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Comparative Assessment of Antibacterial Activity of Commercially Available Antibiotics and Cinnamomum Verum (Cinnamon) Against Salmonella Typhi and Para Typhi Infections 市售抗生素与肉桂对伤寒沙门氏菌和副伤寒杆菌感染的抗菌活性比较评估
Pub Date : 2024-06-03 DOI: 10.57038/usjas.v8i01.6960
Muhammad Asim, Abdus Samad, Shafi Ullah, Shawkat Ali, Maria Karim, Zubaida Saad, Saira Gul, Rubina Shakil
Typhoid fever is a life threating disease occurs due to ingestion of contaminated food products. Salmonella spp. are highly pathogenic in nature because they have the ability to produce endotoxins within a body. The present research study was designed to carry out comparative assessment of antibacterial activity of commercially available antibiotics and Cinnamomum verum extracts prepared in different solvents against S. typhi and paratyphi infections. In the current study, a total of 500 samples of blood were collected from typhoid patients in pre-sterilized bottles. After that serological tests were performed. Widal test was used for typhoid diagnosis and gave highest efficacy (52.6%) in the diagnosis of salmonellosis. Furthermore, Widal positive samples were investigated for the presence of pathogenic bacteria through conventional culturing technique and two major bacterial species i.e. S. typhi (n = 85) and S. paratyphi (n = 25) were identified. In the current study, Further more, antibiotic sensitivity assay was performed according to Kirby-Bauer disc diffusion method and it was observed that all S. typhi isolates were sensitive to Sulbactam (38±2.23 mm) followed by Ceftazidime (37±2.8 mm), Tazobactam (36±2.5 mm), Amoxicillin (35±3.33 mm), Amikacin (29±1.7 mm), Gentamycin (28±1.8 mm), Cephradine (26±2.9 mm) and Meropenem (18±3.1 mm). On the other hand, S. typhi showed resistance to Ciprofloxacin (1.5±0.8 mm) and Ofloxacin (1±0.77 mm). Additionally, it was also observed that all S. paratyphi showed highest sensitivity to Ciprofloxacin (34±0.87 mm), Ofloxacin (30±1.67 mm), Ceftazidime (28±3.1 mm), Tazobactam (28±2.4 mm) and Sulbactam (28±1.14 mm) while showed resistance to Gentamycin (12±2.8 mm). Furthermore, it was suggested that proper care and preventive measures should be taken for patients with bacterial infections and also  antibiotics selection must be after performing culture sensitivity tests. Beside this, different plant extracts should be recommended as alternative for the treatment of typhoid infection and by doing this, chances of antibiotic resistance might be reduced up to greater extent.
伤寒是一种因摄入受污染食品而导致的危及生命的疾病。沙门氏菌属具有很强的致病性,因为它们能够在人体内产生内毒素。本研究旨在比较评估市售抗生素和肉桂提取物在不同溶剂中对伤寒杆菌和副伤寒杆菌感染的抗菌活性。在本次研究中,共从伤寒患者身上采集了 500 份血液样本,并将其装入预先消毒的瓶子中。然后进行血清学检测。伤寒诊断采用威达试验,该试验对沙门氏菌病的诊断有效率最高(52.6%)。此外,还通过传统培养技术对威达试验阳性样本中的病原菌进行了调查,并确定了两种主要的细菌种类,即伤寒杆菌(85 个)和副伤寒杆菌(25 个)。在本研究中,还根据柯比鲍尔盘扩散法进行了抗生素敏感性检测,结果发现所有伤寒杆菌分离物对舒巴坦敏感(38±2.23 mm),其次是头孢唑肟(37±2.8 mm)、他唑巴坦(36±2.5 mm)、阿莫西林(35±3.33 mm)、阿米卡星(29±1.7 mm)、庆大霉素(28±1.8 mm)、头孢拉定(26±2.9 mm)和美罗培南(18±3.1 mm)。另一方面,伤寒杆菌对环丙沙星(1.5±0.8 毫米)和氧氟沙星(1±0.77 毫米)表现出耐药性。此外,还观察到所有副伤寒杆菌对环丙沙星(34±0.87 mm)、氧氟沙星(30±1.67 mm)、头孢唑肟(28±3.1 mm)、他唑巴坦(28±2.4 mm)和舒巴坦(28±1.14 mm)均表现出最高的敏感性,而对庆大霉素(12±2.8 mm)则表现出耐药性。此外,建议对细菌感染患者采取适当的护理和预防措施,并在进行培养药敏试验后选择抗生素。此外,应推荐使用不同的植物提取物作为治疗伤寒感染的替代品,这样做可以在更大程度上减少抗生素耐药性的产生。
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引用次数: 0
Impact of Zinc Supplementation on Tharparkar Bull Semen Quality: Comparative Study of Commercial and Lab Extenders 补锌对塔帕卡尔公牛精液质量的影响:商用和实验室添加剂的比较研究
Pub Date : 2024-06-03 DOI: 10.57038/usjas.v8i01.6955
Abdul Hameed, A. Channo, A. Kaka, Habibullah Janyaro
