Rapidly expanding cities with simultaneous increase in the population have put immense pressure on the existing green spaces and biodiversity in the cities. The increasing air pollution, high carbon emissions, and increased intensity of flash floods and urban heat island effects in the fast-growing metropolitan cities of India especially National Capital Territory (NCT) Delhi have put an alert on the human well-being of the urban residents. Planning and implementing site-specific and cost-effective nature-based solutions become a prerequisite for improving livability and developing resilient cities as per Sustainable Development Goal (SDG) 11. ‘City Biodiversity Index’ (CBI) was assessed to understand the biodiversity concerns, efforts, and challenges for the city. The assessment utilized recently upgraded 28 indicators of the CBI following a standard protocol outlined in the CBI manual, which incorporated elements of remote sensing and literature review. For the first time, Delhi’s CBI was evaluated resulting in a score of 57 out of 100 points. The CBI was divided into three components: native biodiversity (including flora and fauna), ecosystem services, and governance and management, which scored 10 out of 24 points, 5 out of 20 points, and 42 out of 56 points, respectively. These CBI scores serve as critical indicators that demand attention from both state and central governments. With a score slightly above 50%, it is crucial for the city to prioritize biodiversity conservation and incorporate the research findings into the local biodiversity action plan.