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Practical Implications of Different Phenotypic and Molecular Responses of Evergreen Conifer and Broadleaf Deciduous Forest Tree Species to Regulated Water Deficit in a Container Nursery 常绿针叶林和阔叶落叶树种对容器苗圃水分亏缺调节的不同表型和分子响应的实际意义
Pub Date : 2020-08-09 DOI: 10.20944/preprints202008.0224.v1
P. Robakowski, T. Wyka, W. Kowalkowski, W. Barzdajn, E. Pers-kamczyc, Artur Jankowski, B. Politycka
Recent climatic changes have resulted in an increased frequency and prolonged periods of drought and strained water resources affecting plant production. We explored the possibility of reducing irrigation in a container nursery and studied the growth responses of seedlings of four economically important forest trees: broadleaf deciduous angiosperms Fagus sylvatica L., Quercus petraea (Matt.) Liebl., and evergreen conifers Abies alba Mill. and Pinus sylvestris L. We also studied markers of water stress including modifications of biomass allocation, leaf anatomy, proline accumulation, and expression of selected genes. Growth of the broadleaved deciduous species was more sensitive to the reduced water supply than that of conifers. Remarkably, growth of the shade tolerant Abies was not affected. Adjustment of biomass allocations was strongest in P. sylvestris, with a remarkable increase in allocation to roots. In response to water deficit both deciduous species accumulated proline in leaves and produced leaves with shorter palisade cells, reduced vascular tissues, and smaller conduit diameters. These responses did not occur in conifers. Relative transcript abundance of a gene encoding the Zn-finger protein in Q. petraea and a gene encoding the pore calcium channel protein 1 in A. alba increased as water deficit increased. Our study shows major differences between functional groups in response to irrigation, with seedlings of evergreen conifers having higher tolerance than the deciduous species. This suggests that major water savings could be achieved by adjusting irrigation regime to functional group or species requirements.
最近的气候变化导致干旱的频率增加和时间延长,水资源紧张影响到植物生产。探讨了在集装箱苗圃中减少灌溉的可能性,研究了阔叶落叶被子植物Fagus sylvatica L.、栎(Quercus petaea)等4种重要经济树种幼苗的生长响应。Liebl。和常绿针叶树冷杉。我们还研究了水分胁迫的标志,包括生物量分配、叶片解剖、脯氨酸积累和选定基因表达的改变。阔叶落叶树种的生长对供水减少的敏感性高于针叶树。值得注意的是,耐阴冷杉的生长不受影响。生物量分配的调整以柽柳生物量分配的调整最为明显,对根系的分配显著增加。在水分亏缺的条件下,两种落叶植物叶片中脯氨酸积累较多,叶片栅栏细胞变短,维管组织减少,导管直径变小。这些反应在针叶树中没有发生。随着水分亏缺的增加,黄豆中编码锌指蛋白的基因和白豆中编码孔钙通道蛋白1的基因的相对转录丰度增加。结果表明,不同功能群对灌溉的响应存在较大差异,常绿针叶树幼苗的耐受性高于落叶树种。这表明,通过调整灌溉制度以适应功能群或物种的需求,可以实现重大的节水。
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引用次数: 9
The Profitability of Cross-Cutting Practices in Butt-Rotten Picea abies Final-Felling Stands 枯死云杉最终采伐林的交叉采伐效益研究
Pub Date : 2019-10-05 DOI: 10.3390/f10100874
K. Kärhä, M. Räsänen, T. Palander
Research Highlights: This study offers new information on the cross cutting of decayed stems with the sounding of short (0.5 m) offcuts and the bucking of longer (3.0 m) butt-rotten poles. Background and Objectives: The root and butt-rot fungus Heterobasidion annosum sensu lato (Fr.) Bref. causes wood quality damage to trees in softwood forests. When timber is harvested in butt-rotten forests, it is essential that the decayed part of the tree is recognized and cut away from a stem, while the healthy and good quality log section of a stem is cross cut with precision sawlogs. The objective of the study was to investigate the impact of two off-cutting methods on stem processing time, cutting productivity, sawlog volume, and commercial value at the roadside landing when harvesting timber from the butt-rotten Norway spruce (Picea abies (L.) Karst.) final-felling forests. Materials and Methods: The length of the short offcuts used was 0.5 m. The results of the cross-cutting practices were compared to the decayed pulpwood poles of 3 m from the butt of the rotten stems. Time and motion studies were carried out in stands before the profitability calculations. The study data consisted of 1980 Norway spruce sawlog stems. Results: Sounding of the short offcuts added significantly to the stem processing time of butt-rotten stems, but the sawlog volume and the timber value recovery of the stems were higher than those of the decayed pulpwood poles of 3 m. Conclusions: The study concluded that sounding of butt-rotten Norway spruce stems with one to three offcuts is economically profitable if the diameter of the decayed column at the stem stump’s height is small (≤5 cm). In contrast, when the width of the decay is larger (>5 cm), it is more profitable to first cross cut the decayed pulpwood pole of 3 m and then to observe the height of the decayed part of the stem.
