Pub Date : 2024-05-08DOI: 10.47352/jmans.2774-3047.210
Awalul Fatiqin, R. Alfanaar, Sudarman Rahman, Yahya Febrianto, Shesanti Citrariana, Mu’afa Purwa Arsana, T. Suprayogi, Y. S. Kurniawan
Drymoglossum piloselloides is one of the epiphytic plants that is commonly found in Southeast Asia region. In this study, the ethanol extract of D. piloselloides plant has been used in the green synthesis of silver nanoparticles. The synthesized silver nanoparticles were characterized by ultraviolet-visible (UV-Vis) spectrophotometry, X-ray diffraction (XRD),Fourier-transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM) measurements. The UV-Vis spectrum of silver nanoparticles showed a maximum wavelength at 453 nm. The XRD measurement showed the silver nanoparticles peaks at 38.38°, 44.60°, 64.76°, and 77.62°. The FTIR spectra provided evidence of the interaction between silver and chemicals in the plant extract as a weak signal at 682 cm-1. Meanwhile, TEM revealed an average size of 12.63nm. The synthesised silver nanoparticles were utilised for the reduction of 4-nitrophenol with a conversion percentage of up to 100% with a reduction reaction rate constant of 7.104 s-1. In addition, methylene blue was also successfully reduced with the synthesised silver nanoparticles as the catalyst with a reduction reaction rate constant (k) of 21.150 s-1. This study highlights the superior advantage of utilizing ethanolic extract of D. piloselloides to prepare silver nanoparticles with promising catalytic reduction purposes.
{"title":"Catalytic Reduction of 4-Nitrophenol and Methylene Blue with Silver Nanoparticles Decorated with Drymoglossum piloselloides Extract","authors":"Awalul Fatiqin, R. Alfanaar, Sudarman Rahman, Yahya Febrianto, Shesanti Citrariana, Mu’afa Purwa Arsana, T. Suprayogi, Y. S. Kurniawan","doi":"10.47352/jmans.2774-3047.210","DOIUrl":"https://doi.org/10.47352/jmans.2774-3047.210","url":null,"abstract":"Drymoglossum piloselloides is one of the epiphytic plants that is commonly found in Southeast Asia region. In this study, the ethanol extract of D. piloselloides plant has been used in the green synthesis of silver nanoparticles. The synthesized silver nanoparticles were characterized by ultraviolet-visible (UV-Vis) spectrophotometry, X-ray diffraction (XRD),Fourier-transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM) measurements. The UV-Vis spectrum of silver nanoparticles showed a maximum wavelength at 453 nm. The XRD measurement showed the silver nanoparticles peaks at 38.38°, 44.60°, 64.76°, and 77.62°. The FTIR spectra provided evidence of the interaction between silver and chemicals in the plant extract as a weak signal at 682 cm-1. Meanwhile, TEM revealed an average size of 12.63nm. The synthesised silver nanoparticles were utilised for the reduction of 4-nitrophenol with a conversion percentage of up to 100% with a reduction reaction rate constant of 7.104 s-1. In addition, methylene blue was also successfully reduced with the synthesised silver nanoparticles as the catalyst with a reduction reaction rate constant (k) of 21.150 s-1. This study highlights the superior advantage of utilizing ethanolic extract of D. piloselloides to prepare silver nanoparticles with promising catalytic reduction purposes.","PeriodicalId":506457,"journal":{"name":"Journal of Multidisciplinary Applied Natural Science","volume":" 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140999669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-31DOI: 10.47352/jmans.2774-3047.205
Rodhiansyah Djayasinga, R. Situmeang, F. Unob, S. Hadi, Posman Manurung, S. Sumardi
Identifying the most effective material with antibacterial properties against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) is a challenging task considering the rising concerns about drug resistance. Various experiments through in vitro and in vivo studies to obtain antibacterial agents using abundant and easily available raw material sources have been conducted. Therefore, this study aimed to acquire semiconducting nanoparticle material derived from purebred chicken eggshell waste that could effectively function as an antibacterial agent. The waste treatment was carried out using a top-down method applying the thermal decomposition method with calcination temperatures of 700 and 800 ºC for 30 hours. XRD analysis results showed CaO as a major phase and this was further supported by Rietveld calculation. The size of the crystalline phases obtained ranged from 10–45 nm, while FTIR analysis showed the appearance of CaO bond at a wave number of 715.65 cm-1. Furthermore, SEM analysis showed a rough folded particle surface with a pore percentage of 48.20%. Based on the UV-Vis DRS analysis results, chicken eggshell powder had band gap energy characteristics of 2.07, 2.74, 3.71, and 5.96 eV for sample B, as well as 4.60 and 5.82 eV for sample C. Activation of purebred chicken eggshell powder as antibacterial was performed both qualitatively and quantitatively using photocatalytic and non-photocatalytic methods. Qualitatively, both samples showed antibacterial activity, with a minimal inhibitory concentration (MIC) of 1,000 µg/mL.
