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Toxoplasma gondii and Neospora caninum antibody seroprevalence and risk factors among dogs treated at Public Veterinary Hospitals in São Paulo, Brazil 巴西圣保罗公立兽医医院治疗犬的刚地弓形虫和犬新孢子虫抗体血清阳性率及危险因素
4区 农林科学 Q4 PARASITOLOGY Pub Date : 2023-01-01 DOI: 10.1590/s1984-29612023058
Elidia Zotelli dos Santos, Herbert Souza Soares, Stephanie Rodrigues dos Santos, Jonas Moraes Filho, Hilda Fátima de Jesus Pena, Marcos Amaku, Solange Maria Gennari
Abstract Dogs can be infected by Toxoplasma gondii and Neospora caninum, for which they function, respectively, as intermediate, and definitive hosts. In the present study seroprevalence against T. gondii and N. caninum antibodies, were determined by indirect fluorescent antibody test (cut off of 16 and 50, respectively), in dogs that were treated at public veterinary hospitals in the metropolitan region of São Paulo and risk factors were identified. Out of the 1,194 samples 125 (10.5%; 95% CI: 8.8-12.3%) were positive for T. gondii and 9 (0.75%, 95% CI: 0.34-1.4%) for N. caninum. For T. gondii, statistical differences were observed between the proportions of positive dogs and different zones of the municipality (p = 0.025), and age (p = 0.02), higher among older dogs. The keepers were invited to answer an epidemiological questionnaire to analyze risk factors, and 471 (39.4%) agreed to be interviewed, and among their dogs 65 (13.8%) were T. gondii seropositive. Age group above 8 years (OR = 3.63; 95% CI: 1.08-12.23) was a risk factor and having a defined breed (OR = 0.49; 95% CI: 0.25-0.96) was a protective factor for T. gondii infection. Because of the low number of dogs positive for N. caninum, risk factors for this coccidium were not determined.
狗可感染刚地弓形虫和犬新孢子虫,它们分别作为中间宿主和最终宿主。在本研究中,通过间接荧光抗体试验(分别切断16和50)测定了在圣保罗大都会区公立兽医医院接受治疗的狗对弓形虫和犬奈瑟虫抗体的血清阳性率,并确定了危险因素。在1194个样本中,125个(10.5%;95% CI: 8.8 ~ 12.3%)弓形虫阳性,9例(0.75%,95% CI: 0.34 ~ 1.4%)犬奈虫阳性。弓形虫阳性犬的比例在城市不同区域和年龄之间存在统计学差异(p = 0.025),年龄较大的犬阳性率更高(p = 0.02)。调查结果显示,共有471人(39.4%)同意接受调查,其中弓形虫血清阳性65人(13.8%)。8岁以上年龄组(OR = 3.63;95% CI: 1.08-12.23)是危险因素,有一个明确的品种(OR = 0.49;95% CI: 0.25 ~ 0.96)是弓形虫感染的保护因素。由于犬奈瑟菌呈阳性的犬只数量较少,因此未确定感染该球虫的危险因素。
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引用次数: 0
Toxoplasma gondii infection in pig intended for human consumption: seroprevalence, risk factors and influence of biosecurity measures 供人食用猪中刚地弓形虫感染:血清阳性率、危险因素和生物安全措施的影响
4区 农林科学 Q4 PARASITOLOGY Pub Date : 2023-01-01 DOI: 10.1590/s1984-29612023060
Agostinho Sergio Scofano, Igor Falco Arruda, Jessica Nogueira Teixeira, Nathalie Costa da Cunha, Elmiro Rosendo do Nascimento, Maria Regina Reis Amendoeira, Patrícia Riddell Millar
Abstract A serologic and epidemiologic study was carried out in order to determinate herd and animal seroprevalence and associated factors for Toxoplasma gondii in commercial pigs from Espírito Santo state, Brazil. Blood samples were collected from 416 pigs from 55 producer farms in 27 municipalities. An indirect immunofluorescent assay (IFA) was performed to estimate the seroprevalence of T. gondii and identify the associated risk factors using a questionnaire. The T. gondii antibody prevalence rate in commercial swine herds was 15.4% (64/416) using a cutoff of 1:64. The seropositivity for T. gondii was related to the presence of cats, water origin and age of swine in the increase of seroprevalence, and the existence of internal isolation fences and use of composting chambers as protective factors. To the best of our knowledge, this is the first study to report anti- T. gondii antibodies in the serum of pigs in the state of Espírito Santo, Brazil. This finding is important to public health because seropositive pigs can harbor tissue cysts in their meat, thereby representing a zoonotic risk for consumers of raw or undercooked porcine meat or its products.
