Pub Date : 2024-06-05DOI: 10.47616/jamrmhss.v5i1.505
Ahmed Mshari, Najwa M. Abu-Mejdad, Khairallah A. S. Mohammed
The current study was conducted to determine the prevalence of extended-spectrum β-lactamase (ESBL) in 78 drug-resistant clinical isolates (25 Klebsiella pneumoniae and 53 Escherichia coli strains) using phenotypic and molecular methods. The phenotypic method was performed using a double-disk synergy test (DDST), while the genotypic method screened for the blaSHV, blaCTX-M13U, and blaOXA-10 genes using specific primers. The phenotypic results showed that out of 53 tested strains of E. coli, 17 (32.07%) produced ESBL. Similarly, out of 25 tested strains of K. pneumoniae, 8 (32%) produced ESBL. Genotypic detection showed that in E. coli, the most abundant gene was SHV, present in 24 strains (45.28%), followed by blaOXA-10 in 23 strains (43.39%) and CTX-M-3 in 8 strains (15.09%). In K. pneumoniae, SHV was detected in 12 strains (48%), followed by OXA-10 and CTX-M-3, each found in 5 strains (20%).
{"title":"Prevalence of blaOXA-10, blaCTX-M-3 and SHV Genes among ESBL-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Clinical samples in Basra city, Iraq","authors":"Ahmed Mshari, Najwa M. Abu-Mejdad, Khairallah A. S. Mohammed","doi":"10.47616/jamrmhss.v5i1.505","DOIUrl":"https://doi.org/10.47616/jamrmhss.v5i1.505","url":null,"abstract":"The current study was conducted to determine the prevalence of extended-spectrum β-lactamase (ESBL) in 78 drug-resistant clinical isolates (25 Klebsiella pneumoniae and 53 Escherichia coli strains) using phenotypic and molecular methods. The phenotypic method was performed using a double-disk synergy test (DDST), while the genotypic method screened for the blaSHV, blaCTX-M13U, and blaOXA-10 genes using specific primers. The phenotypic results showed that out of 53 tested strains of E. coli, 17 (32.07%) produced ESBL. Similarly, out of 25 tested strains of K. pneumoniae, 8 (32%) produced ESBL. Genotypic detection showed that in E. coli, the most abundant gene was SHV, present in 24 strains (45.28%), followed by blaOXA-10 in 23 strains (43.39%) and CTX-M-3 in 8 strains (15.09%). In K. pneumoniae, SHV was detected in 12 strains (48%), followed by OXA-10 and CTX-M-3, each found in 5 strains (20%).","PeriodicalId":475828,"journal":{"name":"Journal of Asian multicultural research for medical and health science study","volume":"49 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141381789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Covid-19 pandemic, a rapidly spreading infectious disease, limits one's activities and reduces physical activity as a result of the adoption of social isolation to reduce the risk of transmission. As the Covid-19 pandemic spreads, staying physically active is crucial to maintaining mental wellness. This study aims to investigate the relationship between physical exercise and adolescent mental health in the Covid-19 epidemic. This research uses a cross-sectional design and is quantitative. Using a stratified random sampling technique, 246 persons were sampled at SMPN Padang City. The Self Reporting Questionnaire-20 (SRQ-20) and Physical Activity Questionnaire-Adolescent (PAQ-A) questionnaires were distributed online using google forms for this study's collection of physical activity and mental health data. The data were analyzed using the Chi-square test. Adolescent mental health was shown to be significantly correlated with physical exercise (p-value = 0.000; OR 2.660; CI 1.568-4.515). Teenagers are advised to enhance their physical activity levels and learn about physical activities that can help prevent mental health difficulties during the COVID-19 pandemic in order to help prevent problems.
{"title":"Adolescent Mental Health and Physical Activity During the COVID-19 Pandemic in Padang City","authors":"Susmiati Susmiati, Miftah Fauziyah, Mulyanti Roberto Muliantino","doi":"10.47616/jamrmhss.v4i2.391","DOIUrl":"https://doi.org/10.47616/jamrmhss.v4i2.391","url":null,"abstract":"The Covid-19 pandemic, a rapidly spreading infectious disease, limits one's activities and reduces physical activity as a result of the adoption of social isolation to reduce the risk of transmission. As the Covid-19 pandemic spreads, staying physically active is crucial to maintaining mental wellness. This study aims to investigate the relationship between physical exercise and adolescent mental health in the Covid-19 epidemic. This research uses a cross-sectional design and is quantitative. Using a stratified random sampling technique, 246 persons were sampled at SMPN Padang City. The Self Reporting Questionnaire-20 (SRQ-20) and Physical Activity Questionnaire-Adolescent (PAQ-A) questionnaires were distributed online using google forms for this study's collection of physical activity and mental health data. The data were analyzed using the Chi-square test. Adolescent mental health was shown to be significantly correlated with physical exercise (p-value = 0.000; OR 2.660; CI 1.568-4.515). Teenagers are advised to enhance their physical activity levels and learn about physical activities that can help prevent mental health difficulties during the COVID-19 pandemic in order to help prevent problems.","PeriodicalId":475828,"journal":{"name":"Journal of Asian multicultural research for medical and health science study","volume":" 42","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135293646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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