Introduction: Platelets are fragments of megakaryocytes circulating in the blood and its concentrates are therapeutic in substantial bleeding disorders. Efforts to ensure adequate product quality are required due to their short lifespan and lack of robustness. A descriptive longitudinal laboratory-based study was adopted in this study. The study aimed at determining platelets survival in stored platelet concentrates and evaluating thromboxane A2 for platelets function. Materials and Methods: Platelet concentrates were prepared manually using buffy coat, where about 50ml of concentrates suspended in plasma were allowed to rotate and agitate continuously on platelet agitator at room temperature (20-240C). Aliquots of 4ml each was collected serially for 9 days (day 0 to day 8) from 10 different platelet concentrates collected from 7 male blood donors and 3 female blood donors with age (mean± SD: 36 ± 7.14 years), weight (mean± SD: 66.8 ± 6.01kg), height (mean± SD: 163 ± 4.57cm). The samples were analyzed for platelets count, platelet distribution width (PDW), mean platelet volume (MPV), platelet-large cell ratio (P-LCR), using Automated Haematology analyzer (Sysmex XP-300) and thromboxane A2 using standard ELISA technique. Data analysis was carried out using mean, standard deviation as descriptive statistics and logistic regression as inferential statistics; and p <0.05 was considered as evidence of statistical significance. Results: Socio-demographic characteristics had no effect on all parameters estimated. There is variation in platelet count and platelet indices values in stored platelet concentrates compared with the baseline values and the survival of platelets in stored platelet concentrates was relatively stable till day 4 after preparation but depreciation surfaced from day 5 to day 8 compared to baseline values. The study also showed that the degree of deterioration of thromboxane A2 was highly significant at day 3 (p<0.05) while the best duration of storage for platelet concentrates in the study area is the first 3 days, though storage up to day 5 is acceptable (p>0.05). Conclusion: This study confirms thromboxane A2 as a marker for platelet functionality. The best duration of storage for platelet concentrates in the study area is the first two (2) days when no significant deterioration was observed.
{"title":"In-Vitro Assessment of Platelets Survival in Stored Platelet Concentrates in Ile-Ife, Nigeria","authors":"Abdulwaheed Adewale Ademosun, Musa Abidemi Muhibi, Tesleemah Oluwakemi Davies-Folorunso, Olufemi David Olaniyi, Nuryn Abdulganiy, Fatimat Adewumi Adedapo-Ismail, Yekeen Adebisi Kosamat, Mathew Folaranmi Olaniyan","doi":"10.59708/ajlhts.v2i3.2317","DOIUrl":"https://doi.org/10.59708/ajlhts.v2i3.2317","url":null,"abstract":"Introduction: Platelets are fragments of megakaryocytes circulating in the blood and its concentrates are therapeutic in substantial bleeding disorders. Efforts to ensure adequate product quality are required due to their short lifespan and lack of robustness. A descriptive longitudinal laboratory-based study was adopted in this study. The study aimed at determining platelets survival in stored platelet concentrates and evaluating thromboxane A2 for platelets function. Materials and Methods: Platelet concentrates were prepared manually using buffy coat, where about 50ml of concentrates suspended in plasma were allowed to rotate and agitate continuously on platelet agitator at room temperature (20-240C). Aliquots of 4ml each was collected serially for 9 days (day 0 to day 8) from 10 different platelet concentrates collected from 7 male blood donors and 3 female blood donors with age (mean± SD: 36 ± 7.14 years), weight (mean± SD: 66.8 ± 6.01kg), height (mean± SD: 163 ± 4.57cm). The samples were analyzed for platelets count, platelet distribution width (PDW), mean platelet volume (MPV), platelet-large cell ratio (P-LCR), using Automated Haematology analyzer (Sysmex XP-300) and thromboxane A2 using standard ELISA technique. Data analysis was carried out using mean, standard deviation as descriptive statistics and logistic regression as inferential statistics; and p <0.05 was considered as evidence of statistical significance. Results: Socio-demographic characteristics had no effect on