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Relationships among cortisol, perceived stress, and dental caries experience in adolescents and young adults. 青少年皮质醇、感知压力和龋齿经历之间的关系。
Pub Date : 2024-04-24 DOI: 10.1159/000539041
Naima Abouseta, Noha Gomaa, Ali Tassi, Abdelbaset A. Elzagallaai, Michael J. Rieder, S. J. Dixon, S. Pani
INTRODUCTIONStress can impact mental and physical health, especially during adolescence and young adulthood. Previous studies have shown that salivary cortisol levels are elevated in both severe dental caries and periodontal disease. The role of stress in periodontal disease has been studied, but the extent of its contribution to dental caries is less well understood, especially in this age group. The present study aimed to assess the association between perceived stress, cortisol levels (in hair and saliva) and overall caries experience of adolescents and young adults aged between 15 and 25 years.METHODSHair and saliva samples were obtained from 93 participants (age range=15-25 years) free of periodontal disease. Cortisol concentrations in hair and saliva were determined using a competitive enzyme-linked immunosorbent assay. Participants completed a perceived stress questionnaire and underwent full-mouth oral examination by a calibrated examiner. Dental caries experience was based on the decayed, missing, and filled teeth (DMFT) index. In addition, sociodemographic variables were recorded. Univariate and multivariable binary logistic regressions were used to assess the relationships of interest.RESULTSThere were significantly higher hair cortisol levels (p=0.042) and perceived stress scale (PSS) scores (p=0.026) in individuals with dental caries experience (DMFT≥1) than in those without (DMFT=0). However, there was no significant difference in salivary cortisol concentration (p=0.302). A binary logistic regression revealed that higher hair cortisol levels and greater scores on the perceived stress scale were associated with an increased odds of having experienced dental caries (OR=4.08, 95% CI 1.04, 15.96; OR=1.65, 95% CI 1.04, 2.63; respectively). In contrast, no significant association was found between dental caries and salivary cortisol concentration (OR=0.31, 95% CI 0.02, 4.23). Using multivariable regression models, caries experience was found to be significantly associated with both hair cortisol levels and PSS scores. These associations remained statistically significant even after adjusting for confounding sociodemographic variables.CONCLUSIONIn the absence of periodontal disease, hair cortisol levels have a significant association with dental caries experience, whereas salivary cortisol concentrations do not. Hair cortisol levels may reflect the chronic physiological burden imposed by exposure to detrimental stressors.
引言 压力会影响身心健康,尤其是在青春期和青年期。以往的研究表明,严重龋齿和牙周病患者的唾液皮质醇水平都会升高。压力在牙周病中的作用已被研究过,但其对龋齿的影响程度却不甚了解,尤其是在这个年龄段。本研究旨在评估感知到的压力、皮质醇水平(头发和唾液中)与 15-25 岁青少年总体龋齿经历之间的关联。采用竞争性酶联免疫吸附试验测定头发和唾液中的皮质醇浓度。参与者填写了一份感知压力问卷,并接受了由校准检查员进行的全口口腔检查。龋齿情况以蛀牙、缺失牙和补牙(DMFT)指数为依据。此外,还记录了社会人口学变量。结果有龋齿经历者(DMFT≥1)的毛发皮质醇水平(p=0.042)和感知压力量表(PSS)得分(p=0.026)明显高于无龋齿经历者(DMFT=0)。然而,唾液皮质醇浓度没有明显差异(P=0.302)。二元逻辑回归结果显示,毛发皮质醇水平越高、感知压力量表得分越高,发生龋齿的几率就越大(OR=4.08,95% CI 1.04,15.96;OR=1.65,95% CI 1.04,2.63;分别如此)。相比之下,龋齿与唾液皮质醇浓度之间并无明显关联(OR=0.31,95% CI 0.02,4.23)。使用多变量回归模型发现,龋齿经历与毛发皮质醇水平和 PSS 评分均有显著关联。结论 在没有牙周病的情况下,毛发皮质醇水平与龋齿经历有明显的关联,而唾液皮质醇浓度则没有。毛发皮质醇水平可能反映了暴露于有害压力源所造成的慢性生理负担。
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引用次数: 0
