Pub Date : 2023-12-18DOI: 10.18502/jehsd.v8i4.14438
Fatemeh Atoof, Sahar Gholipour, Zahra Shamsizadeh, Mohsen Amirimoghaddam, N. Mirzaei, Ali Nazari-Alam, D. Rabbani, M. Baziar, G. Hoseindoost, Gholamreza Mostafaii, Abbas Bahrami
Introduction: Given that hospital air is one of the important environmental sources for transmission of microorganisms, the importance of airborne transmission in the epidemiology of hospital-acquired infections (HAIs) has gained attention in the past two decades. Therefore, the present study aims to determine the concentration of bacteria in association with airborne particulate matter (PM) in the outdoor and indoor air of two hospital wards. Materials and methods: The GRIMM 1.109 dust monitor and the Andersen one-stage viable impactor were used for particle counting and bioaerosol sampling, respectively. Results: The average levels of airborne bacteria sampled from outdoor air were 33 colony-forming units (CFU/m3), and in the air samples of medical and infectious disease wards, they were 76 and 85 CFU/m3, respectively. Staphylococcus spp. and Acinetobacter spp. were the most prevalent bacteria in the samples. Statistical analysis showed a significant association between PM2.5, and PM10 particle mass concentrations and airborne bacteria concentrations in indoor air samples (P-value < 0.05). Conclusion: Some bacterial agents of HAIs existed in hospital air and may be problematic for immunocompromised patients. Higher levels of bacteria in indoor air compared to outdoor air may indicate that the bacteria were of indoor origin, such as the presence and activities of people. Moreover, the results showed that particle counting may be a useful tool for airborne bacteria monitoring.
{"title":"Characteristics of Airborne Particles and Bacteria in Hospital Indoor and Outdoor Air","authors":"Fatemeh Atoof, Sahar Gholipour, Zahra Shamsizadeh, Mohsen Amirimoghaddam, N. Mirzaei, Ali Nazari-Alam, D. Rabbani, M. Baziar, G. Hoseindoost, Gholamreza Mostafaii, Abbas Bahrami","doi":"10.18502/jehsd.v8i4.14438","DOIUrl":"https://doi.org/10.18502/jehsd.v8i4.14438","url":null,"abstract":"Introduction: Given that hospital air is one of the important environmental sources for transmission of microorganisms, the importance of airborne transmission in the epidemiology of hospital-acquired infections (HAIs) has gained attention in the past two decades. Therefore, the present study aims to determine the concentration of bacteria in association with airborne particulate matter (PM) in the outdoor and indoor air of two hospital wards. Materials and methods: The GRIMM 1.109 dust monitor and the Andersen one-stage viable impactor were used for particle counting and bioaerosol sampling, respectively. Results: The average levels of airborne bacteria sampled from outdoor air were 33 colony-forming units (CFU/m3), and in the air samples of medical and infectious disease wards, they were 76 and 85 CFU/m3, respectively. Staphylococcus spp. and Acinetobacter spp. were the most prevalent bacteria in the samples. Statistical analysis showed a significant association between PM2.5, and PM10 particle mass concentrations and airborne bacteria concentrations in indoor air samples (P-value < 0.05). Conclusion: Some bacterial agents of HAIs existed in hospital air and may be problematic for immunocompromised patients. Higher levels of bacteria in indoor air compared to outdoor air may indicate that the bacteria were of indoor origin, such as the presence and activities of people. Moreover, the results showed that particle counting may be a useful tool for airborne bacteria monitoring.","PeriodicalId":502531,"journal":{"name":"Journal of Environmental Health and Sustainable Development","volume":"31 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139172956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-18DOI: 10.18502/jehsd.v8i4.14437
Shahnaz Sargazi, A. Ebrahimi, Seyed Mehdi Tabatabaei, M. Ehrampoush, Ramin Saravani, M. Z. Sakhvidi, Hossein Fallahzadeh, Sheida Shahraki
Introduction: The two main causes of indoor air pollution (IAP) are cooking and smoking. Toxic substances, including polycyclic aromatic hydrocarbons (PAHs), recognized as human carcinogens, are present in cooking and tobacco smoke. This study aims to determine the cytotoxicity and genotoxicity of PAHs collected from the indoor air of coffee shops on human cell line (KB/C152) in vitro approach. Materials and methods: Sampling pumps at a 2 l/min flow rate for 5 hours were applied to collect indoor air samples. Next, KB cells in cell culture medium were exposed to different concentrations of extracted PAHs using Methyl Thiazolyl Tetrazolium (MTT) test. Finally, terminal dUTP nick-end labeling (TUNEL) test and cell cycle assessments were both examined using flow cytometry. Results: The MTT test revealed a significant cytotoxic effect on KB cells depending on the PAHs concentrationas compared to the control cell line. The lethal concentration 50 (LC50) value against KB cells was 100 ± 3.09 µg/ml. Accordingly, exposure to extracted PAHs resulted in an arrest in the cell cycle at the sub-G1 checkpoint. The extracted PAHs suppressed the cell cycle in the sub-G1 phase, damaged DNA, and arrested KB cells from proliferating. Additionally, a statistically significant increase in DNA cleavage percentages (p ≤ 0.05) was seen in the TUNEL test, which also showed a dose-dependent increase in DNA damage. Conclusion: Extracted PAHs caused DNA damage and arrested in the cell cycle in epidermoid carcinoma of the mouth cells (KB/C152) in vitro mechanisms. This evaluation highlights mechanisms of exposure to extracted PAHs and their detrimental health effects.
