Common bean root rot caused by different fungal pathogens is an important disease affecting common bean (Phaseolus vulgaris L.) production and productivity. In Ethiopia, this disease has become one of the most destructive biotic constraints to common bean production. Information on common bean root rot disease management is lacking for the Ethiopian common bean production system in general and West Hararghe in particular. Therefore, in the 2018 main cropping season, a field experiment was performed on 19 common bean varieties in two research fields, the ‘Bareda and Oda Baso’ farmers’ training centers. The experiment was designed to evaluate the level of resistance of a common bean genotype against root rot complex pathogens under natural conditions using a Randomized Complete Block Design (RCBD) design with three replications. The levels of resistance were evaluated based on seedling emergence, disease incidence, and severity in parallel with grain yield data and subjected to analysis of variance (ANOVA). Based on the laboratory results, four pathogenic fungi, namely, Fusarium oxysporum f. sp. Phaseoli (F. o. f. sp. Phaseoli), Rhizoctonia solani (R. soni), Sclerotium rolfsii (S. rolfsii), and Macrophomina phaseolina (M. phaseolina), a Nematode sp., and stem maggot insect were identified. The combined analysis of variance showed very highly significant variation at (p < 0.0001) across the location. There was also very highly significant variation (p < 0.0001) among treatments except for seedling emergence, incidence, and severity, which were highly significant (p < 0.001). From the experiment, seven common bean varieties (Kufanzik, Roba, Hirna, SeR-125, Cranscope, Tinike, Awash-1) that showed resistance characteristics and two susceptible varieties (Choire and Argane) were selected and recommended.
普通豆根腐病是由不同病原菌引起的一种影响普通豆生产和生产力的重要病害。在埃塞俄比亚，这种疾病已成为普通豆生产中最具破坏性的生物限制因素之一。一般而言，埃塞俄比亚普通豆生产系统，特别是西哈拉尔河地区，缺乏有关普通豆根腐病管理的信息。因此，在2018年主要种植季，在“Bareda”和“Oda Baso”农民培训中心两个研究区对19个普通豆类品种进行了田间试验。本试验采用随机完全区组设计(RCBD)，采用3个重复设计，评价普通大豆基因型在自然条件下对根腐病复合病原菌的抗性水平。抗性水平根据出苗率、发病率和严重程度与粮食产量数据进行评估，并进行方差分析(ANOVA)。根据实验室结果，鉴定出4种致病真菌，即尖孢镰刀菌(Fusarium oxysporum f. sp. Phaseoli)、solani Rhizoctonia (R. soni)、rolfsii Sclerotium (S. rolfsii)和phaseolina Macrophomina (M. phaseolina)、一种线虫和茎蛆。综合方差分析显示(p <0.0001)。差异也非常显著(p <0.0001)，除出苗率、发病率和严重程度外，其他处理间差异均极显著(p <0.001)。从试验中筛选出7个具有抗性的普通品种(Kufanzik、Roba、Hirna、SeR-125、Cranscope、Tinike、awash1)和2个易感品种(Choire和Argane)。
{"title":"Evaluation of common bean genotypes against root rot complex pathogens in West Hararghe, Eastern Ethiopia","authors":"Usmael Abdela, Elias Meseret","doi":"10.17352/acp.000029","DOIUrl":"https://doi.org/10.17352/acp.000029","url":null,"abstract":"Common bean root rot caused by different fungal pathogens is an important disease affecting common bean (Phaseolus vulgaris L.) production and productivity. In Ethiopia, this disease has become one of the most destructive biotic constraints to common bean production. Information on common bean root rot disease management is lacking for the Ethiopian common bean production system in general and West Hararghe in particular. Therefore, in the 2018 main cropping season, a field experiment was performed on 19 common bean varieties in two research fields, the ‘Bareda and Oda Baso’ farmers’ training centers. The experiment was designed to evaluate the level of resistance of a common bean genotype against root rot complex pathogens under natural conditions using a Randomized Complete Block Design (RCBD) design with three replications. The levels of resistance were evaluated based on seedling emergence, disease incidence, and severity in parallel with grain yield data and subjected to analysis of variance (ANOVA). Based on the laboratory results, four pathogenic fungi, namely, Fusarium oxysporum f. sp. Phaseoli (F. o. f. sp. Phaseoli), Rhizoctonia solani (R. soni), Sclerotium rolfsii (S. rolfsii), and Macrophomina phaseolina (M. phaseolina), a Nematode sp., and stem maggot insect were identified. The combined analysis of variance showed very highly significant variation at (p < 0.0001) across the location. There was also very highly significant variation (p < 0.0001) among treatments except for seedling emergence, incidence, and severity, which were highly significant (p < 0.001). From the experiment, seven common bean varieties (Kufanzik, Roba, Hirna, SeR-125, Cranscope, Tinike, Awash-1) that showed resistance characteristics and two susceptible varieties (Choire and Argane) were selected and recommended.","PeriodicalId":91654,"journal":{"name":"Annals of clinical cytology and pathology","volume":"2014 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136010366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Cytological