首页 > 最新文献

Total Environment Research Themes最新文献

英文 中文
Physicochemical and microbiological characterization and of hospital wastewater in Tanzania 坦桑尼亚医院废水的理化和微生物特性
Pub Date : 2023-08-25 DOI: 10.1016/j.totert.2023.100075
Petro Karungamye , Anita Rugaika , Kelvin Mtei , Revocatus Machunda

Given the complex composition of hospital wastewater and the high risk of initiating disease outbreaks, comprehensive monitoring and treatment of hospital wastewater are required to prevent social and environmental consequences. This study investigated the physicochemical and microbiological characteristics of wastewater from the Benjamin Mkapa Hospital in Dodoma Tanzania. The wastewater from this hospital is treated in a horizontal flow Constructed Wetland (CW) planted with Typha latifolia before being discharged into the environments. Wastewater samples were collected at the CW inlet and outlet from 02nd May 2022 to 25th July 2022. The results shows that the effluent discharged had pH 7.48 ± 0.63, electrical conductivity 2441 ± 623 µS/cm, Total dissolved solids 1305.5 ± 396 mg/L, Total suspended solids 49.17 ± 53.11 mg/L, Turbidity 9.1 ± 14.83 NTU, COD 170.4 ± 40.6 mg/L, BOD5 74.8 ± 33.5 mg/L, NO3-N 45.4 ± 39.97 mg/L and PO4-P 4.52 ± 2.30 mg/L. The CW removed TSS by 82% and turbidity 94%. COD, BOD and NO3-N were removed by 48%, 47% and 58% respectively. E. coli concentration in effluent samples ranged from 1.1 × 101 CFU/mL to 1.1 × 102 CFU/mL with an average of 1.77logCFU/mL. Average BOD5/COD ratio was 0.5 and 0.4 for influent and effluent respectively. The effluent contained higher levels of EC, TDS, and PO4-P than the influent. According to the findings of this study, most of the parameters of wastewater effluent discharged wasn't within the effluent discharge standards.

鉴于医院废水的成分复杂,引发疾病爆发的风险很高,需要对医院废水进行全面监测和处理,以防止社会和环境后果。本研究调查了坦桑尼亚多多马Benjamin Mkapa医院废水的理化和微生物特性。该医院的废水在排放到环境中之前,在种植有宽叶香蒲的水平流人工湿地(CW)中进行处理。从2022年5月2日至2022年7月25日,在CW入口和出口收集废水样本。结果表明,出水pH值7.48±0.63,电导率2441±623µS/cm,总溶解固体1305.5±396 mg/L,总悬浮固体49.17±53.11 mg/L,浊度9.1±14.83 NTU,COD 170.4±40.6 mg/L,BOD5 74.8±33.5 mg/L,NO3-N 45.4±39.97 mg/L,PO4-P 4.52±2.30 mg/L。CW去除TSS 82%,浊度94%。COD、BOD和NO3-N的去除率分别为48%、47%和58%。出水样品中大肠杆菌浓度范围为1.1×101CFU/mL至1.1×102CFU/mL,平均1.77logCFU/mL。进水和出水的BOD5/COD平均比值分别为0.5和0.4。出水的EC、TDS和PO4-P含量高于进水。根据本研究的结果,污水排放的大部分参数都不在污水排放标准范围内。
{"title":"Physicochemical and microbiological characterization and of hospital wastewater in Tanzania","authors":"Petro Karungamye ,&nbsp;Anita Rugaika ,&nbsp;Kelvin Mtei ,&nbsp;Revocatus Machunda","doi":"10.1016/j.totert.2023.100075","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100075","url":null,"abstract":"<div><p>Given the complex composition of hospital wastewater and the high risk of initiating disease outbreaks, comprehensive monitoring and treatment of hospital wastewater are required to prevent social and environmental consequences. This study investigated the physicochemical and microbiological characteristics of wastewater from the Benjamin Mkapa Hospital in Dodoma Tanzania. The wastewater from this hospital is treated in a horizontal flow Constructed Wetland (CW) planted with <em>Typha latifolia</em> before being discharged into the environments. Wastewater samples were collected at the CW inlet and outlet from 02nd May 2022 to 25th July 2022. The results shows that the effluent discharged had pH 7.48 ± 0.63, electrical conductivity 2441 ± 623 µS/cm, Total dissolved solids 1305.5 ± 396 mg/L, Total suspended solids 49.17 ± 53.11 mg/L, Turbidity 9.1 ± 14.83 NTU, COD 170.4 ± 40.6 mg/L, BOD<sub>5</sub> 74.8 ± 33.5 mg/L, NO<sub>3</sub>-N 45.4 ± 39.97 mg/L and PO<sub>4</sub>-P 4.52 ± 2.30 mg/L. The CW removed TSS by 82% and turbidity 94%. COD, BOD and NO<sub>3</sub>-N were removed by 48%, 47% and 58% respectively. <em>E. coli</em> concentration in effluent samples ranged from 1.1 × 10<sup>1</sup> CFU/mL to 1.1 × 10<sup>2</sup> CFU/mL with an average of 1.77logCFU/mL. Average BOD<sub>5</sub>/COD ratio was 0.5 and 0.4 for influent and effluent respectively. The effluent contained higher levels of EC, TDS, and PO<sub>4</sub>-P than the influent. According to the findings of this study, most of the parameters of wastewater effluent discharged wasn't within the effluent discharge standards.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"8 ","pages":"Article 100075"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49730451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enterobacter species Distribution, emerging virulence and multiple antibiotic resistance dynamics in effluents: A countrified spread-hub and implications of abattior release 肠杆菌种类分布、新出现的毒力和污水中多种抗生素耐药性动态:一个国家传播中心和屠宰释放的影响
Pub Date : 2023-08-14 DOI: 10.1016/j.totert.2023.100074
Rashidat B. Maikalu , Bright E. Igere , Emmanuel E.O. Odjadjare

