Blood-group substance A from pooled hog-stomach linings was subjected to hydrolysis by a protease from asian influenza virus and to hydroxylaminolysis. Half of the polymer appeared in the dialyzable fraction after proteolysis. The non-dialyzable polysaccharide fragment retained only aspartic acid. Hydroxylaminolysis results in 30% yield of dialyzable products. The non-dialyzable fragment contained no aspartic acid after this cleavage. Mono- and oligosaccharides, peptides and short-chain glycopeptides were detected among the dialyzable products of hydroxylaminolysis. On the basis of the results obtained, a general plan of the structure of blood-group substance A is proposed.
{"title":"Structural studies of blood-group substance A from hog-stomach linings","authors":"N.K. Kochetkov, V.A. Derevitskaya, S.G. Kara-Murza, V.G. Zharov","doi":"10.1016/0926-6526(64)90050-3","DOIUrl":"10.1016/0926-6526(64)90050-3","url":null,"abstract":"<div><p>Blood-group substance A from pooled hog-stomach linings was subjected to hydrolysis by a protease from asian influenza virus and to hydroxylaminolysis. Half of the polymer appeared in the dialyzable fraction after proteolysis. The non-dialyzable polysaccharide fragment retained only aspartic acid. Hydroxylaminolysis results in 30% yield of dialyzable products. The non-dialyzable fragment contained no aspartic acid after this cleavage. Mono- and oligosaccharides, peptides and short-chain glycopeptides were detected among the dialyzable products of hydroxylaminolysis. On the basis of the results obtained, a general plan of the structure of blood-group substance A is proposed.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 52-60"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90050-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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