Pub Date : 2021-08-18DOI: 10.1186/s44149-021-00014-8
B. Ayele, S. Leta
{"title":"Knowledge and preventive practices of livestock farmers on infectious causes of reproductive disorders in dairy cows in the Kembata Tambaro zone, southern Ethiopia","authors":"B. Ayele, S. Leta","doi":"10.1186/s44149-021-00014-8","DOIUrl":"https://doi.org/10.1186/s44149-021-00014-8","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41405778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-26DOI: 10.1186/s44149-021-00015-7
L. Yao, Chang Li, Junwei Wang, Yufang Cheng, Ahmed. H. Ghonaim, Qi Sun, Xuexiang Yu, Weijie Niu, S. Fan, Qigai He
{"title":"Development of an indirect immunofluorescence assay for PCV3 antibody detection based on capsid protein","authors":"L. Yao, Chang Li, Junwei Wang, Yufang Cheng, Ahmed. H. Ghonaim, Qi Sun, Xuexiang Yu, Weijie Niu, S. Fan, Qigai He","doi":"10.1186/s44149-021-00015-7","DOIUrl":"https://doi.org/10.1186/s44149-021-00015-7","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s44149-021-00015-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42712670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-18DOI: 10.1186/s44149-021-00007-7
D. Hu, Shaowen Li, Rendong Fang, H. Ono
{"title":"Update on molecular diversity and multipathogenicity of staphylococcal superantigen toxins","authors":"D. Hu, Shaowen Li, Rendong Fang, H. Ono","doi":"10.1186/s44149-021-00007-7","DOIUrl":"https://doi.org/10.1186/s44149-021-00007-7","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s44149-021-00007-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47126907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-23DOI: 10.1186/s44149-021-00001-z
Zhengwang Yu, Jie Huang, Zhongxin Zhou
{"title":"Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism","authors":"Zhengwang Yu, Jie Huang, Zhongxin Zhou","doi":"10.1186/s44149-021-00001-z","DOIUrl":"https://doi.org/10.1186/s44149-021-00001-z","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s44149-021-00001-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46560278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-23DOI: 10.1186/s44149-021-00002-y
G. Zhu, Jigang Yin, G. Cuny
{"title":"Current status and challenges in drug discovery against the globally important zoonotic cryptosporidiosis","authors":"G. Zhu, Jigang Yin, G. Cuny","doi":"10.1186/s44149-021-00002-y","DOIUrl":"https://doi.org/10.1186/s44149-021-00002-y","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s44149-021-00002-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49585067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Porcine epidemic diarrhea (PED) caused by the porcine epidemic diarrhea virus (PEDV), is a severe infectious and devastating swine disease that leads to serious economic losses in the swine industry worldwide. An increased number of PED cases caused by variant PEDV have been reported in many countries since 2010. S protein is the main immunogenic protein containing some B-cell epitopes that can induce neutralizing antibodies of PEDV. In this study, the construction, expression and purification of Pseudomonas aeruginosa exotoxin A (PE) without domain III (PEΔIII) as a vector was performed for the delivery of PEDV S-A or S-B. PE(ΔIII) PEDV S-A and PE(ΔIII) PEDV S-B recombinant proteins were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis. The immunogenicity of PEDV S-A and PEDV S-B subunit vaccines were evaluated in mice. The results showed that PEDV-S-B vaccine could not only induce specific humoral and Th1 type-dominant cellular immune responses, but also stimulate PEDV-specific mucosal immune responses in mice. PEDV-S-B subunit vaccine is a novel candidate mucosal vaccine against PEDV infection.