Abattoirs operations are adjudged as social service that impact the business development/financial status of societies however, it’s release have been implicated as emerging environmental contaminant. Coincidentially, reports on the spread of multiple antibiotics resistant (MAR) potential enterobacteriaceae from abattoir release continues to undulate with some ambiguity. This study determines the Enterobacter species distribution, emerging virulence and multiple antibiotic resistance dynamics in effluents: a countrified spread-hub and implications of abattior release. Using both standard microbiological/molecular biology techniques, abattoir effluent released into the receiving river at Ikpoba-Oha community, Benin City, Edo State, Nigeria was collected at catchment points. A six-weekly grab (once-off) sample was collected between February and April 2019 from Point Source (PS), Confluent Point (CP), 500 m Upstream (UP) and 500 m Downstream (DS) of CP for microbiological and molecular biology processing. Our result revealed that the bacterial density and total heterotrophic count in PS were significantly (p < 0.01) higher than those recorded in UP and DS. The morphological, biochemical and phenotypic characteristics of the presumptive isolates reveals eight Enterobacter spp. with phenotypic virulence determinants including β- heamolysis, gelatinase production, biofilm formation etc amongst isolates. The 16S rRNA gene PCR detection and partial gene sequencing confirmed two strains of Enterobacter cloacae which are deposited as Enterobacter cloacae BIU RASH1 and Enterobacter cloacae BIU RASH7 with genebank ascension numbers of MN577420 and MN581680. These strains antibiotic susceptibility testing reports showed MAR phenotypes on antibiotics ranging from fluoroquinolone, β-lactam, cephalosporin, tetracycline etc, and MAR-index of 0.82. Such observations amongst abattoir effluents isolates implicates abattoir as potential pathogen distribution hub if adequate control steps are not instituted in affected communities. There is therefore need to ensure that wastes from abattoirs are adequately treated to eliminate potential pathogens before discharge into the environmental water resources to prevent potential public health concerns associated with such release.

