PeerJ organic chemistry最新文献

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For Catching-by-Polymerization Oligo Purification: Scalable Synthesis of the Precursors to the Polymerizable Tagging Phosphoramidites. 低聚捕集纯化：可聚合标记磷酰胺前驱体的规模化合成。

PeerJ organic chemistry

Pub Date : 2024-01-01 Epub Date: 2024-10-01 DOI: 10.7717/peerj-ochem.12

Yipeng Yin, Komal Chillar, Alexander Apostle, Bhaskar Halami, Adikari M D N Eriyagama, Marina Tanasova, Shiyue Fang

The catching-by-polymerization (CBP) oligodeoxynucleotide (oligo or ODN) purification method has been demonstrated suitable for large-scale, parallel, and long oligo purification. The authenticity of the oligos has been verified via DNA sequencing, and gene construction and expression. A remaining obstacle to the practical utility of the CBP method is affordable polymerizable tagging phosphoramidites (PTPs) that are needed for the method. In this article, we report scalable synthesis of the four nucleoside (dA, dC, dG and T) precursors to the PTPs using a route having five steps from inexpensive starting materials. The overall yields ranged from 21% to 35%. The scales of the synthesis presented here are up to 2.1 grams of the precursors. Because the syntheses are chromatography-free, further scaling up the syntheses of the precursors have become more feasible. With the precursors, the PTPs can be synthesized in one step using standard methods involving a chromatography purification.

聚合捕集（CBP）寡脱氧核苷酸（oligo或ODN）纯化方法已被证明适用于大规模、平行和长寡核苷酸纯化。通过DNA测序、基因构建和表达验证了寡核苷酸的真实性。CBP方法实际应用的另一个障碍是该方法需要可负担得起的可聚合标记磷酸酯（PTPs）。在这篇文章中，我们报道了四种核苷（dA, dC， dG和T）前体到PTPs的可扩展合成，使用五步的路线，从廉价的起始材料。总体收益率在21%至35%之间。这里展示的合成尺度高达2.1克前体。由于合成是不需要色谱的，进一步扩大前体合成的规模变得更加可行。有了前体，PTPs可以用包括色谱纯化的标准方法一步合成。

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