考虑到人们对耐药性的日益关注，确定对金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）最有效的抗菌材料是一项具有挑战性的任务。为了利用丰富且易于获得的原料来源获得抗菌剂，已经通过体外和体内研究进行了各种实验。因此，本研究旨在从纯种鸡蛋壳废弃物中获取可有效用作抗菌剂的半导体纳米粒子材料。废物处理采用自上而下的热分解法，煅烧温度分别为 700 ºC 和 800 ºC，煅烧时间为 30 小时。XRD 分析结果表明 CaO 是主要相，Rietveld 计算进一步证实了这一点。所得结晶相的尺寸范围为 10-45 nm，而傅立叶变换红外分析则显示在波数为 715.65 cm-1 时出现了 CaO 键。此外，扫描电镜分析表明，颗粒表面粗糙折叠，孔隙率为 48.20%。根据紫外可见 DRS 分析结果，样品 B 的带隙能特性分别为 2.07、2.74、3.71 和 5.96 eV，样品 C 的带隙能特性分别为 4.60 和 5.82 eV。在定性方面，两种样品都显示出抗菌活性，最小抑菌浓度（MIC）均为 1,000 µg/mL。
{"title":"Chicken Eggshell Powder as Antibacterial Against Staphylococcus aureus and Escherichia coli Through In Vitro Studies","authors":"Rodhiansyah Djayasinga, R. Situmeang, F. Unob, S. Hadi, Posman Manurung, S. Sumardi","doi":"10.47352/jmans.2774-3047.205","DOIUrl":"https://doi.org/10.47352/jmans.2774-3047.205","url":null,"abstract":"Identifying the most effective material with antibacterial properties against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) is a challenging task considering the rising concerns about drug resistance. Various experiments through in vitro and in vivo studies to obtain antibacterial agents using abundant and easily available raw material sources have been conducted. Therefore, this study aimed to acquire semiconducting nanoparticle material derived from purebred chicken eggshell waste that could effectively function as an antibacterial agent. The waste treatment was carried out using a top-down method applying the thermal decomposition method with calcination temperatures of 700 and 800 ºC for 30 hours. XRD analysis results showed CaO as a major phase and this was further supported by Rietveld calculation. The size of the crystalline phases obtained ranged from 10–45 nm, while FTIR analysis showed the appearance of CaO bond at a wave number of 715.65 cm-1. Furthermore, SEM analysis showed a rough folded particle surface with a pore percentage of 48.20%. Based on the UV-Vis DRS analysis results, chicken eggshell powder had band gap energy characteristics of 2.07, 2.74, 3.71, and 5.96 eV for sample B, as well as 4.60 and 5.82 eV for sample C. Activation of purebred chicken eggshell powder as antibacterial was performed both qualitatively and quantitatively using photocatalytic and non-photocatalytic methods. Qualitatively, both samples showed antibacterial activity, with a minimal inhibitory concentration (MIC) of 1,000 µg/mL.","PeriodicalId":506457,"journal":{"name":"Journal of Multidisciplinary Applied Natural Science","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140475335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.47352/jmans.2774-3047.204
Christina Astutiningsih, Tias Eka Rahmawati, Nanik Astutik Rahman, Meri Meri
Nanoparticles are extensively studied for their promising biological properties. In this study, the fruit extract of Abelmoschus esculenthus L. was used as a chelating agent for the synthesis of zinc oxide nanoparticles (ZnOPs-AE) using a zinc acetate solution. The prepared ZnOPs-AE were identified and characterized using UV-vis spectroscopy, Fourier-transformed infrared spectroscopy (FTIR), particle size analyzer (PSA), scanning electron microscopy (SEM), and energy dispersive spectrum (EDS). The green synthesized ZnOPs-AE were evaluated for their antioxidant, photoprotective, anti-inflammatory, and antibacterial activities. The synthesized nanoparticles showed an intensity peak at 370 nm in the UV-vis spectrum. The FTIR result shows the presence of O-H, C=O, C-O, C-OH, and C=C chelating functional groups on the surface of nanoparticles. The size of ZnOPs-AE was determined using a PSA with