all parameters estimated. There is variation in platelet count and platelet indices values in stored platelet concentrates compared with the baseline values and the survival of platelets in stored platelet concentrates was relatively stable till day 4 after preparation but depreciation surfaced from day 5 to day 8 compared to baseline values. The study also showed that the degree of deterioration of thromboxane A2 was highly significant at day 3 (p<0.05) while the best duration of storage for platelet concentrates in the study area is the first 3 days, though storage up to day 5 is acceptable (p>0.05). Conclusion: This study confirms thromboxane A2 as a marker for platelet functionality. The best duration of storage for platelet concentrates in the study area is the first two (2) days when no significant deterioration was observed.","PeriodicalId":476186,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136342289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.59708/ajlhts.v2i3.0001
Zaccheaus Awortu Jeremiah
Editorial
编辑
{"title":"AJLHTS: KEEPING PACE WITH JPPS","authors":"Zaccheaus Awortu Jeremiah","doi":"10.59708/ajlhts.v2i3.0001","DOIUrl":"https://doi.org/10.59708/ajlhts.v2i3.0001","url":null,"abstract":"Editorial","PeriodicalId":476186,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.59708/ajlhts.v2i3.2329
P.A Akpan, V.E Njar, E.E Volunteer
Introduction: Blood for transfusion or biopharmaceutical medication is obtained through blood donation. It is an indispensable component of health that contributes to saving lives since blood/blood products are unique. A major source of safe blood is voluntary non-remunerated blood donors. Considering the role of teachers in the education of young people in the populace, this study aims to provide information on knowledge and attitude toward blood donation and the prevalence of Hepatitis B and C among secondary school teachers in Calabar, Nigeria. Methods: With ethical approval and informed consent obtained, a total of 200 apparently healthy teachers and staff were recruited from five secondary schools in Calabar. Structured questionnaires were pre-tested among twenty (20) staff of the University of Calabar before being administered to the study subjects. Blood was collected and screened for the presence of hepatitis B and C using the standard strip method. Data obtained were analyzed using the Chi-square test on SPSS version 21 and p<0.05 was considered statistically significant. Results: The study subjects comprised males (49.5%) and females (50.5%) with 38% being between the ages of 27-37 years. The majority (67.5%) had attained tertiary education while the remaining 32.5% had secondary education. The prevalence of Hepatitis B and C was observed to be 10% and 4% respectively among the study population and this is comparable to the prevalence in the general population. Ninety-five percent of participants think voluntary blood donation is good with 100% affirming that it is important yet only 10% had actually donated blood; eight percent had received blood transfusion previously. A good number (87% and 65%) were willing to donate for a family member and for a stranger in case of emergency. Eighty-four and a half percent of respondents think that blood donation is beneficial, 78% think there is a lack of awareness while 70% would advise others to donate blood voluntarily. Of the 200 participants, 66% believe blood donation to be a civic duty yet 82% and 85.5% respectively were of the opinion that blood donors should be paid or given gifts; indeed 83% agreed they would donate blood if they are paid. None of the respondents were against blood donation however 13% believed it poses a risk of collapse or death to the donor. Conclusions: This study has shown that the prevalence of Hepatitis B and C among secondary school teachers in Calabar is 10% and 4% respectively. Secondary school teachers in the study area have fair knowledge of blood donation, and agree that it is important but are not willing to donate without renumeration. Lack of voluntary non-renumerated blood donation leads to a shortage of safe blood for transfusion and will promote commercial donation with associated risks. There is a need for regular awareness campaigns and blood drives among the populace.
