C Sampath, E P Harris, V Berthaud, M A Tabatabai, D M Wilus, M A Crayton, K Moss, J Webster-Cyriaque, J H Southerland, J R Koethe, P R Gangula
Introduction: Periodontal Disease (PD), a chronic inflammatory disease, is highly prevalent among Persons Living With HIV (PLWH) and is characterized by microbial symbiosis and oxidative stress. Our hypothesis stipulates that periodontal therapy attenuates systemic inflammatory and bacterial burden while improving periodontal status in PLWH.
Methods: Sixteen African Americans (AA) with suppressed HIV viremia on long-term Antiretroviral Therapy (ART) were recruited to this study. Participants were placed into two groups, based on their dental care status: group 1 (In-Care, IC) and group 2 (Out of Care, OC). Periodontal health was investigated at baseline, 3 months, 6 months, and 12 months. Cytokine/chemokines, microbial phyla, and Asymmetric Dimethylarginine (ADMA, a marker for endothelial cell dysfunction) levels were assessed in the serum. Statistical comparisons between groups and at different visits were performed using multiple comparison tests.
Results: Across longitudinal visits, periodontal treatment significantly reduced the levels of several cytokines and chemokines. At baseline, the out of care group had significantly higher blood levels of ADMA and actinobacteria than the IC group. Periodontal treatment significantly altered the abundance of circulating genomic bacterial DNA for various phyla in out of care group.
Conclusions: Periodontal treatment interventions effectively attenuated circulating pro-inflammatory cytokines and altered microbial translocation, both critical drivers of systemic inflammation in PLWH.
导言：牙周病（PD）是一种慢性炎症性疾病，在艾滋病病毒感染者（PLWH）中发病率很高，其特点是微生物共生和氧化应激。我们的假设是，牙周治疗可减轻全身炎症和细菌负担，同时改善艾滋病病毒感染者的牙周状况：本研究招募了 16 名长期接受抗逆转录病毒疗法（ART）的非裔美国人（AA）。根据参与者的牙科保健状况将其分为两组：第一组（护理中，IC）和第二组（护理外，OC）。分别在基线、3个月、6个月和12个月时对牙周健康状况进行调查。对血清中的细胞因子/趋化因子、微生物系统和不对称二甲基精氨酸（ADMA，内皮细胞功能障碍的标志物）水平进行了评估。采用多重比较检验对不同组间和不同访问进行统计比较：结果：在纵向观察中，牙周治疗显著降低了多种细胞因子和趋化因子的水平。基线时，非治疗组血液中的 ADMA 和放线菌水平明显高于 IC 组。牙周治疗明显改变了失治组各系统循环基因组细菌DNA的丰度：结论：牙周治疗干预措施能有效减少循环中的促炎细胞因子并改变微生物的转运，这两种因素都是导致 PLWH 全身炎症的关键因素。
{"title":"Periodontal Treatment Reduces Circulating Pro-Inflammatory Cytokine and Chemokine Levels in African American HIV+ Individuals with Virological Suppression.","authors":"C Sampath, E P Harris, V Berthaud, M A Tabatabai, D M Wilus, M A Crayton, K Moss, J Webster-Cyriaque, J H Southerland, J R Koethe, P R Gangula","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Introduction: </strong>Periodontal Disease (PD), a chronic inflammatory disease, is highly prevalent among Persons Living With HIV (PLWH) and is characterized by microbial symbiosis and oxidative stress. Our hypothesis stipulates that periodontal therapy attenuates systemic inflammatory and bacterial burden while improving periodontal status in PLWH.</p><p><strong>Methods: </strong>Sixteen African Americans (AA) with suppressed HIV viremia on long-term Antiretroviral Therapy (ART) were recruited to this study. Participants were placed into two groups, based on their dental care status: group 1 (In-Care, IC) and group 2 (Out of Care, OC). Periodontal health was investigated at baseline, 3 months, 6 months, and 12 months. Cytokine/chemokines, microbial phyla, and Asymmetric Dimethylarginine (ADMA, a marker for endothelial cell dysfunction) levels were assessed in the serum. Statistical comparisons between groups and at different visits were performed using multiple comparison tests.</p><p><strong>Results: </strong>Across longitudinal visits, periodontal treatment significantly reduced the levels of several cytokines and chemokines. At baseline, the out of care group had significantly higher blood levels of ADMA and actinobacteria than the IC group. Periodontal treatment significantly altered the abundance of circulating genomic bacterial DNA for various phyla in out of care group.</p><p><strong>Conclusions: </strong>Periodontal treatment interventions effectively attenuated circulating pro-inflammatory cytokines and altered microbial translocation, both critical drivers of systemic inflammation in PLWH.</p>","PeriodicalId":93776,"journal":{"name":"Journal of dental applications","volume":"8 1","pages":"477-487"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583701/pdf/nihms-1833397.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40653474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-29DOI: 10.26420/jdentapp.2021.1114
S. I., Sharon E, Hendler A, B. G, Beytha N