研究亮点:本研究提供了关于腐烂茎的交叉切割的新信息,其中短(0.5 m)的侧边发出声音,长(3.0 m)的腐杆弯曲。背景与目的:根腐菌异霉(Heterobasidion annosum sensu lato, Fr.) Bref。对针叶林的树木造成木材质量损害。在枯木森林中采伐木材时,必须识别树木的腐烂部分并将其从茎上切下,而茎上健康且质量良好的原木部分则用精确的锯木进行横切。本研究的目的是调查两种断伐方法在采伐挪威云杉(Picea abies (L.))木材时对茎加工时间、砍伐生产率、锯木量和路边降落时的商业价值的影响。喀斯特)最终采伐的森林。材料与方法:所用短切边长度为0.5 m。将交叉切割的结果与距腐茎尾部3 m处的腐烂纸浆木杆进行了比较。在盈利能力计算之前,在展台上进行了时间和运动研究。研究数据包括1980年挪威云杉锯木茎。结果:短边段测深显著延长了对角腐茎的加工时间,但树干的锯材体积和木材价值回收率高于3 m腐烂的纸浆木杆。结论:研究认为,如果挪威云杉残茎高度处的腐柱直径较小(≤5 cm),对残茎进行1 ~ 3个截枝的探深具有经济效益。相比之下,当腐烂宽度较大(>5 cm)时,先横切3 m的腐烂纸浆木杆,然后观察树干腐烂部分的高度更为有利。
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引用次数: 4
Understanding Land–Atmosphere–Climate Coupling from the Canadian Prairie Dataset 从加拿大草原数据集理解陆地-大气-气候耦合
Pub Date : 2018-11-08 DOI: 10.3390/ENVIRONMENTS5120129
A. Betts, R. Desjardins
Analysis of the hourly Canadian Prairie data for the past 60 years has transformed our quantitative understanding of land–atmosphere–cloud coupling. The key reason is that trained observers made hourly estimates of the opaque cloud fraction that obscures the sun, moon, or stars, following the same protocol for 60 years at all stations. These 24 daily estimates of opaque cloud data are of sufficient quality such that they can be calibrated against Baseline Surface Radiation Network data to yield the climatology of the daily short-wave, long-wave, and total cloud forcing (SWCF, LWCF and CF, respectively). This key radiative forcing has not been available previously for climate datasets. Net cloud radiative forcing changes sign from negative in the warm season, to positive in the cold season, when reflective snow reduces the negative SWCF below the positive LWCF. This in turn leads to a large climate discontinuity with snow cover, with a systematic cooling of 10 °C or more with snow cover. In addition, snow cover transforms the coupling between cloud cover and the diurnal range of temperature. In the warm season, maximum temperature increases with decreasing cloud, while minimum temperature barely changes; while in the cold season with snow cover, maximum temperature decreases with decreasing cloud, and minimum temperature decreases even more. In the warm season, the diurnal ranges of temperature, relative humidity, equivalent potential temperature, and the pressure height of the lifting condensation level are all tightly coupled to the opaque cloud cover. Given over 600 station-years of hourly data, we are able to extract, perhaps for the first time, the coupling between the cloud forcing and the warm season imbalance of the diurnal cycle, which changes monotonically from a warming and drying under clear skies to a cooling and moistening under cloudy skies with precipitation. Because we have the daily cloud radiative forcing, which is large, we are able to show that the memory of water storage anomalies, from precipitation and the snowpack, goes back many months. The spring climatology shows the memory of snowfall back through the entire winter, and the memory in summer, goes back to the months of snowmelt. Lagged precipitation anomalies modify the thermodynamic coupling of the diurnal cycle to the cloud forcing, and shift the diurnal cycle of the mixing ratio, which has a double peak. The seasonal extraction of the surface total water storage is a large damping of the interannual variability of precipitation anomalies in the growing season. The large land-use change from summer fallow to intensive cropping, which peaked in the early 1990s, has led to a coupled climate response that has cooled and moistened the growing season, lowering cloud-base, increasing equivalent potential temperature, and increasing precipitation. We show a simplified energy balance of the Prairies during the growing season, and its dependence on reflective cloud.
