首页 > 最新文献

Archives of oral biology最新文献

英文 中文
The influence of microorganisms on bone homeostasis in apical periodontitis.
Pub Date : 2024-11-30 DOI: 10.1016/j.archoralbio.2024.106153
Dan Pan, Yu Hao, Yuyan Tao, Bolei Li, Lei Cheng

Objective: This review aims to provide an overview of the role of microorganisms in the onset and progression of periapical diseases, particularly regarding their effects on bone homeostasis.

Design: The search for this narrative review was conducted in PubMed, Web of Science and Google Scholar using relevant keywords, including checking reference lists of journal articles by hand searching.

Results: Microorganisms directly promote osteoclasts through pathways such as nuclear factor-κB (NF-κB) and extracellular regulated protein kinases (ERK), while inhibiting osteoblasts function by interfering with the wingless-related integration site (Wnt)/β-catenin pathway in the periapical area. Moreover, microorganisms indirectly regulate periapical bone homeostasis by inducing programmed cell death and modulating the immune microenvironment through the activation of innate immunity via pattern-recognition receptors (PRRs) and subsequent cascades of responses. Among these microorganisms, Enterococcus faecalis, Porphyromonas gingivalis and Fusobacterium nucleatum play significant roles.

Conclusion: Microorganisms regulate pathways such as NF-ĸB and Wnt/β-catenin, as well as programmed cell death and the immune microenvironment in the periapical area, thereby disrupting bone homeostasis.

{"title":"The influence of microorganisms on bone homeostasis in apical periodontitis.","authors":"Dan Pan, Yu Hao, Yuyan Tao, Bolei Li, Lei Cheng","doi":"10.1016/j.archoralbio.2024.106153","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106153","url":null,"abstract":"<p><strong>Objective: </strong>This review aims to provide an overview of the role of microorganisms in the onset and progression of periapical diseases, particularly regarding their effects on bone homeostasis.</p><p><strong>Design: </strong>The search for this narrative review was conducted in PubMed, Web of Science and Google Scholar using relevant keywords, including checking reference lists of journal articles by hand searching.</p><p><strong>Results: </strong>Microorganisms directly promote osteoclasts through pathways such as nuclear factor-κB (NF-κB) and extracellular regulated protein kinases (ERK), while inhibiting osteoblasts function by interfering with the wingless-related integration site (Wnt)/β-catenin pathway in the periapical area. Moreover, microorganisms indirectly regulate periapical bone homeostasis by inducing programmed cell death and modulating the immune microenvironment through the activation of innate immunity via pattern-recognition receptors (PRRs) and subsequent cascades of responses. Among these microorganisms, Enterococcus faecalis, Porphyromonas gingivalis and Fusobacterium nucleatum play significant roles.</p><p><strong>Conclusion: </strong>Microorganisms regulate pathways such as NF-ĸB and Wnt/β-catenin, as well as programmed cell death and the immune microenvironment in the periapical area, thereby disrupting bone homeostasis.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"106153"},"PeriodicalIF":0.0,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Are hypoxia-related proteins associated with the invasiveness of glandular odontogenic cysts? A multicenter study.
Pub Date : 2024-11-30 DOI: 10.1016/j.archoralbio.2024.106151
Rafaela de Albuquerque Dias, Karolyny Martins Balbinot, Karine Duarte da Silva, Ana Paula Neutzling Gomes, Carla Mosconi, Elismauro Franscisco de Mendonça, Sandra Beatriz Chaves Tarquinio, Sérgio de Melo Alves Junior, Maria Cássia Ferreira de Aguiar, João de Jesus Viana Pinheiro

Objective: The study aimed to investigate the expression of hypoxia markers associated with invadopodia in glandular odontogenic cysts and to explore an association between this expression with the aggressive biological behaviour of this odontogenic cyst.

Design: Immunohistochemistry was employed to assess the expression of hypoxia-inducible factor 1 alpha (HIF-1α), notch homologous protein of the neurogenic locus 1 (NOTCH-1), disintegrin and metalloproteinase-12 (ADAM-12), and heparin-binding epidermal growth factor (HB-EGF) in 17 samples of glandular odontogenic cysts, 10 samples of calcifying odontogenic cysts, and 10 samples of dental follicles.