particle size distribution of 102.2 nm. The ZnOPs-AE were shown to be spherical by SEM analysis and composition was 82.11% and 14.79% for Zn and O, respectively. The antioxidant properties of ZnOPs-AE showed significant antioxidant potential in DPPH, ABTS, and FRAP assays compared to the quercetin standard. The photoprotection activity test showed a SPF value of 19.63, the percentage of erythema transmission was 5.98%, and the percentage of pigmentation transmission was 5.62%. The ZnOPs-AE showed good anti-inflammatory with the synthesized nanoparticle performing activity between positive control and the fruit extract of Abelmoschus esculenthus L. Also, the ZnOPs-AE exhibited good antibacterial activity against Staphylococcus aureus (20.78 mm) and Pseudomonas aeruginosae (11.13 mm). Overall, the results highlight the effectiveness and potential of ZnOPs-AE for biological application.
{"title":"Green Synthesis of ZnO Nanoparticles using Abelmoschus esculenthus L. Fruit Extract: Antioxidant, Photoprotective, Anti-inflammatory, and Antibacterial Studies","authors":"Christina Astutiningsih, Tias Eka Rahmawati, Nanik Astutik Rahman, Meri Meri","doi":"10.47352/jmans.2774-3047.204","DOIUrl":"https://doi.org/10.47352/jmans.2774-3047.204","url":null,"abstract":"Nanoparticles are extensively studied for their promising biological properties. In this study, the fruit extract of Abelmoschus esculenthus L. was used as a chelating agent for the synthesis of zinc oxide nanoparticles (ZnOPs-AE) using a zinc acetate solution. The prepared ZnOPs-AE were identified and characterized using UV-vis spectroscopy, Fourier-transformed infrared spectroscopy (FTIR), particle size analyzer (PSA), scanning electron microscopy (SEM), and energy dispersive spectrum (EDS). The green synthesized ZnOPs-AE were evaluated for their antioxidant, photoprotective, anti-inflammatory, and antibacterial activities. The synthesized nanoparticles showed an intensity peak at 370 nm in the UV-vis spectrum. The FTIR result shows the presence of O-H, C=O, C-O, C-OH, and C=C chelating functional groups on the surface of nanoparticles. The size of ZnOPs-AE was determined using a PSA with particle size distribution of 102.2 nm. The ZnOPs-AE were shown to be spherical by SEM analysis and composition was 82.11% and 14.79% for Zn and O, respectively. The antioxidant properties of ZnOPs-AE showed significant antioxidant potential in DPPH, ABTS, and FRAP assays compared to the quercetin standard. The photoprotection activity test showed a SPF value of 19.63, the percentage of erythema transmission was 5.98%, and the percentage of pigmentation transmission was 5.62%. The ZnOPs-AE showed good anti-inflammatory with the synthesized nanoparticle performing activity between positive control and the fruit extract of Abelmoschus esculenthus L. Also, the ZnOPs-AE exhibited good antibacterial activity against Staphylococcus aureus (20.78 mm) and Pseudomonas aeruginosae (11.13 mm). Overall, the results highlight the effectiveness and potential of ZnOPs-AE for biological application.","PeriodicalId":506457,"journal":{"name":"Journal of Multidisciplinary Applied Natural Science","volume":"170 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140483586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-22DOI: 10.47352/jmans.2774-3047.198
T. Budhy, Deddy Adam, Zulikran Moh Rizki Azis, Vania Syahputri, M. G. A. Yuliani, Muhammad Febriano Sugiarso Suwarto, Fery Setiawan