对过去60年每小时加拿大草原数据的分析改变了我们对陆地-大气-云耦合的定量理解。关键原因是训练有素的观测者每小时对遮挡太阳、月亮或星星的不透明云层进行估计,所有观测站60年来都遵循相同的程序。这24个不透明云数据的每日估计具有足够的质量,因此它们可以根据基线地面辐射网数据进行校准,从而产生每日短波、长波和总云强迫(分别为SWCF、LWCF和CF)的气候学。这一关键的辐射强迫在以前的气候数据集中是不可用的。净云辐射强迫从暖季的负值变为冷季的正值,此时反射雪使负SWCF低于正LWCF。这反过来又导致有积雪的大的气候不连续,有积雪的情况下系统地降温10°C或更高。此外,积雪改变了云量与温度日变化之间的耦合关系。暖季最高气温随云量减少而升高,最低气温变化不大;而在冰雪覆盖的寒冷季节,最高气温随着云量的减少而下降,最低气温下降幅度更大。在暖季,气温、相对湿度、等效位温的日变化范围以及上升凝结层的压力高度都与不透明云层紧密耦合。基于600多个台站年的每小时数据,我们能够(也许是第一次)提取出云强迫与暖季日循环不平衡之间的耦合,暖季日循环从晴朗天气下的变暖干燥到多云天气下有降水的变冷湿润单调变化。因为我们有每天的云辐射强迫,这是很大的,我们能够显示出水储存异常的记忆,从降水和积雪,可以追溯到好几个月前。春季气候学显示了整个冬季的降雪记忆,而夏季的记忆则追溯到融雪的月份。滞后降水异常改变了日循环与云强迫的热力耦合,改变了混合比的日循环,混合比具有双峰特征。地表总蓄水量的季节性提取对生长季节降水异常的年际变化有很大的抑制作用。从夏季休耕到集约种植的大规模土地利用变化在20世纪90年代初达到顶峰,导致了生长季节降温和湿润、云基降低、等效潜在温度升高和降水增加的耦合气候响应。我们展示了一个简化的草原在生长季节的能量平衡,以及它对反射云的依赖。
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引用次数: 2
Time Series Analysis of MODIS Derived NDVI for the Hluhluwe-Imfolozi Park, South Africa: Impact of Recent Intense Drought 南非Hluhluwe-Imfolozi公园MODIS NDVI时间序列分析:近期强烈干旱的影响
Pub Date : 2018-09-26 DOI: 10.3390/CLI6040095
N. Mbatha, Sifiso Xulu
The variability of temperature and precipitation influenced by El Niño-Southern Oscillation (ENSO) is potentially one of key factors contributing to vegetation product in southern Africa. Thus, understanding large-scale ocean–atmospheric phenomena like the ENSO and Indian Ocean Dipole/Dipole Mode Index (DMI) is important. In this study, 16 years (2002–2017) of Moderate Resolution Imaging Spectroradiometer (MODIS) Terra/Aqua 16-day normalized difference vegetation index (NDVI), extracted and processed using JavaScript code editor in the Google Earth Engine (GEE) platform was used to analyze the vegetation response pattern of the oldest proclaimed nature reserve in Africa, the Hluhluwe-iMfolozi Park (HiP) to climatic variability. The MODIS enhanced vegetation index (EVI), burned area index (BAI), and normalized difference infrared index (NDII) were also analyzed. The study used the Modern Retrospective Analysis for the Research Application (MERRA) model monthly mean soil temperature and precipitations. The Global Land Data Assimilation System (GLDAS) evapotranspiration (ET) data were used to investigate the HiP vegetation water stress. The region in the southern part of the HiP which has land cover dominated by savanna experienced the most impact of the strong El Niño. Both the HiP NDVI inter-annual Mann–Kendal trend test and sequential Mann–Kendall (SQ-MK) test indicated a significant downward trend during the El Niño years of 2003 and 2014–2015. The SQ-MK significant trend turning point which was thought to be associated with the 2014–2015 El Niño periods begun in November 2012. The wavelet coherence and coherence phase indicated a positive teleconnection/correlation between soil temperatures, precipitation, soil moisture (NDII), and ET. This was explained by a dominant in-phase relationship between the NDVI and climatic parameters especially at a period band of 8–16 months.
