The in vitro effect of infrared laser light on the isomerization of all-trans retinal dissolved in an ether/hexane and also an ethanol solvent was studied. Pulsed laser energy at 1064 nm was used to drive the molecular reconfiguration of all-trans retinal to 11-cis retinal. High pressure liquid chromatography (HPLC) was used to quantify the conversion. Overall isomerization was minimal (0.2 percent to 1.0 percent), yet, a significant difference in isomerization due to pulsed infrared laser energy over non-modulated monochromatic laser light was detected (up to 168 percent difference). Potentially, pulsed laser radiation tuned to the ethylenic stretch frequency of the C11=C12 bond of retinal may induce rotational changes to the chromophore.
{"title":"Effects of Infrared Laser Radiation on the <i>In Vitro</i> Isomerization of All-<i>Trans</i> Retinal to 11-<i>Cis</i> Retinal.","authors":"J Liegner, J Taboada, A T C Tsin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The <i>in vitro</i> effect of infrared laser light on the isomerization of all-<i>trans</i> retinal dissolved in an ether/hexane and also an ethanol solvent was studied. Pulsed laser energy at 1064 nm was used to drive the molecular reconfiguration of all-<i>trans</i> retinal to 11-<i>cis</i> retinal. High pressure liquid chromatography (HPLC) was used to quantify the conversion. Overall isomerization was minimal (0.2 percent to 1.0 percent), yet, a significant difference in isomerization due to pulsed infrared laser energy over non-modulated monochromatic laser light was detected (up to 168 percent difference). Potentially, pulsed laser radiation tuned to the ethylenic stretch frequency of the C<sub>11</sub>=C<sub>12</sub> bond of retinal may induce rotational changes to the chromophore.</p>","PeriodicalId":91138,"journal":{"name":"Lasers in the life sciences","volume":"2 2","pages":"103-112"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550224/pdf/nihms588264.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33964339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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