screening with Human Papillomavirus (HPV) triage for equivocal results has been the routine screening procedure for cervical cancer for years worldwide. The dual-marker stain p16/Ki67 (CINtec PLUS) has been shown to offer high sensitivity and specificity in the triage of women at risk of developing HPV-related precancerous lesions. We evaluated the utility of CINtec PLUS in women with normal cytology and a positive HPV test, to see if this test can be used as a prognostic biomarker. Methods: Women of 18 years or older were assembled between January 2018 and December 2022 at two different study sites. These were cytology negative for intra-epithelial Neoplasia (NILM) and a positive HPV test. The prognostic value of the CINtec PLUS test for NILM samples and the confounding effect of HPV subtype, age, university, and follow-up stage were evaluated. Results: CINtec PLUS was positive in 63 out of 312 (20%) NILM/ HPV-positive cases. The Positive Predictive Value (PPV) of CINtec PLUS was 92% for high-grade dysplastic lesion (HSIL), 64% for NILM, and 31% for low-grade dysplasia. The negative predictive value was 83% for HSIL, 64% for NILM, and 83% for low-grade dysplasia. Conclusion: Adding CINtec PLUS with NILM cytology and HPV-positive test can be an important prognostic tool to identify women at risk for a high-grade dysplastic cervical lesion. Importantly, the test can also be used in primary HPV screening programs. However, women with low-grade dysplasia remain at risk for over- and under-treatment.
{"title":"Combined HPV and CINtec PLUS testing for triaging cervical cancer screening in a Belgian cohort","authors":"Cras Louise, Brock Stefanie, Barbé Kurt, Locy Hanne, Broeckx Glenn, S. Shaira","doi":"10.17352/acp.000028","DOIUrl":"https://doi.org/10.17352/acp.000028","url":null,"abstract":"Background: Cytological screening with Human Papillomavirus (HPV) triage for equivocal results has been the routine screening procedure for cervical cancer for years worldwide. The dual-marker stain p16/Ki67 (CINtec PLUS) has been shown to offer high sensitivity and specificity in the triage of women at risk of developing HPV-related precancerous lesions. We evaluated the utility of CINtec PLUS in women with normal cytology and a positive HPV test, to see if this test can be used as a prognostic biomarker. Methods: Women of 18 years or older were assembled between January 2018 and December 2022 at two different study sites. These were cytology negative for intra-epithelial Neoplasia (NILM) and a positive HPV test. The prognostic value of the CINtec PLUS test for NILM samples and the confounding effect of HPV subtype, age, university, and follow-up stage were evaluated. Results: CINtec PLUS was positive in 63 out of 312 (20%) NILM/ HPV-positive cases. The Positive Predictive Value (PPV) of CINtec PLUS was 92% for high-grade dysplastic lesion (HSIL), 64% for NILM, and 31% for low-grade dysplasia. The negative predictive value was 83% for HSIL, 64% for NILM, and 83% for low-grade dysplasia. Conclusion: Adding CINtec PLUS with NILM cytology and HPV-positive test can be an important prognostic tool to identify women at risk for a high-grade dysplastic cervical lesion. Importantly, the test can also be used in primary HPV screening programs. However, women with low-grade dysplasia remain at risk for over- and under-treatment. ","PeriodicalId":91654,"journal":{"name":"Annals of clinical cytology and pathology","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88430204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Colloid carcinoma pancreas is an infiltrative ductal epithelial neoplasm of the pancreas characteristically denominating a preponderant (> 80%) component of enlarged pools of extracellular stromal mucin pervaded with suspended neoplastic cells. Colloid carcinoma pancreas is a microsatellite stable tumefaction and exhibits KRAS genetic mutation confined to codon 12. Tumefaction is posited to arise from the inverse polarization of cells with stromal mucin glycoproteins facing the intrinsic cellular surface. Cogent clinical symptoms such as abdominal or epigastric pain, pancreatitis, diarrhoea, hyperbilirubinemia or loss of weight are discerned. Tumefaction emerges as an enlarged, well-demarcated lesion with a mean diameter of 5 centimetres and a solid, firm, gelatinous cut surface. Neoplasm is predominantly comprised of enlarged, extracellular accumulates of stromal mucin with minimal carcinoma cells suspended within extra-cellular mucin pools. Cuboidal or columnar epithelial cells configure cribriform or stellate cellular clusters or miniature tubules and strips of columnar cells along with signet ring cells.
