Medicine in Omics最新文献

{"title":"Exploring the clinical and pharmacotherapeutic implications of druggable genome signaling in lung adenocarcinoma: An integrated approach with transcriptome and Mendelian randomization analysis","authors":"Guanrong Li ,&nbsp;Xiao Zhu","doi":"10.1016/j.meomic.2025.100053","DOIUrl":"10.1016/j.meomic.2025.100053","url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to improve prognostic models and therapeutic targets for lung adenocarcinoma (LUAD) and explore possible tumor microenvironmental pathways. We focused on long non-coding RNAs (lncRNAs), which have been implicated in LUAD progression.</div></div><div><h3>Methods</h3><div>Using data from The Cancer Genome Atlas (TCGA), we developed a prognostic model based on druggable genome-associated lncRNAs. Rigorous validation confirmed its predictive accuracy. We identified Druggable Genome-Associated LncRNAs (DrugGenoLnc) and conducted functional enrichment analysis, revealing their roles in LUAD biology. Furthermore, we conducted Mendelian randomization (MR) and Bayesian weighted Mendelian Randomization (BWMR) analysis using TwoSampleMR to explore possible lung cancer-related pathways. Our assessment of the tumor microenvironment included tumor mutational burden (TMB), the TIDE algorithm, and the “pRRophetic” R package. Additionally, we analyzed stemness indices in LUAD patients.</div></div><div><h3>Results</h3><div>Our lncRNA-centered prognostic model demonstrated significant value for risk stratification. Functional enrichment analysis highlighted lncRNAs’ involvement in vital biological processes. MR and BWMR analysis confirmed the inhibitory effect of the “neutrophil extracellular trap formation” pathway on NSCLC. Immunological analysis identified high-risk pathways related to immune functions, potentially enhancing immunotherapy prospects for high-risk patients. Patients with high TMB had poorer overall survival, while high-risk patients showed increased chemotherapy drug sensitivity. Lastly, mRNA stem cell index (mRNAsi) correlated with LUAD patient prognosis.</div></div><div><h3>Conclusion</h3><div>This study underscores DrugGenoLnc’s utility as a prognostic feature, establishes a robust prognostic model for LUAD, and offers potential for early detection markers and therapeutic target identification. Furthermore, it provides insights into the anti-tumor immune microenvironment, guiding clinical treatment strategies in LUAD.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"16 ","pages":"Article 100053"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of probiotics in mercury detoxification: a systematic review","authors":"Moulik Bhattacharyya ,&nbsp;Serina Easmin ,&nbsp;Dipjyoti Sharma ,&nbsp;Pollobi Porasar ,&nbsp;Tanusree Karmakar ,&nbsp;Koushik Nandan Dutta ,&nbsp;Ranabir Sahu ,&nbsp;Gouranga Nandi ,&nbsp;Paramita Paul ,&nbsp;Tarun Kumar Dua","doi":"10.1016/j.meomic.2026.100054","DOIUrl":"10.1016/j.meomic.2026.100054","url":null,"abstract":"<div><div>Mercury is a very toxic heavy metal that inflicts neurological, reproductive, and metabolic impairment, yet current chelation therapies are constrained in both safety and efficacy. This study assesses the effectiveness of probiotics as a biotherapeutic strategy for mercury detoxification. Probiotics protect by physically sequestering mercury through cell wall components, enhancing antioxidant defenses via the Nrf2/Keap1-ARE signaling pathway, modulating inflammatory responses by downregulating the NF-κB and MAPK pathways, and maintaining intestinal barrier integrity. Translational findings suggest that probiotic therapies may function as economical and culturally acceptable dietary options for mitigating mercury exposure, especially in populations vulnerable due to contaminated food or environmental factors. Research from in vitro models, animal studies, and initial human trials suggests that strains such as <em>Lactobacillus</em>, <em>Bifidobacterium</em>, and <em>Bacillus</em> can bind to and sequester mercury in the gastrointestinal tract, enhance its fecal excretion, convert it into less toxic forms, and restore antioxidant, immune, and intestinal functions. Despite initial encouraging outcomes, extensive clinical studies and the selection of specific strains are necessary to substantiate probiotics as a viable therapeutic strategy for mercury detoxification. This study suggests that probiotics are a safe, sustainable, and promising approach to mitigating mercury toxicity; however, additional clinical validation and strain-specific modifications are necessary.