{"title":"Prevalence of hepatitis B and C, knowledge and attitude towards voluntary blood donation among secondary school teachers in Calabar, Nigeria","authors":"P.A Akpan, V.E Njar, E.E Volunteer","doi":"10.59708/ajlhts.v2i3.2329","DOIUrl":"https://doi.org/10.59708/ajlhts.v2i3.2329","url":null,"abstract":"Introduction: Blood for transfusion or biopharmaceutical medication is obtained through blood donation. It is an indispensable component of health that contributes to saving lives since blood/blood products are unique. A major source of safe blood is voluntary non-remunerated blood donors. Considering the role of teachers in the education of young people in the populace, this study aims to provide information on knowledge and attitude toward blood donation and the prevalence of Hepatitis B and C among secondary school teachers in Calabar, Nigeria. Methods: With ethical approval and informed consent obtained, a total of 200 apparently healthy teachers and staff were recruited from five secondary schools in Calabar. Structured questionnaires were pre-tested among twenty (20) staff of the University of Calabar before being administered to the study subjects. Blood was collected and screened for the presence of hepatitis B and C using the standard strip method. Data obtained were analyzed using the Chi-square test on SPSS version 21 and p<0.05 was considered statistically significant. Results: The study subjects comprised males (49.5%) and females (50.5%) with 38% being between the ages of 27-37 years. The majority (67.5%) had attained tertiary education while the remaining 32.5% had secondary education. The prevalence of Hepatitis B and C was observed to be 10% and 4% respectively among the study population and this is comparable to the prevalence in the general population. Ninety-five percent of participants think voluntary blood donation is good with 100% affirming that it is important yet only 10% had actually donated blood; eight percent had received blood transfusion previously. A good number (87% and 65%) were willing to donate for a family member and for a stranger in case of emergency. Eighty-four and a half percent of respondents think that blood donation is beneficial, 78% think there is a lack of awareness while 70% would advise others to donate blood voluntarily. Of the 200 participants, 66% believe blood donation to be a civic duty yet 82% and 85.5% respectively were of the opinion that blood donors should be paid or given gifts; indeed 83% agreed they would donate blood if they are paid. None of the respondents were against blood donation however 13% believed it poses a risk of collapse or death to the donor. Conclusions: This study has shown that the prevalence of Hepatitis B and C among secondary school teachers in Calabar is 10% and 4% respectively. Secondary school teachers in the study area have fair knowledge of blood donation, and agree that it is important but are not willing to donate without renumeration. Lack of voluntary non-renumerated blood donation leads to a shortage of safe blood for transfusion and will promote commercial donation with associated risks. There is a need for regular awareness campaigns and blood drives among the populace.","PeriodicalId":476186,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136342086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.59708/ajlhts.v2i3.2321
Serekara Gideon Christian, Sophia Agbara
Introduction: This study aims to determine the association of ABO/Rh Blood groups and haemoglobin genotype with Helicobacter pylori infection among indigenes of Wiyaakara, Ogoni, Rivers State. This is a cross-sectional study carried out in Wiyaakara, Khana Local Government Area in Rivers State to determine the prevalence of Helicobacter pylori infection and its association with ABO/Rh blood group and haemoglobin genotype.The study was carried out amongst indigenes of Wiyaakara village in Khana LGA of Rivers State, Nigeria. Wiyaakara is part of Ogoni. Materials and Methods: Determination of ABO/Rh blood group was done using tile method. Haemoglobin genotype was determined using cellulose acetate paper electrophoresis while determination of Helicobacter pylori infection was done using the rapid diagnostic test strip method. Results: Out of 130 participants, 48.5% tested positive for Helicobacter pylori infection. Amongst those that tested positive, 68% were females while 32% were males. In ABO blood grouping, blood group O recorded the highest infectivity rate, followed by blood group A, blood group B and AB. Rh positive individuals recorded high frequency of occurrence than Rh negative. Haemoglobin genotype AA recorded high frequency of occurrence than that of AS. Based on odd ratios, the risk for the studied subjects to be infected with Helicobacter pylori was in the order of B>A>O (odd ratios: A = 1.78, B = 9.01, O = 0.54), considering the fact that AB blood group subjects was negligible due to fewer number of subjects (5). Haemoglobin AS subjects were more prone to having Helicobacter pylori than AA subjects. Conclusion: The study has revealed that blood group B individual are more at risk of being infected with Helicobacter pylori in comparison to other ABO blood groups. Additionally, those with haemoglobin AS genotype were also at risk of being infected with Helicobacter pylori than those with haemoglobin AA genotype.
