Pub Date : 2025-01-17DOI: 10.1080/21645698.2024.2448869
Chunlai Wang,Yangyang Zhou,Yimeng Wang,Peng Jiao,Siyan Liu,Shuyang Guan,Yiyong Ma
Maize (Zea mays L.) is a widely grown food crop around the world. Drought stress seriously affects the growth and development process of plants and causes serious damage to maize yield. In the early stage, our research group conducted transcriptome sequencing analysis on the drought-resistant maize inbred line H8186 and screened out a gene with significantly down-regulated expression, Phylloplanin-like (ZmPL1). The ZmPL1 gee expression pattern was analyzed under various abiotic stresses, and the results showed that this gene was greatly affected by drought stress. Subcellular localization analysis showed that the protein was localized on the cell membrane. In order to verify the role of ZmPL1 in drought stress, we overexpressed ZmPL1 in yeast and found that the expression of ZmPL1 could significantly increase the drought sensitivity of yeast. Next, ZmPL1 transgenic plants were obtained by infecting maize callus using Agrobacterium-mediated method. Under drought stress, compared with overexpression lines, gene-edited lines had higher germination rate and seedling survival rate, lower accumulation of MDA, relative conductivity and ROS, higher antioxidant enzyme activity, and the expression levels of stress-related genes and ROS scavenging-related genes were significantly increased. Exogenous application of ABA to each lines under drought stress attenuated the damage caused by drought stress on ZmPL overexpressing plants. In summary, ZmPL1 negatively regulates drought tolerance in maize.
{"title":"CRISPR-Cas9-mediated editing of ZmPL1 gene improves tolerance to drought stress in maize.","authors":"Chunlai Wang,Yangyang Zhou,Yimeng Wang,Peng Jiao,Siyan Liu,Shuyang Guan,Yiyong Ma","doi":"10.1080/21645698.2024.2448869","DOIUrl":"https://doi.org/10.1080/21645698.2024.2448869","url":null,"abstract":"Maize (Zea mays L.) is a widely grown food crop around the world. Drought stress seriously affects the growth and development process of plants and causes serious damage to maize yield. In the early stage, our research group conducted transcriptome sequencing analysis on the drought-resistant maize inbred line H8186 and screened out a gene with significantly down-regulated expression, Phylloplanin-like (ZmPL1). The ZmPL1 gee expression pattern was analyzed under various abiotic stresses, and the results showed that this gene was greatly affected by drought stress. Subcellular localization analysis showed that the protein was localized on the cell membrane. In order to verify the role of ZmPL1 in drought stress, we overexpressed ZmPL1 in yeast and found that the expression of ZmPL1 could significantly increase the drought sensitivity of yeast. Next, ZmPL1 transgenic plants were obtained by infecting maize callus using Agrobacterium-mediated method. Under drought stress, compared with overexpression lines, gene-edited lines had higher germination rate and seedling survival rate, lower accumulation of MDA, relative conductivity and ROS, higher antioxidant enzyme activity, and the expression levels of stress-related genes and ROS scavenging-related genes were significantly increased. Exogenous application of ABA to each lines under drought stress attenuated the damage caused by drought stress on ZmPL overexpressing plants. In summary, ZmPL1 negatively regulates drought tolerance in maize.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"20 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-15DOI: 10.1080/21645698.2025.2450852
Hussein F Hassan,Hasan Yassine,Ahlam Chaaban,Ahmad Chehaitly,Zeinab Skaineh,Nagham Cherri,Sahar Moussawi,Nour Baytamouni,Philippe Hussein Kobeissy,Hani Dimassi,José-Noel Ibrahim
Genetically modified foods (GMFs) have garnered significant attention due to their implications for health, agriculture, and food security. Understanding knowledge and perceptions of GMFs is essential, as these factors influence acceptance and attitudes. This study, the first of its kind in Lebanon, aimed to assess the knowledge, attitudes, and perceptions regarding GMFs and to explore their association with socio-demographic characteristics. A cross-sectional study was conducted with 1,001 participants who completed a 50-item questionnaire, followed by a 15-minute educational session on GMFs. Statistical analysis was performed using SPSS. Prior to the educational session, participants had an average knowledge score of 60.3 ± 17.4%, which significantly increased to 83.0 ± 15.8% afterward. Attitude and perception scores improved from an average of 30.3 ± 25.1% pre-intervention to 38.9 ± 12.4% post-intervention. Females demonstrated significantly higher knowledge scores, and educational attainment was positively correlated with knowledge levels both pre- and post-intervention. Younger participants and those in health-related fields scored higher before the intervention, while unemployed individuals scored lower. Additionally, higher educational levels and health-related educational backgrounds were linked to better attitudes and perceptions pre-intervention, while unemployment correlated with lower scores. Interestingly, multivariate regression analysis indicated that being under 35 years of age, having a health-related educational background, and holding a university degree were predictors of higher GMF knowledge. Consequently, individuals with initially lower knowledge level benefited the most from the educational intervention, exhibiting the greatest knowledge increases post-education. Our findings underscore the importance of targeted educational initiatives to bridge knowledge gaps and address misconceptions regarding GMFs.
