{"title":"Isolation, Molecular Characterization and Growth Promoting Activity of Free Living Diazotrophs Screened from Soils of Lesotho","authors":"P. C. Monokoane, S. Mekbib, Liu Haiying","doi":"10.15413/AJAR.2015.0214","DOIUrl":"https://doi.org/10.15413/AJAR.2015.0214","url":null,"abstract":"","PeriodicalId":7053,"journal":{"name":"Academia Journal of Agricultural Research","volume":"33 1","pages":"45-52"},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73997311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. W. Mukhongo, Agnes Mumo Kavoo-Mwangi, J. R. Okalebo, B. A. Were, E. Mwangi, J. Jefwa
The efficacy of co-inoculation of microbiological products and combining them with a chemical stimulant in enhancing survival and growth of tissue culture banana under different soil conditions was investigated in the greenhouse. Tissue cultured banana (Gros Mitchel cv.) was inoculated with products containing Bacillus spp., arbuscular mycorrhizal fungi (AMF), Trichoderma harzianum, Myconate (chemical stimulant) and their combinations. Two soil types (Rhodic Ferralsol and Vertisol) were used at the hardening and potting phases and inoculation of plants was done at both phases. Plant growth was assessed at an interval of two weeks. Destructive harvesting was done and mycorrhizal colonization, root and shoot biomass and shoot nutrient uptake were assessed at the end of nursery phase. The effect of the products on the measured parameters depended on soil type with Vertisol being most receptive to inoculation. The combination of Bacillus spp. with AMF or Myconate or T. harzianum in the Vertisol gave the most significant increase in plant growth by over 28, 24 and 14%, respectively and in plant biomass accumulation by over 34, 46 and 33%, respectively compared to the control. Mycorrhizal colonization was not significantly affected by product inoculation in the two soils. In the Rhodic Ferralsol, the combination of arbuscular mycorrhizal fungi with T. harzianum promoted the highest uptake of zinc, boron, magnesium and phosphorus; T. harzianum+ Bacillus spp. promoted the highest uptake of potassium, while AMF+ Myconate promoted the highest uptake of calcium. In the Vertisol, the combination of Bacillus spp. with arbuscular mycorrhiza fungi gave the highest uptake of phosphorus, magnesium, calcium and boron; Bacillus spp.+ Myconate gave the highest uptake of potassium, while singly applied AMF gave the highest uptake of zinc. Results demonstrate that tissue cultured bananas’ survival and growth can benefit from co-inoculation of arbuscular mycorrhizal fungi and Trichoderma or Bacillus or a chemical stimulant during the nursery phase. The effect of coinoculation is however depended on soil type.
{"title":"Acclimatization and growth of tissue cultured banana co-inoculated with microbiological and chemical commercial products in different soils in Kenya","authors":"R. W. Mukhongo, Agnes Mumo Kavoo-Mwangi, J. R. Okalebo, B. A. Were, E. Mwangi, J. Jefwa","doi":"10.15413/ajar.2015.0113","DOIUrl":"https://doi.org/10.15413/ajar.2015.0113","url":null,"abstract":"The efficacy of co-inoculation of microbiological products and combining them with a chemical stimulant in enhancing survival and growth of tissue culture banana under different soil conditions was investigated in the greenhouse. Tissue cultured banana (Gros Mitchel cv.) was inoculated with products containing Bacillus spp., arbuscular mycorrhizal fungi (AMF), Trichoderma harzianum, Myconate (chemical stimulant) and their combinations. Two soil types (Rhodic Ferralsol and Vertisol) were used at the hardening and potting phases and inoculation of plants was done at both phases. Plant growth was assessed at an interval of two weeks. Destructive harvesting was done and mycorrhizal colonization, root and shoot biomass and shoot nutrient uptake were assessed at the end of nursery phase. The effect of the products on the measured parameters depended on soil type with Vertisol being most receptive to inoculation. The combination of Bacillus spp. with AMF or Myconate or T. harzianum in the Vertisol gave the most significant increase in plant growth by over 28, 24 and 14%, respectively and in plant biomass accumulation by over 34, 46 and 33%, respectively compared to the control. Mycorrhizal colonization was not significantly affected by product inoculation in the two soils. In the Rhodic Ferralsol, the combination of arbuscular mycorrhizal fungi with T. harzianum promoted the highest uptake of zinc, boron, magnesium and phosphorus; T. harzianum+ Bacillus spp. promoted the highest uptake of potassium, while AMF+ Myconate promoted the highest uptake of calcium. In the Vertisol, the combination of Bacillus spp. with arbuscular mycorrhiza fungi gave the highest uptake of phosphorus, magnesium, calcium and boron; Bacillus spp.