Pub Date : 2021-02-12DOI: 10.9734/BPI/CACB/V2/7213D
Pankaj Kumar, Kushagra Jain, S. Munilkumar, V.Phulia R.S.Chalal
A 60-days feeding trial was conducted to study the effect of different feeding strategies with normal and low protein diet on digestive and metabolic activity in Labeo rohita fingerlings. One hundred thirty five fingerlings were distributed in triplicate groups of each treatment. Three experimental isocaloric (401.32 to 410.28 kcal/100g) diets of 30%, 25% and 20% crude protein designated as dietA, diet B and diet C respectively were prepared, using locally available feed ingredients. Three different feeding schedules of normal protein diet continuously (diet A-30%) throughout the experiment, alternate feeding of 1-day dietAfollowed by 1-day dietB (1A/1B) and alternate feeding of 1-day dietAfollowed by 1-day diet C (1A/1C) throughout the experiment were tested by feeding the fishes at 5% body weight daily. Results showed that digestive enzymes activity such as protease and amylasewas higher in the group fed 1A/1B.Glucose 6 phosphatase (G6Pase) were also analysed. The metabolic enzymes such as lactate dehydrogenase (LDH), malate dehydrogenase (MDH) are reported to be significantly difference (p<0.05) in among the different feeding schedules. The proteolytic activity such as alanine amino transaminase (ALT), aspartate amino transaminase (AST), alkaline phosphatise (ALP) were reported to be higher in the group fed with 1A/1B feeding schedule.
{"title":"Research on Responses of Digestive and Metabolic Enzymes to Different Feeding Strategies with Sub-optimal Protein Level in Labeo rohita Fingerlings","authors":"Pankaj Kumar, Kushagra Jain, S. Munilkumar, V.Phulia R.S.Chalal","doi":"10.9734/BPI/CACB/V2/7213D","DOIUrl":"https://doi.org/10.9734/BPI/CACB/V2/7213D","url":null,"abstract":"A 60-days feeding trial was conducted to study the effect of different feeding strategies with normal and low protein diet on digestive and metabolic activity in Labeo rohita fingerlings. One hundred thirty five fingerlings were distributed in triplicate groups of each treatment. Three experimental isocaloric (401.32 to 410.28 kcal/100g) diets of 30%, 25% and 20% crude protein designated as dietA, diet B and diet C respectively were prepared, using locally available feed ingredients. Three different feeding schedules of normal protein diet continuously (diet A-30%) throughout the experiment, alternate feeding of 1-day dietAfollowed by 1-day dietB (1A/1B) and alternate feeding of 1-day dietAfollowed by 1-day diet C (1A/1C) throughout the experiment were tested by feeding the fishes at 5% body weight daily. Results showed that digestive enzymes activity such as protease and amylasewas higher in the group fed 1A/1B.Glucose 6 phosphatase (G6Pase) were also analysed. The metabolic enzymes such as lactate dehydrogenase (LDH), malate dehydrogenase (MDH) are reported to be significantly difference (p<0.05) in among the different feeding schedules. The proteolytic activity such as alanine amino transaminase (ALT), aspartate amino transaminase (AST), alkaline phosphatise (ALP) were reported to be higher in the group fed with 1A/1B feeding schedule.","PeriodicalId":8793,"journal":{"name":"BioChemistry: An Indian Journal","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74660211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: Chenopodium quinoa, an ancient crop sprung initially in Andean region of South America is well recognized for its outstanding nutritional composition and versatility. Quinoa from various geographical regions like Bolivia, Argentina, Kenya, Japan and Morocco has been studied, however there is no study on Indian Chenopodium quinoa. Thus, the present study aims to determine vitamin C, antioxidant activity and total polyphenols in Indian quinoa. Also, it compares effect of domestic and industrial processing on the grain. RESULTS: The results show that domestically processed seeds have higher vitamin C, total phenolic content (TPC), total flavonoid content (TFC) and also antioxidant activity as compared to the raw and industrially processed seeds. Antioxidant activity was found significantly correlated to the total phenolic content in raw, domestically processed and industrially processed seeds. CONCLUSION: Domestically processed quinoa, mainly by germination is reported to be rich in antioxidants, vitamin C and higher phenolic content. The results suggest use of domestic processing of quinoa seeds to retain nutrient value and also infer dietary importance of Indian Chenopodium quinoa. Further studies are required on antioxidant and polyphenol profiling of Indian quinoa.
