Archives of clinical & experimental dermatology最新文献

{"title":"Macrophage Response to Simulated Solar Radiation in the Development of Human Malignant Melanoma.","authors":"Sherman Chu,&nbsp;Tatyana A Petukhova,&nbsp;Jeremy S Bordeaux,&nbsp;Thomas S McCormick,&nbsp;Kevin D Cooper","doi":"10.46527/2583-6374.119","DOIUrl":"https://doi.org/10.46527/2583-6374.119","url":null,"abstract":"<p><strong>Background: </strong>IFN-γ is widely debated regarding its purported anti- or pro-tumorigenic properties. We initiated a pilot study of primary malignant melanoma patients to investigate whether macrophage-derived IFN-γ is produced in humans as proposed in murine melanomagenesis models.</p><p><strong>Methods: </strong>Biopsy specimens of fresh-frozen primary melanoma tissue were used to quantify co-localization of IFN-γ, macrophages, lymphocytes, and downstream IFN-γ signatures. Additionally, we analyzed simulated solar radiation (SSR) exposed skin in patients with a history of melanoma versus healthy controls to compare the relative magnitude of macrophage infiltration.</p><p><strong>Results: </strong>Our data identified a subset of tumor infiltrating CD68⁺ macrophages that co-localized with IFN-γ (Pearson's Correlation = 0.33 ± 0.11) in patients with primary melanoma (Stage 0-III). Additionally, a population of infiltrating CD3⁺ lymphocytes strongly co-localized with IFN-γ (Pearson's Correlation = 0.57 ± 0.11). Malignant melanoma cells were double positive for downstream IFN-γ response elements, MIG/CXCL9, and phosphorylated STAT-1 (P-STAT-1). Cellular signaling pathways were also observed when we exposed the skin of melanoma patients to SSR. Despite robust CXCL9 expression in the epidermis of SSR-exposed skin of melanoma patients, we observed decreased macrophage infiltration into melanoma patient skin.</p><p><strong>Conclusion: </strong>Peritumoral macrophages in melanoma patient skin produce IFN-γ and melanocytes appear to exhibit in vivo responsiveness to IFN-γ, such as P-STAT-1 and upregulated CXCL9 expression. However, despite producing CXCL9 in response to SSR, the normal skin of melanoma patients demonstrates a weak leukocyte infiltration. Immune-modulatory studies for the prevention or treatment of human malignant melanoma may need to address complex tissue and melanocyte signaling and crosstalk.</p>","PeriodicalId":72278,"journal":{"name":"Archives of clinical & experimental dermatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10259476/pdf/nihms-1873545.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9707539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Basal Cell Carcinoma Developed on an Active Lesion of Mucocutaneous Leishmaniasis: A Case Report from Yemen","authors":"Mohamed A Al-Kamel","doi":"10.24983/SCITEMED.ACD.2018.00072","DOIUrl":"https://doi.org/10.24983/SCITEMED.ACD.2018.00072","url":null,"abstract":"The author presents a case of BCC developed on an active lesion of mucocutaneous leishmaniasis as one of many cases of skin cancer frequently seen at the Regional Leishmaniasis Control Center (RLCC) clinics in Yemen.","PeriodicalId":72278,"journal":{"name":"Archives of clinical & experimental dermatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79640189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rethinking of Small Molecules in Treating Psoriasis","authors":"Longgui Wang","doi":"10.24983/scitemed.acd.2017.00042","DOIUrl":"https://doi.org/10.24983/scitemed.acd.2017.00042","url":null,"abstract":"The author concerns that long-term application of biologics will destroy the natural balance, and argues to rethink about small molecules that can restore immune homeostasis, thereby naturally correcting overproduced proinflammatory cytokines, as a strategy of new drug development against psoriasis and other autoimmune disorders.","PeriodicalId":72278,"journal":{"name":"Archives of clinical & experimental dermatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88435155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alopecia Areata and Severe Psoriasis Successfully Treated with Apremilast","authors":"S. López, C. D. Castro, Pablo Munguía Calzada, P. Segura","doi":"10.24983/SCITEMED.ACD.2017.00033","DOIUrl":"https://doi.org/10.24983/SCITEMED.ACD.2017.00033","url":null,"abstract":"This case report shows the effectiveness of Apremilast in refractory ophiasis AA with a rapid and highly scalp-hair regrowth with a cosmetically important response.","PeriodicalId":72278,"journal":{"name":"Archives of clinical & experimental dermatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82559929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Room Temperature Saline-soaked Gauze Assists in Hemostasis Between Mohs Layers","authors":"L. Sutton, I. Orengo","doi":"10.24983/SCITEMED.ACD.2017.00027","DOIUrl":"https://doi.org/10.24983/SCITEMED.ACD.2017.00027","url":null,"abstract":"leeding between Mohs layers is distressing to the patient and potentially time-consuming for the physician. Electrosurgery is utilized for hemostasis in most Mohs cases. Obtaining complete hemostasis between Mohs layers can be difficult as anticoagulant use by patients is often encountered. We present a simple and cost-effective method to assist in obtaining hemostasis between Mohs layers. Advantages of this method include quick hemostasis that doesn’t require significant electrodesiccation; therefore, saves time for the physician between Mohs layers, less destruction of tissue that may be critical for the reconstruction, and perhaps decreases exposure to smoke from electrosurgery. We have found the use of gauze soaked with room temperature sterile saline and then applied to the wound bed under a pressure bandage can assist in hemostasis. We use room temperature saline which is most commonly around 20-25°C. We presume the success of this method is due to the saline being cooler than body temperature; therefore, precipitating local vasoconstriction. The saline-weighted gauze might also provide additional pressure. This technique is particularly useful for eyelid lesions where minimizing electrosurgery to improve outcome is critical. Other locations that seem to benefit from this technique are scalp and nose defects. Although we have found success with this method repeatedly, the literature is divergent regarding the temperature of saline to assist in hemostasis. Perhaps contrary to intuition, warm saline opposed to cool or room temperature saline has been associated with reduced bleeding [1]. A recent study in oral surgery compared dressings soaked in room temperature saline compared to saline dressings warmed to 42°C. Significantly reduced bleeding was reported in this split-mouth study with the warmed saline dressings. These findings are aligned with results previously reported in endoscopic sinus surgery and treatment of epistaxis. The use of irrigation with warmed water for epistaxis and minor vessel hemostasis in endoscopic sinus surgery has been reported [2-4]. There is no literature looking at warm soaks on cutaneous versus mucosal surfaces. It has been hypothesized that hypothermia inhibits enzymatic reactions of the coagulation cascade and might impair platelet reactivity [5]. Hypothermia from general anesthesia has been associated with coagulopathy [6]. By increasing the body temperature, this coagulopathy might be nullified and actually reversed. In addition, other mechanisms of action proposed include interstitial edema precipitating compression on blood vessels [7]. Vasodilation of the vessels is also thought to decrease intraluminal pressure that would slow blood flow. Under this theory, the role of vasoconstriction due to hypothermia contributing to hemostasis has yet to be determined and would play a secondary role. The use of cold saline has been supported in various literature. A randomized, control trial during total knee arthro","PeriodicalId":72278,"journal":{"name":"Archives of clinical & experimental dermatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86359551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}