+ Myconate gave the highest uptake of potassium, while singly applied AMF gave the highest uptake of zinc. Results demonstrate that tissue cultured bananas’ survival and growth can benefit from co-inoculation of arbuscular mycorrhizal fungi and Trichoderma or Bacillus or a chemical stimulant during the nursery phase. The effect of coinoculation is however depended on soil type.","PeriodicalId":7053,"journal":{"name":"Academia Journal of Agricultural Research","volume":"64 1","pages":"156-168"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79043625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-02-01DOI: 10.3844/ajessp.2014.123.128
N. Al-Rawashdeh
Organic farming is considered as a mitigation strat egy to face adverse effect of climate change and Consumers’ increasing their need for safe and quali ty food has motivated this research study. The oliv e oil of the Roman olive trees (Landrace) Olea Europea belong to the Oleaceae family growing under organic farming (in the transition period) conditions in Jordan Ajl un area was extracted by traditional method; warming water to the about 60°C and grounded the seeds make as paste then pour in the warmed water then take the oil layer floated at the surface. The oil was analyzed for fa tty acids pattern and compared with Romans trees ol ive oil grown at Burma Agriculture Station which has organic Certificate according to the Japanese Agriculture Standard (JAS) since 2007. The separation patterns of fatty acids were done by Gas Chromatography (GC). Results showed high levels of unsaturated fatty aci ds, particularly oleic acid (up to 67.49%), linolei c acid (13.31%) and linolenic acid (0.74%) in the oil of R omans trees growing in Ajlun area, while the organi c olive oil from the Romans olive trees growing at Burma Station showed 68.88% of oleic acid, 11.73% of linole ic acid and 0.67% of Linolenic acid. The analysis also showed that the palmatic saturated fatty acid was 12.54% at Burma Station compared to 11.82% in Ajlun area. The medicinal value of unsaturated fatty acids play great role for reducing cholesterol rate that was found i n high level of Romans olive trees (landrace).
{"title":"Fatty acids pattern of olive oil under organic farming.","authors":"N. Al-Rawashdeh","doi":"10.3844/ajessp.2014.123.128","DOIUrl":"https://doi.org/10.3844/ajessp.2014.123.128","url":null,"abstract":"Organic farming is considered as a mitigation strat egy to face adverse effect of climate change and Consumers’ increasing their need for safe and quali ty food has motivated this research study. The oliv e oil of the Roman olive trees (Landrace) Olea Europea belong to the Oleaceae family growing under organic farming (in the transition period) conditions in Jordan Ajl un area was extracted by traditional method; warming water to the about 60°C and grounded the seeds make as paste then pour in the warmed water then take the oil layer floated at the surface. The oil was analyzed for fa tty acids pattern and compared with Romans trees ol ive oil grown at Burma Agriculture Station which has organic Certificate according to the Japanese Agriculture Standard (JAS) since 2007. The separation patterns of fatty acids were done by Gas Chromatography (GC). Results showed high levels of unsaturated fatty aci ds, particularly oleic acid (up to 67.49%), linolei c acid (13.31%) and linolenic acid (0.74%) in the oil of R omans trees growing in Ajlun area, while the organi c olive oil from the Romans olive trees growing at Burma Station showed 68.88% of oleic acid, 11.73% of linole ic acid and 0.67% of Linolenic acid. The analysis also showed that the palmatic saturated fatty acid was 12.54% at Burma Station compared to 11.82% in Ajlun area. The medicinal value of unsaturated fatty acids play great role for reducing cholesterol rate that was found i n high level of Romans olive trees (landrace).","PeriodicalId":7053,"journal":{"name":"Academia Journal of Agricultural Research","volume":"70 1","pages":"156-160"},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85584311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Abolusoro, P. Abolusoro, F. Mathew, N. B. Izuogu
