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Current Advances in Chemistry and Biochemistry Vol. 3最新文献

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Study on Proteomic Profiling of High-grade Glioblastoma: Aspects of Biomarkers 高级别胶质母细胞瘤的蛋白质组学分析:生物标志物方面的研究
Pub Date : 2021-03-04 DOI: 10.9734/BPI/CACB/V3/1688F
S. Naryzhny, M. Maynskova, V. Zgoda, N. Ronzhina, S. Novikova, N. Belyakova, Olga A. Kleyst, O. Legina, R. Pantina, M. V. Filatov
Identification and quantitative analysis of different proteoforms (protein species) presented in a cell line generated from high grade glioblastoma was performed using two-dimensional electrophoresis (2DE), mass spectrometry (ESI LC-MS/MS), and immunodetection. A 2DE protein map containing an extensive data set comprising 937 spots with 1542 unique protein identifications (proteoforms) of 600 genes was obtained. Additionally, another set of experiments was performed where 16012 proteoforms coded by 4050 genes were identified by MS/MS according to their position in 96 gel sections (pixels). A special attention has been paid to the proteins that are the potential biomarkers of glioblastoma. The list of these biomarkers was compiled from literature. Next, we generated the graphs with theoretical and experimental information about proteoforms coded by the same gene. Such a virtual-experimental representation allowed better visualization of the state of these gene products. Many proteins, potential biomarkers of glioblastoma as well, are characterized by high numbers of protein species. We assume that these species could be a potential source of highly specific biomarkers of glioblastoma.
采用双向电泳(2DE)、质谱(ESI LC-MS/MS)和免疫检测对高级别胶质母细胞瘤细胞系中出现的不同蛋白质形态(蛋白质种类)进行了鉴定和定量分析。获得了包含600个基因的937个位点和1542个独特蛋白质鉴定(蛋白质形态)的2DE蛋白质图谱。此外,另一组实验采用MS/MS根据4050个基因编码的16012种蛋白质在96个凝胶切片(像素)中的位置进行鉴定。特别关注的是胶质母细胞瘤的潜在生物标志物蛋白质。这些生物标记物的列表是根据文献编制的。接下来,我们生成了由同一基因编码的蛋白质形式的理论和实验信息的图表。这样的虚拟实验表示可以更好地可视化这些基因产物的状态。许多蛋白质,以及胶质母细胞瘤的潜在生物标志物,都以大量的蛋白质种类为特征。我们认为这些物种可能是胶质母细胞瘤高度特异性生物标志物的潜在来源。
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Current Advances in Chemistry and Biochemistry Vol. 3
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