Evaluation of Streptococcus mutans biofilm formation and acidogenicity of infant milk formulas for treating cow milk allergy: An in vitro study. 评估用于治疗牛奶过敏的婴儿配方奶粉的变异链球菌生物膜形成和致酸性:体外研究。
Pub Date : 2024-04-18 DOI: 10.1159/000538882
Navaphan Rattanapakdeekul, J. Lapirattanakul, Orada Tosrisawatkasem, R. Surarit, A. Smutkeeree
INTRODUCTIONWhen infants cannot consume breast milk, the most commonly available alternative milk formula is cow milk-based. Due to a rise in the prevalence of cow milk protein allergy (CMPA) among children, this study aimed to assess the biofilm formation and acidogenicity of cow milk-based formulas as well as milk formulas suggested for children with CMPA.METHODSCow milk-based formulas with 0%, 10%, or 18% sucrose added, partially hydrolyzed formula (pHF), extensively hydrolyzed formula (eHF), amino acid-based formula (AAF), and soy-based formulas with 0%, or 11% sucrose added were evaluated. Streptococcus mutans was used as a representative microorganism associated with caries. The acidogenicity after 24-h incubation was assessed by the pH of the formed biofilm and lactic acid formation. Biofilm formation was quantified using crystal violet staining. Additionally, the biofilm characteristics were determined using confocal laser scanning microscopy (CLSM). Comparisons were made among formulas without added sucrose to observe protein-based differences. Furthermore, formulas with different sucrose percentages were compared to explore the impact of sucrose content.RESULTSWhen comparing the formulas without added sucrose, the biofilm formation in the cow milk-based formula and pHF were significantly greater than the soy-based formula, eHF, and AAF. In the presence of S. mutans, all formulas reduced the biofilm pH below the critical enamel pH. The cow milk-based formula and AAF showed a significantly lower biofilm pH than the pHF, soy-based, and eHF groups, while the lactic acid production was markedly higher in the cow milk-based formula, pHF and AAF, compared with the eHF and soy-based formula. Adding sucrose into the cow milk-based and soy-based formulas substantially increased biofilm mass. The biofilm pH of the cow milk-based formulas, with or without sucrose, was significantly lower than that of the soy-based formulas. The CLSM indicated distinct biofilm characteristics among the different protein-based formulas, with sucrose supplementation promoting S. mutans aggregation in cow milk-based formula biofilm and increased density and intact biofilm in the soy-based formula.CONCLUSIONAll assessed milk formulas had caries-inducing factors, including those without supplemental sucrose. Among them, the eHF demonstrated the least caries-inducing factors, attributed to its minimal biofilm formation and the highest biofilm pH.
导言当婴儿不能食用母乳时,最常见的替代配方奶粉是以牛奶为基础的配方奶粉。方法对添加 0%、10% 或 18% 蔗糖的牛奶配方奶粉、部分水解配方奶粉 (pHF)、广泛水解配方奶粉 (eHF)、氨基酸配方奶粉 (AAF) 以及添加 0% 或 11% 蔗糖的大豆配方奶粉进行了评估。变异链球菌被用作与龋齿有关的代表性微生物。培养 24 小时后,通过所形成的生物膜的 pH 值和乳酸的形成来评估酸性。使用水晶紫染色法对生物膜的形成进行量化。此外,还使用共焦激光扫描显微镜(CLSM)测定了生物膜的特征。对未添加蔗糖的配方进行了比较,以观察基于蛋白质的差异。结果在比较未添加蔗糖的配方奶粉时,以牛奶为基础的配方奶粉和 pHF 的生物膜形成明显多于以大豆为基础的配方奶粉、eHF 和 AAF。在存在变异沙门氏菌的情况下,所有配方奶粉都能将生物膜的 pH 值降至临界珐琅质 pH 值以下。牛奶配方和 AAF 的生物膜 pH 值明显低于 pHF、大豆配方和 eHF 组,而与 eHF 和大豆配方相比,牛奶配方、pHF 和 AAF 的乳酸产生量明显更高。在牛奶配方和大豆配方中添加蔗糖会大大增加生物膜的质量。无论是否添加蔗糖,牛奶配方奶粉的生物膜 pH 值都明显低于大豆配方奶粉。CLSM 显示,不同的蛋白质配方奶粉具有不同的生物膜特征,补充蔗糖会促进牛奶配方奶粉生物膜中的变异单胞菌聚集,而大豆配方奶粉中的生物膜密度和完整度会增加。其中,eHF 的诱发龋齿因子最少,这是因为它的生物膜形成最少,生物膜 pH 值最高。
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引用次数: 0
Association between Sense of Coherence and Caries Activity and Caries Experience among Male Adolescents from Southern Brazil: A Cross-Sectional Study. 巴西南部男性青少年的一致性感与龋齿活动和龋齿经历之间的关系:一项横断面研究。
