Pub Date : 2023-10-31DOI: 10.22302/e-journalmenaraperkebunan.v91i2.552
Nisfatin Shofiana, Titi Candra Sunarti, Anja Meryandini
Cocoa production in Indonesia is increasing from year to year. Cocoa production increased has an impact on increasing waste cocoa pod and pulp. Cocoa pod contains lignocellulose, while cocoa pulp contains many sugars. The composition of cocoa pod and pulp allows the cellulolytic microbe to grow. This study aims to perform microbial selection from fermented cocoa and sugar cane can grow in cocoa pod and pulp (Theobroma cacao). The selection method using the CMC 1%, pod 1%, pulp 1%, and pod 0,5%+pulp 0,5% media. Then, the analysis of reducing sugar using DNS method, while the total sugar analysis using phenol-H2SO4 method. Sugars of hydrolisis result were analyzed quantitatively using TLC. The results of microbial selection on CMC 1%, pod %, pulp 1%, and pod 0,5%+pulp 0,5% media obtained TBT 3.2 have the largest cellulolytic index on the pod 1% media. Isolates TBT 3.2 grown on pod 1% medium produce reducing sugar is 4,965 mg/mL at 30 hours sampling with highest total sugar at 6 hours sampling is 9,789 mg/mL. The types of sugars identified using the TLC are mannose, galactose, glucose, and selobiose.
{"title":"Viabilitas mikroba selulolitik pada media pod dan pulp kakao (Theobroma cacao L.)","authors":"Nisfatin Shofiana, Titi Candra Sunarti, Anja Meryandini","doi":"10.22302/e-journalmenaraperkebunan.v91i2.552","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.552","url":null,"abstract":"Cocoa production in Indonesia is increasing from year to year. Cocoa production increased has an impact on increasing waste cocoa pod and pulp. Cocoa pod contains lignocellulose, while cocoa pulp contains many sugars. The composition of cocoa pod and pulp allows the cellulolytic microbe to grow. This study aims to perform microbial selection from fermented cocoa and sugar cane can grow in cocoa pod and pulp (Theobroma cacao). The selection method using the CMC 1%, pod 1%, pulp 1%, and pod 0,5%+pulp 0,5% media. Then, the analysis of reducing sugar using DNS method, while the total sugar analysis using phenol-H2SO4 method. Sugars of hydrolisis result were analyzed quantitatively using TLC. The results of microbial selection on CMC 1%, pod %, pulp 1%, and pod 0,5%+pulp 0,5% media obtained TBT 3.2 have the largest cellulolytic index on the pod 1% media. Isolates TBT 3.2 grown on pod 1% medium produce reducing sugar is 4,965 mg/mL at 30 hours sampling with highest total sugar at 6 hours sampling is 9,789 mg/mL. The types of sugars identified using the TLC are mannose, galactose, glucose, and selobiose.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":" 37","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135863127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-31DOI: 10.22302/e-journalmenaraperkebunan.v91i2.536
Loekas Soesanto, Murti Ragil Wisnu Sastyawan, Abdul Manan, Endang Mugiastuti
Control of billygoat (Ageratum conyzoides L) currently uses herbicides, on the other hand, control using pathogenic fungi is environmentally friendly, but for mass propagation and storage, alternative media are needed. The aim of the study was to determine the best type of alternative media for fungal growth and shelf life, as well as its effectiveness against billygoat. The treatment tested involves a combination of two factors: the type of pathogenic fungi (Curvularia lunata or Fusarium oxysporum) and the type of medium (rice washing water or tofu liquid waste). A completely randomized design was used for the in vitro test, while in planta test used a randomized block design with each experimental unit repeated five times. The observed variables were conidia density, number of colonies, incubation period, disease symptoms, disease intensity, and area under the disease progress curve (AUDPC), as well as weed height, number of leaves, fresh and dry shoot, and root weights. The results showed that the conidia density of F. oxysporum was 57% better in rice washing water than in tofu wastewater. The best shelf life for the fungus was four weeks. The use of rice washing water for F. oxysporum and C. lunata effectively delayed the incubation period by 77 and 71% respectively, suppressed disease intensity by 90 and 88%, and AUDPC by 94 and 93% compared to the control. The F. oxysporum grown on rice washing water media was able to reduce the number of leaves, fresh and dry weight of billygoat by 25, 30, and 20% compared to the control, respectively.