Results: The glandular odontogenic cyst samples exhibited increased expression of HIF-1α, NOTCH-1, ADAM-12 and HBEGF proteins compared with calcifying odontogenic cyst and dental follicle samples. HIF-1α demonstrated localization primarily within the nuclei of cystic epithelial cells of the glandular odontogenic cyst. NOTCH-1 and ADAM-12 exhibited expression in the cytoplasm and nuclei of epithelial and mucous cells of the glandular odontogenic cyst, of whereas HB-EGF was predominantly expressed in the cytoplasm. Weak labeling of these proteins was observed in the odontogenic epithelium of the calcifying odontogenic cyst and dental follicle samples.

Conclusions: The hypoxia-related signaling proteins are overexpressed in glandular odontogenic cyst when compared with calcifying odontogenic cyst and dental follicle. The reported aggressiveness of glandular odontogenic cyst can be partially explained by the expression of these proteins.

{"title":"Are hypoxia-related proteins associated with the invasiveness of glandular odontogenic cysts? A multicenter study.","authors":"Rafaela de Albuquerque Dias, Karolyny Martins Balbinot, Karine Duarte da Silva, Ana Paula Neutzling Gomes, Carla Mosconi, Elismauro Franscisco de Mendonça, Sandra Beatriz Chaves Tarquinio, Sérgio de Melo Alves Junior, Maria Cássia Ferreira de Aguiar, João de Jesus Viana Pinheiro","doi":"10.1016/j.archoralbio.2024.106151","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106151","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to investigate the expression of hypoxia markers associated with invadopodia in glandular odontogenic cysts and to explore an association between this expression with the aggressive biological behaviour of this odontogenic cyst.</p><p><strong>Design: </strong>Immunohistochemistry was employed to assess the expression of hypoxia-inducible factor 1 alpha (HIF-1α), notch homologous protein of the neurogenic locus 1 (NOTCH-1), disintegrin and metalloproteinase-12 (ADAM-12), and heparin-binding epidermal growth factor (HB-EGF) in 17 samples of glandular odontogenic cysts, 10 samples of calcifying odontogenic cysts, and 10 samples of dental follicles.</p><p><strong>Results: </strong>The glandular odontogenic cyst samples exhibited increased expression of HIF-1α, NOTCH-1, ADAM-12 and HBEGF proteins compared with calcifying odontogenic cyst and dental follicle samples. HIF-1α demonstrated localization primarily within the nuclei of cystic epithelial cells of the glandular odontogenic cyst. NOTCH-1 and ADAM-12 exhibited expression in the cytoplasm and nuclei of epithelial and mucous cells of the glandular odontogenic cyst, of whereas HB-EGF was predominantly expressed in the cytoplasm. Weak labeling of these proteins was observed in the odontogenic epithelium of the calcifying odontogenic cyst and dental follicle samples.</p><p><strong>Conclusions: </strong>The hypoxia-related signaling proteins are overexpressed in glandular odontogenic cyst when compared with calcifying odontogenic cyst and dental follicle. The reported aggressiveness of glandular odontogenic cyst can be partially explained by the expression of these proteins.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"106151"},"PeriodicalIF":0.0,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The activation level of the floor-of-the-mouth muscles is systematically modulated using tongue-pressing and swallowing tasks in healthy elderly.
Pub Date : 2024-11-30 DOI: 10.1016/j.archoralbio.2024.106155
Jong-Chi Oh

Objective: This study aimed to examine how muscle activity of the floor-of-the-mouth (FOM) muscles changes with different target exercise intensities of 40 %, 60 %, 80 %, and 100 % of maximum isometric pressure (MIP) during tongue-pressing and swallowing tasks in healthy elderly.

Design: This prospective, repeated-measures within-participant study included 35 participants (mean age: 75.2 ± 4.8 years, 26 women). Each participant performed 16 tasks using the Iowa Oral Performance Instrument (IOPI) in a randomized order, with two repetitions per task (anterior/posterior tongue × pressing/swallowing task × 40/60/80/100 % of the MIP). Furthermore, FOM-muscle activity during the task was simultaneously measured using surface electromyography. Statistical analysis was conducted using repeated-measures analysis of variance (ANOVA).

