Carolyn Y Dadabay, Paula Barrientos Spaulding, Erick Valenzuela, Matthew Turner, Kaitlyn E Eckert, Riitta Julkunen-Tiitto, Nedra Noblit, Donald H Mansfield
Basin big sagebrush (Artemisia tridentata Nutt. ssp. tridentata (Asteraceae)), is a widespread North American shrub which produces a variety of polyphenolic compounds. Although sagebrush has been used as a traditional remedy by natives and settlers to the region, the polyphenols in Artemisia tridentata ssp. tridentata have not been highly investigated for their bioactive properties. To determine whether these polyphenols affect the intracellular redox state, we measured their ability to neutralize radicals in vitro and in a human liver carcinoma cell line (HepG2), and their effects on intracellular glutathione levels. Extracts from Artemisia tridentata ssp. tridentata decreased the oxidation of 2'7'-dichlorofluorescin in vitro and in cultured cells. Cells treated with polyphenolic extracts showed increased levels of glutathione in a time and dose-dependent manner. Approximately 48 polyphenolic compounds were distinguishable in extracts, by HPLC/UV absorbance detection. Mass spectroscopy was used to identify thirteen compounds as aesculin, aesculetin, apigenin, apigenin-7-O-glucoside, axillarin, casticin, chlorogenic acid, isoscopoletin, kaempferol, luteolin, methyl-axillarin, quercetin, and scopoletin. These results indicate that polyphenols produced in Artemisia tridentata ssp. tridentata affect the redox state of living cells by multiple mechanisms.
盆地大山艾属(蒿属)。ssp。tridentata (Asteraceae))，是一种广泛分布的北美灌木，产生多种多酚化合物。虽然山艾草一直被当地人和该地区的定居者用作传统的药物，但三叶蒿中的多酚。三叉戟属植物的生物活性尚未得到充分的研究。为了确定这些多酚是否影响细胞内氧化还原状态，我们在体外和人肝癌细胞系(HepG2)中测量了它们中和自由基的能力，以及它们对细胞内谷胱甘肽水平的影响。三叶蒿提取物。在体外和培养细胞中，三叉戟能降低2'7'-二氯荧光素的氧化。用多酚提取物处理的细胞显示谷胱甘肽水平以时间和剂量依赖的方式增加。通过HPLC/UV吸光度检测，提取液中可分离出约48种多酚类化合物。用质谱法鉴定了13种化合物，分别为七叶皂苷、七叶皂苷、芹菜素、芹菜素-7- o -葡萄糖苷、茜草素、蓖麻素、绿原酸、异东莨菪素、山奈酚、木犀草素、甲基茜草素、槲皮素和东莨菪素。这些结果表明，三叶蒿中多酚类物质的产生具有一定的化学活性。三叉戟通过多种机制影响活细胞的氧化还原状态。
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