{"title":"Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L)","authors":"Loekas Soesanto, Murti Ragil Wisnu Sastyawan, Abdul Manan, Endang Mugiastuti","doi":"10.22302/e-journalmenaraperkebunan.v91i2.536","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.536","url":null,"abstract":"Control of billygoat (Ageratum conyzoides L) currently uses herbicides, on the other hand, control using pathogenic fungi is environmentally friendly, but for mass propagation and storage, alternative media are needed. The aim of the study was to determine the best type of alternative media for fungal growth and shelf life, as well as its effectiveness against billygoat. The treatment tested involves a combination of two factors: the type of pathogenic fungi (Curvularia lunata or Fusarium oxysporum) and the type of medium (rice washing water or tofu liquid waste). A completely randomized design was used for the in vitro test, while in planta test used a randomized block design with each experimental unit repeated five times. The observed variables were conidia density, number of colonies, incubation period, disease symptoms, disease intensity, and area under the disease progress curve (AUDPC), as well as weed height, number of leaves, fresh and dry shoot, and root weights. The results showed that the conidia density of F. oxysporum was 57% better in rice washing water than in tofu wastewater. The best shelf life for the fungus was four weeks. The use of rice washing water for F. oxysporum and C. lunata effectively delayed the incubation period by 77 and 71% respectively, suppressed disease intensity by 90 and 88%, and AUDPC by 94 and 93% compared to the control. The F. oxysporum grown on rice washing water media was able to reduce the number of leaves, fresh and dry weight of billygoat by 25, 30, and 20% compared to the control, respectively.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135872434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Among consumed-vegetable oils, RBDPO (refined, bleached and deodorized palm oil) is indicated containing higher level of 3-MCPDE and GE, which have been classified as potentially carcinogenic and/or genotoxic to human. Those contaminants in RBDPO are formed during deodorization process with high temperature and also correlate with chloride content and quality of CPO as raw material. The maximum limit for 3-MCPDE and GE content in vegetable oil has been set at 2500 and 1000 µg kg-1, and as low as 750 and 500 µg kg-1 when used for production of baby food and cereal-based foods for infants and young children. The mitigation of 3-MCPDE and GE in RBDPO is quite challenging for palm oil refineries in producing a good quality of palm oil in term of sensory, stability, safety, and nutritional value, especially in Indonesia. It has been proven that modification of conventional refining process can reduce the 3-MCPDE and GE content. They are including pre-treatment of CPO by water washing to reduce the chloride content, alkali neutralization to remove any acids present in the oil prior to deodorization, using neutral bleaching earth, adding antioxidants after bleaching to reduce free radical-mediated formation of 3-MCPDE and GE, lowering the deodorization temperature with a longer time, and even double bleaching and/or double deodorization of the palm oil. Post-refining treatment using specific adsorbents is also applied to remove the 3-MCPDE and GE. However, palm oil refineries have to apply combination of several mitigation strategies to adequately reduce the levels of 3-MCPDE and GE, including using good quality of CPO with low FFA, chloride, and oxidized components content.