Results: Significant differences in FOM-muscle activity were observed across most intensity levels during pressing tasks (p < 0.001), with fewer significant differences noted during swallowing tasks, particularly involving the posterior tongue. A significant correlation was found between tongue pressure of the anterior/posterior tongue and FOM-muscle activation level (p < 0.001 and r = 0.517 and 0.447 for the isometric tongue pressure of the anterior and posterior tongue, respectively; r = 0.370 for the swallowing pressure of the anterior tongue) except for posterior tongue swallowing task.

Conclusions: These findings suggest that healthy elderly can systematically modulate suprahyoid muscle activity using a tongue-pressure-measurement device when performing accuracy training. However, they may require personalized systematic feedback and sufficient practice. The study underscores the potential of the IOPI in swallowing rehabilitation for elderly, while also highlighting the need for further research on patients with dysphagia.

{"title":"The activation level of the floor-of-the-mouth muscles is systematically modulated using tongue-pressing and swallowing tasks in healthy elderly.","authors":"Jong-Chi Oh","doi":"10.1016/j.archoralbio.2024.106155","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106155","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to examine how muscle activity of the floor-of-the-mouth (FOM) muscles changes with different target exercise intensities of 40 %, 60 %, 80 %, and 100 % of maximum isometric pressure (MIP) during tongue-pressing and swallowing tasks in healthy elderly.</p><p><strong>Design: </strong>This prospective, repeated-measures within-participant study included 35 participants (mean age: 75.2 ± 4.8 years, 26 women). Each participant performed 16 tasks using the Iowa Oral Performance Instrument (IOPI) in a randomized order, with two repetitions per task (anterior/posterior tongue × pressing/swallowing task × 40/60/80/100 % of the MIP). Furthermore, FOM-muscle activity during the task was simultaneously measured using surface electromyography. Statistical analysis was conducted using repeated-measures analysis of variance (ANOVA).</p><p><strong>Results: </strong>Significant differences in FOM-muscle activity were observed across most intensity levels during pressing tasks (p < 0.001), with fewer significant differences noted during swallowing tasks, particularly involving the posterior tongue. A significant correlation was found between tongue pressure of the anterior/posterior tongue and FOM-muscle activation level (p < 0.001 and r = 0.517 and 0.447 for the isometric tongue pressure of the anterior and posterior tongue, respectively; r = 0.370 for the swallowing pressure of the anterior tongue) except for posterior tongue swallowing task.</p><p><strong>Conclusions: </strong>These findings suggest that healthy elderly can systematically modulate suprahyoid muscle activity using a tongue-pressure-measurement device when performing accuracy training. However, they may require personalized systematic feedback and sufficient practice. The study underscores the potential of the IOPI in swallowing rehabilitation for elderly, while also highlighting the need for further research on patients with dysphagia.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"106155"},"PeriodicalIF":0.0,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multi-strain probiotic formula modulates expression of β-defensin-2, β-defensin-3, and TLR-4 in male rats with apical periodontitis.
Pub Date : 2024-11-29 DOI: 10.1016/j.archoralbio.2024.106137
Leopoldo Cosme-Silva, Renan Dal-Fabbro, Fernanda de Lima Pontes, Leticia Cabrera Capalbo, Edilson Ervolino, Luciano Tavares Angelo Cintra, Juan José Segura-Egea, João Eduardo Gomes-Filho

Objective: Evaluate whether a multi-strain probiotic formula affects blood parameters (hematologic, calcium, and phosphorus levels) and alters the expression of β-defensin-2, β-defensin-3, and toll-like receptor 4 in male rats with induced apical periodontitis (AP).

Design: Wistar rats were divided into two groups (n = 8 each): (1) rats with AP on a regular diet (Control) and (2) rats with AP on a regular diet supplemented with the multi-strain probiotic GNC Probiotic Complex (GCP) at one billion CFU. AP was induced by exposing the dental pulp of the first molars to the oral environment. GCP was administered daily via gavage for 30 days during AP development. After 30 days, animals were anesthetized, a cardiac puncture was performed, and 5 mL of blood was collected for hematologic, calcium, and phosphorus analysis. Animals were then euthanized, and mandibles were removed for histological and immunochemical analysis of β-defensin-2, β-defensin-3, and toll-like receptor 4. Statistical analyses used Mann-Whitney U and Student's t-tests, with significance at P < 0.05.