Purpose: Ultrasonic scaler is frequently used for temporary cement remnants removal from abutment teeth. A major concern is the alteration of the structural integrity of the abutment finishing line by the ultrasonic scaler. The current study evaluates the influence of ultrasonic scaling on the integrity of the finish line. The effect of finish line design (chamfer or feather edge), and its location (dentin or enamel) was also evaluated. Methods: Intact 22 human extracted molars were divided into 2 groups: finish line was prepared on enamel (n=11) and dentin (n=11), and further subdivided to finish line configuration groups (chamfer or knife-edge). Preparation of finish line was preformed following a standard repetitive procedure. 3D scans of the same tooth before and after ultrasonic instrumentation were performed. Pre and post scaling images were superimposed. The finish line alterations were evaluated and measured. The significance of the differences was evaluated using Students’ t-test. Results: The average alteration at the finish line area following ultrasonic scaling was 71.5 ± 24.6 μm. Higher alterations were found for enamel and for chamfer finish lines compared to dentin and knife edge, 134.4 ± 61.4 μm and 30.3 ±11.7 μm respectively. The highest change (228.6μm) was found for chamfer finish line located on enamel. The differences between groups were statistically significant (p<0.05). Conclusion: Ultrasonic scaling alters the topography of the finish line area of abutment teeth and thus may compromise marginal fit and integrity of the final restoration.
{"title":"The Effect of Ultrasonic Instrumentation on Finish Line Integrity","authors":"S. I., Sharon E, Hendler A, B. G, Beytha N","doi":"10.26420/jdentapp.2021.1114","DOIUrl":"https://doi.org/10.26420/jdentapp.2021.1114","url":null,"abstract":"Purpose: Ultrasonic scaler is frequently used for temporary cement remnants removal from abutment teeth. A major concern is the alteration of the structural integrity of the abutment finishing line by the ultrasonic scaler. The current study evaluates the influence of ultrasonic scaling on the integrity of the finish line. The effect of finish line design (chamfer or feather edge), and its location (dentin or enamel) was also evaluated. Methods: Intact 22 human extracted molars were divided into 2 groups: finish line was prepared on enamel (n=11) and dentin (n=11), and further subdivided to finish line configuration groups (chamfer or knife-edge). Preparation of finish line was preformed following a standard repetitive procedure. 3D scans of the same tooth before and after ultrasonic instrumentation were performed. Pre and post scaling images were superimposed. The finish line alterations were evaluated and measured. The significance of the differences was evaluated using Students’ t-test. Results: The average alteration at the finish line area following ultrasonic scaling was 71.5 ± 24.6 μm. Higher alterations were found for enamel and for chamfer finish lines compared to dentin and knife edge, 134.4 ± 61.4 μm and 30.3 ±11.7 μm respectively. The highest change (228.6μm) was found for chamfer finish line located on enamel. The differences between groups were statistically significant (p<0.05). Conclusion: Ultrasonic scaling alters the topography of the finish line area of abutment teeth and thus may compromise marginal fit and integrity of the final restoration.","PeriodicalId":93776,"journal":{"name":"Journal of dental applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46028922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-13DOI: 10.26420/jdentapp.2021.1113
J. Mezzadri, Faculty Herrero Álvaro Andrade Curitiba Paraná Brazil Graduation Program, K. Silva, Moraes Gf, Manfron Apt
To promote an effective and predictable treatment planning in cases of gingival smile correction, a periodontal surgical guided from virtual planning could be used. The guide was designed using planning software, based on the patient’s facial aesthetic analysis, photos and intraoral scanning. As a result, the surgical procedure by digital planning provided a more predictable, personalized and safe treatment and outcome to the patient. The present case report describes a gingival smile correction, using digital planning combined with high-power diode laser for periodontal surgery. After 3 months of preservation, there was less exposure of the gingiva in the smile and a high level of patient aesthetic satisfaction. Considering the importance of the correct treatment planning, it may be concluded that use of surgical guided made from a digital planning provided a predictable, personalized and safe treatment to the patient.
