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Optimization of Matrigel concentration for salivary gland organoid culture 唾液腺类器官培养基质浓度的优化
Pub Date : 2023-10-25 DOI: 10.51335/organoid.2023.3.e14
Eunhoo Choi, Sangho Roh
Background: Research on salivary gland tissue is important, both for maintaining good oral health and for understanding the properties and functions of secretory cells with similar developmental mechanisms and functions. However, research on the pathogenesis of salivary gland disease and the development of therapeutic agents is very insufficient, and no studies have yet reported effective approaches to changes that occur according to the concentration of Matrigel, which is used to mimic the interactions between cells and the extracellular matrix, in three-dimensional organoid culture systems.Methods: We aimed to identify the most suitable concentration of Matrigel for analyzing the morphology and differentiation patterns of organoids prepared using salivary submandibular gland-derived cells after inducing differentiation with or without a specific chemical, Y-27632.Results: In both low and high Matrigel concentration conditions, morphological differences were found between the control group and the Y-27632 treatment group, and the budding structure was also significantly higher in the Y-27632 treatment group. However, more distinct patterns of differentiation appeared in the high-concentration condition.Conclusion: The concentration of Matrigel in the 3-dimensional organoid culture system had a significant effect on mimicking the actual in vivo environment.
背景:研究唾液腺组织对于维持口腔健康和了解具有相似发育机制和功能的分泌细胞的性质和功能具有重要意义。然而,唾液腺疾病发病机制的研究和治疗药物的开发非常不足,并且在三维类器官培养系统中,用于模拟细胞与细胞外基质之间相互作用的Matrigel浓度所发生的变化尚未有研究报道有效的方法。方法:我们的目的是确定最合适的Matrigel浓度,用于分析在Y-27632诱导或不诱导分化后由唾液颌下腺来源的细胞制备的类器官的形态学和分化模式。结果:在低浓度和高浓度条件下,对照组和Y-27632处理组在形态上存在差异,Y-27632处理组的出芽结构也显著高于Y-27632处理组。而在高浓度条件下,分化模式更为明显。结论:在三维类器官培养体系中添加浓度的Matrigel对模拟实际体内环境有显著影响。
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引用次数: 0
Natural biopolymer-based hydrogels as designer matrices for organoid cultures 基于天然生物聚合物的水凝胶作为类器官培养的设计基质
Pub Date : 2023-09-25 DOI: 10.51335/organoid.2023.3.e13
Yun Kee Jo
Matrigel, a mouse sarcoma-derived extract, is considered the gold standard for organoid cultures. However, it has several drawbacks, including inconsistent and ill-defined composition, varying quality between batches, and potential cancer-related health risks. These factors highlight the need to develop chemically defined alternatives to Matrigel. Natural biopolymers derived from living organisms have emerged as promising substitutes capable of creating chemically defined extracellular matrix (ECM)-mimicking materials to support organoids in a 3-dimensional (3D) environment. This article provides an overview of natural biopolymeric hydrogel-based bioengineering approaches for constructing 3D matrices resembling artificial ECM for organoid cultures. It discusses the latest developments in utilizing natural biopolymers to direct the growth, differentiation, and maturation of organoids, along with their translational applications in the fields of bioengineering and biomedicine. Additionally, the article offers perspectives on multidisciplinary research on natural biopolymer-based hydrogels for more practical applications as next-generation matrices for organoid cultures.
Matrigel是一种小鼠肉瘤提取物,被认为是类器官培养的黄金标准。然而,它有几个缺点,包括不一致和不明确的成分,批次之间的质量不同,以及潜在的与癌症相关的健康风险。这些因素突出了开发化学定义的替代品来替代Matrigel的必要性。从生物体中提取的天然生物聚合物已经成为有前途的替代品,能够创造化学定义的细胞外基质(ECM)-模拟材料,以支持三维(3D)环境中的类器官。本文概述了基于天然生物聚合物水凝胶的生物工程方法,用于构建类似于类器官培养的人工ECM的3D基质。讨论了利用天然生物聚合物指导类器官生长、分化和成熟的最新进展,以及它们在生物工程和生物医学领域的转化应用。此外,文章还展望了天然生物聚合物基水凝胶的多学科研究前景,以期作为类器官培养的下一代基质得到更广泛的应用。
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引用次数: 0
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Organoid (Online)
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