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Cultivation options for indoor and outdoor growth of Chaetoceros gracilis with airlift photobioreactors 气升式光生物反应器在室内和室外培养毛羽毛藻的选择
IF 0.7 4区 综合性期刊 Q2 Multidisciplinary Pub Date : 2014-04-01 DOI: 10.14456/MIJST.2014.9
P. Pavasant, Watadta Ritcharoen, Puchong Sriouam, Piyanate Nakseedee, Patthama Sang, S. Powtongsook, Kunn Kungvansaichol
Various configurations and modes of airlift photobioreactors were examined in the cultivation of Chaetoceros gracilis. Internal loop and external loop airlifts were cultivated in a batch mode in a controlled indoor environment. The external loop system provided a better performance than the internal loop system due to better light exposure. A continuous operation was conducted in an internal loop airlift photobioreactors-inseries. This was designed to minimise the effect of light blocking due to overgrown cells as the high-density culture was, in this configuration, only limited to the last airlift column in the series. Outdoor large-scale operation was conducted in a flat-panel airlift photobioreactor. Due to uneven light availability, the outdoor culture could not perform as well as the indoor one in terms of growth rate. Among the four systems investigated, the continuous culture in airlift photobioreactors-in-series provided the best performance with the highest cell density of 12.12 × 10 cells mL. Cost analysis based on the maximum number of reactors that can be installed in one square metre indicates that the indoor system requires lowest operating cost per unit cultivation area, whereas the outdoor system provides highest profit as a result of the inherited large productivity.
研究了气升式光生物反应器在毛羽毛藻培养中的各种构型和模式。在受控的室内环境中分批培养内环和外环空运。由于更好的光照,外环系统比内环系统提供了更好的性能。在串联的内循环气升式光生物反应器中进行了连续操作。这样设计是为了尽量减少由于过度生长的细胞造成的光阻塞的影响,因为高密度培养在这种配置下,只局限于该系列的最后一个气举柱。在平板气升式光生物反应器中进行了室外大规模操作。由于光照不均匀,室外培养在生长速度上不如室内培养。在所调查的四种系统中,串联气升式光生物反应器连续培养的性能最好,细胞密度最高,为12.12 × 10个细胞mL。基于每平方米可安装的最大反应器数量的成本分析表明,室内系统每单位种植面积的运行成本最低,而室外系统由于继承了较大的生产率而提供了最高的利润。
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引用次数: 7
Statistical optimisation of culture conditions for biocellulose production by Komagataeibacter sp. PAP1 using soya bean whey 利用大豆乳清生产生物纤维素的Komagataeibacter sp. PAP1培养条件的统计优化
IF 0.7 4区 综合性期刊 Q2 Multidisciplinary Pub Date : 2014-01-01 DOI: 10.14456/MIJST.2014.1
A. Suwanposri, P. Yukphan, Yuzo Yamada, D. Ochaikul
A low-cost medium based on soya bean whey (SBW) was prepared as a by-product from agriculture and optimised for biocellulose (BC) production by Komagataeibacter sp. PAP1. The optimal conditions for BC production are: pH 6.21, 1.61% ethanol concentration (v/v) and 28.4 o C. The use of optimised medium based on SBW increased BC production 3.6 fold compared to standard Hestrin-Schramm (HS) medium. The BC film produced from the optimised medium was stronger and more impermeable to water vapour and oxygen compared to the one produced from the standard HS medium. These properties allow SBW film to be developed into eco-friendly food packaging for oxygen-sensitive products. Its high water absorption capacity allows its use in biomedical application such as wound dressing. Our results demonstrate that SBW can be used as an alternative low-cost substrate for BC production on commercial scale.
以农业副产品大豆乳清(SBW)为原料制备了一种低成本培养基,并用Komagataeibacter sp. PAP1对其进行了优化,用于生产生物纤维素(BC)。产BC的最佳条件为:pH 6.21,乙醇浓度(v/v) 1.61%, 28.4℃。与标准Hestrin-Schramm (HS)培养基相比,使用SBW优化培养基可使BC产量增加3.6倍。与从标准HS介质中产生的膜相比,从优化介质中产生的BC膜更强,更不渗透水蒸气和氧气。这些特性使SBW薄膜发展成为氧气敏感产品的环保食品包装。它的高吸水能力使其在生物医学应用中使用,如伤口敷料。我们的研究结果表明,SBW可以作为一种低成本的衬底用于商业规模的BC生产。
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引用次数: 30
Tiger hair morphology and its variations for wildlife forensic investigation 虎毛形态及其变异在野生动物法医调查中的应用
IF 0.7 4区 综合性期刊 Q2 Multidisciplinary Pub Date : 2013-11-01 DOI: 10.14456/MIJST.2013.36
Thitika Kitpipit, Phuvadol Thanakiatkrai
Tiger population has dramatically decreased due to illegal consumption and commercialisation of their body parts. Frequently, hair samples are the only evidence found in the crime scene. Thus, they play an important role in species identification for wildlife forensic investigation. In this study, we provide the first in-depth report on a variety of qualitative and quantitative characteristics of tiger guard hairs (24 hairs per individual from four individuals). The proposed method could reduce subjectivity of expert opinions on species identification based on hair morphology. Variations in 23 hair morphological characteristics were quantified at three levels: hair section, body region, and intra-species. The results indicate statistically significant variations in most morphological characteristics in all levels. Intra-species variations of four variables, namely hair length, hair index, scale separation and scale pattern, were low. Therefore, identification of tiger hairs using these multiple features in combination with other characteristics with high inter-species variations (e.g. medulla type) should bring about objective and accurate tiger hair identification. The method used should serve as a guideline and be further applied to other species to establish a wildlife hair morphology database. Statistical models could then be constructed to distinguish species and provide evidential values in terms of likelihood ratios.
由于非法消费和老虎身体部位的商业化,老虎的数量急剧减少。通常,头发样本是在犯罪现场发现的唯一证据。因此,它们在野生动物法医调查的物种鉴定中发挥着重要作用。在这项研究中,我们首次深入报道了老虎护毛的各种定性和定量特征(来自4个个体的24根毛发)。该方法可以减少专家意见在基于毛发形态的物种鉴定中的主观性。对23种毛发形态特征的变异进行了定量分析,分为毛发剖面、体区和种内3个层次。结果表明,在所有水平上,大多数形态特征的差异具有统计学意义。毛发长度、毛发指数、鳞片间距和鳞片格局4个变量的种内变异均较低。因此,利用这些多重特征结合其他物种间差异较大的特征(如髓质类型)进行虎毛鉴定,应能实现客观、准确的虎毛鉴定。该方法可作为参考,并进一步应用于其他物种,建立野生动物毛发形态学数据库。然后可以构建统计模型来区分物种,并根据似然比提供证据值。
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引用次数: 3
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Maejo International Journal of Science and Technology
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