{"title":"Guided Periodontal Surgery: Digital Workflow for Correction of a Gingival Smile","authors":"J. Mezzadri, Faculty Herrero Álvaro Andrade Curitiba Paraná Brazil Graduation Program, K. Silva, Moraes Gf, Manfron Apt","doi":"10.26420/jdentapp.2021.1113","DOIUrl":"https://doi.org/10.26420/jdentapp.2021.1113","url":null,"abstract":"To promote an effective and predictable treatment planning in cases of gingival smile correction, a periodontal surgical guided from virtual planning could be used. The guide was designed using planning software, based on the patient’s facial aesthetic analysis, photos and intraoral scanning. As a result, the surgical procedure by digital planning provided a more predictable, personalized and safe treatment and outcome to the patient. The present case report describes a gingival smile correction, using digital planning combined with high-power diode laser for periodontal surgery. After 3 months of preservation, there was less exposure of the gingiva in the smile and a high level of patient aesthetic satisfaction. Considering the importance of the correct treatment planning, it may be concluded that use of surgical guided made from a digital planning provided a predictable, personalized and safe treatment to the patient.","PeriodicalId":93776,"journal":{"name":"Journal of dental applications","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41740076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-26DOI: 10.26420/jdentapp.2021.1112
Li Q
Dental Pulp Stem Cells (DPSCs) are adult stem cells found in dental pulp tissue, and possess the capacity for self-renewal and the potential for multipotent differentiation. DPSCs depend on regulating in vitro microenvironment, and can readily differentiate into osteoblasts, odontoblasts, neurocytes, adipocytes, chondrocytes, myocytes, fibrocytes and many others. In addition, DPSCs play a crucial role in tooth regeneration, bone and nerve repair in current studies of regenerative medicine and tissue engineering. Among them, the influences of stem cell microenvironment or niche on the biological activity of DPSCs are critical and hamper its progress. Herein, we review the influence of culture condition, tissue source, growth factor requirements, and cellular organizational scaffolds and how these features influence the biological characteristics and translational research of DPSCs.