{"title":"Vitamin C, total polyphenols and antioxidant activity in raw, domestically processed and industrially processed Indian Chenopodium quinoa seeds","authors":"Intelli, D. Reddy, Ambika Chauhan","doi":"10.7324/JAPS.2016.60419","DOIUrl":"https://doi.org/10.7324/JAPS.2016.60419","url":null,"abstract":"BACKGROUND: Chenopodium quinoa, an ancient crop sprung initially in Andean region of South America is well recognized for its outstanding nutritional composition and versatility. Quinoa from various geographical regions like Bolivia, Argentina, Kenya, Japan and Morocco has been studied, however there is no study on Indian Chenopodium quinoa. Thus, the present study aims to determine vitamin C, antioxidant activity and total polyphenols in Indian quinoa. Also, it compares effect of domestic and industrial processing on the grain. RESULTS: The results show that domestically processed seeds have higher vitamin C, total phenolic content (TPC), total flavonoid content (TFC) and also antioxidant activity as compared to the raw and industrially processed seeds. Antioxidant activity was found significantly correlated to the total phenolic content in raw, domestically processed and industrially processed seeds. CONCLUSION: Domestically processed quinoa, mainly by germination is reported to be rich in antioxidants, vitamin C and higher phenolic content. The results suggest use of domestic processing of quinoa seeds to retain nutrient value and also infer dietary importance of Indian Chenopodium quinoa. Further studies are required on antioxidant and polyphenol profiling of Indian quinoa.","PeriodicalId":8793,"journal":{"name":"BioChemistry: An Indian Journal","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81086139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Renin-Angiotensin system (RAS) is a key regulator of both blood pressure and kidney functions and their interaction. In such a situation, genetic variability in the genes of different components of RAS is likely to contribute for its heterogeneous association in the renal disease patients. Angiotensin converting enzyme-1 (ACE-1) is an important component of RASwhich determines the vasoactive peptideAngiotensin-II. In the present study,we have investigated 103 end stage renal diseases (ESRD), 104 primary nephrotic (P.N) patients and 102 normal healthy controls from Mansoura city in Egypt to deduce the association between ACE gene polymorphism and ESRD, P.N. The selected sampleswere assayed for genotyping ofACE I/D by (PCR) based DNAamplification using specific flanking primers. The results revealed that there was a significance distribution in DD genotype between ESRD and control group (p 1) which resulting in increasing the risk for ESRD. Therewas significance distribution in ID genotype between ESRD and control group (p<0.05), without disease risk (OR <1). Based on these observations we conclude that ACE DD genotype implicate a strong possible role in the in renal damage among Egyptians. The studywill help in predetermining the timing, type and doses of therapy for ESRD patients.
{"title":"Angiotensin converting enzyme genotype prevalence among Egyptian primary nephrotic and end stage renal diseases patients","authors":"F. Zahran, S.Hamed, A.T.Keshta, M.Hussien","doi":"10.21608/BLJ.2012.64269","DOIUrl":"https://doi.org/10.21608/BLJ.2012.64269","url":null,"abstract":"The Renin-Angiotensin system (RAS) is a key regulator of both blood pressure and kidney functions and their interaction. In such a situation, genetic variability in the genes of different components of RAS is likely to contribute for its heterogeneous association in the renal disease patients. Angiotensin converting enzyme-1 (ACE-1) is an important component of RASwhich determines the vasoactive peptideAngiotensin-II. In the present study,we have investigated 103 end stage renal diseases (ESRD), 104 primary nephrotic (P.N) patients and 102 normal healthy controls from Mansoura city in Egypt to deduce the association between ACE gene polymorphism and ESRD, P.N. The selected sampleswere assayed for genotyping ofACE I/D by (PCR) based DNAamplification using specific flanking primers. The results revealed that there was a significance distribution in DD genotype between ESRD and control group (p 1) which resulting in increasing the risk for ESRD. Therewas significance distribution in ID genotype between ESRD and control group (p<0.05), without disease risk (OR <1). Based on these observations we conclude that ACE DD genotype implicate a strong possible role in the in renal damage among Egyptians. The studywill help in predetermining the timing, type and doses of therapy for ESRD patients.","PeriodicalId":8793,"journal":{"name":"BioChemistry: An Indian Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73473505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-02-01DOI: 10.5251/ABJNA.2011.2.2.197.202
Baghizadeh, Amin, Yazdanpanah Ali, K. Sahar
Study of the genetic diversity in plant inherited stores in order to classify the germplasm regarding resistance to biotic and biotic stress and also preventing from genetic erosion is one of the basic and fundamental steps in the most breeding programs .Iran is one of the most important centers of genetic diversity due to having different climates and the old civilization. In order to distinguish and evaluate different genetic of aegilops germplasm, which were collected fromwest part of the Iran, 80 ecotypes of aegilops by RAPDmolecularmarkerwere assessed .DNAextractionwas done bymodified CTAB method.After DNAextraction stages, complement gene locuses were amplified by 18 RAPD primers. These primers produced 183 bands, that 151 bands (about 82%) were polymorphic. Cluster analysis based on the resulting datawas performed usingUPGMAmethod and DiceÂÂÂs similarity coefficient in NTSYS software. The resulting dendogram categorized the accessions into 8 groups in 69%similarity. Principle ComponentAnalysis (PCA) was performed too, 2 and 3 dimensions graphs using 18 primers were drawn.
{"title":"Genetic diversity assessment of Aegilops germplasm by RAPD molecular markers","authors":"Baghizadeh, Amin, Yazdanpanah Ali, K. Sahar","doi":"10.5251/ABJNA.2011.2.2.197.202","DOIUrl":"https://doi.org/10.5251/ABJNA.2011.2.2.197.202","url":null,"abstract":"Study of the genetic diversity in plant inherited stores in order to classify the germplasm regarding resistance to biotic and biotic stress and also preventing from genetic erosion is one of the basic and fundamental steps in the most breeding programs .Iran is one of the most important centers of genetic diversity due to having different climates and the old civilization. In order to distinguish and evaluate different genetic of aegilops germplasm, which were collected fromwest part of the Iran, 80 ecotypes of aegilops by RAPDmolecularmarkerwere assessed .DNAextractionwas done bymodified CTAB method.After DNAextraction stages, complement gene locuses were amplified by 18 RAPD primers. These primers produced 183 bands, that 151 bands (about 82%) were polymorphic. Cluster analysis based on the resulting datawas performed usingUPGMAmethod and DiceÂÂÂs similarity coefficient in NTSYS software. The resulting dendogram categorized the accessions into 8 groups in 69%similarity. Principle ComponentAnalysis (PCA) was performed too, 2 and 3 dimensions graphs using 18 primers were drawn.","PeriodicalId":8793,"journal":{"name":"BioChemistry: An Indian Journal","volume":"479 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86757309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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