This study was aimed to evaluate the impact of zinc supplementation on chilled and frozen thawed quality parameters of Tharparkar bull semen by comparing the use of BULLXcell® (Commercial) and tris-based egg yolk (Lab based) extenders impact on semen quality parameters. The research was conducted at the Department of Animal Reproduction, Sindh Agriculture University Tandojam. Total 44 semen ejaculates were collected from four Tharparkar Bulls (A, B, C, and D) aged 4-5 years using an artificial vagina. Macroscopic and Microscopic parameters i.e. color: visual observation, volume: graduated tube, pH: digital pH meter, Progressive Motility (20x magnification), morphology and viability (eosin-nigrosin stain), concentration (haemocytometer method), and membrane integrity (HOST solution), were evaluated. Semen samples with progressive motility, morphology, viability, and membrane integrity exceeding 70% were pooled and processed. The pooled semen samples were further extended by pouring into four groups [A: BullXcell® control, B: BullXcell®+Zinc (0.2 mg/100 ml), C: Tris+Control, D: Tris+Zinc (0.2 mg/100 ml)]. Chilled assessment of progressive motility, morphology, viability, and membrane integrity revealed that Group B (78.65±0.85, 85.23±0.81, 81.22±1.17, 80.51±0.73) (P<0.05) demonstrated improved results compared to Group D (73.82±0.91, 81.66±1.14, 76.51±1.22, 76.27±1.07). Similarly, post-thawed assessment of these parameters indicated superior results for Group B (57.25±0.82, 75.17±1.02, 70.22±1.34, 63.55±1.39) (P<0.05) compared to Group D (47.35±0.66, 63.24±1.47, 65.05±1.63, 57.14±1.02) followed A and C. Based on the findings, it was concluded that Group B [BullXcell®+Zinc (0.2 mg/100 ml)] significantly improved both Chilled and post thawed quality parameters of Tharparkar cattle bull semen and the inclusion of Zinc in extenders is recommended as it improved viability of spermatozoa.
本研究旨在通过比较使用 BULLXcell®(商用)和三聚氰胺蛋黄(实验室用)扩增剂对精液质量参数的影响,评估补锌对冷藏和冷冻解冻的塔帕卡尔公牛精液质量参数的影响。这项研究在信德农业大学坦多贾姆分校动物繁殖系进行。使用人工阴道从四头年龄为 4-5 岁的 Tharparkar 公牛(A、B、C 和 D)共收集了 44 滴精液。对精液的宏观和显微参数进行了评估,即:颜色:肉眼观察;体积:刻度管;pH 值:数字 pH 计;渐进运动性(20 倍放大镜);形态和活力(伊红-曙红染色法);浓度(血球计数法);膜完整性(HOST 溶液)。将运动性、形态、存活率和膜完整性超过 70% 的精液样本集中起来进行处理。将汇集的精液样本进一步扩大,分成四组[A:BullXcell® 对照组;B:BullXcell®+锌(0.2 毫克/100 毫升);C:Tris+对照组;D:Tris+锌(0.2 毫克/100 毫升)]。冷冻评估进行性运动、形态、存活率和膜完整性显示,B 组(78.65±0.85、85.23±0.81、81.22±1.17、80.51±0.73)(P<0.05)的结果优于 D 组(73.82±0.91、81.66±1.14、76.51±1.22、76.27±1.07)。同样,与 D 组(47.35±0.66、63.24±1.47、65.05±1.63、57.14±1.02)相比,B 组(57.25±0.82、75.17±1.02、70.22±1.34、63.55±1.39)(P<0.05)的解冻后评估结果优于 A 组和 C 组(57.25±0.82、75.17±1.02、70.22±1.34、63.55±1.39)(P<0.05)。根据研究结果,B 组[BullXcell®+锌(0.2 毫克/100 毫升)]明显改善了 Tharparkar 公牛精液的冷藏和解冻后质量指标,建议在扩展剂中加入锌,因为锌能提高精子的活力。
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引用次数: 0
Amazon Rainforest Wildfires: Causes and Impact on Mammalian and Avian Diversity 亚马逊雨林野火:原因及其对哺乳动物和鸟类多样性的影响
Pub Date : 2024-06-03 DOI: 10.57038/usjas.v8i01.6962
Wajeeha Tanveer, Sajida Mushtaq, Nimra Ijaz, Moazama Batool, S. Maalik
Amazon rainforest is having huge role in the regulation of the environment as it provides massive amount of oxygen to the Earth and is one of the most important carbon sinks. World’s most important plants and animal species are present in the Amazon rainforests. The Amazon rainforest wildfires especially wildfires of 2019 are one of the major global issues. There can be natural and human activities which can cause wildfires in Amazon Forest. The impacts due to wildfires in Amazon rainforest includes conversion of Amazon Forest into white savannas, threats to human security, negative effects on health, decrease in rainfall, effects on biodiversity, biomass burning aerosols, effects on ecosystem, increase in carbon emission, greenhouse effect, global warming and impact on environment and climatic feedback. In the end, some suggestions and strategies are given for the control of wildfires in the Amazon rainforest.