{"title":"Importance of In Vitro Microenvironment on Differentiation and Translational Applications of Dental Pulp Stem Cells","authors":"Li Q","doi":"10.26420/jdentapp.2021.1112","DOIUrl":"https://doi.org/10.26420/jdentapp.2021.1112","url":null,"abstract":"Dental Pulp Stem Cells (DPSCs) are adult stem cells found in dental pulp tissue, and possess the capacity for self-renewal and the potential for multipotent differentiation. DPSCs depend on regulating in vitro microenvironment, and can readily differentiate into osteoblasts, odontoblasts, neurocytes, adipocytes, chondrocytes, myocytes, fibrocytes and many others. In addition, DPSCs play a crucial role in tooth regeneration, bone and nerve repair in current studies of regenerative medicine and tissue engineering. Among them, the influences of stem cell microenvironment or niche on the biological activity of DPSCs are critical and hamper its progress. Herein, we review the influence of culture condition, tissue source, growth factor requirements, and cellular organizational scaffolds and how these features influence the biological characteristics and translational research of DPSCs.","PeriodicalId":93776,"journal":{"name":"Journal of dental applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45284239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-29DOI: 10.26420/jdentapp.2021.1111
P WiedelA, H. Svensson, K. Hellén-Halme, H. Ghaffari, M. Becker, Oral
Background: The aim of this study was to investigate whether a complementary Cone-Beam Computed Tomography (CBCT) in patients with Cleft Lip and Palate (CLP) after alveolar bone-grafting to clefts gave substantial additional information, and particularly whether such new information had any implications for the further care of the patients. Methods: Seventeen children, with complete CLP, 10 unilateral and seven bilateral clefts, in all 24 clefts, were evaluated six months after secondary alveolar bone-grafting with two-dimensional intra-oral radiographs complemented with CBCT. The mean age at bone-grafting was 8.8 years. Three different examiners evaluated the radiographic documentation. Results: The mean pre-operative cleft width was 5.8mm. In 15 of the 24 clefts the same interpretation was made on both two-dimensional radiographs and CBCT. In the remaining nine clefts, CBCT added important information to the treatment decision. Conclusions: For the evaluation six months post-operatively of the success of alveolar bone-grafting to clefts, the two-dimensional radiograph should be complemented with CBCT unless the two-dimensional radiograph without doubt reveals open residual cleft and clinical findings indicate graft failure.
{"title":"Two-Dimensional Intra-Oral Radiographs Compared to Three-Dimensional CBCT at Six-Month Post-Operative Evaluation of Secondary Bone-Grafting in Patients with Cleft Lip and Palate","authors":"P WiedelA, H. Svensson, K. Hellén-Halme, H. Ghaffari, M. Becker, Oral","doi":"10.26420/jdentapp.2021.1111","DOIUrl":"https://doi.org/10.26420/jdentapp.2021.1111","url":null,"abstract":"Background: The aim of this study was to investigate whether a complementary Cone-Beam Computed Tomography (CBCT) in patients with Cleft Lip and Palate (CLP) after alveolar bone-grafting to clefts gave substantial additional information, and particularly whether such new information had any implications for the further care of the patients. Methods: Seventeen children, with complete CLP, 10 unilateral and seven bilateral clefts, in all 24 clefts, were evaluated six months after secondary alveolar bone-grafting with two-dimensional intra-oral radiographs complemented with CBCT. The mean age at bone-grafting was 8.8 years. Three different examiners evaluated the radiographic documentation. Results: The mean pre-operative cleft width was 5.8mm. In 15 of the 24 clefts the same interpretation was made on both two-dimensional radiographs and CBCT. In the remaining nine clefts, CBCT added important information to the treatment decision. Conclusions: For the evaluation six months post-operatively of the success of alveolar bone-grafting to clefts, the two-dimensional radiograph should be complemented with CBCT unless the two-dimensional radiograph without doubt reveals open residual cleft and clinical findings indicate graft failure.","PeriodicalId":93776,"journal":{"name":"Journal of dental applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45218363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.26420/jdentapp.2021.1110
S. Z, J. X, C. L
This study displays a retrospective cohort analysis in the Reproductive and Genetic Health Center of Peking University First Hospital. Data were collected from 411 women receiving ART. The body composition, sex hormones and biochemical indicators were assessed by Bioelectrical Impedance Analysis (BIA). Subjects were grouped by Body Mass Index (BMI) and Body Fat Percentage (BFP), and differences of body composition among groups were analyzed. Multiple linear regression analysis was used to evaluate the relationship between sex hormones, biochemical indicators and body composition. Compared with the non-overweight group (BMI <24kg/m²), the lean body mass and fat mass of the overweight group (BMI ≥24kg/m²) increased significantly (P <0.001). Notably, 43.7% of non-overweight subjects have increased body fat as distinguished by PBF. The visceral adipose tissue increased by 50.9% in subjects with more fat. Fasting Blood Glucose (FBG), triglyceride, Low Density Lipoprotein Cholesterol (LDL-C) and testosterone were positively correlated with trunk fat, while High Density Lipoprotein Cholesterol (HDL-C) and estradiol were negatively correlated with trunk fat. Moreover, total cholesterol was positively correlated with Bone Mineral Content (BMC). Therefore, this research demonstrated significant differences in body composition between overweight and non-overweight women receiving ART. Combining PBF with BMI, obesity can be identified more accurately. Biochemical metabolism and sex hormones were related to body composition. Therefore, women receiving ART require detailed measurements of body composition, not BMI. Further studies are needed to confirm the relationship between pregnancy outcomes and body composition in women receiving ART treatment.