Results: No significant differences were observed in blood parameters between the Control and GCP groups (P > 0.05). In AP, the Control group showed more intense inflammatory infiltrates and higher median severity scores than the GCP group (P < 0.05). Immunoreactivity levels for β-defensin-2, β-defensin-3, and toll-like receptor 4 were significantly increased in the GCP group (P < 0.05).

Conclusion: Probiotic complex reduces inflammation and enhances immunolabeling of β-defensin-2, β-defensin-3, and toll-like receptor 4 in AP.

{"title":"Multi-strain probiotic formula modulates expression of β-defensin-2, β-defensin-3, and TLR-4 in male rats with apical periodontitis.","authors":"Leopoldo Cosme-Silva, Renan Dal-Fabbro, Fernanda de Lima Pontes, Leticia Cabrera Capalbo, Edilson Ervolino, Luciano Tavares Angelo Cintra, Juan José Segura-Egea, João Eduardo Gomes-Filho","doi":"10.1016/j.archoralbio.2024.106137","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106137","url":null,"abstract":"<p><strong>Objective: </strong>Evaluate whether a multi-strain probiotic formula affects blood parameters (hematologic, calcium, and phosphorus levels) and alters the expression of β-defensin-2, β-defensin-3, and toll-like receptor 4 in male rats with induced apical periodontitis (AP).</p><p><strong>Design: </strong>Wistar rats were divided into two groups (n = 8 each): (1) rats with AP on a regular diet (Control) and (2) rats with AP on a regular diet supplemented with the multi-strain probiotic GNC Probiotic Complex (GCP) at one billion CFU. AP was induced by exposing the dental pulp of the first molars to the oral environment. GCP was administered daily via gavage for 30 days during AP development. After 30 days, animals were anesthetized, a cardiac puncture was performed, and 5 mL of blood was collected for hematologic, calcium, and phosphorus analysis. Animals were then euthanized, and mandibles were removed for histological and immunochemical analysis of β-defensin-2, β-defensin-3, and toll-like receptor 4. Statistical analyses used Mann-Whitney U and Student's t-tests, with significance at P < 0.05.</p><p><strong>Results: </strong>No significant differences were observed in blood parameters between the Control and GCP groups (P > 0.05). In AP, the Control group showed more intense inflammatory infiltrates and higher median severity scores than the GCP group (P < 0.05). Immunoreactivity levels for β-defensin-2, β-defensin-3, and toll-like receptor 4 were significantly increased in the GCP group (P < 0.05).</p><p><strong>Conclusion: </strong>Probiotic complex reduces inflammation and enhances immunolabeling of β-defensin-2, β-defensin-3, and toll-like receptor 4 in AP.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"106137"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
m6A-related genes ALKBH5 and RBMX as prognostic and progression biomarkers in Chinese oral squamous cell carcinoma patients.
Pub Date : 2024-11-29 DOI: 10.1016/j.archoralbio.2024.106149
Yong Liu, Jiaying Nie, Ying Huang, Yunyan Yang, Wenen Su, Yumei Zhang, Zhuoqiao Gao, Shaohui Deng, Meilin Li, Shaoyan Lian, Jieying Li, Chaoqun Liu

Objective: N6-methyladenosine (m6A) RNA dysregulation is crucial for cancer development. The study aimed to explore the effects of m6A modification in oral squamous cell carcinoma (OSCC) and its potential as a biomarker and therapeutic target.

Design: We first analyzed m6A-related gene expression and its impact on OSCC prognosis and progression using the TCGA database. Subsequently, a Chinese cohort of 134 samples was used for validation. Bioinformatics analysis was conducted with TCGA data, and m6A levels were measured in the validation cohort using a quantification kit. Survival analysis was performed to study the relationship between m6A-related genes and OSCC prognosis in the Chinese population. The expression of m6A-related genes was assessed by using quantitative real-time PCR, Western blot analysis, and immunohistochemistry.

Results: In the TCGA database, we found dysregulated expressions of METTL14, ALKBH5, YTHDF2, HNRNPC, LRPPRC, HNRNPA2B1, IGF2BP2, and RBMX in OSCC. Based on this, we observed significantly elevated total m6A content in OSCC tissues compared to normal controls in the validation cohort. Among the m6A candidate genes, only ALKBH5 and RBMX upregulation were found to be independent prognostic risk factors for poor OSCC survival in the Chinese population. And the inclusion of these two genes had a higher area under the curve for 3-year (0.705, 0.826), and 5-year (0.715, 0.788) overall survival compared to the model that only considered clinical parameters.