{"title":"Public knowledge, attitudes, and perceptions toward genetically modified foods in Lebanon.","authors":"Hussein F Hassan,Hasan Yassine,Ahlam Chaaban,Ahmad Chehaitly,Zeinab Skaineh,Nagham Cherri,Sahar Moussawi,Nour Baytamouni,Philippe Hussein Kobeissy,Hani Dimassi,José-Noel Ibrahim","doi":"10.1080/21645698.2025.2450852","DOIUrl":"https://doi.org/10.1080/21645698.2025.2450852","url":null,"abstract":"Genetically modified foods (GMFs) have garnered significant attention due to their implications for health, agriculture, and food security. Understanding knowledge and perceptions of GMFs is essential, as these factors influence acceptance and attitudes. This study, the first of its kind in Lebanon, aimed to assess the knowledge, attitudes, and perceptions regarding GMFs and to explore their association with socio-demographic characteristics. A cross-sectional study was conducted with 1,001 participants who completed a 50-item questionnaire, followed by a 15-minute educational session on GMFs. Statistical analysis was performed using SPSS. Prior to the educational session, participants had an average knowledge score of 60.3 ± 17.4%, which significantly increased to 83.0 ± 15.8% afterward. Attitude and perception scores improved from an average of 30.3 ± 25.1% pre-intervention to 38.9 ± 12.4% post-intervention. Females demonstrated significantly higher knowledge scores, and educational attainment was positively correlated with knowledge levels both pre- and post-intervention. Younger participants and those in health-related fields scored higher before the intervention, while unemployed individuals scored lower. Additionally, higher educational levels and health-related educational backgrounds were linked to better attitudes and perceptions pre-intervention, while unemployment correlated with lower scores. Interestingly, multivariate regression analysis indicated that being under 35 years of age, having a health-related educational background, and holding a university degree were predictors of higher GMF knowledge. Consequently, individuals with initially lower knowledge level benefited the most from the educational intervention, exhibiting the greatest knowledge increases post-education. Our findings underscore the importance of targeted educational initiatives to bridge knowledge gaps and address misconceptions regarding GMFs.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"24 1","pages":"144-155"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The number of authorized genetically modified (GM) soybeans has increased worldwide. In Japan, 34 GM soybeans containing single events and their stacked varieties have been approved as food. However, not all approved GM events are commercially cultivated or distributed. In this study, we evaluated domestically distributed samples from the United States (US) and Canada using 17 event-specific detection methods for GM soybeans. Identity-preserved (IP) soybean samples imported from the US and Canada, and non-IP samples from the US in 2021 and 2022 were analyzed. Four GM soybean events consisting of MON89788, A5547-127, MON87708, and DAS-44406 were detected in all lots in the non-IP samples. Furthermore, a single-kernel-based analysis was conducted to determine whether the detected GM soybean events are stacked. The results suggest that DAS-44406 is rapidly increasing, particularly as a single event among GM soybeans.
