Pub Date : 2025-01-01Epub Date: 2025-02-06DOI: 10.1038/s44294-024-00052-w
Camran R Nezhat, Tomiko T Oskotsky, Joshua F Robinson, Susan J Fisher, Angie Tsuei, Binya Liu, Juan C Irwin, Brice Gaudilliere, Marina Sirota, David K Stevenson, Linda C Giudice
Endometriosis is an enigmatic disease whose diagnosis and management are being transformed through innovative surgical, molecular, and computational technologies. Integrating single-cell and other omic disease data with clinical and surgical metadata can identify multiple disease subtypes with translation to novel diagnostics and therapeutics. Herein, we present real-world perspectives on endometriosis and the importance of multidisciplinary collaboration in informing molecular, epidemiologic, and cell-specific data in the clinical and surgical contexts.
{"title":"Real world perspectives on endometriosis disease phenotyping through surgery, omics, health data, and artificial intelligence.","authors":"Camran R Nezhat, Tomiko T Oskotsky, Joshua F Robinson, Susan J Fisher, Angie Tsuei, Binya Liu, Juan C Irwin, Brice Gaudilliere, Marina Sirota, David K Stevenson, Linda C Giudice","doi":"10.1038/s44294-024-00052-w","DOIUrl":"10.1038/s44294-024-00052-w","url":null,"abstract":"<p><p>Endometriosis is an enigmatic disease whose diagnosis and management are being transformed through innovative surgical, molecular, and computational technologies. Integrating single-cell and other omic disease data with clinical and surgical metadata can identify multiple disease subtypes with translation to novel diagnostics and therapeutics. Herein, we present real-world perspectives on endometriosis and the importance of multidisciplinary collaboration in informing molecular, epidemiologic, and cell-specific data in the clinical and surgical contexts.</p>","PeriodicalId":520241,"journal":{"name":"npj women's health","volume":"3 1","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11802455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01Epub Date: 2025-01-03DOI: 10.1038/s44294-024-00049-5
Zhexian Sun, Wenjie Wu, Zezhen Xiang, Hansong Gao, Weina Ju, Cherilyn Uhm, Ian S Hagemann, Pamela K Woodard, Nanbert Zhong, Alison G Cahill, Qing Wang, Yong Wang
Besides exchanging nutrients, gases, and wastes, placenta is an intermediary between maternal and fetal immune systems. However, no method exists to safely image and monitor placental inflammation during pregnancy. We customized a Magnetic Resonance Imaging (MRI) method, diffusion basis spectrum imaging (DBSI), to measure immune cells in placenta. We validated placental DBSI in simulations and ex-vivo specimens, then applied it to 202 MRI scans from 82 patients whose placentas were classified as non-inflammation (n = 70) or inflammation (n = 12). Our method imaged the 3D distribution of immune cells, revealing significantly greater infiltration in the inflammation placentas from early (2.8% ± 0.7% vs. 4.8% ± 0.65%, p < 0.01) to late pregnancy (4.75% ± 0.9% vs. 7.25% ± 2.13%, p < 0.01). DBSI detects elevated immune cell infiltration associated with placental inflammation and enables non-invasive imaging of placental inflammation, offering early detection and monitoring throughout pregnancy, facilitating personalized care and potentially improving pregnancy outcomes without ionizing radiation.
