Pub Date : 2018-09-01Epub Date: 2018-09-30DOI: 10.31665/JFB.2018.3154
Bayissi Bading-Taika, Tunde Akinyeke, Armando Alcazar Magana, Jaewoo Choi, Michael Ouanesisouk, Eileen Ruth Samson Torres, Lisa A Lione, Claudia S Maier, Gerd Bobe, Jacob Raber, Cristobal L Miranda, Jan F Stevens
Preparations of the root bark of Tabernanthe iboga have long been used in Central and West African traditional medicine to combat fatigue, as a neuro-stimulant in rituals, and for treatment of diabetes. The principal alkaloid of T. iboga, ibogaine, has attracted attention in many countries around the world for providing relief for opioid craving in drug addicts. Using a plant metabolomics approach, we detected five phenolic compounds, including 3-O-caffeoylquinic acid, and 30 alkaloids, seven of which were previously reported from T. iboga root bark. Following a report that iboga extracts contain insulinotropic agents, we aimed to determine the potential alleviating effects of the water extract of iboga root bark on high-fat diet (HFD)-induced hyperglycemia as well as its effects on cognitive function in male C57BL/6J mice. Feeding a HFD to mice for 10 weeks produced manifestations of metabolic syndrome such as increased body weight and increased plasma levels of glucose, triacylglycerols, total cholesterol, LDL-cholesterol, insulin, leptin, and pro-inflammatory mediators (IL-6, MCP-1, ICAM-1), as compared to mice fed a low-fat diet (LFD). Supplementation of HFD with iboga extract at ibogaine doses of 0.83 (low) and 2.07 (high) mg/kg/day did not improve these HFD-induced metabolic effects except for a reduction of plasma MCP-1 in the low dose group, indicative of an anti-inflammatory effect. When the HFD mice were tested in the water maze, the high-dose iboga extract caused hippocampus-dependent impairments in spatial learning and memory, as compared to mice receiving only a HFD.
长期以来，Tabernanthe iboga的根皮制剂一直被用于中非和西非的传统医学中，以对抗疲劳，在仪式中作为神经兴奋剂，并用于治疗糖尿病。伊博格的主要生物碱伊博格碱因能缓解吸毒者对阿片类药物的渴望而引起了世界许多国家的关注。利用植物代谢组学方法，我们检测到5种酚类化合物，包括3- o -咖啡基奎宁酸和30种生物碱，其中7种是以前从T. iboga根皮中报道的。在有报道称伊博加提取物含有促胰岛素剂之后，我们旨在确定伊博加根树皮水提取物对高脂肪饮食(HFD)诱导的高血糖的潜在缓解作用及其对雄性C57BL/6J小鼠认知功能的影响。与饲喂低脂饮食(LFD)的小鼠相比，饲喂高脂饮食10周的小鼠产生代谢综合征的表现，如体重增加，血浆葡萄糖、甘油三酯、总胆固醇、低密度脂蛋白胆固醇、胰岛素、瘦素和促炎介质(IL-6、MCP-1、ICAM-1)水平升高。在伊博格碱剂量为0.83(低)和2.07(高)mg/kg/天的伊博格碱组中，除了低剂量组血浆MCP-1降低外，HFD与伊博格碱提取物的补充并没有改善这些HFD诱导的代谢效应，表明其具有抗炎作用。当HFD小鼠在水迷宫中进行测试时，与仅接受HFD的小鼠相比，高剂量的iboga提取物引起了海马体依赖性的空间学习和记忆障碍。
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