Pub Date : 2009-12-28DOI: 10.3760/CMA.J.ISSN.1006-9801.2009.12.003
Dong Zhi-ming
OBJECTIVE:We investigated the promoter methylation of secreted frizzled-related protein 4(SFRP4) and SFRP5 gene in gastric cardia adenocarcinoma (GCA).METHODS:Methylation specific PCR (MSP) method was used to examine the methylation status of the 5' CpG island of SFRP4 and SFRP5 genes in 94 tumors and 47 corresponding normal tissues.RESULTS:Methylation frequencies of SFRP4 and SFRP5 genes in tumors were 68.1%(64/94)and 79.8%(75/94),respectively,significantly higher than that in corresponding normal tissues (8.5% and 12.8%,respectively)(P0.01).Methylation frequencies of SFRP4 in poor differentiation group (92.6%,50/54)was significantly higher than that in moderate and poor-moderate differentiation groups (35%,14/40).Methylation frequencies of SFRP5 in lymph node metastasis group (89.3%,50/56) was significantly higher than that in no lymph node metastasis group (65.8%,25/38).Methylation frequencies of SFRP5 in poor differentiation group was higher than that in moderate and poor-moderate differentiation groups,and SFRP4 in lymph node metastasis group was higher than that in no lymph node metastasis group but without significant difference(P0.05).57 cases of GCA showed simultaneous methylation of SFRP4 and SFRP5 genes,including 18 moderate and poor-moderate differentiation tumors,and 39 moderate differentiation tumors.Simultaneous methylation frequencies of SFRP4 and SFRP5 genes in moderate and poor-moderate differentiation groups was significantly lower than that in moderate group.CONCLUSION:SFRP4 and SFRP5 genes might be associated with oncogenanesis of GCA and hypermethylation of these genes might be related to the malignant behavior of GCA.
目的:研究分泌性卷曲相关蛋白4(SFRP4)和SFRP5基因在贲门腺癌(GCA)中的启动子甲基化情况。方法:采用甲基化特异性PCR (Methylation specific PCR, MSP)方法检测94例肿瘤和47例正常组织中SFRP4和SFRP5基因5′CpG岛的甲基化状态。结果:肿瘤组织中SFRP4和SFRP5基因甲基化频率分别为68.1%(64/94)和79.8%(75/94)，显著高于相应的正常组织(8.5%和12.8%)(P0.01)。低分化组SFRP4的甲基化频率(92.6%，50/54)显著高于中分化组和中差分化组(35%，14/40)。淋巴结转移组SFRP5甲基化频率(89.3%，50/56)显著高于无淋巴结转移组(65.8%，25/38)。SFRP5在低分化组中甲基化频率高于中度和中差分化组，SFRP4在淋巴结转移组中甲基化频率高于无淋巴结转移组，但差异无统计学意义(P0.05)。57例GCA同时出现SFRP4和SFRP5基因甲基化，其中中度和低中度分化肿瘤18例，中度分化肿瘤39例。中度分化组和欠中度分化组SFRP4和SFRP5基因同时甲基化频率显著低于中度分化组。结论:SFRP4和SFRP5基因可能与GCA的癌变有关，其高甲基化可能与GCA的恶性行为有关。
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