{"title":"Distribution status of genetically modified soybeans from the United States and Canada to Japan in 2021 and 2022.","authors":"Keisuke Soga,Yu Hashimoto,Tomohiro Egi,Chie Taguchi,Satoko Yoshiba,Norihito Shibata,Kazunari Kondo,Reona Takabatake","doi":"10.1080/21645698.2024.2444048","DOIUrl":"https://doi.org/10.1080/21645698.2024.2444048","url":null,"abstract":"The number of authorized genetically modified (GM) soybeans has increased worldwide. In Japan, 34 GM soybeans containing single events and their stacked varieties have been approved as food. However, not all approved GM events are commercially cultivated or distributed. In this study, we evaluated domestically distributed samples from the United States (US) and Canada using 17 event-specific detection methods for GM soybeans. Identity-preserved (IP) soybean samples imported from the US and Canada, and non-IP samples from the US in 2021 and 2022 were analyzed. Four GM soybean events consisting of MON89788, A5547-127, MON87708, and DAS-44406 were detected in all lots in the non-IP samples. Furthermore, a single-kernel-based analysis was conducted to determine whether the detected GM soybean events are stacked. The results suggest that DAS-44406 is rapidly increasing, particularly as a single event among GM soybeans.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"14 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142887913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-23DOI: 10.1080/21645698.2024.2345401
Gideon Sadikiel Mmbando
ABSTRACT Genetically modified (GM) crops are the most important agricultural commodities that can improve the yield of African smallholder farmers. The intricate circumstances surrounding the introduction of GM agriculture in Africa, however, underscore the importance of comprehending the moral conundrums, regulatory environments, and public sentiment that exist today. This review examines the current situation surrounding the use of GM crops in Africa, focusing on moral conundrums, regulatory frameworks, and public opinion. Only eleven of the fifty-four African countries currently cultivate GM crops due to the wide range of opinions resulting from the disparities in cultural, socioeconomic, and environmental factors. This review proposed that addressing public concerns, harmonizing regulations, and upholding ethical standards will improve the adoption of GM crops in Africa. This study offers ways to enhance the acceptability of GM crops for boosting nutrition and food security globally.
{"title":"The Adoption of Genetically Modified Crops in Africa: the Public’s Current Perception, the Regulatory Obstacles, and Ethical Challenges","authors":"Gideon Sadikiel Mmbando","doi":"10.1080/21645698.2024.2345401","DOIUrl":"https://doi.org/10.1080/21645698.2024.2345401","url":null,"abstract":"ABSTRACT Genetically modified (GM) crops are the most important agricultural commodities that can improve the yield of African smallholder farmers. The intricate circumstances surrounding the introduction of GM agriculture in Africa, however, underscore the importance of comprehending the moral conundrums, regulatory environments, and public sentiment that exist today. This review examines the current situation surrounding the use of GM crops in Africa, focusing on moral conundrums, regulatory frameworks, and public opinion. Only eleven of the fifty-four African countries currently cultivate GM crops due to the wide range of opinions resulting from the disparities in cultural, socioeconomic, and environmental factors. This review proposed that addressing public concerns, harmonizing regulations, and upholding ethical standards will improve the adoption of GM crops in Africa. This study offers ways to enhance the acceptability of GM crops for boosting nutrition and food security globally.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"60 6","pages":"1 - 15"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140668055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-16DOI: 10.1080/21645698.2024.2339568
Shengdong Yang, Gang Wang, Minghui Niu, Heng Zhang, Jing Ma, Chunpu Qu, Guanjun Liu
Poplar stands as one of the primary afforestation trees globally. We successfully generated transgenic poplar trees characterized by enhanced biomass under identical nutrient conditions, through th...
{"title":"Impacts of AlaAT3 transgenic poplar on rhizosphere soil chemical properties, enzyme activity, bacterial community, and metabolites under two nitrogen conditions","authors":"Shengdong Yang, Gang Wang, Minghui Niu, Heng Zhang, Jing Ma, Chunpu Qu, Guanjun Liu","doi":"10.1080/21645698.2024.2339568","DOIUrl":"https://doi.org/10.1080/21645698.2024.2339568","url":null,"abstract":"Poplar stands as one of the primary afforestation trees globally. We successfully generated transgenic poplar trees characterized by enhanced biomass under identical nutrient conditions, through th...","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140556661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-08DOI: 10.1080/21645698.2024.2335701
Stuart J. Smyth, Peter W. B. Phillips, David Castle
This article provides an analysis and evaluation of peer-reviewed evidence on the contribution of crop biotechnology to climate change mitigation and adaption. While there is a range of agricultura...