屠宰场的经营被判定为影响社会商业发展/金融状况的社会服务,然而,它的释放被认为是新出现的环境污染物。巧合的是,关于屠宰场释放的多种抗生素耐药性(MAR)潜在肠杆菌科传播的报道仍在波动,但有些模糊。这项研究确定了肠杆菌的物种分布、新出现的毒力和废水中的多种抗生素耐药性动态:一个乡村传播中心和杀菌剂释放的影响。使用标准微生物/分子生物学技术,在尼日利亚埃多州贝宁市Ikpoba Oha社区的集水区收集排入接收河的屠宰场污水。在2019年2月至4月期间,从CP的点源(PS)、汇合点(CP)、上游500米(UP)和下游500米(DS)采集了六周一次的取样(一次性),用于微生物和分子生物学处理。我们的结果显示,PS中的细菌密度和总异养计数显著(p<;0.01)高于UP和DS中的记录。推定分离株的形态、生化和表型特征揭示了8种肠杆菌属。其表型毒力决定因素包括β-溶血、明胶酶产生、,分离物之间的生物膜形成等。16S rRNA基因PCR检测和部分基因测序证实了两株阴沟肠杆菌,分别为阴沟肠菌BIU RASH1和阴沟肠细菌BIU RASH7,基因库提升数分别为MN577420和MN581680。这些菌株的抗生素敏感性测试报告显示,对氟喹诺酮、β-内酰胺、头孢菌素、四环素等抗生素的MAR表型,MAR指数为0.82。如果在受影响的社区没有采取足够的控制措施,屠宰场废水分离株中的这些观察结果表明,屠宰场是潜在的病原体分布中心。因此,有必要确保屠宰场的废物在排入环境水资源之前得到充分处理,以消除潜在的病原体,以防止与此类排放相关的潜在公共卫生问题。
{"title":"Enterobacter species Distribution, emerging virulence and multiple antibiotic resistance dynamics in effluents: A countrified spread-hub and implications of abattior release","authors":"Rashidat B. Maikalu ,&nbsp;Bright E. Igere ,&nbsp;Emmanuel E.O. Odjadjare","doi":"10.1016/j.totert.2023.100074","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100074","url":null,"abstract":"<div><p>Abattoirs operations are adjudged as social service that impact the business development/financial status of societies however, it’s release have been implicated as emerging environmental contaminant. Coincidentially, reports on the spread of multiple antibiotics resistant (MAR) potential enterobacteriaceae from abattoir release continues to undulate with some ambiguity. This study determines the Enterobacter species distribution, emerging virulence and multiple antibiotic resistance dynamics in effluents: a countrified spread-hub and implications of abattior release. Using both standard microbiological/molecular biology techniques, abattoir effluent released into the receiving river at Ikpoba-Oha community, Benin City, Edo State, Nigeria was collected at catchment points. A six-weekly grab (once-off) sample was collected between February and April 2019 from Point Source (PS), Confluent Point (CP), 500 m Upstream (UP) and 500 m Downstream (DS) of CP for microbiological and molecular biology processing. Our result revealed that the bacterial density and total heterotrophic count in PS were significantly (p &lt; 0.01) higher than those recorded in UP and DS. The morphological, biochemical and phenotypic characteristics of the presumptive isolates reveals eight <em>Enterobacter</em> spp. with phenotypic virulence determinants including β- heamolysis, gelatinase production, biofilm formation etc amongst isolates. The 16S rRNA gene PCR detection and partial gene sequencing confirmed two strains of <em>Enterobacter cloacae</em> which are deposited as <em>Enterobacter cloacae</em> BIU RASH1 and <em>Enterobacter cloacae</em> BIU RASH7 with genebank ascension numbers of MN577420 and MN581680. These strains antibiotic susceptibility testing reports showed MAR phenotypes on antibiotics ranging from fluoroquinolone, β-lactam, cephalosporin, tetracycline etc, and MAR-index of 0.82. Such observations amongst abattoir effluents isolates implicates abattoir as potential pathogen distribution hub if adequate control steps are not instituted in affected communities. There is therefore need to ensure that wastes from abattoirs are adequately treated to eliminate potential pathogens before discharge into the environmental water resources to prevent potential public health concerns associated with such release.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"8 ","pages":"Article 100074"},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49730701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of stress tolerance index for screening of multiple abiotic stress tolerant genotype and determination of reliable trait in Cicer arieitnum L. 茜草耐逆指数的优化筛选及可靠性状的确定。
Pub Date : 2023-08-11 DOI: 10.1016/j.totert.2023.100073
Ravneet kaur , Harleen kaur , Swapnil Singh , Neetu Jagota , Shayla Bindra , Ashish Sharma