{"title":"Gum, sap and canker-colloid carcinoma -pancreas","authors":"B. Anubha","doi":"10.17352/acp.000027","DOIUrl":"https://doi.org/10.17352/acp.000027","url":null,"abstract":"Colloid carcinoma pancreas is an infiltrative ductal epithelial neoplasm of the pancreas characteristically denominating a preponderant (> 80%) component of enlarged pools of extracellular stromal mucin pervaded with suspended neoplastic cells. Colloid carcinoma pancreas is a microsatellite stable tumefaction and exhibits KRAS genetic mutation confined to codon 12. Tumefaction is posited to arise from the inverse polarization of cells with stromal mucin glycoproteins facing the intrinsic cellular surface. Cogent clinical symptoms such as abdominal or epigastric pain, pancreatitis, diarrhoea, hyperbilirubinemia or loss of weight are discerned. Tumefaction emerges as an enlarged, well-demarcated lesion with a mean diameter of 5 centimetres and a solid, firm, gelatinous cut surface. Neoplasm is predominantly comprised of enlarged, extracellular accumulates of stromal mucin with minimal carcinoma cells suspended within extra-cellular mucin pools. Cuboidal or columnar epithelial cells configure cribriform or stellate cellular clusters or miniature tubules and strips of columnar cells along with signet ring cells.","PeriodicalId":91654,"journal":{"name":"Annals of clinical cytology and pathology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73686889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We designed and developed a management guide wheel for cervical cancer to facilitate a standard approach and improve the quality of care in managing patients diagnosed with different stages of cervical cancer. Each step of the wheel considers the patient’s current medical condition, FIGO stage, and the possible treatment modalities for cervical cancer in her case. We reviewed existing international guidelines on the management of cervical cancer and compared their respective recommendations. This guide wheel is based on recommendations by the following organizations: the National Comprehensive Cancer Network (NCCN), the Society of Gynecologic Oncology (SGO), the European Society of Gynaecological Oncology (ESGO), the European Society for Medical Oncology (ESMO), the American Society of Clinical Oncology (ASCO), the British Gynaecological Cancer Society (BGCS) and the International Federation of Gynecology and Obstetrics (FIGO).
{"title":"Development of a management guide wheel for cervical cancer","authors":"Ismail Mahmoud Samy, S. Mahmoud, Ismail Julia, AlKhalifa Muneera, Ismael Rehab, Fathi Reham, Ani Wassan Al, Hajri Shaikha Al, Elhassan Nusiba, AlKawari Mooza, A. Aysha, Raza Gulmeen, Fida Mariam, Zeineldine Alaa, Torsten Uwe","doi":"10.17352/acp.000025","DOIUrl":"https://doi.org/10.17352/acp.000025","url":null,"abstract":"We designed and developed a management guide wheel for cervical cancer to facilitate a standard approach and improve the quality of care in managing patients diagnosed with different stages of cervical cancer. Each step of the wheel considers the patient’s current medical condition, FIGO stage, and the possible treatment modalities for cervical cancer in her case. We reviewed existing international guidelines on the management of cervical cancer and compared their respective recommendations. This guide wheel is based on recommendations by the following organizations: the National Comprehensive Cancer Network (NCCN), the Society of Gynecologic Oncology (SGO), the European Society of Gynaecological Oncology (ESGO), the European Society for Medical Oncology (ESMO), the American Society of Clinical Oncology (ASCO), the British Gynaecological Cancer Society (BGCS) and the International Federation of Gynecology and Obstetrics (FIGO).","PeriodicalId":91654,"journal":{"name":"Annals of clinical cytology and pathology","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79932613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ismail’s Cancer Vulva Management Guidewheel is designed to enable physicians to refer to a quick and comprehensive reference to recommendations on the management of vulvar cancer at various stages. The wheel consists of patient characteristics, including the FIGO stage and the possible treatment modalities available along with alternatives. We compared guidelines on the management of vulvar cancer by international health organizations including the National Comprehensive Cancer Network (NCCN), the Society of Gynecologic Oncology (SGO), the European Society of Gynaecological Oncology (ESGO), the European Society of Urogenital Radiology (ESUR), the British Gynaecological Cancer Society (BGCS), the Royal College of Obstetricians and Gynaecologists (RCOG), the Japanese Society of Gynecologic Oncology (JSGO), the Society of Obstetricians and Gynaecologists of Canada (SOGC) and the International Federation of Gynecology and Obstetrics (FIGO). Their respective recommendations have been compared and carefully integrated on the guidewheel.