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"16 ","pages":"Article 100054"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of a novel multi-epitope-based vaccine against Bundibugyo Ebolavirus using computational approach","authors":"Salman Khan ,&nbsp;Ishaq Khan ,&nbsp;Arshad Iqbal ,&nbsp;Syed Shujait Ali ,&nbsp;Nisar Ahmad ,&nbsp;Sami Ullah ,&nbsp;Liaqat Ali ,&nbsp;Sajjad Ahmad","doi":"10.1016/j.meomic.2025.100050","DOIUrl":"10.1016/j.meomic.2025.100050","url":null,"abstract":"<div><h3>Background</h3><div>Ebola virus (EBOV), a member of the Filoviridae family, is a highly lethal disease that has caused multiple outbreaks primarily in Central Africa. According to the World Health Organization (WHO) report, the Ebola Virus Disease death rate in 2019 was around 67 %. Specifically, Bundibugyo Ebolavirus (BEBOV) has a relatively lower pathogenic mortality rate, estimated at 37 %. Previous epidemics have seen varying death rates ranging from 25 to 95 % due to the lack of effective medicines and immunizations.</div></div><div><h3>Objectives</h3><div>This study aimed to design a multi-epitope vaccine against BEBOV using immunoinformatics to induce robust immune responses.</div></div><div><h3>Methods and results</h3><div>T-cell (CTL and HTL) and linear B-cell epitopes from spike glycoprotein and nucleoprotein were identified and screened for antigenicity (VaxiJen score: 0.6488), allergenicity, and immunogenicity. Selected epitopes were combined into a vaccine construct with beta-defensin as an adjuvant. The vaccine exhibited favorable physicochemical properties and solubility (0.863482). Molecular docking and dynamics simulations revealed strong binding to TLR-3 (docking score: −354.13 kcal/mol). Codon optimization and in silico cloning suggested efficient expression in Escherichia coli.</div></div><div><h3>Conclusion and future recommendation</h3><div>The designed multi-epitope vaccine demonstrates promising immunogenic potential against BEBOV. Experimental validation is required to confirm efficacy and safety, and further studies should focus on optimization and clinical evaluation for potential outbreak prevention.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"16 ","pages":"Article 100050"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145750170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of secretome from areca nut-exposed fibroblast cells on malignant transformation of oral epithelial cells","authors":"Tarun Prakash Verma ,&nbsp;Sonali Adhikari ,&nbsp;Siddharth Singh ,&nbsp;Ajay Kumar Meena ,&nbsp;Hem Chandra Jha","doi":"10.1016/j.meomic.2026.100055","DOIUrl":"10.1016/j.meomic.2026.100055","url":null,"abstract":"<div><div>The intercellular signaling between fibroblast cells and epithelial keratinocytes in oral cavity plays an important role in the malignant transformation of oral submucous fibrosis (OSMF) to oral squamous cell carcinoma (OSCC). Hence, the current study was designed to evaluate the effect of the secretome from the areca nut-exposed fibroblast cells on the oral keratinocytes. We collected the conditioned media (CM) from the fibroblast cells exposed with the areca nut extract (10 µg/ml) for 48 h and transferred it to the keratinocyte cells, followed by incubation for 48 h. We then assessed the cell proliferation, mRNA expression for OSCC-related genes, and metabolomics of the CM and cell lysate by liquid chromatography-mass spectrometry (LCMS). We found a higher proliferation in the cells incubated with exposed CM compared to unexposed CM. The mRNA expression profiles show significant upregulation of five genes (<em>pcsk9, mmp12, inhba, mmp11</em> and <em>hoxd11</em>). The metabolomics shows alteration of tryptophan metabolism, pyrimidine metabolism, aspartate &amp; asparagine metabolism in CM, and glycolysis and gluconeogenesis in the cell lysate. In conclusion, we identified the genes and pathways that are affected by the secretome from areca nut-exposed fibroblast cells and may have a role in the malignant transformation of oral submucous fibrosis.