Ziyan Wang, Kristin M Lindgren, Jennifer J Loros, Jay C Dunlap
Some cell biological studies of Neurospora crassa have been limited by the rapid rates of hyphal growth and fusion. In this study, we investigated the causative mutation in the standard C24 allele of for (FGSC #9) and assayed the growth and circadian phenotype of the for strain under different nutritional conditions. We show that the for strain can be maintained as metabolically active single cells for 2 days before its growth advances into branched mycelia. This culturing system offers the potential to advance subcellular dynamic research and to facilitate greater understanding of N. crassa in the early developmental stages.
一些对冠突孢属（Neurospora crassa）的细胞生物学研究因其快速的头状花序生长和融合速度而受到限制。在本研究中，我们研究了for（FGSC #9）的标准C24等位基因的致病突变，并检测了for菌株在不同营养条件下的生长和昼夜表型。我们发现，for菌株可以作为代谢活跃的单细胞维持2天，然后再长成分枝菌丝体。这种培养系统为推进亚细胞动态研究提供了潜力，并有助于更好地了解 N. crassa 的早期发育阶段。
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