受El Niño-Southern涛动(ENSO)影响的温度和降水变率可能是影响南部非洲植被产品的关键因素之一。因此,了解像ENSO和印度洋偶极子/偶极子模式指数(DMI)这样的大规模海洋-大气现象是很重要的。本研究利用Google Earth Engine (GEE)平台上的JavaScript代码编辑器提取和处理的16年(2002-2017)MODIS Terra/Aqua 16天归一化植被指数(NDVI),分析了非洲最古老自然保护区Hluhluwe-iMfolozi Park (HiP)植被对气候变率的响应模式。对MODIS增强植被指数(EVI)、烧伤面积指数(BAI)和归一化红外差指数(NDII)进行了分析。该研究使用了研究应用的现代回顾性分析(MERRA)模式,月平均土壤温度和降水。利用全球土地资料同化系统(GLDAS)蒸散发(ET)数据研究了青藏高原植被水分胁迫。青藏高原南部以热带稀树草原为主的地区受强厄尔尼诺Niño的影响最大。HiP NDVI年际Mann-Kendal趋势检验和序列Mann-Kendall (SQ-MK)检验均显示2003年和2014-2015年El Niño年呈显著下降趋势。SQ-MK显著趋势转折点被认为与2012年11月开始的2014-2015年El Niño时期有关。小波相干性和相干相位表明,土壤温度、降水、土壤湿度(NDII)和ET之间存在正的遥相关关系,这可以解释为NDVI与气候参数之间存在主要的相位关系,特别是在8-16个月的周期内。
{"title":"Time Series Analysis of MODIS Derived NDVI for the Hluhluwe-Imfolozi Park, South Africa: Impact of Recent Intense Drought","authors":"N. Mbatha, Sifiso Xulu","doi":"10.3390/CLI6040095","DOIUrl":"https://doi.org/10.3390/CLI6040095","url":null,"abstract":"The variability of temperature and precipitation influenced by El Niño-Southern Oscillation (ENSO) is potentially one of key factors contributing to vegetation product in southern Africa. Thus, understanding large-scale ocean–atmospheric phenomena like the ENSO and Indian Ocean Dipole/Dipole Mode Index (DMI) is important. In this study, 16 years (2002–2017) of Moderate Resolution Imaging Spectroradiometer (MODIS) Terra/Aqua 16-day normalized difference vegetation index (NDVI), extracted and processed using JavaScript code editor in the Google Earth Engine (GEE) platform was used to analyze the vegetation response pattern of the oldest proclaimed nature reserve in Africa, the Hluhluwe-iMfolozi Park (HiP) to climatic variability. The MODIS enhanced vegetation index (EVI), burned area index (BAI), and normalized difference infrared index (NDII) were also analyzed. The study used the Modern Retrospective Analysis for the Research Application (MERRA) model monthly mean soil temperature and precipitations. The Global Land Data Assimilation System (GLDAS) evapotranspiration (ET) data were used to investigate the HiP vegetation water stress. The region in the southern part of the HiP which has land cover dominated by savanna experienced the most impact of the strong El Niño. Both the HiP NDVI inter-annual Mann–Kendal trend test and sequential Mann–Kendall (SQ-MK) test indicated a significant downward trend during the El Niño years of 2003 and 2014–2015. The SQ-MK significant trend turning point which was thought to be associated with the 2014–2015 El Niño periods begun in November 2012. The wavelet coherence and coherence phase indicated a positive teleconnection/correlation between soil temperatures, precipitation, soil moisture (NDII), and ET. This was explained by a dominant in-phase relationship between the NDVI and climatic parameters especially at a period band of 8–16 months.","PeriodicalId":426802,"journal":{"name":"Prime Archives in Environmental Research","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129076418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 36
Atmospheric Aerosol Over Ukraine Region: Current Status of Knowledge and Research Efforts 乌克兰地区大气气溶胶:知识现状和研究成果
Pub Date : 2018-06-25 DOI: 10.3389/fenvs.2018.00059
G. Milinevsky, V. Danylevsky