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"16 ","pages":"Article 100055"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical analysis, evaluation of antioxidant activity, and investigation of antibacterial activity in euphorbia candelabrum root extracts","authors":"Kifle Jilo Jego ,&nbsp;Legesse Adane ,&nbsp;Fillipo Tamiru","doi":"10.1016/j.meomic.2025.100051","DOIUrl":"10.1016/j.meomic.2025.100051","url":null,"abstract":"<div><div>Euphorbia candelabrum is a medicinal plant traditionally used for treating coughs, tuberculosis, malaria, wound healing, and various skin conditions. This study aimed to analyze the secondary metabolites present in its root extract and evaluate its antioxidant and antibacterial properties. Samples were collected from Sika Kebele in the Gedeo Zone of Southern Ethiopia, air-dried, and then extracted using hexane, dichloromethane, a methanol:dichloromethane mixture (1:1 v/v), and methanol. Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolics, glycosides, terpenoids, tannins, and saponins in the dichloromethane, methanol:dichloromethane 1:1 mixture, and methanol extracts. In contrast, the hexane extract contained only alkaloids, terpenoids, and steroids. Notably, the methanol:dichloromethane extract yielded flavonoids (10.47 mg QE/g), phenolics (13.4 mg GAE/g), alkaloids (1.426 mg AE/g), and steroids (2.91 mg DME/g). The methanol extract demonstrated higher levels of flavonoids (11 mg QE/g) and phenolics (19 mg GAE/g). Antibacterial activity was assessed against E. coli, P. aeruginosa, S. aureus, and S. pyogenes using the disc diffusion method. The isolated compounds exhibited greater antibacterial activity than crude extracts, with compound KJ-3 showing greater activity against all tested strains, slightly less than tetracycline. These findings suggest that the root of Euphorbia candelabrum may serve as a promising source for the development of new antibacterial agents.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"16 ","pages":"Article 100051"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145694764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mezoneuron benthamianum attenuates indomethacin-induced toxicity: Design, synthesis, elemental analysis, in vivo and in silico studies","authors":"Oluwasayo P. Abodunrin ,&nbsp;Olayinka F. Onifade ,&nbsp;P.M. Osamudiamen ,&nbsp;Zacchaeus S. Ololade ,&nbsp;Esther O. Abam ,&nbsp;Somto O. Otega ,&nbsp;Faith O. James ,&nbsp;Benjamen O. Okunlola","doi":"10.1016/j.meomic.2025.100048","DOIUrl":"10.1016/j.meomic.2025.100048","url":null,"abstract":"<div><h3>Introduction</h3><div>Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for pain relief and anti-inflammatory effects but can cause significant liver damage and oxidative stress. <em>Mezoneuron benthamianum,</em> a plant with medicinal properties, has been traditionally used for various treatments.</div></div><div><h3>Methods</h3><div>This study evaluates the hepatoprotective and antioxidant effects of <em>Mezoneuron benthamianum</em> leaves extract against NSAID-induced toxicity in albino rats. Rats were divided into five groups: control, indomethacin-treated, <em>Mezoneuron benthamianum</em> extract-treated, indomethacin and <em>Mezoneuron benthamianum</em> co-treated, and indomethacin and methyl gallate co-treated. The extract’s biochemical composition was analyzed using GC–MS, and liver function biomarkers (ALT, AST, ALP) and biochemical markers (MDA, SOD, CAT, GSH) were measured.</div></div><div><h3>Results and discussion</h3><div>Results showed that the indomethacin-treated group had significantly increased ALT, AST, and ALP levels, elevated MDA levels, and reduced SOD, CAT, and GSH activities, indicating liver damage and oxidative stress. Treatment with <em>Mezoneuron benthamianum</em> extract significantly reduced these biomarkers and restored antioxidant enzyme activities. Histopathological analysis confirmed reduced liver damage in the extract-treated group, with similar protective effects observed in the indomethacin and methyl gallate co-treated group. The concentration of Cd marginally exceeded WHO’s limit (0.3412 mg/kg vs. 0.3 mg/kg) whereas concentrations of Fe, Mn, and Cu were well below the thresholds. The hepatoprotective and antioxidant effects of <em>Mezoneuron benthamianum</em> extract might be partially mediated through interactions with essential trace elements, such as Fe, Cu, and Mn.