{"title":"An assessment of the linkages between GM crop biotechnology and climate change mitigation","authors":"Stuart J. Smyth, Peter W. B. Phillips, David Castle","doi":"10.1080/21645698.2024.2335701","DOIUrl":"https://doi.org/10.1080/21645698.2024.2335701","url":null,"abstract":"This article provides an analysis and evaluation of peer-reviewed evidence on the contribution of crop biotechnology to climate change mitigation and adaption. While there is a range of agricultura...","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140538360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soybean is one of the important oil crops and a major source of protein and lipids. Drought can cause severe soybean yields. Dehydrin protein (DHN) is a subfamily of LEA proteins that play an impor...
{"title":"Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic Arabidopsis","authors":"Jiayi Fan, Yuzhe Zhang, Hongji Sun, Ruijie Duan, Yushi Jiang, Xinyu Wang, Yao Sun, Zhipeng Luo, Peiwu Wang, Shuyan Guan, Siyan Liu, Xuhong Fan, Peng Jiao, Yunpeng Wang, Jinhui Yang, Zunyue Zhang, Huiwei Yu","doi":"10.1080/21645698.2024.2327116","DOIUrl":"https://doi.org/10.1080/21645698.2024.2327116","url":null,"abstract":"Soybean is one of the important oil crops and a major source of protein and lipids. Drought can cause severe soybean yields. Dehydrin protein (DHN) is a subfamily of LEA proteins that play an impor...","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140346257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.1080/21645698.2024.2325180
William Foster, Jorge Ortega, Gonzalo Vargas
We estimate producer and consumer surplus changes due to a possible GM maize import ban in Chile, which produces only non-GM grains for internal use. Without foreign non-GM sources, the ban’s effec...
{"title":"Modeling the economic impact for Chile of an import ban on genetically modified maize","authors":"William Foster, Jorge Ortega, Gonzalo Vargas","doi":"10.1080/21645698.2024.2325180","DOIUrl":"https://doi.org/10.1080/21645698.2024.2325180","url":null,"abstract":"We estimate producer and consumer surplus changes due to a possible GM maize import ban in Chile, which produces only non-GM grains for internal use. Without foreign non-GM sources, the ban’s effec...","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"141 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140164664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.1080/21645698.2023.2290752
Inez Z. Ponce de Leon
GM technology has constantly faced opposition in the Philippines. The debate heightened in 2016, when the Supreme Court halted the field testing of Bt eggplant, which triggered a public debate. Thi...
{"title":"A struggle for control beyond the facts: examining constructs of GM technology in Philippine opinion columns","authors":"Inez Z. Ponce de Leon","doi":"10.1080/21645698.2023.2290752","DOIUrl":"https://doi.org/10.1080/21645698.2023.2290752","url":null,"abstract":"GM technology has constantly faced opposition in the Philippines. The debate heightened in 2016, when the Supreme Court halted the field testing of Bt eggplant, which triggered a public debate. Thi...","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138771013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-15DOI: 10.1080/21645698.2023.2275723
Madeline M. Esquivel, Siddique I. Aboobucker, Walter P. Suza
Genetically modified (GM) crops offer significant advantages in our crop improvement programs because they are created using a more targeted approach which is not possible in traditional breeding m...
{"title":"The impact of ‘framing’ in the adoption of GM crops","authors":"Madeline M. Esquivel, Siddique I. Aboobucker, Walter P. Suza","doi":"10.1080/21645698.2023.2275723","DOIUrl":"https://doi.org/10.1080/21645698.2023.2275723","url":null,"abstract":"Genetically modified (GM) crops offer significant advantages in our crop improvement programs because they are created using a more targeted approach which is not possible in traditional breeding m...","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138657569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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