摘要为了确定巴西圣州Espírito商品猪群和动物弓形虫的血清阳性率及其相关因素,进行了血清学和流行病学研究。从27个城市55个养殖场的416头猪身上采集了血液样本。采用间接免疫荧光法(IFA)估计弓形虫的血清阳性率,并通过问卷调查确定相关危险因素。商品猪群中弓形虫抗体流行率为15.4%(64/416),截止值为1:64。弓形虫血清阳性率的升高与猫的存在、水源和猪的年龄有关,与室内隔离围栏的存在和堆肥池的使用有关。据我们所知,这是巴西圣Espírito州猪血清中首次报道抗弓形虫抗体的研究。这一发现对公共卫生很重要,因为血清呈阳性的猪可能在其肉中含有组织囊肿,因此对生猪肉或未煮熟猪肉或其产品的消费者来说,这代表着人畜共患风险。
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引用次数: 0
Morphological identification of Skrjabinisakis Mozgovoi, 1951 (Nematoda: Anisakidae) in Kogia sima (Cetacea: Kogiidae) from Brazilian waters 巴西水域 Kogia sima (Cetacea: Kogiidae) 中 Skrjabinisakis Mozgovoi, 1951 (Nematoda: Anisakidae) 的形态鉴定
4区 农林科学 Q4 PARASITOLOGY Pub Date : 2023-01-01 DOI: 10.1590/s1984-29612023064
Raul Henrique da Silva Pinheiro, Andréa Magalhães Bezerra, Elane Guerreiro Giese
Abstract New morphological, morphometric and scanning electron microscopy data of a nematode of the family Anisakidae, recovered from a specimen of Kogia sima, a cetacean that died off the northern coast of Brazil, are presented in this paper. Morphological features such as the violin-shaped ventricle and short and equal spicules, as well as the distribution of post-cloacal papillae and specificity for the definitive host (Kogiidae cetaceans) demonstrate similarity to Skrjabinisakis paggiae. This research records Kogia sima and S. paggiae on the estuarine coast of Pará, northern Brazil.
摘要:本文报道了从巴西北部海岸死亡的鲸类动物Kogia sima标本中发现的一种新形态、形态计量学和扫描电镜数据。其小提琴状脑室、短而相等的针状体、囊后乳头的分布以及最终寄主(鲸科)的特异性等形态学特征均与paggiae Skrjabinisakis相似。这项研究记录了巴西北部帕尔河口海岸的Kogia sima和S. paggiae。
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引用次数: 0
Occurrence of gastrointestinal parasites in dogs and cats domiciliated in Santos, SP, Brazil 巴西SP桑托斯市犬猫胃肠道寄生虫的发生情况
IF 1.3 4区 农林科学 Q4 PARASITOLOGY Pub Date : 2021-10-22 DOI: 10.1590/S1984-29612021080
Nathalia Delucinhore Lima, D. C. Raimundo, V. D. Souza, Juliana Martins Aguiar
Abstract Helminths and protozoa are major causes of diseases in domestic animals, and many can also cause infections in humans. Knowledge of intestinal parasitoses affecting domestic animals is important for the implementation of appropriate preventive measures. The objective of this study was to evaluate the occurrence of gastrointestinal parasites in fecal samples of dogs and cats attended at the Veterinary Hospital of the Metropolitan University of Santos, SP, Brazil. We also attempted to determine whether such infection was associated with sex, age, or the presence of diarrhea. We analyzed 100 fecal samples: 85 from dogs and 15 from cats. Among the dogs, 31.8% of the samples were positive, and to 40.0% among the cats. Infection was not associated with sex or age. However, among the dogs, parasitism showed a significant association with the presence of diarrhea (P = 0.013). The helminths Ancylostoma spp. and the protozoa Giardia duodenalis were the most frequent parasites in this research. Although they present unknown species and assemblages, they are parasites with a zoonotic potential of great importance in public health. Therefore, it is essential that pets are properly diagnosed and treated against gastrointestinal parasitic infection to prevent the spread of diseases.