Conclusions: We found the upregulation of m6A status in OSCC, of which, ALKBH5 and RBMX may serve as promising diagnostic and prognostic biomarkers for Chinese patients with OSCC.

{"title":"m6A-related genes ALKBH5 and RBMX as prognostic and progression biomarkers in Chinese oral squamous cell carcinoma patients.","authors":"Yong Liu, Jiaying Nie, Ying Huang, Yunyan Yang, Wenen Su, Yumei Zhang, Zhuoqiao Gao, Shaohui Deng, Meilin Li, Shaoyan Lian, Jieying Li, Chaoqun Liu","doi":"10.1016/j.archoralbio.2024.106149","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106149","url":null,"abstract":"<p><strong>Objective: </strong>N6-methyladenosine (m6A) RNA dysregulation is crucial for cancer development. The study aimed to explore the effects of m6A modification in oral squamous cell carcinoma (OSCC) and its potential as a biomarker and therapeutic target.</p><p><strong>Design: </strong>We first analyzed m6A-related gene expression and its impact on OSCC prognosis and progression using the TCGA database. Subsequently, a Chinese cohort of 134 samples was used for validation. Bioinformatics analysis was conducted with TCGA data, and m6A levels were measured in the validation cohort using a quantification kit. Survival analysis was performed to study the relationship between m6A-related genes and OSCC prognosis in the Chinese population. The expression of m6A-related genes was assessed by using quantitative real-time PCR, Western blot analysis, and immunohistochemistry.</p><p><strong>Results: </strong>In the TCGA database, we found dysregulated expressions of METTL14, ALKBH5, YTHDF2, HNRNPC, LRPPRC, HNRNPA2B1, IGF2BP2, and RBMX in OSCC. Based on this, we observed significantly elevated total m6A content in OSCC tissues compared to normal controls in the validation cohort. Among the m6A candidate genes, only ALKBH5 and RBMX upregulation were found to be independent prognostic risk factors for poor OSCC survival in the Chinese population. And the inclusion of these two genes had a higher area under the curve for 3-year (0.705, 0.826), and 5-year (0.715, 0.788) overall survival compared to the model that only considered clinical parameters.</p><p><strong>Conclusions: </strong>We found the upregulation of m6A status in OSCC, of which, ALKBH5 and RBMX may serve as promising diagnostic and prognostic biomarkers for Chinese patients with OSCC.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"106149"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tensile stress induced osteogenesis of periodontal ligament cells via Piezo1 mediated TAZ-Cbfα1 signaling.
Pub Date : 2024-11-29 DOI: 10.1016/j.archoralbio.2024.106152
Sisi Li, Ting Jin, Yi Wang, Hui Deng, Rongdang Hu

Objective: Cyclic tensile stress (CTS) is known to induce osteogenesis of periodontal ligament cells (PDLCs), in which the molecular mechanism remains to be elucidated. This study aimed to investigate the role of the mechanosensitive calcium channel Piezo1 in the osteogenesis of PDLCs under tensile strain, as well as the signal regulation of the TAZ-Cbfα1 pathway in this process.

Design: PDLCs were isolated from periodontal ligament tissues and subjected to CTS. Alizarin red staining (ARS) and alkaline phosphatase (ALP) assay were used to detect the osteogenesis of PDLCs. RT-qPCR and Western blot were used to detect the transcripts and protein expression levels of Piezo1, Transcriptional co-activator with PDZ binding motif (TAZ), and Core-binding factor α1 (Cbfα1) respectively. Immunofluorescence staining was used to detect the nuclear aggregation of TAZ. Small interfering RNA (siRNA) targeting Piezo1 (Piezo1-siRNA) was adopted to inhibit Piezo1 mRNA expression.

Results: The results showed that the osteogenic differentiation capacity of PDLCs was significantly enhanced under CTS, along with elevated mRNA and protein expression levels of Piezo1, TAZ, and Cbfα1. Moreover, the ALP activity and the formation of calcium nodules by ARS staining were significantly increased. In addition, Piezo1 siRNA infection significantly inhibited the CTS-induced TAZ-Cbfα1 pathway and the osteogenesis of PDLCs, suggesting the regulatory role of Piezo1.