简介:本研究旨在确定ABO/Rh血型和血红蛋白基因型与Wiyaakara, Ogoni, Rivers State土著人幽门螺杆菌感染的关系。这是一项在河流州Khana地方政府区的Wiyaakara进行的横断面研究，目的是确定幽门螺杆菌感染的患病率及其与ABO/Rh血型和血红蛋白基因型的关系。这项研究是在尼日利亚河流州Khana LGA的Wiyaakara村的土著人中进行的。维亚卡拉是奥戈尼的一部分。材料与方法:采用瓦片法测定ABO/Rh血型。采用醋酸纤维素纸电泳法检测血红蛋白基因型，采用快速诊断试纸条法检测幽门螺杆菌感染。结果:在130名参与者中，有48.5%的人检测出幽门螺杆菌感染阳性。在检测呈阳性的人中，68%为女性，32%为男性。在ABO血型中，O型血的感染率最高，其次是A型血、B型血和AB型血，Rh阳性个体的发生频率高于Rh阴性个体。血红蛋白基因型AA的发生率高于AS。考虑到AB血型受试者人数较少，可以忽略不计(5)，基于奇数比，研究对象感染幽门螺杆菌的风险为B>A>O(奇数比:A = 1.78, B = 9.01, O = 0.54)。血红蛋白AS受试者比AA型受试者更容易感染幽门螺杆菌。结论:研究表明B血型个体与其他ABO血型个体相比，感染幽门螺杆菌的风险更高。此外，血红蛋白AS基因型患者感染幽门螺杆菌的风险也高于血红蛋白AA基因型患者。
{"title":"ABO, Rh Blood Groups and Haemoglobin Genotype Associations with Helicobacter pylori Infection amongst Indigenes of Wiyaakara, Rivers State","authors":"Serekara Gideon Christian, Sophia Agbara","doi":"10.59708/ajlhts.v2i3.2321","DOIUrl":"https://doi.org/10.59708/ajlhts.v2i3.2321","url":null,"abstract":"Introduction: This study aims to determine the association of ABO/Rh Blood groups and haemoglobin genotype with Helicobacter pylori infection among indigenes of Wiyaakara, Ogoni, Rivers State. This is a cross-sectional study carried out in Wiyaakara, Khana Local Government Area in Rivers State to determine the prevalence of Helicobacter pylori infection and its association with ABO/Rh blood group and haemoglobin genotype.The study was carried out amongst indigenes of Wiyaakara village in Khana LGA of Rivers State, Nigeria. Wiyaakara is part of Ogoni. Materials and Methods: Determination of ABO/Rh blood group was done using tile method. Haemoglobin genotype was determined using cellulose acetate paper electrophoresis while determination of Helicobacter pylori infection was done using the rapid diagnostic test strip method. Results: Out of 130 participants, 48.5% tested positive for Helicobacter pylori infection. Amongst those that tested positive, 68% were females while 32% were males. In ABO blood grouping, blood group O recorded the highest infectivity rate, followed by blood group A, blood group B and AB. Rh positive individuals recorded high frequency of occurrence than Rh negative. Haemoglobin genotype AA recorded high frequency of occurrence than that of AS. Based on odd ratios, the risk for the studied subjects to be infected with Helicobacter pylori was in the order of B>A>O (odd ratios: A = 1.78, B = 9.01, O = 0.54), considering the fact that AB blood group subjects was negligible due to fewer number of subjects (5). Haemoglobin AS subjects were more prone to having Helicobacter pylori than AA subjects. Conclusion: The study has revealed that blood group B individual are more at risk of being infected with Helicobacter pylori in comparison to other ABO blood groups. Additionally, those with haemoglobin AS genotype were also at risk of being infected with Helicobacter pylori than those with haemoglobin AA genotype.","PeriodicalId":476186,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136342089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.59708/ajlhts.v2i3.2330
H.A Buhari, A Sagir, S.A Akuyam, O Erhabor, A.A Panti, N Garba
Background: Blood group antigens have been used to evaluate ethnic diversity of human populations and they also play most important roles in pregnancy and blood transfusion. Knowledge of antigen frequencies is important to assess the risk of alloimmunization and to guide the probability of finding antigen-negative donor blood. This study was aimed at determining the frequencies of ABO, Rh and Kell blood group antigens phenotype among pregnant women. Materials and Methods: The study was cross sectional in nature conducted among 1,250 consecutively recruited pregnant women in the department of Obstetrics and Gynaecology, antenatal clinic in Sokoto from January 2020 to September 2020. The blood grouping were determined using standard tube techniques for ABO while column agglutination card was used for Rh C, E, c, e and Kell blood groups. The data were collected, and calculations were done to determine the frequencies of ABO, Rh, and Kell