{"title":"Development of a management guidewheel for vulvar cancer","authors":"Ismail Mahmoud Samy, I. Fouad, Ismail Julia, AlKhalifa Muneera, Ismael Rehab, Fathi Reham, Ani Wassan Al, Hajri Shaikha Al, Fida Mariam, Zeineldine Alaa, Torsten Uwe","doi":"10.17352/acp.000024","DOIUrl":"https://doi.org/10.17352/acp.000024","url":null,"abstract":"Ismail’s Cancer Vulva Management Guidewheel is designed to enable physicians to refer to a quick and comprehensive reference to recommendations on the management of vulvar cancer at various stages. The wheel consists of patient characteristics, including the FIGO stage and the possible treatment modalities available along with alternatives. We compared guidelines on the management of vulvar cancer by international health organizations including the National Comprehensive Cancer Network (NCCN), the Society of Gynecologic Oncology (SGO), the European Society of Gynaecological Oncology (ESGO), the European Society of Urogenital Radiology (ESUR), the British Gynaecological Cancer Society (BGCS), the Royal College of Obstetricians and Gynaecologists (RCOG), the Japanese Society of Gynecologic Oncology (JSGO), the Society of Obstetricians and Gynaecologists of Canada (SOGC) and the International Federation of Gynecology and Obstetrics (FIGO). Their respective recommendations have been compared and carefully integrated on the guidewheel.","PeriodicalId":91654,"journal":{"name":"Annals of clinical cytology and pathology","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80390932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lung cancer is the most common cause of cancer-related death worldwide and unfortunately up to 80% of patients amongst newly diagnosed are inoperable therefore the cytological sample is often the only material available for diagnosis and assessment of molecular characteristics driving the treatment.
{"title":"Evaluation of angiogenesis in cervical cancer using CD34 as a biomarker and its correlation with pathoanatomical features","authors":"M. Aijaz, K. Alam, V. Maheshwari","doi":"10.17352/acp.000023","DOIUrl":"https://doi.org/10.17352/acp.000023","url":null,"abstract":"Lung cancer is the most common cause of cancer-related death worldwide and unfortunately up to 80% of patients amongst newly diagnosed are inoperable therefore the cytological sample is often the only material available for diagnosis and assessment of molecular characteristics driving the treatment.","PeriodicalId":91654,"journal":{"name":"Annals of clinical cytology and pathology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85692321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A dye or stain is capable of penetrating the living cells or tissues and not inducing immediate degenerative changes. Vital stain means a stain that can be applied on a living cells without killing them.
{"title":"Vital Staining- Pivotal Role in the Field of Pathology","authors":"K. Nitya, Vikram S. Amberkar, B. Nadar","doi":"10.17352/acp.000017","DOIUrl":"https://doi.org/10.17352/acp.000017","url":null,"abstract":"A dye or stain is capable of penetrating the living cells or tissues and not inducing immediate degenerative changes. Vital stain means a stain that can be applied on a living cells without killing them.","PeriodicalId":91654,"journal":{"name":"Annals of clinical cytology and pathology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90393006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sex-determining region y (SRY)-Box2 (SOX2) & L1-cell adhesion molecule (L1CAM) expressions in endometrioid carcinoma of the uterus; An Immunohistochemical Study","authors":"Salem Amir M","doi":"10.17352/acp.000010","DOIUrl":"https://doi.org/10.17352/acp.000010","url":null,"abstract":"","PeriodicalId":91654,"journal":{"name":"Annals of clinical cytology and pathology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90439813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarmed H Kathem, Wissam A AbouAlaiwi, Xiaolin Zi, Surya M Nauli
Bilateral renal cyst formation is the main feature of autosomal dominant polycystic kidney disease (ADPKD). We and other laboratories have previously shown that cyst-lining epithelia of kidneys from ADPKD patients are characterized by polyploidy. In this report, we show that endothelia from the renal capillary beds of two ADPKD patients are also polyploidy. Spectral karyotyping study further confirms our flow cytometry analyses. We suggest that polyploidy may be used as a potential cellular marker in ADPKD.
{"title":"Capillary endothelia from two ADPKD patients are polyploidy.","authors":"Sarmed H Kathem, Wissam A AbouAlaiwi, Xiaolin Zi, Surya M Nauli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bilateral renal cyst formation is the main feature of autosomal dominant polycystic kidney disease (ADPKD). We and other laboratories have previously shown that cyst-lining epithelia of kidneys from ADPKD patients are characterized by polyploidy. In this report, we show that endothelia from the renal capillary beds of two ADPKD patients are also polyploidy. Spectral karyotyping study further confirms our flow cytometry analyses. We suggest that polyploidy may be used as a potential cellular marker in ADPKD.</p>","PeriodicalId":91654,"journal":{"name":"Annals of clinical cytology and pathology","volume":"2 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436797/pdf/nihms-838965.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35015563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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