Pub Date : 2024-04-17 DOI: 10.1159/000538488
N. Lock, M. L. Gazola, P. K. Marquezan, J. E. Zenkner, L. Alves
INTRODUCTIONThis cross-sectional study assessed the association between sense of coherence (SoC) and caries activity (number of active caries lesions) and caries experience (DMFT index) among 18-19-year-old male adolescents who joined the Brazilian Army as draftees for mandatory military service (n = 507).METHODSData collection included a questionnaire (level of education, family income, and tooth brushing frequency), the Brazilian short version of the SoC scale (SOC-13), and clinical caries examination (noncavitated/cavitated, inactive/active). The main predictor variable was SoC, categorized as low, moderate, or high. Poisson regression models were used for statistical analysis.RESULTSA high SoC was significantly associated with a lower number of active lesions (adjusted rate ratio = 0.85; 95% CI = 0.74-0.98). No association between SoC and DMFT was detected.CONCLUSIONA high SoC was found to be a protective factor to caries activity in this population.
简介:本横断面研究评估了作为义务兵加入巴西军队的 18-19 岁男性青少年(507 人)的一致性感(SoC)与龋病活动(活动性龋损数量)和龋病经历(DMFT 指数)之间的关系。方法数据收集包括问卷调查(教育水平、家庭收入和刷牙频率)、巴西短版 SoC 量表(SOC-13)和临床龋齿检查(非龋性/龋性、非活动性/活动性)。SoC是主要的预测变量,分为低、中、高三个等级。结果高SoC与较低的活动性病变数量显著相关(调整后比率比=0.85;95% CI=0.74-0.98)。结论在该人群中,高SoC是龋病活动的保护因素。
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引用次数: 0
Should We Wait to Brush Our Teeth?: A Scoping Review Regarding Dental Caries and Erosive Tooth Wear. 我们是否应该等待刷牙?关于龋齿和腐蚀性牙齿磨损的范围研究。
Pub Date : 2024-04-15 DOI: 10.1159/000538862
Constanza Fernández, Consuelo A Silva-Acevedo, Fernanda Padilla-Orellana, D. Zero, T. S. Carvalho, Adrian Lussi
BACKGROUNDTooth brushing is a universal recommendation. However, the recommendations related to the time of its execution are conflicting, especially when dealing with patients at risk of erosive tooth wear (ETW) or dental caries.SUMMARYOur objective was to summarize the evidence on the timing of brushing with fluoridated toothpaste in relation to ETW and cariogenic dietary challenges. We conducted a scoping review following the PRISMA-ScR checklist, using three databases searching for in-vivo, in-situ, or in-vitro studies involving human teeth exposed to either a cariogenic or an erosive challenge. Only models including human saliva and fluoride were assessed. Data selection, extraction, and risk of bias analysis were done in duplicate and independently. From 1,545 identified studies, 17 (16 related to ETW and 1 to dental caries) were included. Most evidence (n=10) supported that brushing with a fluoride-containing product does not increase ETW, independent of the moment of brushing. Delaying tooth brushing up to 1 hour (n=4) or individualized recommendations based on the patient's problem (n=2) were less frequent. Only one study reported that brushing pre- or post-meal does not affect Streptococcus mutans counts. Most data was in-situ (n=13), and the overall study quality was judged as sufficient/low risk of bias.KEY MESSAGESAlthough the available evidence lacked robust clinical studies, tooth brushing using fluoridated products immediately after an erosive challenge does not increase the risk of ETW and can be recommended, which is in line with recommendations for dental caries prevention. Furthermore, we suggest updating the international guidelines to promote individualized recommendations based on risk factors to prevent either ETW or dental caries.
