Pub Date : 2015-05-18DOI: 10.3969/j.issn.1007-3969.2015.03.003
L. Yuanyuan, W. Hongyan, W. Xiaoyan
{"title":"Effects of progranulin on proliferation and senescence in gastric cancer cells","authors":"L. Yuanyuan, W. Hongyan, W. Xiaoyan","doi":"10.3969/j.issn.1007-3969.2015.03.003","DOIUrl":"https://doi.org/10.3969/j.issn.1007-3969.2015.03.003","url":null,"abstract":"","PeriodicalId":10041,"journal":{"name":"China Oncology","volume":"25 1","pages":"173-178"},"PeriodicalIF":0.0,"publicationDate":"2015-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70178395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-18DOI: 10.3969/j.issn.1007-3969.2015.03.001
Ni Xiaochen, Zhao Zhihong, Ma Yongliang
{"title":"miR-222 can inhibit the autophagy of renal cell carcinoma cells through down-regulating the expression of DDIT4","authors":"Ni Xiaochen, Zhao Zhihong, Ma Yongliang","doi":"10.3969/j.issn.1007-3969.2015.03.001","DOIUrl":"https://doi.org/10.3969/j.issn.1007-3969.2015.03.001","url":null,"abstract":"","PeriodicalId":10041,"journal":{"name":"China Oncology","volume":"1 1","pages":"161-166"},"PeriodicalIF":0.0,"publicationDate":"2015-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70178758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-08DOI: 10.3969/j.issn.1007-3969.2015.01.001
Wang Lingzhi, Peng Jianxin, Tao Liang
{"title":"The role of gap junctions composed of connexin 43 on the anti-tumor effect induced by etoposide","authors":"Wang Lingzhi, Peng Jianxin, Tao Liang","doi":"10.3969/j.issn.1007-3969.2015.01.001","DOIUrl":"https://doi.org/10.3969/j.issn.1007-3969.2015.01.001","url":null,"abstract":"","PeriodicalId":10041,"journal":{"name":"China Oncology","volume":"25 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2015-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70178154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-08DOI: 10.3969/J.ISSN.1007-3969.2015.01.007
L. Min, Fang Mingzhi
Background and purpose: Oxaliplatin(L-OHP) is one of the most commonly used chemotherapy drugs in colorectal cancer and L-OHP-resistance is very common in colorectal cancer therapy. This research was to discuss the reversal effect in L-OHP-resistant human colon cancer cell line Lo Vo/L-OHP by anemoside B4(AB4) and tetrandrine(Tet) and to clarify their molecular mechanism. Methods: Lo Vo/L-OHP cells were treated for 48 h by AB4 and Tet at different concentrations to get non-toxic dose. Drug sensitivity was measured by MTT. After the treatment, the cell cycle and apoptosis of the cells were detected. Expression of P-gp m RNA, z DHHC9 m RNA and SMAD4 m RNA were detected by RT-PCR. Expression of P-gp, z DHHC9 and SMAD4 protein were detected by Western blot. Results: The IC50 of Lo Vo/L-OHP for L-OHP was(112.5±23.6) μg/m L, and the IC50 decreased to(62.8±21.4) μg/m L(P0.05) and(58.9±26.3) μg/m L(P0.05) after the treatment with 0.71 μg/m L AB4 and 0.45 μg/m L Tet(non-toxic dose) separately. The cell cycle experiment showed that the cells in G1 stage decreased and in S stage increased but with no statistical significance(P0.05). The cell apoptosis experiment showed that the apoptotic rate increased after the treatment with AB4 and Tet with non-toxic dose but with no statistical significance(P0.05). The RT-PCR experiment showed that the expressions of P-gp m RNA and z DHHC9 m RNA were increased and SMAD4 m RNA was decreased in Lo Vo/L-OHP cells compared with in Lo Vo cells(P0.05), while it was found that P-gp m RNA in Lo Vo/L-OHP cells was decreased after the treatment with AB4 at non-toxic dose(P0.05). Western blot experiment showed the protein of P-gp in Lo Vo/L-OHP cells was decreased after treatment with AB4(P0.05), which was accordant to the PCR result. The expression of z DHHC9 protein in Lo Vo/L-OHP cells was decreased in Lo Vo/L-OHP cells after treatment with Tet(P0.05). Conclusion: AB4 and Tet have some reversal effect on resistant to L-OHP in Lo Vo/L-OHP cells. The molecular mechanism of the resistance reverse effect was related to down-regulation of P-gp for AB4 and down-regulation of z DHHC9 for Tet.
