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Isolation of nine novel tetranucleotide microsatellites in Atlantic herring (Clupea harengus) 大西洋鲱鱼9个新型四核苷酸微卫星的分离
Pub Date : 2001-03-01 DOI: 10.1046/J.1471-8278
Arran A. McPherson, P. O’Reilly, T. McParland, M. Jones, P. Bentzen
Nine tetranucleotide microsatellite loci developed for Atlantic herring are presented. Loci were isolated using a magnetic bead hybridization selection protocol that yielded an 80% enrichment for tetranucleotide microsatellites. Fifty Atlantic herring from each of a North-west and North-east Atlantic spawning group were screened at each locus. Loci were polymorphic (12–56 alleles per locus) and exhibited high levels of observed heterozygosity (0.66–0.98). These loci are therefore suitable for population studies and the analysis of mating systems.
报道了大西洋鲱鱼的9个四核苷酸微卫星位点。使用磁珠杂交选择方案分离位点,获得80%的四核苷酸微卫星富集。来自西北和东北大西洋产卵群各50条大西洋鲱鱼在每个地点进行了筛选。这些位点具有多态性(每个位点有12-56个等位基因),并表现出较高的杂合度(0.66-0.98)。因此,这些基因座适合用于种群研究和交配系统分析。
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引用次数: 28
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Molecular ecology notes
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