背景刷牙是一项普遍建议。摘要我们的目的是总结有关使用含氟牙膏刷牙的时间与 ETW 和致龋饮食挑战相关的证据。我们按照 PRISMA-ScR 清单进行了范围界定综述,利用三个数据库搜索了涉及暴露于致癌或腐蚀性挑战的人类牙齿的体内、原位或体外研究。仅对包含人类唾液和氟化物的模型进行了评估。数据的选择、提取和偏倚风险分析均独立重复完成。在已确定的 1545 项研究中,有 17 项(16 项与 ETW 有关,1 项与龋齿有关)被纳入。大多数证据(n=10)支持使用含氟产品刷牙不会增加ETW,与刷牙时间无关。推迟刷牙时间长达 1 小时(4 项)或根据患者的问题提出个性化建议(2 项)的研究较少。只有一项研究报告称,餐前或餐后刷牙不会影响变异链球菌的数量。虽然现有证据缺乏可靠的临床研究,但在受到侵蚀性挑战后立即使用含氟产品刷牙不会增加发生 ETW 的风险,因此可以推荐使用含氟产品,这与预防龋齿的建议是一致的。此外,我们建议更新国际指南,提倡根据风险因素提供个性化建议,以预防ETW或龋齿。
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引用次数: 0
Risk of bias and methodological critical appraisal in systematic reviews of non- and micro-invasive caries management for primary and permanent teeth. 对原牙和恒牙的非侵入性和微侵入性龋病管理进行系统性回顾时的偏差风险和方法学批判性评估。
Pub Date : 2024-04-04 DOI: 10.1159/000537749
Caroline Mariano Laux, R. Elagami, Adriana Dos Santos, Ana Paula Pires Dos Santos, T. Tedesco, Thais Gimenez, D. Raggio
INTRODUCTIONPaediatric dentistry should rely on evidence-based clinical decisions supported by high-quality, unbiased systematic reviews (SRs). Therefore, the purpose of this study was to systematically evaluate the methodological quality and risk of bias of SRs focused on non- and micro-invasive treatment for caries lesions in primary and permanent teeth.METHODSA comprehensive search was conducted in multiple databases, including MEDLINE/PubMed, Scopus, Web of Science, EMBASE, Epistemonikos, and ProQuest, up to March 2023 to identify relevant systematic reviews (SRs) focused on non- and micro-invasive caries treatment. Two independent reviewers extracted data from the included SRs and assessed the methodological quality and risk of bias using the AMSTAR 2 and ROBIS tools, respectively.RESULTSA total of 39 SRs were included in the analysis. Among these, 27 SRs (69.2%) were assessed as having critically low methodological quality, 11 SRs (28.2%) were considered to have low methodological quality and only one SR was rated as high-quality. The primary concern identified was the absence of protocol registration before the commencing the study, observed in 33 SR when using the AMSTAR 2 tool. According to the ROBIS tool, 21 studies (53.8%) were categorised as low risk of bias, 10 (25.6%) as high risk and eight (20.5%) as unclear risk of bias.CONCLUSIONOur analysis revealed that SRs focused on non- and micro-invasive treatment for caries in children and adolescents had critically low methodological quality according to the AMSTAR 2 tool but demonstrated a low risk of bias based on the ROBIS tool. These findings highlight the importance of emphasizing prospective protocol registration, clear reporting of statistical analyses, and addressing potential bias implications within this topic. By addressing these issues, we can enhance the quality of SRs and ensure that clinical decisions rely on unbiased and trustworthy evidence. Registry DOI: 10.17605/OSF.IO/AR4MS.