背景与目的:奥沙利铂(Oxaliplatin, L-OHP)是结直肠癌最常用的化疗药物之一，L-OHP耐药在结直肠癌治疗中十分常见。本研究旨在探讨anemoside B4(AB4)和粉防己碱(Tet)对L-OHP耐药人结肠癌细胞株Lo Vo/L-OHP的逆转作用，并阐明其分子机制。方法:以不同浓度的AB4和Tet作用于Lo Vo/L-OHP细胞48 h，获得无毒剂量。采用MTT法测定药物敏感性。处理后检测细胞周期和细胞凋亡情况。RT-PCR检测P-gp m RNA、z - DHHC9 m RNA和SMAD4 m RNA的表达。Western blot检测P-gp、z DHHC9和SMAD4蛋白的表达。结果:经0.71 μg/m L AB4和0.45 μg/m L Tet(无毒剂量)处理后，Lo Vo/L- ohp对L- ohp的IC50分别为(112.5±23.6)μg/m L和(62.8±21.4)μg/m L(P0.05)和(58.9±26.3)μ L(P0.05)。细胞周期实验显示G1期细胞数量减少，S期细胞数量增加，但差异无统计学意义(P0.05)。细胞凋亡实验显示，无毒剂量的AB4和Tet处理后，细胞凋亡率升高，但无统计学意义(P0.05)。RT-PCR实验显示，与Lo Vo细胞相比，Lo Vo/L-OHP细胞中P-gp m RNA和z - DHHC9 m RNA的表达增加(P0.05)， SMAD4 m RNA的表达减少(P0.05)，而无毒剂量AB4处理后，Lo Vo/L-OHP细胞中P-gp m RNA的表达减少(P0.05)。Western blot实验显示，AB4处理后，Lo Vo/L-OHP细胞中P-gp蛋白含量降低(P0.05)，与PCR结果一致。Tet作用后，Lo Vo/L-OHP细胞中z DHHC9蛋白表达降低(P0.05)。结论:AB4和Tet对Lo Vo/L-OHP细胞的L-OHP耐药有一定的逆转作用。抗性逆转作用的分子机制与P-gp对AB4的下调和z DHHC9对Tet的下调有关。
{"title":"Resistance reverse effect of anemoside B4 and tetrandrine on oxaliplatin-resistant human colon cancer cells and the mechanism","authors":"L. Min, Fang Mingzhi","doi":"10.3969/J.ISSN.1007-3969.2015.01.007","DOIUrl":"https://doi.org/10.3969/J.ISSN.1007-3969.2015.01.007","url":null,"abstract":"Background and purpose: Oxaliplatin(L-OHP) is one of the most commonly used chemotherapy drugs in colorectal cancer and L-OHP-resistance is very common in colorectal cancer therapy. This research was to discuss the reversal effect in L-OHP-resistant human colon cancer cell line Lo Vo/L-OHP by anemoside B4(AB4) and tetrandrine(Tet) and to clarify their molecular mechanism. Methods: Lo Vo/L-OHP cells were treated for 48 h by AB4 and Tet at different concentrations to get non-toxic dose. Drug sensitivity was measured by MTT. After the treatment, the cell cycle and apoptosis of the cells were detected. Expression of P-gp m RNA, z DHHC9 m RNA and SMAD4 m RNA were detected by RT-PCR. Expression of P-gp, z DHHC9 and SMAD4 protein were detected by Western blot. Results: The IC50 of Lo Vo/L-OHP for L-OHP was(112.5±23.6) μg/m L, and the IC50 decreased to(62.8±21.4) μg/m L(P0.05) and(58.9±26.3) μg/m L(P0.05) after the treatment with 0.71 μg/m L AB4 and 0.45 μg/m L Tet(non-toxic dose) separately. The cell cycle experiment showed that the cells in G1 stage decreased and in S stage increased but with no statistical significance(P0.05). The cell apoptosis experiment showed that the apoptotic rate increased after the treatment with AB4 and Tet with non-toxic dose but with no statistical significance(P0.05). The RT-PCR experiment showed that the expressions of P-gp m RNA and z DHHC9 m RNA were increased and SMAD4 m RNA was decreased in Lo Vo/L-OHP cells compared with in Lo Vo cells(P0.05), while it was found that P-gp m RNA in Lo Vo/L-OHP cells was decreased after the treatment with AB4 at non-toxic dose(P0.05). Western blot experiment showed the protein of P-gp in Lo Vo/L-OHP cells was decreased after treatment with AB4(P0.05), which was accordant to the PCR result. The expression of z DHHC9 protein in Lo Vo/L-OHP cells was decreased in Lo Vo/L-OHP cells after treatment with Tet(P0.05). Conclusion: AB4 and Tet have some reversal effect on resistant to L-OHP in Lo Vo/L-OHP cells. The molecular mechanism of the resistance reverse effect was related to down-regulation of P-gp for AB4 and down-regulation of z DHHC9 for Tet.","PeriodicalId":10041,"journal":{"name":"China Oncology","volume":"25 1","pages":"38-44"},"PeriodicalIF":0.0,"publicationDate":"2015-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70178298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-07DOI: 10.3969/J.ISSN.1007-3969.2014.12.005