蠕虫和原生动物是家畜疾病的主要原因,其中许多也可引起人类感染。了解影响家畜的肠道寄生虫对实施适当的预防措施很重要。本研究的目的是评估在巴西桑托斯城市大学兽医医院就诊的狗和猫的粪便样本中胃肠道寄生虫的发生率。我们还试图确定这种感染是否与性别、年龄或腹泻的存在有关。我们分析了100份粪便样本:85份来自狗,15份来自猫。在狗中,31.8%的样本呈阳性,在猫中为40.0%。感染与性别和年龄无关。然而,在狗中,寄生虫与腹泻的存在显著相关(P = 0.013)。本研究中最常见的寄生虫是钩虫和十二指肠贾第鞭毛虫。虽然它们存在未知的种类和组合,但它们是具有人畜共患潜力的寄生虫,对公共卫生具有重要意义。因此,对宠物进行正确的胃肠道寄生虫感染诊断和治疗,以防止疾病传播是至关重要的。
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引用次数: 4
Chemical constituents of Calotropis procera latex and ultrastructural effects on Haemonchus contortus 大角角蛾乳胶的化学成分及对弯血螨的超微结构影响
IF 1.3 4区 农林科学 Q4 PARASITOLOGY Pub Date : 2020-01-01 DOI: 10.1590/s1984-29612020045
G. S. Cavalcante, S. Morais, W. André, J. V. Araújo-Filho, C. Muniz, L. O. Rocha, W. Ribeiro, Ana Livya Moreira Rodrigues, L. Oliveira, C. Bevilaqua, M. Ramos
Abstract This study aimed to evaluate the anthelmintic and ultrastructural effects of Calotropis procera latex on Haemonchus contortus. C. procera latex was twice centrifuged at 10,000×g and dialyzed to obtain a fraction rich in proteins, named LP (latex protein), and at 3,000 rpm to obtain a fraction rich in secondary metabolites, named LNP (latex non-protein). Specimens of H. contortus exposed to LNP, LP and PBS in the Adult Worm Motility Test (AWMT) were submitted to scanning (SEM) and transmission (TEM) electron microscopy to verify changes in their ultrastructure. Phytochemical tests in the LNP indicated the presence of phenols, steroids, alkaloids and cardenolides. High-Performance Liquid Chromatography (HPLC) characterized the presence of the compounds gallic acid and quercetin in the LNP. The protein content in the LP was 43.1 ± 1.1 mg/mL and 7.7 ± 0.3 mg/mL in LNP. In AWMT, LNP and LP inhibited the motility of 100% of the nematodes, with LNP being more effective than LP and ivermectin more effective than both (p <0.05). Cuticle changes were observed by SEM and TEM in nematodes treated with LP and LNP. Calotropis procera latex has anthelmintic effects against H. contortus, causing damage to its cuticle and other alterations in its ultrastructure.