引言儿童牙科应依靠高质量、无偏见的系统综述(SR)支持的循证临床决策。方法截至 2023 年 3 月,我们在多个数据库(包括 MEDLINE/PubMed、Scopus、Web of Science、EMBASE、Epistemonikos 和 ProQuest)中进行了全面检索,以确定以非侵入性和微侵入性龋病治疗为重点的相关系统综述 (SR)。两位独立审稿人从纳入的SR中提取了数据,并分别使用AMSTAR 2和ROBIS工具评估了方法学质量和偏倚风险。其中,27 份样本报告(69.2%)被评为方法学质量极低,11 份样本报告(28.2%)被认为方法学质量较低,只有一份样本报告被评为高质量。在使用 AMSTAR 2 工具时发现,33 份研究报告在研究开始前没有进行方案注册,这是发现的主要问题。根据 ROBIS 工具,21 项研究(53.8%)被归类为低偏倚风险,10 项研究(25.6%)被归类为高风险,8 项研究(20.5%)被归类为不明确的偏倚风险。结论我们的分析表明,根据 AMSTAR 2 工具,侧重于儿童和青少年龋病的非侵入性和微侵入性治疗的 SR 的方法学质量极低,但根据 ROBIS 工具,其偏倚风险较低。这些发现凸显了强调前瞻性方案注册、清晰报告统计分析以及解决本专题中潜在的偏倚影响的重要性。通过解决这些问题,我们可以提高SR的质量,确保临床决策依赖于无偏见、可信赖的证据。注册表 DOI:10.17605/OSF.IO/AR4MS.
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引用次数: 0
A right to health-based approach to dental caries: towards a comprehensive control strategy. 以健康权为基础的龋齿防治方法:制定综合防治战略。
Pub Date : 2024-04-02 DOI: 10.1159/000538459
Sarienten Dominique Mollet, David John Manton, Jan Wollgast, B. Toebes
BACKGROUNDHealth is a matter of human rights and dental caries is the most common non-communicable disease (NCD) globally. Consequently, dental caries is a matter of human rights and its control, particularly prevention, must be a priority. Although largely preventable, this is too often neglected, both in the literature of human rights- and health law, and in dental research. The right to oral health has recently been acknowledged by the World Health Organization (WHO), but it is insufficiently clear what this right entails.SUMMARYThis article introduces a right to health-based narrative in the context of dental caries. The right to health is stipulated in human rights treaties, including the International Covenant on Economic, Social and Cultural Rights (ICESCR) and the Convention on the Rights of the Child (CRC). States that are Parties to these treaties, which are virtually all States globally, are mandated to ensure the enjoyment of individuals' right to the highest attainable standard of health, including oral health.KEY MESSAGESDental caries is a matter of human rights. States have binding obligations to address dental caries: they require the regulation of the healthcare system, i.e. the traditional focus on operative care, but also put the regulation of other risk factors on an equal footing, such as the regulation of the living environment and access to fluoride. A right to health-based approach to dental caries thus offers a comprehensive approach to dental caries control, particularly prevention.
背景健康是人权问题,而龋齿是全球最常见的非传染性疾病(NCD)。因此,龋齿是一个人权问题,其控制,尤其是预防,必须成为优先事项。虽然龋齿在很大程度上是可以预防的,但无论是在人权和卫生法文献中,还是在牙科研究中,龋齿都常常被忽视。世界卫生组织(WHO)最近承认了口腔健康权,但这一权利的内涵还不够明确。包括《经济、社会、文化权利国际公约》(ICESCR)和《儿童权利公约》(CRC)在内的人权条约都规定了健康权。这些条约的缔约国(几乎是全球所有国家)有义务确保个人享有能达到的最高标准健康的权利,包括口腔健康。各国有义务解决龋齿问题:它们要求对医疗保健系统进行监管,即传统上以手术治疗为重点,但也对其他风险因素进行同等监管,如生活环境监管和氟化物的获取。因此,以健康权为基础的龋齿防治方法提供了一种全面的龋齿防治方法,特别是预防方法。
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Caries Research
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