亚马逊雨林在调节环境方面发挥着巨大作用,因为它为地球提供了大量氧气,也是最重要的碳汇之一。亚马逊雨林中有世界上最重要的植物和动物物种。亚马逊雨林野火,尤其是 2019 年的野火,是全球重大问题之一。自然和人类活动都可能导致亚马逊森林发生野火。亚马逊雨林野火造成的影响包括:亚马逊森林变成白色热带草原、威胁人类安全、对健康产生负面影响、降雨量减少、影响生物多样性、生物质燃烧产生气溶胶、影响生态系统、碳排放增加、温室效应、全球变暖以及对环境和气候反馈的影响。最后,提出了一些控制亚马逊雨林野火的建议和战略。
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引用次数: 0
Effect of Physiological State on Meat Quality: An Insight from Buffalo 生理状态对肉质的影响:水牛的启示
Pub Date : 2024-06-03 DOI: 10.57038/usjas.v8i01.6954
Syed Hamad, UL Hassan, Syed Murtaza, Hassan Andrabi, MUHAMMAD FAROOQ IQBAL, Mudussar Nawaz, Asghar Khan, Muhammad Yaqoob, Zulfiqar Ahmed, Z. Naseer
The present study aimed to observe the effect of buffalo's physiological stage (heifers, postpartum, dry) on the physiochemical and organoleptic characteristics of meat. The collected meat samples were analyzed for physiochemical parameters (pH, water holding capacity, cooking loss, and drip loss), and a pre-trained panel also evaluated the organoleptic attributes of fresh and cooked buffalo meat. The physiochemical properties showed that heifer meat was of better-quality regarding pH, water holding capacity, cooking loss, and drip loss than dry or postpartum buffalo meat. The heifer meat displayed better (P < 0.05) fresh meat odor, color, taste, and juiciness compared with the postpartum buffalo meat. The palatability of meat and mouth-filling properties was comparatively higher (P < 0.05) in heifer meat. Cooked meat heifer meat showed lower (P < 0.05) hardiness and cohesiveness of mass, along springiness, and ease of swallowing ness. High (P < 0.05) saliva production and less (P < 0.05) residual particle and tooth packing properties were observed (P < 0.05) in heifer meat. Heifer meat was highly (P < 0.05) acceptable due to better palatability and mouth coating basis. The overall meat-liking quality ratio was also greater (P < 0.05) for heifer meat than postpartum buffalo meat. It is concluded that heifer meat was better in terms of physiological and organoleptic properties than dry and postpartum buffalo meat.