One of the major problems affecting agricultural productivity is high soil salt content that causes increased reactive oxygen species production affecting membrane stability and cell metabolism. Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous persistent hydrocarbons comprising two or more fused benzene rings that severely affect plant growth. The study was carried out to screen genotypes of chickpea (Cicer arietinum L.) based on responses to the presence of different concentrations of NaCl, Phenanthrene, and a combination of both NaCl and Phenanthrene, which is the first report of chickpea growth under combination of NaCl and Phenanthrene. Four Chickpea genotypes were grown under three concentrations of NaCl and Phenanthrene respectively and combinations of both NaCl and Phenanthrene. A 21 day in-vitro experiment comprised of growing chickpea in petriplates and growth pouches. Morphological parameters were recorded to differentiate susceptible and tolerant genotypes based on the Stress Tolerance Index. MFV was calculated to further evaluate genotypes under different stress conditions. Results revealed that GPF2 and PBG7 have highest and least STI values respectively hence, GPF2 and PBG7 was tolerant and sensitive genotypes respectively towards the individual and combined effect of NaCl and Phenenathrene. Linear regression analysis revealed that root length can be the reliable trait to study the effect of salt stress on chickpea genotypes while GI can be used as reliable trait to study the effect of Phenanthrene stress and combined stress on chickpea genotypes.

影响农业生产力的主要问题之一是土壤含盐量高,导致活性氧产生增加,影响膜稳定性和细胞代谢。多环芳烃(PAHs)是一种普遍存在的持久性碳氢化合物,包含两个或多个稠苯环,严重影响植物生长。本研究基于对不同浓度的NaCl、菲以及NaCl和菲的组合的反应来筛选鹰嘴豆(Cicer arietinum L.)的基因型,这是首次报道了鹰嘴豆在NaCl和菲组合下的生长。四种鹰嘴豆基因型分别在三种浓度的NaCl和菲以及NaCl和菲的组合下生长。A 21 一天的体外实验包括在鹰嘴豆板和生长袋中种植鹰嘴豆。根据应激耐受指数记录形态学参数以区分易感和耐受基因型。计算MFV以进一步评估不同胁迫条件下的基因型。结果表明,GPF2和PBG7分别具有最高和最低的STI值,因此GPF2和PBG7分别对NaCl和Pheneathrene的单独作用和联合作用具有耐受性和敏感性。线性回归分析表明,根长可以作为研究盐胁迫对鹰嘴豆基因型影响的可靠性状,而GI可以作为研究菲胁迫和复合胁迫对鹰嘴豆种型影响的可靠性性状。
{"title":"Optimization of stress tolerance index for screening of multiple abiotic stress tolerant genotype and determination of reliable trait in Cicer arieitnum L.","authors":"Ravneet kaur ,&nbsp;Harleen kaur ,&nbsp;Swapnil Singh ,&nbsp;Neetu Jagota ,&nbsp;Shayla Bindra ,&nbsp;Ashish Sharma","doi":"10.1016/j.totert.2023.100073","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100073","url":null,"abstract":"<div><p>One of the major problems affecting agricultural productivity is high soil salt content that causes increased reactive oxygen species production affecting membrane stability and cell metabolism. Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous persistent hydrocarbons comprising two or more fused benzene rings that severely affect plant growth. The study was carried out to screen genotypes of chickpea (<em>Cicer arietinum</em> L.) based on responses to the presence of different concentrations of NaCl, Phenanthrene, and a combination of both NaCl and Phenanthrene, which is the first report of chickpea growth under combination of NaCl and Phenanthrene. Four Chickpea genotypes were grown under three concentrations of NaCl and Phenanthrene respectively and combinations of both NaCl and Phenanthrene. A 21 day <em>in-vitro</em> experiment comprised of growing chickpea in petriplates and growth pouches. Morphological parameters were recorded to differentiate susceptible and tolerant genotypes based on the Stress Tolerance Index. MFV was calculated to further evaluate genotypes under different stress conditions. Results revealed that GPF2 and PBG7 have highest and least STI values respectively hence, GPF2 and PBG7 was tolerant and sensitive genotypes respectively towards the individual and combined effect of NaCl and Phenenathrene. Linear regression analysis revealed that root length can be the reliable trait to study the effect of salt stress on chickpea genotypes while GI can be used as reliable trait to study the effect of Phenanthrene stress and combined stress on chickpea genotypes.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"8 ","pages":"Article 100073"},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49730557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soil erosion and sediment yield modeling in a tropical mountain watershed of the southern Western Ghats, India using RUSLE and Geospatial tools 利用RUSLE和地理空间工具在印度西高止山脉南部热带山地流域进行土壤侵蚀和产沙模拟
Pub Date : 2023-08-11 DOI: 10.1016/j.totert.2023.100072
A.L. Achu , Jobin Thomas