blood group antigens and chi square was used to determine statistical significance . Results: The distribution of the ABO blood group revealed that 48.5% were group O, 27.3% were group B, 19.4% were group A and 4.8% were group AB. Out the subjects investigated, 93.1%, 30.2%, 24.6% , 90.2% and 97.6%were RhD, RhC, RhE, Rhc and Rhe positive respectively while 2.4% were Kell positive while 97.6% were Kell negative. ABO antigens has statistically significant correlation with Kell antigens. Conclusion: There is phenotypic variability of ABO and Rh blood group antigens with low prevalence of Kell antigen that has statistical relationship with ABO antigen. Incorporation of blood group phenotyping as premarital screening may reduce the prevalence of alloantibody formation.
{"title":"ABO, Rh, and Kell Blood Group Antigen Frequencies among Pregnant Women in Sokoto, Nigeria.","authors":"H.A Buhari, A Sagir, S.A Akuyam, O Erhabor, A.A Panti, N Garba","doi":"10.59708/ajlhts.v2i3.2330","DOIUrl":"https://doi.org/10.59708/ajlhts.v2i3.2330","url":null,"abstract":"Background: Blood group antigens have been used to evaluate ethnic diversity of human populations and they also play most important roles in pregnancy and blood transfusion. Knowledge of antigen frequencies is important to assess the risk of alloimmunization and to guide the probability of finding antigen-negative donor blood. This study was aimed at determining the frequencies of ABO, Rh and Kell blood group antigens phenotype among pregnant women. Materials and Methods: The study was cross sectional in nature conducted among 1,250 consecutively recruited pregnant women in the department of Obstetrics and Gynaecology, antenatal clinic in Sokoto from January 2020 to September 2020. The blood grouping were determined using standard tube techniques for ABO while column agglutination card was used for Rh C, E, c, e and Kell blood groups. The data were collected, and calculations were done to determine the frequencies of ABO, Rh, and Kell blood group antigens and chi square was used to determine statistical significance . Results: The distribution of the ABO blood group revealed that 48.5% were group O, 27.3% were group B, 19.4% were group A and 4.8% were group AB. Out the subjects investigated, 93.1%, 30.2%, 24.6% , 90.2% and 97.6%were RhD, RhC, RhE, Rhc and Rhe positive respectively while 2.4% were Kell positive while 97.6% were Kell negative. ABO antigens has statistically significant correlation with Kell antigens. Conclusion: There is phenotypic variability of ABO and Rh blood group antigens with low prevalence of Kell antigen that has statistical relationship with ABO antigen. Incorporation of blood group phenotyping as premarital screening may reduce the prevalence of alloantibody formation.","PeriodicalId":476186,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136342090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Red cell antigens alongside red cell indices provide an essential support to the diagnosis and monitoring of haematological diseases while the erythrocyte sedimentation rate (ESR) indicates and monitor an increase in inflammatory activity within the body. This study aims to determine Rh-e antigen, Erythrocyte Sedimentation Rate and Red cell indices values in apparently healthy undergraduate students of the Rivers State University, Port Harcourt, Nigeria. Material and Methods: One hundred and fifty (150) undergraduate students aged between 17-28years were enrolled in the study and standard venipuncture technique used to collect 5ml of blood. Determination of the Rh-e antigen was carried out using anti-e monoclonal antibodies (Lorne Diagnostics UK), Red cell indices obtained using BC 5000 Mindray Haematology Analyser and ESR by Westergren method. Results: Among the 150 subjects, 130 (86.6%) were positive while 20 (13.3%) were negative for Rh-e antigen. The mean±SD of the mean cell volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), red cell distribution width co-efficient of variation (RDW-CV), red cell distribution width-standard deviation (RDW-SD) and erythrocyte sedimentation rate (ESR) were 83.12 ± 10.74, 31.13 ± 3.25, 34.00 ± 3.32, 13.32 ± 1.61, 39.70 ± 2.26 and 30.36 ± 2.15 in the same order, while the reference ranges were 61.64-104.6 for MCV, 24.63-37.63 for MCH, 27.36–40.64 for MCHC, 10.10–16.54 for RDW (CV), 35.18–44.22 for RDW (SD) and 26.06-34.66 for ESR. Gender had no effect on MCV (p=0.3007) and MCHC (p=0.1436) but significant effect on MCH (p=0.030), RDW-CV (p<0.001), RDW-SD (p=0.0005) and ESR (p=0.036) with a perfect positive correlation (r=1) between all the studied parameters. Conclusion: This study revealed a high (86.6%) prevalence for Rh-e antigen, established normal reference ranges for red cell indices and erythrocyte sedimentation rates and showed that gender has significant effects on MCH, RDW-CV, RDW-SD and ESR among healthy undergraduate students of Rivers State University. These findings are of clinical and research relevance.
{"title":"Frequency of Rh-e Antigen and reference values of Erythrocyte Sedimentation Rate and Red Cell Indices in an Undergraduate Students’ population in Port Harcourt, Nigeria.","authors":"Ransom Baribefii Jacob, Chizarum Boms, Orokwu Eziaku Chukwuigwe-Igbere, Goodnews Nkabari Nwika","doi":"10.59708/ajlhts.v2i3.2327","DOIUrl":"https://doi.org/10.59708/ajlhts.v2i3.2327","url":null,"abstract":"Introduction: Red cell antigens alongside red cell indices provide an essential support to the diagnosis and monitoring of haematological diseases while the erythrocyte sedimentation rate (ESR) indicates and monitor an increase in inflammatory activity within the body. This study aims to determine Rh-e antigen, Erythrocyte Sedimentation Rate and Red cell indices values in apparently healthy undergraduate students of the Rivers State University, Port Harcourt, Nigeria. Material and Methods: One hundred and fifty (150) undergraduate students aged between 17-28years were enrolled in the study and standard venipuncture technique used to collect 5ml of blood. Determination of the Rh-e antigen was carried out using anti-e monoclonal antibodies (Lorne Diagnostics UK), Red cell indices obtained using BC 5000 Mindray Haematology Analyser and ESR by Westergren method. Results: Among the 150 subjects, 130 (86.6%) were positive while 20 (13.3%) were negative for Rh-e antigen. The mean±SD of the mean cell volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), red cell distribution width co-efficient of variation (RDW-CV), red cell distribution width-standard deviation (RDW-SD) and erythrocyte sedimentation rate (ESR) were 83.12 ± 10.74, 31.13 ± 3.25, 34.00 ± 3.32, 13.32 ± 1.61, 39.70 ± 2.26 and 30.36 ± 2.15 in the same order, while the reference ranges were 61.64-104.6 for MCV, 24.63-37.63 for MCH, 27.36–40.64 for MCHC, 10.10–16.54 for RDW (CV), 35.18–44.22 for RDW (SD) and 26.06-34.66 for ESR. Gender had no effect on MCV (p=0.3007) and MCHC (p=0.1436) but significant effect on MCH (p=0.030), RDW-CV (p<0.001), RDW-SD (p=0.0005) and ESR (p=0.036) with a perfect positive correlation (r=1) between all the studied parameters. Conclusion: This study revealed a high (86.6%) prevalence for Rh-e antigen, established normal reference ranges for red cell indices and erythrocyte sedimentation rates and showed that gender has significant effects on MCH, RDW-CV, RDW-SD and ESR among healthy undergraduate students of Rivers State University. These findings are of clinical and research relevance.","PeriodicalId":476186,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136342290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.59708/ajlhts.v2i3.2322
S. A Saeed, I.R Maryam, F.P Udomah, T Oduola, H.M Alhassan, A.E Ahmad, Y Muhammad, A.Y Armiyau, Y Aminu, M.B Adamu