摘要本研究旨在探讨大角角蛾乳剂对弯血螨的驱虫作用和超微结构作用。在10,000×g进行两次离心,透析得到富含蛋白质的部分,命名为LP(乳胶蛋白),在3000 rpm下得到富含次级代谢物的部分,命名为LNP(乳胶非蛋白)。采用扫描电镜(SEM)和透射电镜(TEM)观察在LNP、LP和PBS作用下的H. contortus成虫运动试验(AWMT)的超微结构变化。LNP中的植物化学测试表明存在酚类,类固醇,生物碱和核桃苷。高效液相色谱(HPLC)表征了LNP中未食子酸和槲皮素的存在。LP蛋白含量为43.1±1.1 mg/mL, LNP蛋白含量为7.7±0.3 mg/mL。在AWMT中,LNP和LP对线虫运动的抑制率均为100%,LNP的抑制率高于LP,伊维菌素的抑制率高于两者(p <0.05)。用扫描电镜和透射电镜观察了LP和LNP处理后线虫表皮的变化。胼胝体胶乳对麻蝇有驱虫作用,可引起麻蝇角质层损伤和超微结构改变。
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引用次数: 11
Nematoides anisaquídeos e cestoides Trypanorhyncha de importância em saúde pública em Aluterus monoceros (Linnaeus, 1758) no Estado do Rio de Janeiro, Brasil 巴西里约热内卢州Aluterus monoceros (Linnaeus, 1758)的异线虫和锥虫对公共卫生具有重要意义
IF 1.3 4区 农林科学 Q4 PARASITOLOGY Pub Date : 2010-04-01 DOI: 10.1590/S1984-29612010000200005
F. D. J. E. Dias, S. C. D. S. Clemente, Marcelo Knoff
One hundred specimens of unicorn leatherjacket, Aluterus monoceros purchased from markets of municipalities of Niteroi and Rio de Janeiro from May to August 2006. The fishes were measured, necropsied, fileted and analysed their organs. Sixteen fishes were parasitized by nematode Anisakidae: Anisakis spp. and Contracaecum sp. with respectively, 1 and 16% of prevalence, 2 and 3.31 of mean intensity, and 0.02 and 0.53 of mean abundance. Two larvae of Anisakis sp. were found in mesentery of one fish and Contracaecum sp. was found in liver and mesentery with 1 to 9 specimens of range of infection. Fifty-one fishes were parasitized on the liver and mesentery by metacestodes of Trypanorhyncha. The collected species were Floriceps saccatus and Callitetrarhynchus speciosus with respectively, 45 and 6% of prevalence, 3.17 and 2.83 of mean intensity, and 1.43 and 0.06 of mean abundance, the range of infection by F. saccatus was 1 to 20 and by C. speciosus was 1 to 5. Anisakis sp. and these two species of Trypanothyncha were reported in A. monoceros for the first time.
2006年5月至8月在尼泰罗伊市和里约热内卢市市场购买的独角兽皮夹克、独角兽长角兽标本100件。对这些鱼进行了测量、解剖、切片和器官分析。16条鱼被异丝线虫寄生:异丝线虫和收缩线虫,患病率分别为1%和16%,平均强度分别为2%和3.31%,平均丰度分别为0.02和0.53。在1条鱼的肠系膜内发现异尖线虫幼虫2只,在肝脏和肠系膜内发现收缩绦虫1 ~ 9只。51条鱼的肝脏和肠系膜被锥虫寄生。采集到的品种为花蜱(Floriceps sacaccatus)和斑喙纹蜱(calitetrarhynchus speciosus),其感染率分别为45%和6%,平均强度分别为3.17和2.83,平均丰度分别为1.43和0.06,感染范围为1 ~ 20,感染范围为1 ~ 5。异尖线虫和这两种锥虫均为首次在单角田鼠中报道。
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引用次数: 25
Utilização do método Famacha no diagnóstico clínico individual de haemoncose em ovinos no Sudoeste do Estado de Minas Gerais 法马查法在米纳斯吉拉斯州西南部绵羊血吸虫病个体临床诊断中的应用
IF 1.3 4区 农林科学 Q4 PARASITOLOGY Pub Date : 2010-01-01 DOI: 10.1590/S1984-29612010000100014
Diana Cuglovici Abrão, S. Abrão, C. H. C. Viana, C. R. Valle
The aim of this study was to evaluate the efficacy of Famacha method in individual clinical diagnosis of haemonchosis in sheep from the south-west of Minas Gerais. Fifty crossbred Santa Ines ewes were evaluated during 5 months as for conjunctival mucous colour (Famacha method), Packed Cell Volume (PCV), Faecal Egg Counts (FEC), body score and clinic alterations. The score Famacha observed was from 1 to 3, the body score from 2 to 5, the PCV from 20 to 47%, and FEC from 0 to 7,750. The correlations between the parameters evaluated, except Famacha with body score, were highly significant (P < 0.0001). The main genus identified in the coprocultures was Haemonchus (87 - 100% of total larvae, ± 93.5%). We observed reduction of 87.33% in the use of anthelmintics on the property. It was concluded that the Famacha method, associated with other diagnostic methods, is a useful tool to detect hemonchosis in sheep.