本研究旨在观察水牛的生理阶段(小母牛、产后水牛、干水牛)对肉的理化和感官特性的影响。研究人员对采集的肉样进行了理化参数(pH 值、持水量、蒸煮损失和滴水损失)分析,并由一个经过预先培训的小组对新鲜和蒸煮水牛肉的感官特性进行了评估。理化特性表明,小母牛肉在 pH 值、持水能力、蒸煮损失和滴水损失方面的质量优于干水牛肉或产后水牛肉。与产后水牛肉相比,小母牛肉的鲜肉气味、颜色、口感和多汁性更好(P < 0.05)。小母牛肉的适口性和口腔饱满度相对更高(P < 0.05)。煮熟后的小母牛肉的硬度和质量凝聚性、弹牙性和易吞咽性较低 (P < 0.05)。小母牛的唾液分泌量较高(P < 0.05),残留颗粒和牙齿包装性能较低(P < 0.05)(P < 0.05)。小母牛肉的适口性和口腔包裹性较好,因此可接受性较高 (P < 0.05)。与产后水牛肉相比,小母牛肉的总体肉质喜欢率也更高(P < 0.05)。结论是,小母牛肉在生理和感官特性方面优于干水牛肉和产后水牛肉。
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引用次数: 0
Innovation in Vaccines for Infectious Diseases in Pets and Livestocks 宠物和家畜传染病疫苗的创新
Pub Date : 2024-06-03 DOI: 10.57038/usjas.v8i01.6959
Adil Shahzad, Ammara Zulqarnain, Safdar Aman, Aman Ullah, Muhammad Junaid, Muhammad Huzaifah Khalid, Zaighum Ali, Irtaza Hussain, Muhammad Azwar, Aziz Chishti
This article summarises the current state of veterinary vaccines for animals and pets, as well as their significance and recent developments. The use of veterinary vaccines is vital to the food industry and public health because they protect the well-being and productivity of animals. This article emphasises the value of vaccinations in preventing and controlling zoonotic illnesses and other infectious diseases in animals. Preventing the spread of viral illnesses at the animal-human interaction is emphasised, and the article goes on to talk about the monetary effects of disease control efforts. The article goes on to discuss the several kinds of veterinary vaccines, such as the traditional live and inactivated virus vaccines, DNA vaccines, genetically engineered virus vaccines, molecularly specified subunit vaccines, and live viral vector vaccines. Additionally, it addresses vaccines against helminths and ectoparasites, allergies, cancer, protozoa, and bacteria in animals.
本文概述了动物和宠物兽用疫苗的现状、意义和最新发展。兽用疫苗的使用对食品工业和公共卫生至关重要,因为它们能保护动物的福利和生产力。本文强调了疫苗在预防和控制人畜共患病和其他动物传染病方面的价值。文章强调要防止病毒性疾病在人与动物之间的传播,接着谈到了疾病控制工作的经济效益。文章接着讨论了几种兽用疫苗,如传统的病毒活疫苗和灭活疫苗、DNA 疫苗、基因工程病毒疫苗、分子特定亚单位疫苗和活病毒载体疫苗。此外,文章还讨论了针对动物蠕虫和体外寄生虫、过敏症、癌症、原生动物和细菌的疫苗。
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引用次数: 0
Pest Management of Ficus Benjamina by Morphological Study of Lepidoptera Bombycidae 通过对鳞翅目蝠科昆虫的形态研究来管理本雅明榕的害虫
Pub Date : 2024-06-03 DOI: 10.57038/usjas.v8i01.6957
Jawad Ali Shah, Muhammad Irfan Bhatti, Muhammad Yasin, Ameer Hamza, Muhammad Akram, Muhammad Zeeshan, Hafiz ZILL-E-REHMAN, Faisal Saleem, Arbaz Khan, Gulfam Yousaf
This study investigates the developmental parameters of Trilocha varians, a species within the Lepidoptera order belonging to Bombycidae family, focusing on their interaction with the weeping fig, Ficus benjamina, a significant agricultural pest in Pakistan. The experiments were conducted at a controlled temperature of 26°C and a humidity level of 65%, simulating conditions relevant to the region. Observations revealed that female Trilocha varians laid 160-270 eggs in 2-6 layers on the dorsal part of the F. benjamina leaves. Interestingly, unmated females also deposited clusters of 15-183 eggs, which did not progress to larvae. The eggs turned black 24 hours before hatching. The incubation period for eggs on F. benjamina was found to be 3.46 ± 1.22 days. The survival rate of T. varians larvae was notably high, likely attributed to prolific reproduction. During the study period, five larval instars were identified, with the last two instars causing the most damage, particularly due to their resemblance to host parts such as bark. Notably, the ninth abdominal section of larvae featured thin, spongy horns. The duration of development for the first, second, third, fourth, and fifth larval instars was 2.44 ± 0.82, 3.56 ± 0.53, 3.72 ± 0.26, 4.54 ± 0.79, and 6.20 ± 0.78 days, respectively. The larvae exhibited boat-shaped and yellow silky characteristics. Upon reaching adulthood, the male and female Trilocha varians displayed distinctive features. The average lifespan for males was 6.23 ± 2.09 days, while females lived longer, with a mean longevity of 10.01 ± 0.99 days. Female forewings exhibited faint reddish-brown streaks on the dorsal side, and their thorax, head, and abdomen were darker reddish-brown compared to males. Adult hind wings were predominantly greyish with reddish-brown outer edges.This research provides valuable insights into the biology and behavior of Trilocha varians and their impact on F. benjamina. Understanding these developmental parameters is crucial for implementing effective pest management practices in agriculture, contributing to the sustainable protection of crops against this significant threat.
