首页 > 最新文献

Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology最新文献

英文 中文
Identification of rosmarinic acid as a novel antidepressive substance in the leaves of Perilla frutescens Britton var. acuta Kudo (Perillae Herba). 从 Perilla frutescens Britton var. acuta Kudo(Perillae Herba)叶片中鉴定出新型抗抑郁物质迷迭香酸。
Hiroshi Takeda, Minoru Tsuji, Teruhiko Matsumiya, Masayoshi Kubo

The leaves of Perilla frutescens Britton var. acuta Kudo (Perillae Herba) are found in some traditional oriental herbal medicines that are primarily used to treat affective disorders such as depression and anxiety. The aim of the present study was to identify the bioactive component in Perillae Herba that possesses antidepressive activity. The effects of a water extract of Perillae Herba and six fractions therefrom were evaluated in mice by use of the forced-swimming test. An oral administration of a water extract of Perillae Herba significantly reduced the duration of immobility. Moreover, 50% methanol extract of the water extract of Perillae Herba and its 30% methanol extract also reduced the duration of immobility. Three-dimensional high-performance liquid chromatography and FAB-MS and NMR spectral analysis clearly showed that the extracts with anti-immobility effects contained abundant rosmarinic acid. The oral and intraperitoneal administration of rosmarinic acid significantly reduced the duration of immobility. In contrast, a water extract from another species of Perillae Herba, which contains only low levels of rosmarinic acid, had no anti-immobility effect. These results suggest that rosmarinic acid may be the main component involved in the antidepressive effect of Perillae Herba in the forced-swimming test. Thus it may be a novel antidepressive substance.

紫苏叶(Perilla frutescens Britton var.本研究旨在确定紫苏中具有抗抑郁活性的生物活性成分。研究人员通过强迫小鼠游泳试验,评估了紫苏水提取物及其六种馏分对小鼠的影响。口服紫苏水萃取物后,小鼠不动的时间明显缩短。此外,从紫苏水提取物中提取的 50%甲醇提取物及其 30% 甲醇提取物也能缩短静止不动的时间。三维高效液相色谱法、FAB-MS 和 NMR 光谱分析清楚地表明,具有抗静电作用的提取物中含有丰富的迷迭香酸。口服和腹腔注射迷迭香酸能显著缩短不运动的持续时间。与此相反,另一种紫苏属植物的水提取物只含有少量的迷迭香酸,却没有抗静电作用。这些结果表明,迷迭香酸可能是紫苏在强迫游泳试验中发挥抗抑郁作用的主要成分。因此,它可能是一种新型抗抑郁物质。
{"title":"Identification of rosmarinic acid as a novel antidepressive substance in the leaves of Perilla frutescens Britton var. acuta Kudo (Perillae Herba).","authors":"Hiroshi Takeda, Minoru Tsuji, Teruhiko Matsumiya, Masayoshi Kubo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The leaves of Perilla frutescens Britton var. acuta Kudo (Perillae Herba) are found in some traditional oriental herbal medicines that are primarily used to treat affective disorders such as depression and anxiety. The aim of the present study was to identify the bioactive component in Perillae Herba that possesses antidepressive activity. The effects of a water extract of Perillae Herba and six fractions therefrom were evaluated in mice by use of the forced-swimming test. An oral administration of a water extract of Perillae Herba significantly reduced the duration of immobility. Moreover, 50% methanol extract of the water extract of Perillae Herba and its 30% methanol extract also reduced the duration of immobility. Three-dimensional high-performance liquid chromatography and FAB-MS and NMR spectral analysis clearly showed that the extracts with anti-immobility effects contained abundant rosmarinic acid. The oral and intraperitoneal administration of rosmarinic acid significantly reduced the duration of immobility. In contrast, a water extract from another species of Perillae Herba, which contains only low levels of rosmarinic acid, had no anti-immobility effect. These results suggest that rosmarinic acid may be the main component involved in the antidepressive effect of Perillae Herba in the forced-swimming test. Thus it may be a novel antidepressive substance.</p>","PeriodicalId":502118,"journal":{"name":"Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology","volume":"22 1","pages":"15-22"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[BMAL1 and circadian rhythm]. [BMAL1与昼夜节律]
M Ikeda

hBmal1 cDNA has been cloned from a human brain cDNA library and revealed that the cDNA encoded a novel bHLH-PAS transcription factor. The rBmal1 mRNA expression was found not only at high levels in the suprachiasmatic nuclei (SCN), but also in the pyriform cortex, hippocampus, and cerebellum. Furthermore, this expression was found in the eyes, pineal body, and peripheral organs, such as the muscle, liver, and heart. Bmal1 mRNA levels reveal a circadian rhythm in the rat SCN, with the highest expression levels at ZT18. The yeast two-hybrid screening have demonstrated that CLOCK forms a heterodimer with BMAL1. CLOCK. BMAL1 heterodimer functions as a positive transcription factor through binding to the E-box in the promoter region of the mPer1. Nuclear translocation is promoted by dimerization among PERs, or between PERs and CRYs, and the complexes inhibit the transcription of their own genes. This feedback loop is used in the clock output system driving the transcription of arginine vasopressin peptide and serotonin N-acetyl-transferase by binding the CLOCK-BMAL1 dimer to an E-box in their promoters.