</div></div><div><h3>Conclusion</h3><div>The study reveals that <em>Mezoneuron benthamianum</em> extract attenuated liver damage by restoring antioxidant enzymes (SOD, CAT, GSH) and reducing oxidative stress markers (MDA). Molecular docking suggested PPAR-α activation as a potential mechanism, though further validation is needed. The study highlights its hepatoprotective potential but warrants dose-response studies for clinical translation.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"15 ","pages":"Article 100048"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145580411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a prognostic signature for bladder cancer associated with the epithelial-mesenchymal transition hallmark pathway using the TCGA Database","authors":"Shabiha Afroj Heeamoni,&nbsp;Safayat Mahmud Khan,&nbsp;Md. Omar Faruk,&nbsp;Md. Ismail Hosen","doi":"10.1016/j.meomic.2025.100049","DOIUrl":"10.1016/j.meomic.2025.100049","url":null,"abstract":"<div><div>Bladder cancer is a common urological malignancy with poor prognosis, underscoring the need for robust prognostic biomarkers. This study investigated gene expression profiles of 194 epithelial-mesenchymal transition (EMT) hallmark genes in 411 muscle-invasive bladder cancer patients from The Cancer Genome Atlas (TCGA). Non-negative matrix factorization identified two subtypes (C1 and C2) with distinct prognostic, clinical, and immunological characteristics. C2 was enriched in EMT-related pathways, including extracellular matrix and structure organization. Differential expression analysis revealed 1,514 prognosis-associated genes. A four-gene prognostic signature—<strong>SIGLEC6, NEFL, NTNG1,</strong> <!-->and<!--> <strong>FOXC2</strong>—was constructed using univariate, LASSO, and multivariate Cox regression models. Patients were stratified into high- and low-risk groups, with the signature demonstrating predictive value in survival analysis and ROC curves. External validation was performed using GEO data. This EMT-based four-gene signature offers a promising prognostic tool for bladder cancer patient stratification and potential therapeutic targeting.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"15 ","pages":"Article 100049"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145529487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a highly antigenic multi epitope subunit vaccine against Bovine alpha herpes virus 2 targeting glycoprotein B and H: A reverse vaccinology approach","authors":"Ali Khan,&nbsp;Sara Khan,&nbsp;Muhammad Rahiyab,&nbsp;Salman Khan,&nbsp;Zabih Ullah,&nbsp;Syed Shujait Ali","doi":"10.1016/j.meomic.2025.100047","DOIUrl":"10.1016/j.meomic.2025.100047","url":null,"abstract":"<div><div>Bovine alphaherpesvirus 2 (BoHV-2), a major pathogen in cattle, belongs to the <em>Herpesviridae</em> family. In this work, we designed a vaccine against BoHV-2 using its envelope glycoprotein B (gB) and glycoprotein H. In this work, computational methods were utilized for predicting the B and T lymphocyte epitopes with striking results in their antigenicity and low allergenicity. Thereafter, the vaccine design stability tests showed that it was physicochemically stable. Molecular docking and molecular dynamics simulation analyses validated its further efficacy. The docking results demonstrated 11 hydrogen bonds coupled with one salt bridge between the vaccine and TLR3 while PRODIGY analysis suggested a binding free energy (ΔG) of −10.7 kcal/mol alongside a dissociation constant (kDa) of 1.5e-08. In silico cloning demonstrations showed effective expression levels together with immune simulation predictions, suggesting robust immunological responses. However, this study has certain limitations, such as the experimental validation. Future <em>in vivo</em> and <em>in vitro</em> studies are required to confirm the immunogenicity and safety of the in-silico designed vaccine construct.