Cindy R Cisar, Tatsuya Akiyama, Jonathan Hatley, Lori Arney, Nebojsa Kezunovic, Daniel Owen
Bacteroidales are fecal anaerobic bacteria that are common in the digestive systems and feces of warm-blooded animals. Some strains of Bacteroidales have been reported to be host-specific. In this study, Bacteroidales strains from chicken feces were examined for their potential use as indicators of chicken fecal contamination. Bacteroidales 16S rRNA gene sequences from chicken feces were amplified, cloned and sequenced. Phylogenetic analysis was performed using these sequences and published Bacteroidales 16S rRNA gene sequences from human and bovine feces. Primers were designed based on putative chicken feces-specific 16S rRNA gene sequences and the primer pairs were tested for specificity in PCR assays. One set of primers, chBact F1 and chBact R16, specifically amplified DNA from chicken feces in a PCR assay, but did not amplify wild turkey, cat, bovine, or deer fecal DNAs. In addition, DNA from feces contaminated straw-based chicken litter produced a product in the PCR assay. However, DNA from feces contaminated wood shavings-based chicken litter was not amplified. The PCR assay described here may prove a useful tool for the detection of chicken feces and for source tracking in watersheds with fecal contamination.
{"title":"PCR Assay Specific for Chicken Feces.","authors":"Cindy R Cisar, Tatsuya Akiyama, Jonathan Hatley, Lori Arney, Nebojsa Kezunovic, Daniel Owen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p><i>Bacteroidales</i> are fecal anaerobic bacteria that are common in the digestive systems and feces of warm-blooded animals. Some strains of <i>Bacteroidales</i> have been reported to be host-specific. In this study, <i>Bacteroidales</i> strains from chicken feces were examined for their potential use as indicators of chicken fecal contamination. <i>Bacteroidales</i> 16S rRNA gene sequences from chicken feces were amplified, cloned and sequenced. Phylogenetic analysis was performed using these sequences and published <i>Bacteroidales</i> 16S rRNA gene sequences from human and bovine feces. Primers were designed based on putative chicken feces-specific 16S rRNA gene sequences and the primer pairs were tested for specificity in PCR assays. One set of primers, chBact F1 and chBact R16, specifically amplified DNA from chicken feces in a PCR assay, but did not amplify wild turkey, cat, bovine, or deer fecal DNAs. In addition, DNA from feces contaminated straw-based chicken litter produced a product in the PCR assay. However, DNA from feces contaminated wood shavings-based chicken litter was not amplified. The PCR assay described here may prove a useful tool for the detection of chicken feces and for source tracking in watersheds with fecal contamination.</p>","PeriodicalId":90298,"journal":{"name":"Proceedings of the Oklahoma Academy of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022594/pdf/nihms-305832.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32350384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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