{"title":"Inactivated Pseudomonas PE(ΔIII) exotoxin fused to neutralizing epitopes of PEDV S proteins produces a specific immune response in mice.","authors":"Leqiang Sun, Yajie Tang, Keji Yan, Huanchun Chen, Huawei Zhang","doi":"10.1186/s44149-021-00021-9","DOIUrl":"https://doi.org/10.1186/s44149-021-00021-9","url":null,"abstract":"<p><p>Porcine epidemic diarrhea (PED) caused by the porcine epidemic diarrhea virus (PEDV), is a severe infectious and devastating swine disease that leads to serious economic losses in the swine industry worldwide. An increased number of PED cases caused by variant PEDV have been reported in many countries since 2010. S protein is the main immunogenic protein containing some B-cell epitopes that can induce neutralizing antibodies of PEDV. In this study, the construction, expression and purification of Pseudomonas aeruginosa exotoxin A (PE) without domain III (PEΔIII) as a vector was performed for the delivery of PEDV S-A or S-B. PE(ΔIII) PEDV S-A and PE(ΔIII) PEDV S-B recombinant proteins were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis. The immunogenicity of PEDV S-A and PEDV S-B subunit vaccines were evaluated in mice. The results showed that PEDV-S-B vaccine could not only induce specific humoral and Th1 type-dominant cellular immune responses, but also stimulate PEDV-specific mucosal immune responses in mice. PEDV-S-B subunit vaccine is a novel candidate mucosal vaccine against PEDV infection.</p>","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39892154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01Epub Date: 2021-11-26DOI: 10.1186/s44149-021-00026-4
Janine T Bossé, Yanwen Li, Leon G Leanse, Liqing Zhou, Roy R Chaudhuri, Sarah E Peters, Jinhong Wang, Gareth A Maglennon, Matthew T G Holden, Duncan J Maskell, Alexander W Tucker, Brendan W Wren, Andrew N Rycroft, Paul R Langford
Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide, and this transposon has been used for mutagenesis of a wide variety of bacteria. However, plasmids for mariner delivery do not necessarily work well in all bacteria. In particular, there are limited tools for functional genomic analysis of Pasteurellaceae species of major veterinary importance, such as swine and cattle pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, respectively. Here, we developed plasmids, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), that allow delivery of mariner into both these pathogens, but which should also be applicable to a wider range of bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and P. multocida that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS. A preliminary screen of 5000 mutants each identified 8 and 14 genes, respectively, that are required for growth under anaerobic conditions. Future high-throughput screening of the generated libraries will facilitate identification of mutants required for growth under different conditions, including in vivo, highlighting key virulence factors and pathways that can be exploited for development of novel therapeutics and vaccines.
{"title":"Rationally designed <i>mariner</i> vectors for functional genomic analysis of <i>Actinobacillus pleuropneumoniae</i> and other <i>Pasteurellaceae</i> species by transposon-directed insertion-site sequencing (TraDIS).","authors":"Janine T Bossé, Yanwen Li, Leon G Leanse, Liqing Zhou, Roy R Chaudhuri, Sarah E Peters, Jinhong Wang, Gareth A Maglennon, Matthew T G Holden, Duncan J Maskell, Alexander W Tucker, Brendan W Wren, Andrew N Rycroft, Paul R Langford","doi":"10.1186/s44149-021-00026-4","DOIUrl":"https://doi.org/10.1186/s44149-021-00026-4","url":null,"abstract":"<p><p>Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The <i>Himar1</i> (<i>mariner</i>) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide, and this transposon has been used for mutagenesis of a wide variety of bacteria. However, plasmids for <i>mariner</i> delivery do not necessarily work well in all bacteria. In particular, there are limited tools for functional genomic analysis of <i>Pasteurellaceae</i> species of major veterinary importance, such as swine and cattle pathogens, <i>Actinobacillus pleuropneumoniae</i> and <i>Pasteurella multocida</i>, respectively. Here, we developed plasmids, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), that allow delivery of <i>mariner</i> into both these pathogens, but which should also be applicable to a wider range of bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating <i>mariner</i> mutant libraries in both <i>A. pleuropneumoniae</i> and <i>P. multocida</i> that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS. A preliminary screen of 5000 mutants each identified 8 and 14 genes, respectively, that are required for growth under anaerobic conditions. Future high-throughput screening of the generated libraries will facilitate identification of mutants required for growth under different conditions, including <i>in vivo</i>, highlighting key virulence factors and pathways that can be exploited for development of novel therapeutics and vaccines.</p>","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39809410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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