本研究调查了鳞翅目褐飞虱科的一个物种 Trilocha varians 的发育参数,重点是它们与垂枝无花果(Ficus benjamina)的相互作用,垂枝无花果是巴基斯坦的一种重要农业害虫。实验在温度控制在 26°C 和湿度 65% 的条件下进行,模拟了该地区的相关条件。观察发现,雌性 Trilocha varians 产卵 160-270 粒,分 2-6 层,产在 F. benjamina 叶子的背面。有趣的是,未交配的雌虫也会产下 15-183 粒卵块,但这些卵块并未发育成幼虫。卵在孵化前 24 小时变黑。发现卵在 F. benjamina 上的孵化期为 3.46 ± 1.22 天。T. varians 幼虫的存活率很高,这可能是由于其繁殖能力很强。在研究期间,共发现了 5 个幼虫龄期,其中后两个龄期造成的危害最大,特别是因为它们与寄主部位(如树皮)相似。值得注意的是,幼虫腹部第九节有细长的海绵状角。第一、第二、第三、第四和第五龄幼虫的发育时间分别为 2.44 ± 0.82、3.56 ± 0.53、3.72 ± 0.26、4.54 ± 0.79 和 6.20 ± 0.78 天。幼虫呈船形和黄色丝状。成年后,雌雄Trilocha varians表现出不同的特征。雄虫的平均寿命为 6.23 ± 2.09 天,而雌虫的寿命更长,平均寿命为 10.01 ± 0.99 天。雌性前翅背面有淡淡的红褐色条纹,胸部、头部和腹部与雄性相比呈深红褐色。这项研究为我们深入了解变角蝇的生物学和行为以及它们对苯加米纳蝇的影响提供了宝贵的资料。了解这些发育参数对于在农业中实施有效的害虫管理措施至关重要,有助于可持续地保护农作物免受这一重大威胁。
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引用次数: 0
Insect Pollinators of Buckwheat (Fagopyrum Esculentum) From District Ghanche, Gilgit Baltistan 吉尔吉特-巴尔蒂斯坦甘奇地区荞麦(Fagopyrum Esculentum)的昆虫传粉媒介
Pub Date : 2024-06-03 DOI: 10.57038/usjas.v8i01.6956
Riaz Hussain, Muhammad Ali, Zershina Maryam, Mehdi Hassan Shehbaz, SAKHAWAT ALI, MUHAMMAD HASSAN, Akmal Ali
Current study was initiated to investigate the insect pollinator fauna of Buckwheat crops at high elevations of District Ghanche, Gilgit Baltistan. In total, 507 specimens were collected during active flowering seasons of crop and yielding 17 species under 13 genera of 7 families belonging to three orders viz, Hymenoptera, Diptera and Lepidoptera. Order Hymenoptera includes family Formicidae, Apidae and Vespidae, Diptera includes Syrphidae, Calliphoridae and Muscidae while Pieridae belonged to order Lepidoptera. Most interesting information related to this research is that Pieris brassicae is considered as a pest, but it played major role in pollination of Buckwheat and not documented as a pest. No pest is recorded during whole research period.
本研究旨在调查吉尔吉特-巴尔蒂斯坦甘奇区高海拔地区荞麦作物的昆虫授粉动物群。在作物开花旺季共采集了 507 个标本,共发现 17 种昆虫,隶属于膜翅目、双翅目和鳞翅目的 7 科 13 属。膜翅目包括甲虫科、蚜虫科和蚜蝇科,双翅目包括蚜蝇科、茧蝇科和鹟科,而鳞翅目则有墩蝇科。与本研究有关的最有趣的信息是,黄刺茧蜂被认为是害虫,但它在荞麦授粉过程中发挥了重要作用,并未被记录为害虫。在整个研究期间没有记录到害虫。
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引用次数: 0
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University of Sindh Journal of Animal Sciences (USJAS)
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