本研究的目的是评估Famacha方法在米纳斯吉拉斯州西南部绵羊血液病个体临床诊断中的有效性。在5个月内对50只杂交圣伊内斯母羊进行结膜黏液颜色(Famacha法)、堆积细胞体积(PCV)、粪卵计数(FEC)、身体评分和临床变化的评估。Famacha的评分范围为1 ~ 3分,体分范围为2 ~ 5分,PCV范围为20 ~ 47%,FEC范围为0 ~ 7750分。除Famacha与体评分外,各评价参数间的相关性均极显著(P < 0.0001)。共培养的主要属为血螨,占总幼虫的87 ~ 100%,±93.5%;我们观察到该物业的驱虫药使用减少了87.33%。结果表明,Famacha法与其他诊断方法相结合,是检测绵羊血病的有效方法。
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引用次数: 20
Toxoplasma gondii: diagnóstico da infecção experimental e natural em pombos (Columba livia) por técnicas sorológicas, biológicas e moleculares. 刚地弓形虫:用血清学、生物学和分子技术诊断鸽子(Columba livia)的实验性和自然感染。
IF 1.3 4区 农林科学 Q4 PARASITOLOGY Pub Date : 2009-12-15 DOI: 10.11606/D.42.2009.TDE-01022010-094111
Fernanda Sartori Lima de Godoi
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引用次数: 2
Parasitas zoonóticos em fezes de cães em praças públicas do município de Itabuna, Bahia, Brasil 巴西巴伊亚州伊塔布纳市公共广场狗粪便中的人畜共患病寄生虫
IF 1.3 4区 农林科学 Q4 PARASITOLOGY Pub Date : 2008-10-01 DOI: 10.1590/S1984-29612008000400007
Pedro Paulo da Costa Alves Filho, L. Barros, J. O. Campos, Vania Braga, I. Cazorla, George Rêgo Albuquerque, S. Carvalho
The contamination of public squares by zoonotic potential parasites was evaluated at the urban areas in the municipality of Itabuna Brasil. For such, 119 fecal samples of dogs were collected at 10 public squares. After that, these feces samples were transported to the Parasitology Laboratory in the UESC and they were analyzed by Mariano and Carvalho's method. Of the total analized samples, 56.3% show some parasitic evolutive form. Ancylostoma sp. was the most frequently (47.9%), followed by Strongyloides stercortalis (6.7%), Toxocara canis and Trichuris vulpis eggs (4.2%) each, Endolimax nana cysts (2.5%), and Giardia intestinalis and Entamoeba coli cysts (0.8%) each.
在巴西伊塔布纳市市区对公共广场的潜在人畜共患寄生虫污染进行了评估。为此,在10个公共广场收集了119份狗的粪便样本。之后,这些粪便样本被运送到UESC的寄生虫学实验室,并采用Mariano和Carvalho的方法进行分析。在全部分析样本中,56.3%的样本显示出某种寄生进化形式。最常见的是钩虫(47.9%),其次是腹圆线虫(6.7%)、犬弓形虫和毛缕虫卵(4.2%)、内巨鞭毛虫囊(2.5%)、肠贾第虫囊和大肠内阿米巴囊(0.8%)。
{"title":"Parasitas zoonóticos em fezes de cães em praças públicas do município de Itabuna, Bahia, Brasil","authors":"Pedro Paulo da Costa Alves Filho, L. Barros, J. O. Campos, Vania Braga, I. Cazorla, George Rêgo Albuquerque, S. Carvalho","doi":"10.1590/S1984-29612008000400007","DOIUrl":"https://doi.org/10.1590/S1984-29612008000400007","url":null,"abstract":"The contamination of public squares by zoonotic potential parasites was evaluated at the urban areas in the municipality of Itabuna Brasil. For such, 119 fecal samples of dogs were collected at 10 public squares. After that, these feces samples were transported to the Parasitology Laboratory in the UESC and they were analyzed by Mariano and Carvalho's method. Of the total analized samples, 56.3% show some parasitic evolutive form. Ancylostoma sp. was the most frequently (47.9%), followed by Strongyloides stercortalis (6.7%), Toxocara canis and Trichuris vulpis eggs (4.2%) each, Endolimax nana cysts (2.5%), and Giardia intestinalis and Entamoeba coli cysts (0.8%) each.","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"93 1","pages":"206-209"},"PeriodicalIF":1.3,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80270618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
期刊
Revista Brasileira De Parasitologia Veterinaria
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