{"title":"Effect of Polycyclic Aromatic Hydrocarbons Extracted from Indoor Air of Coffee Shops on Oral Squamous Carcinoma Cell (KB/C152): An in Vitro Approach","authors":"Shahnaz Sargazi, A. Ebrahimi, Seyed Mehdi Tabatabaei, M. Ehrampoush, Ramin Saravani, M. Z. Sakhvidi, Hossein Fallahzadeh, Sheida Shahraki","doi":"10.18502/jehsd.v8i4.14437","DOIUrl":"https://doi.org/10.18502/jehsd.v8i4.14437","url":null,"abstract":"Introduction: The two main causes of indoor air pollution (IAP) are cooking and smoking. Toxic substances, including polycyclic aromatic hydrocarbons (PAHs), recognized as human carcinogens, are present in cooking and tobacco smoke. This study aims to determine the cytotoxicity and genotoxicity of PAHs collected from the indoor air of coffee shops on human cell line (KB/C152) in vitro approach. Materials and methods: Sampling pumps at a 2 l/min flow rate for 5 hours were applied to collect indoor air samples. Next, KB cells in cell culture medium were exposed to different concentrations of extracted PAHs using Methyl Thiazolyl Tetrazolium (MTT) test. Finally, terminal dUTP nick-end labeling (TUNEL) test and cell cycle assessments were both examined using flow cytometry. Results: The MTT test revealed a significant cytotoxic effect on KB cells depending on the PAHs concentrationas compared to the control cell line. The lethal concentration 50 (LC50) value against KB cells was 100 ± 3.09 µg/ml. Accordingly, exposure to extracted PAHs resulted in an arrest in the cell cycle at the sub-G1 checkpoint. The extracted PAHs suppressed the cell cycle in the sub-G1 phase, damaged DNA, and arrested KB cells from proliferating. Additionally, a statistically significant increase in DNA cleavage percentages (p ≤ 0.05) was seen in the TUNEL test, which also showed a dose-dependent increase in DNA damage. Conclusion: Extracted PAHs caused DNA damage and arrested in the cell cycle in epidermoid carcinoma of the mouth cells (KB/C152) in vitro mechanisms. This evaluation highlights mechanisms of exposure to extracted PAHs and their detrimental health effects.","PeriodicalId":502531,"journal":{"name":"Journal of Environmental Health and Sustainable Development","volume":"43 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-18DOI: 10.18502/jehsd.v8i4.14436
Kosar Daneshipour, Armin Naghipour, A. Rad
Introduction: Since global warming and air pollution was caused by human activities have increased dramatically in recent decades, studies have been conducted to determine how environmental parameters and air pollutants interact and, subsequently, how these pollutants affect ecosystems and human health. Accordingly, this study aimed to investigate the relationship between temperature and air pollutants in Tehran, Iran, in order to determine whether warming is associated with an increase in air pollutants. Materials and Methods: During 2015-2019, Iran Air Quality Monitoring Station (AQMS) and the Landsat 8 satellite were employed to retrieve data on pollutants such as PM, CO, O3, NO2, SO2, and air quality index (AQI), as well as climate-related metrics including ambient temperature and land surface temperature (LST). Pearson correlation coefficient and linear regression model were used to analyze the data. Results: A positive correlation was found between temperature variables and PM10 (ρ = 0.29, p = 0.001) and O3 (ρ= 0.55, p = 0.001) pollutants. PM10 and O3 levels were evaluated by 0.46 (95% Confidence Interval (CI) = (0.25, 0.67), p = 0.001) and 1.13 (95% CI = 0.89, 1.37), p = 0.001) units for each one-unit rise in temperature. Conclusion: Given the fact that temperature predominantly evidenced a significant synergistic association with PM10 and O3, it was determined that there was no substantial positive association between all pollutants and warming.
{"title":"The Relationships between Air Pollution, Warming, and Health in Tehran Metropolis, Iran, during 2015–2019","authors":"Kosar Daneshipour, Armin Naghipour, A. Rad","doi":"10.18502/jehsd.v8i4.14436","DOIUrl":"https://doi.org/10.18502/jehsd.v8i4.14436","url":null,"abstract":"Introduction: Since global warming and air pollution was caused by human activities have increased dramatically in recent decades, studies have been conducted to determine how environmental parameters and air pollutants interact and, subsequently, how these pollutants affect ecosystems and human health. Accordingly, this study aimed to investigate the relationship between temperature and air pollutants in Tehran, Iran, in order to determine whether warming is associated with an increase in air pollutants. Materials and Methods: During 2015-2019, Iran Air Quality Monitoring Station (AQMS) and the Landsat 8 satellite were employed to retrieve data on pollutants such as PM, CO, O3, NO2, SO2, and air quality index (AQI), as well as climate-related metrics including ambient temperature and land surface temperature (LST). Pearson correlation coefficient and linear regression model were used to analyze the data. Results: A positive correlation was found between temperature variables and PM10 (ρ = 0.29, p = 0.001) and O3 (ρ= 0.55, p = 0.001) pollutants. PM10 and O3 levels were evaluated by 0.46 (95% Confidence Interval (CI) = (0.25, 0.67), p = 0.001) and 1.13 (95% CI = 0.89, 1.37), p = 0.001) units for each one-unit rise in temperature. Conclusion: Given the fact that temperature predominantly evidenced a significant synergistic association with PM10 and O3, it was determined that there was no substantial positive association between all pollutants and warming.","PeriodicalId":502531,"journal":{"name":"Journal of Environmental Health and Sustainable Development","volume":"30 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139173040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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