{"title":"Mitigation of 3-MCPDE and GE in palm oil in Indonesia","authors":"Jenny Elisabeth","doi":"10.22302/mp.v91i2.549","DOIUrl":"https://doi.org/10.22302/mp.v91i2.549","url":null,"abstract":"Among consumed-vegetable oils, RBDPO (refined, bleached and deodorized palm oil) is indicated containing higher level of 3-MCPDE and GE, which have been classified as potentially carcinogenic and/or genotoxic to human. Those contaminants in RBDPO are formed during deodorization process with high temperature and also correlate with chloride content and quality of CPO as raw material. The maximum limit for 3-MCPDE and GE content in vegetable oil has been set at 2500 and 1000 µg kg-1, and as low as 750 and 500 µg kg-1 when used for production of baby food and cereal-based foods for infants and young children. The mitigation of 3-MCPDE and GE in RBDPO is quite challenging for palm oil refineries in producing a good quality of palm oil in term of sensory, stability, safety, and nutritional value, especially in Indonesia. It has been proven that modification of conventional refining process can reduce the 3-MCPDE and GE content. They are including pre-treatment of CPO by water washing to reduce the chloride content, alkali neutralization to remove any acids present in the oil prior to deodorization, using neutral bleaching earth, adding antioxidants after bleaching to reduce free radical-mediated formation of 3-MCPDE and GE, lowering the deodorization temperature with a longer time, and even double bleaching and/or double deodorization of the palm oil. Post-refining treatment using specific adsorbents is also applied to remove the 3-MCPDE and GE. However, palm oil refineries have to apply combination of several mitigation strategies to adequately reduce the levels of 3-MCPDE and GE, including using good quality of CPO with low FFA, chloride, and oxidized components content.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"435 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135863525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-30DOI: 10.22302/e-journalmenaraperkebunan.v91i2.551
Masna Maya Sinta, Lailia Zubaidah, Rizka Tamania Saptari, Imron Riyadi, Galuh Wening Permatasari, Riza Arief Putranto, Annisa A Aksa, Larasati D Mahardhika, Yuli Setiawati, Hayati Minarsih, Ernayunita Ernayunita
Contamination in the in vitro culture is a critical problem causing the failure of seed production. Contamination in the oil palm plantlet is detrimental, considering that oil palm propagation is difficult and takes a long time. This research aimed to study the effect of sterilization during acclimatization of the contaminated oil palm plantlets by fungi on viability and to determine the optimum viability achieved from the contaminated materials. The materials used were contaminated plantlets of oil palm with roots, four leaves, and a height of about 17 cm. The plantlets were removed from the tube and cleaned with running tap water, then were sterilized, with treatments P1: soaking in benomyl-mancozeb-sodium hypochlorite and mannitol and rinsing with aquadest, P2: soaking in benomyl-mancozeb, P3: soaking in mancozeb. Cleaning plantlets under running tap water was carried out as a control treatment. The results showed that at 10 weeks after acclimatization, the survival rate of plantlets in each treatment (P1, P2, and P3) was significantly higher than that of the control. Sterilization methods affect the time new leaves emerge, leaf condition after sterilization treatment, and shoot height. The lowest fungal contamination after treatments was found in P2, followed by P3. After 3 months, plantlet survival rate decreased, with the highest survival rate in treatment P3 (32.3%) followed by treatment P2 (22.5%). In conclusion, acclimatization of contaminated oil palm plantlets can be carried out using a suitable sterilization treatment. Sterilization affects the survival rate and growth of in vitro-contaminated oil palm plantlets during acclimatization.
{"title":"Sterilization method of contaminated oil palm plantlets affects the survival rate during acclimatization","authors":"Masna Maya Sinta, Lailia Zubaidah, Rizka Tamania Saptari, Imron Riyadi, Galuh Wening Permatasari, Riza Arief Putranto, Annisa A Aksa, Larasati D Mahardhika, Yuli Setiawati, Hayati Minarsih, Ernayunita Ernayunita","doi":"10.22302/e-journalmenaraperkebunan.v91i2.551","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.551","url":null,"abstract":"Contamination in the in vitro culture is a critical problem causing the failure of seed production. Contamination in the oil palm plantlet is detrimental, considering that oil palm propagation is difficult and takes a long time. This research