In this paper the current status of knowledge and research efforts on atmospheric aerosol investigation over Ukraine region are reviewed. Several earlier results of atmospheric extinction, aerosol content and properties studies performed in Ukraine during the second part of 20 century are discussed. The recent findings on aerosol optical depth, Angstrom exponent, optical and microphysical properties of aerosol particles (single-scattering albedo, size distribution, complex refractive index) and their seasonal variability obtained from both AERONET and portable sun-photometers measurements during the 2008–2016 period are presented and analyzed. Data of POLDER/PARASOL satellite instrument were also involved to study the aerosol properties over Ukraine and neighbor countries. The results showed that aerosol content and properties over Ukraine are very similar to ones over rest European urban regions but considerably lower than over polluted China territories. The first lidar measurements and the air quality evaluations by the PM concentration measurements in Ukraine are also discussed. The aerosol sources in Ukraine and surrounding territories are considered from analysis of the air mass back trajectory and simulation by GEOS-Chem model. The future satellite project Aerosol-UA for global aerosol studies by measurements of the scattered solar radiation polarization is discussed in the article.
本文综述了乌克兰地区大气气溶胶调查的知识现状和研究工作。讨论了20世纪下半叶在乌克兰进行的大气消失、气溶胶含量和特性研究的几个早期结果。本文介绍并分析了2008-2016年期间AERONET和便携式太阳光度计测量的气溶胶光学深度、埃指数、气溶胶粒子的光学和微物理性质(单散射反照率、尺寸分布、复折射率)及其季节变化的最新发现。还利用POLDER/PARASOL卫星仪器的数据对乌克兰及其邻国的气溶胶特性进行了研究。结果表明,乌克兰上空的气溶胶含量和性质与欧洲其他城市地区非常相似,但远低于中国污染地区。本文还讨论了乌克兰首次激光雷达测量和PM浓度测量对空气质量的评价。通过对气团回返轨迹的分析和GEOS-Chem模型的模拟,考虑了乌克兰及周边地区的气溶胶源。本文讨论了利用散射太阳辐射偏振测量全球气溶胶研究的未来卫星项目气溶胶- ua。
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引用次数: 11
From Litter to Humus in a Norwegian Spruce Forest Long-Term Studies on the Decomposition of Needles and Cones 挪威云杉林从凋落物到腐殖质针叶和球果分解的长期研究
Pub Date : 2016-08-25 DOI: 10.3390/F7090186
S. Hågvar
The aim of this review and synthesis is to illustrate the gradual transformation of needles and cones from litter to humus. Individual needles may follow quite different decomposition pathways, which contributes to a diverse humus structure. In the litter layer (Oi), about 40% of the needles were excavated by special mites that produced slowly decomposable excrements. In the fermentation layer (Oe), needles which happened to be in close contact with fine roots decomposed more rapidly. Cones decomposed slower than needles during the first 3–5 years, so the role of cones in carbon (C) storage may be greater than indicated by their fraction of fresh litter. Over a 13 years period, potassium (K), magnesium (Mg) and phosphorus (P) in cones was released, while the total amount of calcium (Ca), manganese (Mn), iron (Fe) and aluminium (Al) increased strongly. Nitrogen concentration increased but the total nitrogen content remained rather constant. After 13 years, the cones had sunk about 6 cm into the soil and lost 60% of their dry weight but were morphologically intact. A cone monitored for 28 years was fully recognizable and had not yet reached the stable Oa layer. The most inert decomposition products in the Oa layer were fragments of needles and cone scales, microarthropod excrements and chitinous insect remains.