Wu Chun-tao, L. Liang, Xu Yong-feng
{"title":"Effect of lienal polypeptide injection on cellular immunity of patients with chemotherapy after pancreatic radical resection","authors":"Wu Chun-tao, L. Liang, Xu Yong-feng","doi":"10.3969/J.ISSN.1007-3969.2014.12.005","DOIUrl":"https://doi.org/10.3969/J.ISSN.1007-3969.2014.12.005","url":null,"abstract":"","PeriodicalId":10041,"journal":{"name":"China Oncology","volume":"24 1","pages":"906-913"},"PeriodicalIF":0.0,"publicationDate":"2015-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70178131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-07DOI: 10.3969/j.issn.1007-3969.2014.12.011
L. Yi, Lv Yan-li, Zhao Yue
{"title":"Preliminary analysis of different organization models of breast cancer screening","authors":"L. Yi, Lv Yan-li, Zhao Yue","doi":"10.3969/j.issn.1007-3969.2014.12.011","DOIUrl":"https://doi.org/10.3969/j.issn.1007-3969.2014.12.011","url":null,"abstract":"","PeriodicalId":10041,"journal":{"name":"China Oncology","volume":"24 1","pages":"944-950"},"PeriodicalIF":0.0,"publicationDate":"2015-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70178286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-07DOI: 10.3969/J.ISSN.1007-3969.2014.12.012
Shao Yu-hui, F. Jie
{"title":"Progress in research of adaptive radiation therapy for head and neck cancer","authors":"Shao Yu-hui, F. Jie","doi":"10.3969/J.ISSN.1007-3969.2014.12.012","DOIUrl":"https://doi.org/10.3969/J.ISSN.1007-3969.2014.12.012","url":null,"abstract":"","PeriodicalId":10041,"journal":{"name":"China Oncology","volume":"24 1","pages":"951-955"},"PeriodicalIF":0.0,"publicationDate":"2015-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70177999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-05DOI: 10.3969/J.ISSN.1007-3969.2014.11.002
Fu Deyuan, Ren Chuanli, Tan Hao-sheng, Wei Jin-li, Zhu Yu-xiang, He Chun-lan, Shao Wen-xi, Zhang Jia-xin
{"title":"Clinical significance of Sox17 gene promoter methylation in plasma circulating DNA in breast cancer patients","authors":"Fu Deyuan, Ren Chuanli, Tan Hao-sheng, Wei Jin-li, Zhu Yu-xiang, He Chun-lan, Shao Wen-xi, Zhang Jia-xin","doi":"10.3969/J.ISSN.1007-3969.2014.11.002","DOIUrl":"https://doi.org/10.3969/J.ISSN.1007-3969.2014.11.002","url":null,"abstract":"","PeriodicalId":10041,"journal":{"name":"China Oncology","volume":"24 1","pages":"808-813"},"PeriodicalIF":0.0,"publicationDate":"2015-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70177491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-05DOI: 10.3969/j.issn.1007-3969.2014.11.003
Fan Xingwen, Guan Shi-kuo, Wu Kailiang
{"title":"The effect of brain irradiation on mood and memory for rats","authors":"Fan Xingwen, Guan Shi-kuo, Wu Kailiang","doi":"10.3969/j.issn.1007-3969.2014.11.003","DOIUrl":"https://doi.org/10.3969/j.issn.1007-3969.2014.11.003","url":null,"abstract":"","PeriodicalId":10041,"journal":{"name":"China Oncology","volume":"24 1","pages":"814-819"},"PeriodicalIF":0.0,"publicationDate":"2015-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70177824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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