除了交换营养物质、气体和废物外，胎盘还是母体和胎儿免疫系统之间的中介。然而，没有方法存在安全地成像和监测妊娠期间胎盘炎症。我们定制了一种磁共振成像（MRI）方法，扩散基谱成像（DBSI），来测量胎盘中的免疫细胞。我们在模拟和离体标本中验证了胎盘DBSI，然后将其应用于82例胎盘被分类为非炎症（n = 70）或炎症（n = 12）的患者的202次MRI扫描。我们的方法对免疫细胞的三维分布进行成像，显示早期炎症胎盘的浸润明显增加(2.8%±0.7% vs. 4.8%±0.65%,p p
{"title":"Quantitative and longitudinal assessment of human placental inflammation using diffusion basis spectrum imaging.","authors":"Zhexian Sun, Wenjie Wu, Zezhen Xiang, Hansong Gao, Weina Ju, Cherilyn Uhm, Ian S Hagemann, Pamela K Woodard, Nanbert Zhong, Alison G Cahill, Qing Wang, Yong Wang","doi":"10.1038/s44294-024-00049-5","DOIUrl":"https://doi.org/10.1038/s44294-024-00049-5","url":null,"abstract":"<p><p>Besides exchanging nutrients, gases, and wastes, placenta is an intermediary between maternal and fetal immune systems. However, no method exists to safely image and monitor placental inflammation during pregnancy. We customized a Magnetic Resonance Imaging (MRI) method, diffusion basis spectrum imaging (DBSI), to measure immune cells in placenta. We validated placental DBSI in simulations and ex-vivo specimens, then applied it to 202 MRI scans from 82 patients whose placentas were classified as non-inflammation (<i>n</i> = 70) or inflammation (<i>n</i> = 12). Our method imaged the 3D distribution of immune cells, revealing significantly greater infiltration in the inflammation placentas from early (2.8% ± 0.7% vs. 4.8% ± 0.65%, <i>p</i> < 0.01) to late pregnancy (4.75% ± 0.9% vs. 7.25% ± 2.13%, <i>p</i> < 0.01). DBSI detects elevated immune cell infiltration associated with placental inflammation and enables non-invasive imaging of placental inflammation, offering early detection and monitoring throughout pregnancy, facilitating personalized care and potentially improving pregnancy outcomes without ionizing radiation.</p>","PeriodicalId":520241,"journal":{"name":"npj women's health","volume":"3 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11698687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01Epub Date: 2025-01-29DOI: 10.1038/s44294-025-00054-2
Ola Gutzeit, Aakanksha Gulati, Zohreh Izadifar, Anna Stejskalova, Hassan Rhbiny, Justin Cotton, Bogdan Budnik, Sanjid Shahriar, Girija Goyal, Abidemi Junaid, Donald E Ingber
This study explores the protective role of cervicovaginal mucus in maintaining vaginal health, particularly in relation to bacterial vaginosis (BV), using organ chip technology. By integrating human Cervix and Vagina Chips, we demonstrated that cervical mucus significantly reduces inflammation and epithelial damage caused by a dysbiotic microbiome commonly associated with BV. Proteomic analysis of the Vagina Chip, following exposure to mucus from the Cervix Chip, revealed differentially abundant proteins, suggesting potential biomarkers and therapeutic targets for BV management. Our findings highlight the essential function of cervical mucus in preserving vaginal health and underscore the value of organ chip models for studying complex interactions within the female reproductive tract. This research provides new insights into the mechanisms underlying vaginal dysbiosis and opens avenues for developing targeted therapies and diagnostic tools to enhance women's reproductive health.
{"title":"Cervical mucus in linked human Cervix and Vagina Chips modulates vaginal dysbiosis.","authors":"Ola Gutzeit, Aakanksha Gulati, Zohreh Izadifar, Anna Stejskalova, Hassan Rhbiny, Justin Cotton, Bogdan Budnik, Sanjid Shahriar, Girija Goyal, Abidemi Junaid, Donald E Ingber","doi":"10.1038/s44294-025-00054-2","DOIUrl":"10.1038/s44294-025-00054-2","url":null,"abstract":"<p><p>This study explores the protective role of cervicovaginal mucus in maintaining vaginal health, particularly in relation to bacterial vaginosis (BV), using organ chip technology. By integrating human Cervix and Vagina Chips, we demonstrated that cervical mucus significantly reduces inflammation and epithelial damage caused by a dysbiotic microbiome commonly associated with BV. Proteomic analysis of the Vagina Chip, following exposure to mucus from the Cervix Chip, revealed differentially abundant proteins, suggesting potential biomarkers and therapeutic targets for BV management. Our findings highlight the essential function of cervical mucus in preserving vaginal health and underscore the value of organ chip models for studying complex interactions within the female reproductive tract. This research provides new insights into the mechanisms underlying vaginal dysbiosis and opens avenues for developing targeted therapies and diagnostic tools to enhance women's reproductive health.</p>","PeriodicalId":520241,"journal":{"name":"npj women's health","volume":"3 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11779628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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