本文综述和综合的目的是阐明针叶和球果从凋落物到腐殖质的逐渐转变。单个针可能遵循完全不同的分解途径,这有助于腐殖质结构的多样性。在凋落物层(Oi)中,大约40%的针被特殊的螨虫挖出,产生缓慢分解的排泄物。在发酵层(Oe)中,与细根接触较近的针叶分解速度较快。在前3 ~ 5年,球果的分解速度比针叶慢,因此球果在碳(C)储存中的作用可能大于其在新鲜凋落物中所占的比例。在13年的时间里,球果中钾(K)、镁(Mg)和磷(P)的释放量显著增加,钙(Ca)、锰(Mn)、铁(Fe)和铝(Al)的总量显著增加。氮浓度增加,但总氮含量基本保持不变。13年后,球果沉入土壤约6厘米,失去了60%的干重,但形态完好无损。一个监测了28年的圆锥体是完全可识别的,尚未达到稳定的Oa层。Oa层的惰性分解产物主要是针叶和锥体鳞片碎片、小节肢动物排泄物和几丁质昆虫残骸。
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引用次数: 9
Oil and Gas Production Wastewater: Soil Contamination and Pollution Prevention 油气生产废水:土壤污染与污染防治
Pub Date : 2016-03-20 DOI: 10.1155/2016/2707989
J. Pichtel
During oil and natural gas production, so-called “produced water” comprises the largest byproduct stream. In addition, many oil and gas operations are augmented via injection of hydraulic fracturing (HF) fluids into the formation. Both produced water and HF fluids may contain hundreds of individual chemicals, some known to be detrimental to public health and the environment. Oil and gas production wastewater may serve a range of beneficial purposes, particularly in arid regions, if managed correctly. Numerous treatment technologies have been developed that allow for injection, discharge to the land surface, or beneficial reuse. Although many papers have addressed the effects of oil and gas production wastewater (OGPW) on groundwater and surface water quality, significantly less information is available on the effects of these fluids on the soil resource. This review paper compiles fundamental information on numerous chemicals used and produced during oil and gas development and their effects on the soil environment. Additionally, pollution prevention technologies relating to OGPW are presented. An understanding of the effects of OGPW on soil chemical, physical, and biological properties can provide a foundation for effective remediation of OGPW-affected soils; additionally, sustainable reuse of oil and gas water for irrigation and industrial purposes may be enhanced.