从人脑 cDNA 文库中克隆出了 hBmal1 cDNA,并发现该 cDNA 编码一种新型 bHLH-PAS 转录因子。rBmal1 mRNA不仅在嗜上核(SCN)中有高水平表达,而且在梨状皮层、海马和小脑中也有高水平表达。此外,在眼睛、松果体以及肌肉、肝脏和心脏等外周器官中也发现了这种表达。Bmal1 mRNA水平显示了大鼠SCN的昼夜节律,在ZT18表达水平最高。酵母双杂交筛选证明,CLOCK与BMAL1形成异源二聚体。CLOCK。BMAL1异源二聚体通过与mPer1启动子区的E-box结合,发挥正转录因子的功能。PERs 之间或 PERs 与 CRYs 之间的二聚体可促进核转位,复合物可抑制自身基因的转录。这种反馈回路用于时钟输出系统,通过将 CLOCK-BMAL1 二聚体与启动子中的 E-box 结合,驱动精氨酸加压素肽和血清素 N-乙酰转移酶的转录。
{"title":"[BMAL1 and circadian rhythm].","authors":"M Ikeda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>hBmal1 cDNA has been cloned from a human brain cDNA library and revealed that the cDNA encoded a novel bHLH-PAS transcription factor. The rBmal1 mRNA expression was found not only at high levels in the suprachiasmatic nuclei (SCN), but also in the pyriform cortex, hippocampus, and cerebellum. Furthermore, this expression was found in the eyes, pineal body, and peripheral organs, such as the muscle, liver, and heart. Bmal1 mRNA levels reveal a circadian rhythm in the rat SCN, with the highest expression levels at ZT18. The yeast two-hybrid screening have demonstrated that CLOCK forms a heterodimer with BMAL1. CLOCK. BMAL1 heterodimer functions as a positive transcription factor through binding to the E-box in the promoter region of the mPer1. Nuclear translocation is promoted by dimerization among PERs, or between PERs and CRYs, and the complexes inhibit the transcription of their own genes. This feedback loop is used in the clock output system driving the transcription of arginine vasopressin peptide and serotonin N-acetyl-transferase by binding the CLOCK-BMAL1 dimer to an E-box in their promoters.</p>","PeriodicalId":502118,"journal":{"name":"Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology","volume":"20 5","pages":"203-12"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Molecular mechanism of biological clock: for chronobiological approach to stress]. [生物钟的分子机制:针对压力的时间生物学方法]。
T Ebisawa

Recent progress in molecular chronobiology revealed that the clock genes control intracellular feedback loops. CLOCK protein and BMAL1 protein, first discovered as components of the circadian clock in mammals, are known to function as transcriptional activators in the circadian feedback loop of drosophila. PERIOD and TIMELESS proteins work as inhibitors for these activators in drosophila and possibly in mammals. The clock genes described above are also expressed in peripheral tissues with circadian rhythmicity. Cultured rat-1 fibroblast shows circadian expression of clock genes after serum shock or forskolin stimulation. These results indicate that the clock genes function not only as components of the endogenous clock, but also as a coordinator of the circadian activity of peripheral tissues. It will be important to study stress from the aspect of circadian rhythm.

分子时间生物学的最新进展表明,时钟基因控制着细胞内的反馈回路。CLOCK 蛋白和 BMAL1 蛋白是最早发现的哺乳动物昼夜节律钟的组成成分,它们在果蝇的昼夜节律反馈环路中发挥转录激活剂的作用。在果蝇和哺乳动物中,PERIOD 蛋白和 TIMELESS 蛋白是这些激活因子的抑制剂。上述时钟基因在具有昼夜节律性的外周组织中也有表达。培养的大鼠-1 成纤维细胞在受到血清休克或福斯克林刺激后显示出时钟基因的昼夜节律表达。这些结果表明,时钟基因不仅是内源性时钟的组成部分,而且还是外周组织昼夜节律活动的协调者。从昼夜节律的角度研究压力将是非常重要的。
{"title":"[Molecular mechanism of biological clock: for chronobiological approach to stress].","authors":"T Ebisawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recent progress in molecular chronobiology revealed that the clock genes control intracellular feedback loops. CLOCK protein and BMAL1 protein, first discovered as components of the circadian clock in mammals, are known to function as transcriptional activators in the circadian feedback loop of drosophila. PERIOD and TIMELESS proteins work as inhibitors for these activators in drosophila and possibly in mammals. The clock genes described above are also expressed in peripheral tissues with circadian rhythmicity. Cultured rat-1 fibroblast shows circadian expression of clock genes after serum shock or forskolin stimulation. These results indicate that the clock genes function not only as components of the endogenous clock, but also as a coordinator of the circadian activity of peripheral tissues. It will be important to study stress from the aspect of circadian rhythm.</p>","PeriodicalId":502118,"journal":{"name":"Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology","volume":"20 3","pages":"107-11"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1