Methods explored in order to make molecular imprints with phenylalanine ethyl esters in styrene and acrylic polymers using a non-covalent imprinting technique are described. Amino acid ester-selective polymers were prepared after ion-pair association of substrate (print molecule) and carboxyl-containing monomers in the polymerization step. A high concentration of cross-linking agents (divinylbenzene or ethylene glycol dimethacrylate) was present in the polymerization mixtures in order to produce macroporous polymers of high rigidity. After polymerization, polymers were washed free from print molecules and the substrate-selective polymers formed were examined by incubation of the polymers with racemic mixtures of doubly radiolabelled phenylalanine ethyl ester. Binding experiments in batch and chromatographic procedures have been performed. It was found that separation factors determined for polymers prepared in the presence of d- or l-phenylalanine ethyl ester were different from one, indicating that the polymers formed were chiral. It was also found that the polymers interacted preferentially with the enantiomer of the print molecule present in the polymerization mixture. Finally, a synthetic procedure has been worked out for making new amino acid-based cross-linkers suitable for preparation of substrate-selective acrylic polymers. The method utilizes modification of aminoalcohols by acryloyl chloride substitution.
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