aimed to study the effect of sterilization during acclimatization of the contaminated oil palm plantlets by fungi on viability and to determine the optimum viability achieved from the contaminated materials. The materials used were contaminated plantlets of oil palm with roots, four leaves, and a height of about 17 cm. The plantlets were removed from the tube and cleaned with running tap water, then were sterilized, with treatments P1: soaking in benomyl-mancozeb-sodium hypochlorite and mannitol and rinsing with aquadest, P2: soaking in benomyl-mancozeb, P3: soaking in mancozeb. Cleaning plantlets under running tap water was carried out as a control treatment. The results showed that at 10 weeks after acclimatization, the survival rate of plantlets in each treatment (P1, P2, and P3) was significantly higher than that of the control. Sterilization methods affect the time new leaves emerge, leaf condition after sterilization treatment, and shoot height. The lowest fungal contamination after treatments was found in P2, followed by P3. After 3 months, plantlet survival rate decreased, with the highest survival rate in treatment P3 (32.3%) followed by treatment P2 (22.5%). In conclusion, acclimatization of contaminated oil palm plantlets can be carried out using a suitable sterilization treatment. Sterilization affects the survival rate and growth of in vitro-contaminated oil palm plantlets during acclimatization.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"109 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136068973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-26DOI: 10.22302/e-journalmenaraperkebunan.v91i2.545
Muhammad Abdul Aziz, Ciptadi Ahmad Yusup, Siswanto Siswanto, Djoko Santoso, Priyono Priyono, Happy Widiastuti
Sucrosin technology, have been tested in more than 10,000 ha of sugarcane planting area, resulted in highly variable increases in productivity. However, fulfilling standard operational procedures when implemented on a wide scale is complicated, so it needs to be simplified regarding product formulation and application techniques. This study aimed to evaluate the three different formulas of Sucrosin technology in increasing sugarcane growth and productivity. The study was conducted on PC sugarcane at RNI Plantation, Majalengka, West Java, using the Bululawang 1303 variety. The research design was a completely randomized design with four treatments consisting of Sucrosin technology existing (Se), formula 1 (S1), and formula 2 (S2) and control. The application of formula 2 Sucrosin technology (S2) could significantly increase the height and diameter of sugarcane stalks at 6 and 9 MAP. Sugarcane production in Se, S1, and S2 treatments increased by 22.49% (112.71 ton ha-1), 20.47% (110.85 ton ha-1), and 16.45% (107.15 ton ha-1) compared to controls (92.02 ton ha-1), respectively. These results show that Sucrosin Se technology produces the highest production. Nevertheless, from a technical perspective for implementation in the field, the most straightforward Sucrosin technology is S2. However, further research needs to be conducted in the broader area across different agro-ecologies to confirm the current findings.
蔗糖素技术已经在超过1万公顷的甘蔗种植面积上进行了测试，结果是生产力的提高变化很大。然而，在大范围实施时，实现标准的操作程序是复杂的，因此需要在产品配方和应用技术上进行简化。本研究旨在评价三种不同配方的蔗糖素技术对甘蔗生长和生产力的促进作用。本研究在西爪哇Majalengka RNI种植园进行，选用Bululawang 1303品种。研究设计为完全随机设计，采用现有Sucrosin技术(Se)、公式1 (S1)、公式2 (S2)和对照4个处理。施用2式蔗糖素技术(S2)可显著提高6、9 MAP甘蔗茎秆的高度和直径。与对照(92.02 t ha-1)相比，Se、S1和S2处理的甘蔗产量分别增加22.49% (112.71 t ha-1)、20.47% (110.85 t ha-1)和16.45% (107.15 t ha-1)。结果表明，蔗糖素硒技术的产量最高。然而，从技术角度来看，在现场实施，最直接的Sucrosin技术是S2。然而，进一步的研究需要在更广泛的领域进行，跨越不同的农业生态学，以证实目前的发现。
{"title":"Pengaruh tiga jenis formula teknologi Sucrosin terhadap pertumbuhan dan produktivitas tebu (Saccharum officinarum L.)","authors":"Muhammad Abdul Aziz, Ciptadi Ahmad Yusup, Siswanto Siswanto, Djoko Santoso, Priyono Priyono, Happy Widiastuti","doi":"10.22302/e-journalmenaraperkebunan.v91i2.545","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.545","url":null,"abstract":"Sucrosin technology, have been tested in more than 10,000 ha of sugarcane planting area, resulted in highly variable increases in productivity. However, fulfilling standard operational procedures when implemented on a wide scale is complicated, so it needs to be simplified regarding product formulation and application techniques. This study aimed to evaluate the three different formulas of Sucrosin technology in increasing sugarcane growth and productivity. The study was conducted on PC sugarcane at RNI Plantation, Majalengka, West Java, using the Bululawang 1303 variety. The research design was a completely randomized design with four treatments consisting