22587/rjabs.2019.14.3.1","DOIUrl":"https://doi.org/10.22587/rjabs.2019.14.3.1","url":null,"abstract":"","PeriodicalId":413604,"journal":{"name":"Research Journal of Agriculture and Biological Sciences","volume":"2508 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131299293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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