Marine and freshwater harmful algae : proceedings of the 16th International Conference on Harmful Algae, 27th-31st October 2014, Wellington, New Zealand. International Conference on Harmful Algae (16th : 2014 : Wellington, N.Z.), author最新文献

英文中文

Translating the message: Karlodinium veneficum possesses an expanded toolkit of protein translation initiation factors. 翻译信息：Karlodinium veneficum 拥有一个扩展的蛋白质翻译启动因子工具包。

Marine and freshwater harmful algae : proceedings of the 16th International Conference on Harmful Algae, 27th-31st October 2014, Wellington, New Zealand. International Conference on Harmful Algae (16th : 2014 : Wellington, N.Z.), author

Pub Date : 2015-01-01

Grant D Jones, Ernest P Williams, Tsvetan R Bachvaroff, Allen R Place, Rosemary Jagus

Dinoflagellates are unusual eukaryotes with large genomes and a reduced role for transcriptional regulation compared to other eukaryotes. The mRNA in dinoflagellates is trans-spliced with a 5'-spliced-leader sequence, yielding a 22-nucleotide 5'-sequence with a methylated nucleotide cap. Since the control of gene expression is primarily post-transcriptional, this study focuses on mRNA recruitment as a means for regulating gene expression and specifically on the diversity of eIF4E family members. Three novel clades related to the cap binding initiation factor eIF4E have been recognized in alveolates that are distinct from the three metazoan classes of eIF4E. We have analyzed the characteristics of five of the fourteen eIF4E family members from Karlodinium veneficum, four from clade 1 and one from clade 2. Members of each clade all bear the distinctive features of a cap-binding protein. We examined their ability to interact with the cap analogue, m⁷GTP using an in vitro bead-binding assay. We show that recombinant eIF4E-1 family members are able to bind the cap analogue m⁷GTP, but eIF4E-2b binds poorly. Overall, the eIF4E-1 family members may be serving as general cap-binding translation initiation factors, while the eIF4E-2 (and perhaps eIF4E-3) family members may serve a regulatory role in gene expression.

甲藻是不常见的真核生物，基因组庞大，与其他真核生物相比，转录调控的作用较小。甲藻的 mRNA 通过 5'-spliced-leader 序列进行反式剪接，产生带有甲基化核苷酸帽的 22 个核苷酸的 5'-sequence 。由于基因表达的调控主要是转录后调控，因此本研究侧重于 mRNA 招募作为调控基因表达的一种手段，特别是 eIF4E 家族成员的多样性。在肺泡动物中发现了三个与帽子结合起始因子 eIF4E 相关的新支系，它们有别于三种元虫类的 eIF4E。我们分析了 Karlodinium veneficum 的 14 个 eIF4E 家族成员中的 5 个成员的特征，其中 4 个来自支系 1，1 个来自支系 2。每个支系的成员都具有帽子结合蛋白的显著特征。我们利用体外珠子结合试验检验了它们与帽子类似物 m7GTP 相互作用的能力。我们发现，重组的 eIF4E-1 家族成员能够与帽子类似物 m7GTP 结合，但 eIF4E-2b 的结合能力较差。总的来说，eIF4E-1 家族成员可能是一般的帽结合翻译起始因子，而 eIF4E-2（也许还有 eIF4E-3）家族成员可能在基因表达中起调控作用。

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