Conclusions: We provided evidence that the application of CTS encourages the osteogenic differentiation of PDLCs, which could be mediated by the Piezo1 targeted TAZ-Cbfα1 signaling.

目的:已知循环拉伸应力(CTS)可诱导牙周韧带细胞(PDLCs)成骨,其分子机制尚待阐明。本研究旨在探讨机械敏感性钙通道Piezo1在拉伸应变下PDLCs成骨过程中的作用,以及TAZ-Cbfα1通路在此过程中的信号调控:设计:从牙周韧带组织中分离出PDLCs,并对其进行CTS处理。采用茜素红染色法(ARS)和碱性磷酸酶(ALP)检测PDLCs的成骨过程。RT-qPCR和Western印迹法分别检测Piezo1、具有PDZ结合基调的转录共激活因子(TAZ)和核心结合因子α1(Cbfα1)的转录本和蛋白表达水平。免疫荧光染色用于检测 TAZ 的核聚集。采用靶向Piezo1的小干扰RNA(siRNA)抑制Piezo1 mRNA的表达:结果表明:在CTS作用下,PDLCs的成骨分化能力显著增强,Piezo1、TAZ和Cbfα1的mRNA和蛋白表达水平升高。此外,ALP活性和ARS染色钙结节的形成也明显增加。此外,Piezo1 siRNA感染可明显抑制CTS诱导的TAZ-Cbfα1通路和PDLCs的成骨过程,表明Piezo1具有调控作用:我们提供的证据表明,应用CTS可促进PDLCs的成骨分化,而这可能是由Piezo1靶向TAZ-Cbfα1信号传导介导的。
{"title":"Tensile stress induced osteogenesis of periodontal ligament cells via Piezo1 mediated TAZ-Cbfα1 signaling.","authors":"Sisi Li, Ting Jin, Yi Wang, Hui Deng, Rongdang Hu","doi":"10.1016/j.archoralbio.2024.106152","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106152","url":null,"abstract":"<p><strong>Objective: </strong>Cyclic tensile stress (CTS) is known to induce osteogenesis of periodontal ligament cells (PDLCs), in which the molecular mechanism remains to be elucidated. This study aimed to investigate the role of the mechanosensitive calcium channel Piezo1 in the osteogenesis of PDLCs under tensile strain, as well as the signal regulation of the TAZ-Cbfα1 pathway in this process.</p><p><strong>Design: </strong>PDLCs were isolated from periodontal ligament tissues and subjected to CTS. Alizarin red staining (ARS) and alkaline phosphatase (ALP) assay were used to detect the osteogenesis of PDLCs. RT-qPCR and Western blot were used to detect the transcripts and protein expression levels of Piezo1, Transcriptional co-activator with PDZ binding motif (TAZ), and Core-binding factor α1 (Cbfα1) respectively. Immunofluorescence staining was used to detect the nuclear aggregation of TAZ. Small interfering RNA (siRNA) targeting Piezo1 (Piezo1-siRNA) was adopted to inhibit Piezo1 mRNA expression.</p><p><strong>Results: </strong>The results showed that the osteogenic differentiation capacity of PDLCs was significantly enhanced under CTS, along with elevated mRNA and protein expression levels of Piezo1, TAZ, and Cbfα1. Moreover, the ALP activity and the formation of calcium nodules by ARS staining were significantly increased. In addition, Piezo1 siRNA infection significantly inhibited the CTS-induced TAZ-Cbfα1 pathway and the osteogenesis of PDLCs, suggesting the regulatory role of Piezo1.</p><p><strong>Conclusions: </strong>We provided evidence that the application of CTS encourages the osteogenic differentiation of PDLCs, which could be mediated by the Piezo1 targeted TAZ-Cbfα1 signaling.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"106152"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Truncated recombinant Jagged1 fused with human IgG1 Fc activates Notch target genes in human periodontal ligament cells.
Pub Date : 2024-11-28 DOI: 10.1016/j.archoralbio.2024.106138
Y Nhu Tran, Ajjima Chansaenroj, Araya Jivaphetthai, Thanaphum Osathanon, Wanatchaporn Arunmanee

Objective: Jagged1, a Notch ligand, is essential for osteogenic differentiation in human periodontal ligament cells (hPDLs) by interacting with Notch2 to induce osteogenic markers, alkaline phosphatase activity, and mineral deposition. However, its large size hampers absorption and distribution of biomaterials. This study aimed to identify the critical region of Jagged1 necessary for its interaction with Notch2 to create a truncated version that retains osteogenic activity but with improved delivery characteristics.