在石油和天然气生产过程中,所谓的“采出水”是最大的副产品流。此外,许多油气作业都是通过向地层注入水力压裂液(HF)来实现的。采出水和HF流体都可能含有数百种单独的化学物质,其中一些已知对公众健康和环境有害。如果管理得当,油气生产废水可以用于一系列有益的目的,特别是在干旱地区。已经开发了许多处理技术,允许注入,排放到陆地表面,或有益的再利用。尽管许多论文都讨论了油气生产废水(OGPW)对地下水和地表水质量的影响,但关于这些流体对土壤资源的影响的信息却少得多。这篇综述汇编了石油和天然气开发过程中使用和产生的许多化学品及其对土壤环境的影响的基本资料。此外,还介绍了与OGPW有关的污染防治技术。了解OGPW对土壤化学、物理和生物特性的影响可以为有效修复受OGPW影响的土壤提供基础;此外,可以加强用于灌溉和工业目的的石油和天然气水的可持续再利用。
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引用次数: 129
Near-surface biases in ERA5 over the Canadian Prairies ERA5在加拿大大草原上的近地表偏差
Pub Date : 1900-01-01 DOI: 10.37247/paenvr.1.2020.8
Alan K. Betts, Darren Z Chan, Raymond L Desjardins
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引用次数: 15
Assessing In Vitro Biofilm-Forming Ability and Susceptibility to Food-Grade Sanitizers of Listeria Monocytogenes Isolates from a Delicatessen Food Industry 熟食行业单核细胞增生李斯特菌体外生物膜形成能力及对食品级消毒剂敏感性的评估
Pub Date : 1900-01-01 DOI: 10.37247/paenvr.1.2021.38
Joana Catarina Andrade, António Lopes João, Carlos de Sousa Alonso, A. Barreto, A. Henriques
Assessing In Vitro Biofilm-Forming Ability and Susceptibility to Food-Grade Sanitizers of Listeria Monocytogenes Isolates from a Delicatessen Food Abstract Listeria monocytogenes is a human pathogen commonly found in food-related environments. L. monocytogenes is believed to occur in food contact surfaces in biofilms, exhibiting less susceptibility to sanitizers than their planktonic form. Food-grade sanitizers are widely used in food processing plants for contamination control purposes. The aim of this work was to investigate the susceptibility of L. monocytogenes to commercial food-grade sanitizers and to benzalkonium chloride; isolates were tested in the planktonic form and in biofilms and were further assessed using genetic subtyping. Raw materials, final products and food-contact surfaces of a delicatessen food industry were tested during a 6-year period for L. monocytogenes. confirmation and serogrouping was performed by polymerase chain reaction (PCR), revealing serogoup IIc as the most common (53%). Genetic variability of the isolates was evidenced using Pulsed-field gel electrophoresis (PFGE) and three clusters were obtained. In the biofilm-forming ability assay, isolates were classified as weak biofilm producers. L. monocytogenes strains were susceptible both in the planktonic and biofilm form to oxidizing and ethanol-based compounds and to benzalkonium chloride, but not to quaternary ammonium compound. A positive association of the biofilm-forming ability and LD 90 values was found for quaternary ammonium compound and benzalkonium chloride. Overall, these results confirm the need for conscious selection and use of sanitizers in food-related environments for Listeria monocytogenes improved control .
摘要单核细胞增生李斯特菌是一种常见于食品相关环境中的人类病原体。单核细胞增生乳杆菌被认为发生在生物膜的食物接触表面,与浮游形式相比,对消毒剂的敏感性较低。食品级杀菌剂广泛用于食品加工厂的污染控制。本研究的目的是研究单核增生乳杆菌对商用食品级消毒剂和苯扎氯铵的敏感性;分离物以浮游形式和生物膜形式进行了测试,并使用遗传分型进一步进行了评估。在6年的时间里,对一家熟食行业的原材料、最终产品和食品接触表面进行了单核细胞增生乳杆菌的检测。采用聚合酶链反应(PCR)进行确诊和血清分型,显示IIc型为最常见(53%)。利用脉冲场凝胶电泳(PFGE)证实了分离物的遗传变异,并获得了三个簇。在生物膜形成能力测试中,分离株被归类为弱生物膜生产者。浮游和生物膜形态的单核增生乳杆菌对氧化性、乙醇性化合物和苯扎氯铵敏感,对季铵性化合物不敏感。季铵化合物和苯扎氯铵的生物膜形成能力与ld90值呈正相关。总的来说,这些结果证实了在食品相关环境中有意识地选择和使用消毒剂的必要性,以改善单核细胞增生李斯特菌的控制。
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引用次数: 0
Information is Selection-A Review of Basics Shows Substantial Potential for Improvement of Digital Information Representation 信息是选择——对基础知识的回顾显示了数字信息表示改进的巨大潜力
Pub Date : 1900-01-01 DOI: 10.37247/paenvr.1.2020.20
W. Orthuber
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引用次数: 1
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