本研究对北京大学第一医院生殖与遗传健康中心的患者进行回顾性队列分析。数据来自411名接受抗逆转录病毒治疗的妇女。采用生物电阻抗分析法(BIA)测定各组体成分、性激素及生化指标。采用体质量指数(BMI)和体脂率(BFP)进行分组，分析各组体成分的差异。采用多元线性回归分析评价性激素、生化指标与体成分的关系。与非超重组(BMI <24kg/m²)相比，超重组(BMI≥24kg/m²)的瘦体质量和脂肪质量显著增加(P <0.001)。值得注意的是，通过PBF来区分，43.7%的非超重受试者体脂增加。脂肪越多，内脏脂肪组织增加了50.9%。空腹血糖(FBG)、甘油三酯(triglyceride)、低密度脂蛋白胆固醇(Low Density Lipoprotein Cholesterol, LDL-C)、睾酮与躯干脂肪呈显著正相关，高密度脂蛋白胆固醇(High Density Lipoprotein Cholesterol, HDL-C)、雌二醇与躯干脂肪呈显著负相关。此外，总胆固醇与骨矿物质含量(BMC)呈正相关。因此，本研究证明接受抗逆转录病毒治疗的超重和非超重女性在身体组成上存在显著差异。结合PBF和BMI，可以更准确地识别肥胖。生化代谢和性激素与体成分有关。因此，接受抗逆转录病毒治疗的妇女需要详细测量身体成分，而不是身体质量指数。需要进一步的研究来证实接受抗逆转录病毒治疗的妇女的妊娠结局与身体组成之间的关系。
{"title":"The Body Composition of Women Undergoing ART, and Its Relationship with Sex Hormones and Biochemical Indicators","authors":"S. Z, J. X, C. L","doi":"10.26420/jdentapp.2021.1110","DOIUrl":"https://doi.org/10.26420/jdentapp.2021.1110","url":null,"abstract":"This study displays a retrospective cohort analysis in the Reproductive and Genetic Health Center of Peking University First Hospital. Data were collected from 411 women receiving ART. The body composition, sex hormones and biochemical indicators were assessed by Bioelectrical Impedance Analysis (BIA). Subjects were grouped by Body Mass Index (BMI) and Body Fat Percentage (BFP), and differences of body composition among groups were analyzed. Multiple linear regression analysis was used to evaluate the relationship between sex hormones, biochemical indicators and body composition. Compared with the non-overweight group (BMI <24kg/m²), the lean body mass and fat mass of the overweight group (BMI ≥24kg/m²) increased significantly (P <0.001). Notably, 43.7% of non-overweight subjects have increased body fat as distinguished by PBF. The visceral adipose tissue increased by 50.9% in subjects with more fat. Fasting Blood Glucose (FBG), triglyceride, Low Density Lipoprotein Cholesterol (LDL-C) and testosterone were positively correlated with trunk fat, while High Density Lipoprotein Cholesterol (HDL-C) and estradiol were negatively correlated with trunk fat. Moreover, total cholesterol was positively correlated with Bone Mineral Content (BMC). Therefore, this research demonstrated significant differences in body composition between overweight and non-overweight women receiving ART. Combining PBF with BMI, obesity can be identified more accurately. Biochemical metabolism and sex hormones were related to body composition. Therefore, women receiving ART require detailed measurements of body composition, not BMI. Further studies are needed to confirm the relationship between pregnancy outcomes and body composition in women receiving ART treatment.","PeriodicalId":93776,"journal":{"name":"Journal of dental applications","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69278621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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