Pot experiment was conducted twice with a sterilized soil to evaluate the effects of organic manures (poultry, cow dung, domestic waste) and inorganic manure (NPK 15 : 15 : 15) on the growth attributes of root-knot nematode infected Ethopian egg plant Solanum aethiopicum in a screen house at Kabba College of Agriculture, Ahmadu Bello University Kabba, Nigeria. Each of the organic manure was applied at the rate of 5t/ha and the NPK fertilizer was applied at the rate of 200kg/ha, while there was an uncreated control which acted as standard check. The experiment was designed in a completely randomized design comprising of five treatments as earlier mentioned and each treatment was replicated four times. The result of the experiment shows that all the manures were effective in suppressing nematode activities as manifested in enhanced growth observed in both organic and inorganic manure treatments compared with the control. The mean plant height, number of leaves, stem girth and number of branches in organic and inorganic manure root-knot nematode infected Ethopian egg plant was of the range 46.5 ± 3.18, 19 ± 3, 1.24 ± 6 and 6 ± 1 respectively at the twelve week of the experiment while the order of 16.83, 11.33, 0.80 and 2.67 respectively were observed in control plants. Egg plants in control treatment were heavily galled by Meloidogyne incognita resulting in poor growth while the organic and inorganic manure treated egg plant recorded better growth and were significantly different from the control. This result suggests the use of organic manure as soil amendment for the control of root-knot nematode in the endemic soil where susceptible crop are to be grown.
{"title":"Effects of organic and inorganic manures on the growth attributes of root-knot nematode (Meloidogyne incognita) infected Ethiopian egg plant (Solanum aethiopicum).","authors":"S. Abolusoro, P. Abolusoro, F. Mathew, N. B. Izuogu","doi":"10.12691/WJAR-1-6-2","DOIUrl":"https://doi.org/10.12691/WJAR-1-6-2","url":null,"abstract":"Pot experiment was conducted twice with a sterilized soil to evaluate the effects of organic manures (poultry, cow dung, domestic waste) and inorganic manure (NPK 15 : 15 : 15) on the growth attributes of root-knot nematode infected Ethopian egg plant Solanum aethiopicum in a screen house at Kabba College of Agriculture, Ahmadu Bello University Kabba, Nigeria. Each of the organic manure was applied at the rate of 5t/ha and the NPK fertilizer was applied at the rate of 200kg/ha, while there was an uncreated control which acted as standard check. The experiment was designed in a completely randomized design comprising of five treatments as earlier mentioned and each treatment was replicated four times. The result of the experiment shows that all the manures were effective in suppressing nematode activities as manifested in enhanced growth observed in both organic and inorganic manure treatments compared with the control. The mean plant height, number of leaves, stem girth and number of branches in organic and inorganic manure root-knot nematode infected Ethopian egg plant was of the range 46.5 ± 3.18, 19 ± 3, 1.24 ± 6 and 6 ± 1 respectively at the twelve week of the experiment while the order of 16.83, 11.33, 0.80 and 2.67 respectively were observed in control plants. Egg plants in control treatment were heavily galled by Meloidogyne incognita resulting in poor growth while the organic and inorganic manure treated egg plant recorded better growth and were significantly different from the control. This result suggests the use of organic manure as soil amendment for the control of root-knot nematode in the endemic soil where susceptible crop are to be grown.","PeriodicalId":7053,"journal":{"name":"Academia Journal of Agricultural Research","volume":"6 2 1","pages":"083-087"},"PeriodicalIF":0.0,"publicationDate":"2013-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78335616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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