Methods: Truncated versions of Jagged1 were designed by deleting C-terminal regions, focusing on the importance of the N-terminal domain. Both truncated and full-length Jagged1 were fused with human IgG1 Fc (Jagged1-Fc) and expressed in Chinese hamster ovary cells. hPDLs treated with these constructs were analyzed for Notch target gene expression using real-time PCR. Mineral deposition was assessed using alizarin red S staining.

Results: Both truncated and full-length Jagged1-Fc increased the expression of Notch target genes (Hes1, Hey1, and ALP) in hPDLs, indicating successful activation of Notch signaling. However, only the full-length Jagged1-Fc enhanced mineral deposition, while the truncated version did not.

Conclusions: Full-length Jagged1-Fc is required for mineral deposition and complete osteogenic differentiation in hPDLs. The truncated versions, while capable of activating Notch signaling, are ineffective in promoting mineralization, underscoring the importance of the entire protein for clinical applications in bone regeneration.

{"title":"Truncated recombinant Jagged1 fused with human IgG1 Fc activates Notch target genes in human periodontal ligament cells.","authors":"Y Nhu Tran, Ajjima Chansaenroj, Araya Jivaphetthai, Thanaphum Osathanon, Wanatchaporn Arunmanee","doi":"10.1016/j.archoralbio.2024.106138","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106138","url":null,"abstract":"<p><strong>Objective: </strong>Jagged1, a Notch ligand, is essential for osteogenic differentiation in human periodontal ligament cells (hPDLs) by interacting with Notch2 to induce osteogenic markers, alkaline phosphatase activity, and mineral deposition. However, its large size hampers absorption and distribution of biomaterials. This study aimed to identify the critical region of Jagged1 necessary for its interaction with Notch2 to create a truncated version that retains osteogenic activity but with improved delivery characteristics.</p><p><strong>Methods: </strong>Truncated versions of Jagged1 were designed by deleting C-terminal regions, focusing on the importance of the N-terminal domain. Both truncated and full-length Jagged1 were fused with human IgG1 Fc (Jagged1-Fc) and expressed in Chinese hamster ovary cells. hPDLs treated with these constructs were analyzed for Notch target gene expression using real-time PCR. Mineral deposition was assessed using alizarin red S staining.</p><p><strong>Results: </strong>Both truncated and full-length Jagged1-Fc increased the expression of Notch target genes (Hes1, Hey1, and ALP) in hPDLs, indicating successful activation of Notch signaling. However, only the full-length Jagged1-Fc enhanced mineral deposition, while the truncated version did not.</p><p><strong>Conclusions: </strong>Full-length Jagged1-Fc is required for mineral deposition and complete osteogenic differentiation in hPDLs. The truncated versions, while capable of activating Notch signaling, are ineffective in promoting mineralization, underscoring the importance of the entire protein for clinical applications in bone regeneration.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"106138"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibacterial and antibiofilm potential of Thuja orientalis L. extract targeting cariogenic Enterococcus faecalis ATCC 29212: A combined in-vitro, in-silico study, and cytotoxicity screening.
Pub Date : 2024-10-10 DOI: 10.1016/j.archoralbio.2024.106107
Khyati Koul, Ishwerpreet Kaur Jawanda, Thomson Soni, Kashish Madaan, Sunidhi Bhatt, Pranjali Singh, Divyani Sharma, Sonia Bhonchal Bhardwaj, Seema Kumari

Objectives: In this study, we explored the efficacy of methanolic extract of Thuja orientalis (TOME) as a novel antibacterial and antibiofilm agent against a cariogenic bacterium, Enterococcus faecalis ATCC 29212.

