Pub Date : 2023-10-17DOI: 10.25081/ripb.2023.v13.8575
Adejoke O. Baderinwa-Adejumo, Taiwo A. Adenegan-Alakinde
The vegetative anatomy of five domesticated species of the genus Cucurbita L. found in South Western Nigeria was studied. The aim was to establish some useful diagnostic features that may be employed in combination with other characters as intra or inter-specific or generic tools for their delimitation. The study revealed exciting features that are helpful in the identification of each species. The genus can be divided into three groups based on the shape of the midrib. Those with a U-shape are C. pepo, C. argyrosperma and C. ficifolia. C. maximum has a round/flat shape while C. moschata has V shape. Those with a U-shape can further be classed into those with a wide and narrow neck. C. argyrosperma has narrow neck with two vascular bundles, four layers of palisade tissues and 6 layers of abaxial epidermis. C. pepo has a wide neck with three bicollateral bundles and two layers of palisade tissues while C. ficifolia has two bundles and three layers of palisade tissues. Overlaps and similarities in the number of vascular bundles, and layers of tissues showed generic affinity among the members.
{"title":"Vegetative anatomy of five domesticated members of the genus Cucurbita (Linn.) in Southwestern Nigeria","authors":"Adejoke O. Baderinwa-Adejumo, Taiwo A. Adenegan-Alakinde","doi":"10.25081/ripb.2023.v13.8575","DOIUrl":"https://doi.org/10.25081/ripb.2023.v13.8575","url":null,"abstract":"The vegetative anatomy of five domesticated species of the genus Cucurbita L. found in South Western Nigeria was studied. The aim was to establish some useful diagnostic features that may be employed in combination with other characters as intra or inter-specific or generic tools for their delimitation. The study revealed exciting features that are helpful in the identification of each species. The genus can be divided into three groups based on the shape of the midrib. Those with a U-shape are C. pepo, C. argyrosperma and C. ficifolia. C. maximum has a round/flat shape while C. moschata has V shape. Those with a U-shape can further be classed into those with a wide and narrow neck. C. argyrosperma has narrow neck with two vascular bundles, four layers of palisade tissues and 6 layers of abaxial epidermis. C. pepo has a wide neck with three bicollateral bundles and two layers of palisade tissues while C. ficifolia has two bundles and three layers of palisade tissues. Overlaps and similarities in the number of vascular bundles, and layers of tissues showed generic affinity among the members.","PeriodicalId":499940,"journal":{"name":"Research in Plant Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-06DOI: 10.25081/ripb.2023.v13.8576
Taiwo Ayomipo Adenegan-Alakinde
This study seeks to use the leaf architecture as a taxonomic tool for members of the genus Basella Linn. in Nigeria, namely, B. rubra Linn., B. alba Linn., B. cordifolia Lamk. and a form, B. alba round because the taxonomy of the genus Basella Linn. is not clear and there is no information on the leaf architecture of the genus. For studies of the venation pattern of the leaves, sizeable portions were taken from the standard median portion of mature leaves. Cleared leaves were stored in 50% alcohol for anatomical studies. Leaves were stained in Safranin O for 3 minutes and counterstained in Alcian blue and then were rinsed in water to remove excess stain. Stained cleared leaves were treated in serial grades of alcohol for differentiation and dehydration. Stained materials were mounted in dilute glycerol for microscopic examination. Photomicrographs were taken with the aid of 3013 ACCU-SCOPE Trinocular Microscope with Digital Camera. The areolar area was calculated from the length and breadth of the areoles measured using a micrometer inserted into the microscope eyepiece. Basella species have common generic features. Areoles are closed in B. rubra but others have veinlet endings. The area of areole is diagnostic because in B. rubra the area is 305,370±1808.91 μm2; B. alba is 501,796.75±3217.38 μm2, B. cordifolia’s area of areole is 396,394.75±2670.67 μm2 while that of B. alba round is 540,058.75±3702.28 μm2. The arrangement of druses in the areoles is also diagnostic.
本研究旨在利用叶结构作为Basella Linn属成员的分类工具。在尼日利亚，即B. rubra LinnB.阿尔巴·林恩。， B. cordifolia Lamk。和一种形式，B. alba round，因为Basella Linn属的分类学。不清楚，也没有关于叶结构属的信息。为了研究叶片的脉纹模式，相当大的部分取自成熟叶片的标准中位数。清除后的叶子保存在50%的酒精中用于解剖研究。叶子用红花红O染色3分钟，用阿利新蓝反染色，然后用水冲洗以去除多余的污渍。染色后的叶片在不同等级的酒精中进行分化和脱水。染色的材料用稀甘油浸泡显微镜检查。显微照片采用3013 ACCU-SCOPE数码相机三眼显微镜拍摄。通过插入显微镜目镜的千分尺测量的光晕的长度和宽度来计算光晕面积。Basella种具有共同的属性特征。红伞的微孔是闭合的，但其他的有静脉末端。乳穴面积为305,370±1808.91 μm2，具有诊断价值;白桦为501,796.75±3217.38 μm2，堇叶白桦圆孔面积为396,394.75±2670.67 μm2，白桦圆孔面积为540,058.75±3702.28 μm2。小孔内药物的排列也可用于诊断。
{"title":"Taxonomic significance of leaf architecture in the genus Basella Linn. in Nigeria","authors":"Taiwo Ayomipo Adenegan-Alakinde","doi":"10.25081/ripb.2023.v13.8576","DOIUrl":"https://doi.org/10.25081/ripb.2023.v13.8576","url":null,"abstract":"This study seeks to use the leaf architecture as a taxonomic tool for members of the genus Basella Linn. in Nigeria, namely, B. rubra Linn., B. alba Linn., B. cordifolia Lamk. and a form, B. alba round because the taxonomy of the genus Basella Linn. is not clear and there is no information on the leaf architecture of the genus. For studies of the venation pattern of the leaves, sizeable portions were taken from the standard median portion of mature leaves. Cleared leaves were stored in 50% alcohol for anatomical studies. Leaves were stained in Safranin O for 3 minutes and counterstained in Alcian blue and then were rinsed in water to remove excess stain. Stained cleared leaves were treated in serial grades of alcohol for differentiation and dehydration. Stained materials were mounted in dilute glycerol for microscopic examination. Photomicrographs were taken with the aid of 3013 ACCU-SCOPE Trinocular Microscope with Digital Camera. The areolar area was calculated from the length and breadth of the areoles measured using a micrometer inserted into the microscope eyepiece. Basella species have common generic features. Areoles are closed in B. rubra but others have veinlet endings. The area of areole is diagnostic because in B. rubra the area is 305,370±1808.91 μm2; B. alba is 501,796.75±3217.38 μm2, B. cordifolia’s area of areole is 396,394.75±2670.67 μm2 while that of B. alba round is 540,058.75±3702.28 μm2. The arrangement of druses in the areoles is also diagnostic.","PeriodicalId":499940,"journal":{"name":"Research in Plant Biology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134943872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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