Oral cancer is currently the sixth leading malignancy in the world, with over 330,000 cases resulting in death. Several cytokines and proteins protect the survival of cancer cells, such as TNF-α, HSP27, and IL-10. Moringa oleifera is an herbal medicine with anti-cancer properties. Nanoparticles of M. oleifera have the property to be easily absorbed by water-soluble cells, so only small doses are needed to be used as anti-cancer ingredients. This research aims to prove the ability of M. oleifera nanoparticle extract against oral cancer through the expression of tumour necrosis factor α (TNF-α), heat shock protein (HSP27), and interleukin 10 (IL-10). Rat as non-human experimental subjects were divided into four groups: control group (K), treatment group 1 (P1), treatment group 2 (P2), and treatment group 3 (P3). Cancer induction was carried out by injecting with benzo[a]pyrene, and then M. oleifera nanoparticle extract was administered in three forms of treatment doses of 125 μg/mL (P1), 250 μg/mL (P2), and 500 μg/mL (P3). Immunohistochemical examination was analysed through TNF-α, HSP27, and IL-10’s expression. The expression of TNF-α and HSP27 between control and treatment groups was significantly different. P2 had the lowest expression of TNF-α and HSP27. The expression of IL-10 between control and treatment groups was also significantly different. P1 had the lowest expression of IL-10. M. oleifera nanoparticle extract can reduce oral cancer progression by decreasing the expression of TNF-α, HSP27, and IL-10.
{"title":"Potential of Moringa Leaf Nanoparticles (Moringa oleifera) on the Expression of TNFα, IL10, and HSP 27 in Oral Cavity Cancer","authors":"T. Budhy, Deddy Adam, Zulikran Moh Rizki Azis, Vania Syahputri, M. G. A. Yuliani, Muhammad Febriano Sugiarso Suwarto, Fery Setiawan","doi":"10.47352/jmans.2774-3047.198","DOIUrl":"https://doi.org/10.47352/jmans.2774-3047.198","url":null,"abstract":"Oral cancer is currently the sixth leading malignancy in the world, with over 330,000 cases resulting in death. Several cytokines and proteins protect the survival of cancer cells, such as TNF-α, HSP27, and IL-10. Moringa oleifera is an herbal medicine with anti-cancer properties. Nanoparticles of M. oleifera have the property to be easily absorbed by water-soluble cells, so only small doses are needed to be used as anti-cancer ingredients. This research aims to prove the ability of M. oleifera nanoparticle extract against oral cancer through the expression of tumour necrosis factor α (TNF-α), heat shock protein (HSP27), and interleukin 10 (IL-10). Rat as non-human experimental subjects were divided into four groups: control group (K), treatment group 1 (P1), treatment group 2 (P2), and treatment group 3 (P3). Cancer induction was carried out by injecting with benzo[a]pyrene, and then M. oleifera nanoparticle extract was administered in three forms of treatment doses of 125 μg/mL (P1), 250 μg/mL (P2), and 500 μg/mL (P3). Immunohistochemical examination was analysed through TNF-α, HSP27, and IL-10’s expression. The expression of TNF-α and HSP27 between control and treatment groups was significantly different. P2 had the lowest expression of TNF-α and HSP27. The expression of IL-10 between control and treatment groups was also significantly different. P1 had the lowest expression of IL-10. M. oleifera nanoparticle extract can reduce oral cancer progression by decreasing the expression of TNF-α, HSP27, and IL-10.","PeriodicalId":506457,"journal":{"name":"Journal of Multidisciplinary Applied Natural Science","volume":"522 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139247880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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