Design: Antibacterial susceptibility studies were conducted and surface morphology analysis was performed using field emission scanning electron microscopy (FESEM). Antibiofilm activity was evaluated through both qualitative and quantitative biofilm inhibition assays and validated by microscopic analysis. In-silico molecular docking studies were conducted using the EDock server. The effectiveness of TOME was substantiated by biofilm model on dentin discs and cytotoxicity towards the HaCaT cell line was assessed using the MTT assay.

Results: TOME exhibited significant bactericidal activity with minimum inhibitory concentration of 12.5 mg/mL and additionally, it effectively compromised bacterial cell wall integrity. Qualitative, quantitative and microscopic studies depicted the inhibition of biofilm formation. TOME significantly impacted the production of extracellular polymeric substance and extracellular DNA. Molecular docking studies identified beta-caryophyllene as a potent inhibitor of the Enterococcal surface protein (Esp). Biofilm model depicted the reduction of bacterial load on dentin discs. Additionally, TOME showed reduced cytotoxicity on HaCaT cells, indicating its potential as a safe therapeutic agent.

Conclusion: These findings highlight TOME's promise for developing novel treatments for dental infections and biofilm-associated diseases. Further research should focus on isolating and characterizing the active compounds within TOME, particularly beta-caryophyllene, to elucidate their precise mechanisms of action.

{"title":"Antibacterial and antibiofilm potential of Thuja orientalis L. extract targeting cariogenic Enterococcus faecalis ATCC 29212: A combined in-vitro, in-silico study, and cytotoxicity screening.","authors":"Khyati Koul, Ishwerpreet Kaur Jawanda, Thomson Soni, Kashish Madaan, Sunidhi Bhatt, Pranjali Singh, Divyani Sharma, Sonia Bhonchal Bhardwaj, Seema Kumari","doi":"10.1016/j.archoralbio.2024.106107","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106107","url":null,"abstract":"<p><strong>Objectives: </strong>In this study, we explored the efficacy of methanolic extract of Thuja orientalis (TOME) as a novel antibacterial and antibiofilm agent against a cariogenic bacterium, Enterococcus faecalis ATCC 29212.</p><p><strong>Design: </strong>Antibacterial susceptibility studies were conducted and surface morphology analysis was performed using field emission scanning electron microscopy (FESEM). Antibiofilm activity was evaluated through both qualitative and quantitative biofilm inhibition assays and validated by microscopic analysis. In-silico molecular docking studies were conducted using the EDock server. The effectiveness of TOME was substantiated by biofilm model on dentin discs and cytotoxicity towards the HaCaT cell line was assessed using the MTT assay.</p><p><strong>Results: </strong>TOME exhibited significant bactericidal activity with minimum inhibitory concentration of 12.5 mg/mL and additionally, it effectively compromised bacterial cell wall integrity. Qualitative, quantitative and microscopic studies depicted the inhibition of biofilm formation. TOME significantly impacted the production of extracellular polymeric substance and extracellular DNA. Molecular docking studies identified beta-caryophyllene as a potent inhibitor of the Enterococcal surface protein (Esp). Biofilm model depicted the reduction of bacterial load on dentin discs. Additionally, TOME showed reduced cytotoxicity on HaCaT cells, indicating its potential as a safe therapeutic agent.</p><p><strong>Conclusion: </strong>These findings highlight TOME's promise for developing novel treatments for dental infections and biofilm-associated diseases. Further research should focus on isolating and characterizing the active compounds within TOME, particularly beta-caryophyllene, to elucidate their precise mechanisms of action.</p>","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"106107"},"PeriodicalIF":0.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel mutation in GPR68 causes hypomaturation amelogenesis imperfecta GPR68的一种新型突变会导致发育不全髓质发育不全症
Pub Date : 2024-05-01 DOI: 10.1016/j.archoralbio.2024.105991
Shunlan Yu, Dandan Liu, Changqing Yan, Chao Yuan, Chenying Zhang, Shuguo Zheng
{"title":"A novel mutation in GPR68 causes hypomaturation amelogenesis imperfecta","authors":"Shunlan Yu, Dandan Liu, Changqing Yan, Chao Yuan, Chenying Zhang, Shuguo Zheng","doi":"10.1016/j.archoralbio.2024.105991","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105991","url":null,"abstract":"","PeriodicalId":93882,"journal":{"name":"Archives of oral biology","volume":"111 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141033312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Archives of oral biology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1