Background: von Willebrand Factor and factor VIII have been implicated in the vulnerability of causing hypertension which is related/common to some of the ABO blood group antigens. However, the precise mechanism/role of the von Willebrand Factor and factor VIII in hypertension is unknown. The study is a comparative study that is aimed to determine the association of ABO red cell antigen, von Willebrand factor, Factor VIII, and Platelets among hypertensive patients in Kaduna, Nigeria. Methods: To determine this association, fifty-five (55) hypertensive patients and 28 controls were recruited. A purposive sampling technique was employed in selecting the study participants. Determination of the ABO blood group, vWF, FVIII, and platelets was carried out to establish the frequency and the association of ABO blood type with hypertension. Results: It was established that blood group O (43%) has the highest distribution followed by A, B, and AB (6%) the least. There was no significant difference in vWF(ng/L), FVIII(pg/ml), and platelet count(109/l) between the patients and the controls (454.9 and 456.2), (242.0 and 228.4) and (238.0 and 213.0) respectively, p>0.05. The correlation studies showed a strong association between vWF and FVIII (r - 0.544, P<0.0001). There was a significant difference between males’ and females’ vWF and FVIII (P=0.0013 and 0.0029 respectively), indicating females had a higher level of vWF and FVIII, and therefore at higher risk of developing hypertension. Women should therefore be screened for these parameters as a matter of routine. Conclusion: The risk of developing hypertension based on the parameters considered in this study is independent of the ABO blood group of an individual. There is a need for further studies, to confirm or rule out these findings.
{"title":"ABO Red Cell Antigens, von Willebrand Factor, Factor VIII and Platelet counts among Hypertensive Patients In Kaduna Metropolis, Kaduna.","authors":"S. A Saeed, I.R Maryam, F.P Udomah, T Oduola, H.M Alhassan, A.E Ahmad, Y Muhammad, A.Y Armiyau, Y Aminu, M.B Adamu","doi":"10.59708/ajlhts.v2i3.2322","DOIUrl":"https://doi.org/10.59708/ajlhts.v2i3.2322","url":null,"abstract":"Background: von Willebrand Factor and factor VIII have been implicated in the vulnerability of causing hypertension which is related/common to some of the ABO blood group antigens. However, the precise mechanism/role of the von Willebrand Factor and factor VIII in hypertension is unknown. The study is a comparative study that is aimed to determine the association of ABO red cell antigen, von Willebrand factor, Factor VIII, and Platelets among hypertensive patients in Kaduna, Nigeria. Methods: To determine this association, fifty-five (55) hypertensive patients and 28 controls were recruited. A purposive sampling technique was employed in selecting the study participants. Determination of the ABO blood group, vWF, FVIII, and platelets was carried out to establish the frequency and the association of ABO blood type with hypertension. Results: It was established that blood group O (43%) has the highest distribution followed by A, B, and AB (6%) the least. There was no significant difference in vWF(ng/L), FVIII(pg/ml), and platelet count(109/l) between the patients and the controls (454.9 and 456.2), (242.0 and 228.4) and (238.0 and 213.0) respectively, p>0.05. The correlation studies showed a strong association between vWF and FVIII (r - 0.544, P<0.0001). There was a significant difference between males’ and females’ vWF and FVIII (P=0.0013 and 0.0029 respectively), indicating females had a higher level of vWF and FVIII, and therefore at higher risk of developing hypertension. Women should therefore be screened for these parameters as a matter of routine. Conclusion: The risk of developing hypertension based on the parameters considered in this study is independent of the ABO blood group of an individual. There is a need for further studies, to confirm or rule out these findings.","PeriodicalId":476186,"journal":{"name":"African Journal of Laboratory Haematology and Transfusion Science","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136342291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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