of Sucrosin technology existing (Se), formula 1 (S1), and formula 2 (S2) and control. The application of formula 2 Sucrosin technology (S2) could significantly increase the height and diameter of sugarcane stalks at 6 and 9 MAP. Sugarcane production in Se, S1, and S2 treatments increased by 22.49% (112.71 ton ha-1), 20.47% (110.85 ton ha-1), and 16.45% (107.15 ton ha-1) compared to controls (92.02 ton ha-1), respectively. These results show that Sucrosin Se technology produces the highest production. Nevertheless, from a technical perspective for implementation in the field, the most straightforward Sucrosin technology is S2. However, further research needs to be conducted in the broader area across different agro-ecologies to confirm the current findings.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"114 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134907916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cellulose extraction typically begins with a delignification reaction using conventional methods, namely alkaline treatment. So far, the delignification process using alkaline treatment requires quite a long time, which is over 6 hours of the cooking process, so the cost to produce cellulose is quite large. The delignification approach using a variety of solvents is needed to answer the current problem, which is to shorten the reaction time. In this study, two types of solvents were carried out for the delignification process of EFB, namely NaOH and H2O2, while the time used for the delignification process was 15 minutes. Parameters of research observations included levels of lignin, cellulose, and hemicellulose.Extraction of cellulose from EFB using H2O2 and NaOH produced cellulose content of 52.76% and 66.46%, respectively. However, based on visual results of treatment using NaOH are still brown in color which indicates that lignin is still contained in EFB. The Fourier Transform Infra Red (FTIR)and X-Ray Diffraction (XRD) characterization results before and after delignified EFB showed that a peak of 3301.75 cm−1 dominated the functional group that show cellulose, with the highest crystallinity index of 12.43% in the H2O2 treatment. The results of Thermogravimetry Analysis (TGA)and Scanning Electron Microscopy (SEM) analyses showed that EFB before and after treatment were different, which can be observed from the loss of lignin structure and TGA values which began to degrade at 351.78°C. Therefore, based on this research the best solvent for fast delignification of EFB was H2O2 (only need 15 min).
{"title":"Characterization of cellulose from oil palm empty fruit bunches by fast delignification process with different solvents","authors":"Firda Dimawarnita, Yora Faramitha, Haryo Tejo Prakoso, Indah Puspitasari, Doni Nugroho Kalbuadi, Dedy Prasetyo","doi":"10.22302/mp.v91i2.542","DOIUrl":"https://doi.org/10.22302/mp.v91i2.542","url":null,"abstract":"Cellulose extraction typically begins with a delignification reaction using conventional methods, namely alkaline treatment. So far, the delignification process using alkaline treatment requires quite a long time, which is over 6 hours of the cooking process, so the cost to produce cellulose is quite large. The delignification approach using a variety of solvents is needed to answer the current problem, which is to shorten the reaction time. In this study, two types of solvents were carried out for the delignification process of EFB, namely NaOH and H2O2, while the time used for the delignification process was 15 minutes. Parameters of research observations included levels of lignin, cellulose, and hemicellulose.Extraction of cellulose from EFB using H2O2 and NaOH produced cellulose content of 52.76% and 66.46%, respectively. However, based on visual results of treatment using NaOH are still brown in color which indicates that lignin is still contained in EFB. The Fourier Transform Infra Red (FTIR)and X-Ray Diffraction (XRD) characterization results before and after delignified EFB showed that a peak of 3301.75 cm−1 dominated the functional group that show cellulose, with the highest crystallinity index of 12.43% in the H2O2 treatment. The results of Thermogravimetry Analysis (TGA)and Scanning Electron Microscopy (SEM) analyses showed that EFB before and after treatment were different, which can be observed from the loss of lignin structure and TGA values which began to degrade at 351.78°C. Therefore, based on this research the best solvent for fast delignification of EFB was H2O2 (only need 15 min).","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135321772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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