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"14 ","pages":"Article 100047"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics integration reveals chromatin-associated lncRNA prognostic model in lung adenocarcinoma: Bridging GWAS, transcriptome and clinical outcomes","authors":"Xiao Zhu ,&nbsp;Zhuolong Xiong","doi":"10.1016/j.meomic.2025.100045","DOIUrl":"10.1016/j.meomic.2025.100045","url":null,"abstract":"<div><h3>Background</h3><div>Lung adenocarcinoma (LUAD) is characterized by low overall survival rates. This research aims to explore the association between long non-coding RNAs (lncRNAs) and chromatin histone methylation/demethylation modifiers in LUAD.</div></div><div><h3>Methods</h3><div>Datasets from The Cancer Genome Atlas (TCGA), Molecular Signatures Database (MSigDB), and IEU Open genome-wide association studies (GWAS) database were analyzed. A prognostic risk model for LUAD was developed based on 32 lncRNAs linked to histone modification. The relationship between lncRNAs and the high-risk group of lung cancer was evaluated, and GO/KEGG analysis was conducted to investigate the connection between chromatin histone modification-related lncRNAs and biological processes/pathways. Mendelian Randomization methods, including Inverse Variance Weighted (IVW) and Bayesian Weighted Mendelian Randomization (BWMR), were employed to validate the GO/KEGG results. MR-Egger intercept test, Cochran’s Q test, and leave-one-out Analysis were utilized to assess the sensitivity of Mendelian Randomization analysis. Tumor mutation burden (TMB) analysis was performed to evaluate the prognostic impact of high-risk patients with high TMB.</div></div><div><h3>Results</h3><div>Identified lncRNAs, including AC025741.1 and NHS-AS1, demonstrated strong associations with the high-risk group. GO/KEGG analysis revealed significant correlations between chromatin histone modification-related lncRNAs and microtubule-based movement and cytochrome enzyme P450. Response to the renin-angiotensin agents is a protective factor for lung cancer, while response to glucocorticoids is a risk factor for lung cancer. Immunomarkers MDSC, CAF, and Exclusion showed positive correlations with the risk score, and the combined effects of CAF and MDSC were found to play a pivotal role in LUAD development and progression.</div></div><div><h3>Conclusion</h3><div>Our study not only establishes a promising LUAD prognostic risk model with potential implications for immunotherapy but also identifies lncRNAs as immune markers for LUAD immunotherapy. Additionally, we validate the causal relationship between chromatin histone methylation-related pathways and lung cancer, bolstering our understanding from a genetic perspective and opening avenues for targeted interventions in LUAD treatment.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"14 ","pages":"Article 100045"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of zebrafish homologs within the human genome provides valuable insights into exploring pan-cancer alternative splicing events as prognostic markers and therapeutic targets","authors":"Yanjun Liang ,&nbsp;Xiao Zhu","doi":"10.1016/j.meomic.2025.100046","DOIUrl":"10.1016/j.meomic.2025.100046","url":null,"abstract":"<div><div>Alternative splicing (AS) is pivotal in gene expression regulation and protein diversity generation. Suppression of aberrant AS holds promise in cancer management. Utilizing zebrafish-human homologous genes, we investigated AS events across 33 tumor types from TCGA, employing Cox regression analyses to identify prognostic AS events. A risk-scoring model distinguished high and low-risk groups, revealing differences in survival, tumor microenvironment, and immune cell infiltration. Notably, Complement C1q C Chain (C1QC), DENND5A, HP, IRF5, and LRP1 were associated with poor prognosis and immune infiltration. Mendelian randomization confirmed the protective role of C1QC across cancers, further supported by Bayesian Weighted Mendelian Randomization. Leveraging zebrafish homologs enhances our understanding of AS mechanisms and potential therapeutic targets in cancer biology.</div></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"14 ","pages":"Article 100046"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}