The development of comprehensive soil conservation strategies, based on the soil erosion risk, is one of the critical components of sustainable river basin management, especially in tropical mountainous regions. The present study computes the spatially distributed longtime average annual soil loss (A) and sediment yield (SY) and prioritizes the soil erosion risk in a tropical mountain river basin. The Revised Universal Soil Loss Equation (RUSLE) and the sediment delivery distributed model (SEDD) models were coupled in the GIS environment to predict the intra-basin variability of the A and SY of the Karimpuzha River Basin (KRB) of the southern Western Ghats (in Kerala, India). The results indicate that, on an average basis, 9.3 t of soil has been eroded per hectare per year, whereas the SY is 6.3 t ha−1 y−1 (i.e., approximately two-thirds of the gross soil erosion). The SY of the majority (74.2%) of the basin area is low (i.e., ≤5.0 t ha−1 y−1), whereas the soil erosion and transport processes are a major concern in some areas (ca. 6.3 km2). The results imply the significance of land use/land cover, rainfall, and topography in controlling the soil erosion risk of the basin. The study demonstrates the advantages of the delineation of the critical erosion areas, based on the hydrologic response units (HRUs) of the basin, to implement appropriate soil erosion management options for conservation of the soil resources.

基于土壤侵蚀风险制定综合土壤保持战略是流域可持续管理的关键组成部分之一,尤其是在热带山区。本研究计算了热带山区河流流域长期平均年土壤流失量(A)和产沙量(SY)的空间分布,并对土壤侵蚀风险进行了优先排序。在GIS环境中,将修订的通用土壤流失方程(RUSLE)和输沙分布模型(SEDD)模型相结合,以预测西高止山脉南部(印度喀拉拉邦)Karimpuzha河流域(KRB)A和SY的流域内变化。结果表明,平均每年每公顷有9.3 t土壤受到侵蚀,而SY为6.3 t ha−1 y−1(即约占总土壤侵蚀的三分之二)。流域大部分地区(74.2%)的SY较低(即≤5.0 t ha−1 y−1),而土壤侵蚀和迁移过程是一些地区(约6.3 km2)的主要问题。研究结果表明,土地利用/土地覆盖、降雨和地形在控制流域土壤侵蚀风险方面具有重要意义。该研究证明了基于流域水文响应单元(HRU)划定关键侵蚀区的优势,以实施适当的土壤侵蚀管理方案来保护土壤资源。
{"title":"Soil erosion and sediment yield modeling in a tropical mountain watershed of the southern Western Ghats, India using RUSLE and Geospatial tools","authors":"A.L. Achu ,&nbsp;Jobin Thomas","doi":"10.1016/j.totert.2023.100072","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100072","url":null,"abstract":"<div><p>The development of comprehensive soil conservation strategies, based on the soil erosion risk, is one of the critical components of sustainable river basin management, especially in tropical mountainous regions. The present study computes the spatially distributed longtime average annual soil loss (A) and sediment yield (SY) and prioritizes the soil erosion risk in a tropical mountain river basin. The Revised Universal Soil Loss Equation (RUSLE) and the sediment delivery distributed model (SEDD) models were coupled in the GIS environment to predict the intra-basin variability of the A and SY of the Karimpuzha River Basin (KRB) of the southern Western Ghats (in Kerala, India). The results indicate that, on an average basis, 9.3 t of soil has been eroded per hectare per year, whereas the SY is 6.3 t ha<sup>−1</sup> y<sup>−1</sup> (i.e., approximately two-thirds of the gross soil erosion). The SY of the majority (74.2%) of the basin area is low (i.e., ≤5.0 t ha<sup>−1</sup> y<sup>−1</sup>), whereas the soil erosion and transport processes are a major concern in some areas (ca. 6.3 km<sup>2</sup>). The results imply the significance of land use/land cover, rainfall, and topography in controlling the soil erosion risk of the basin. The study demonstrates the advantages of the delineation of the critical erosion areas, based on the hydrologic response units (HRUs) of the basin, to implement appropriate soil erosion management options for conservation of the soil resources.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"8 ","pages":"Article 100072"},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49757135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Assessment of Heavy metal pollution and its health implications in groundwater for drinking purpose around inactive mines, SW region of Cuddapah Basin, South India 印度南部库达帕盆地西南地区闲置矿山周围饮用地下水重金属污染及其健康影响评估
Pub Date : 2023-07-29 DOI: 10.1016/j.totert.2023.100069
Y. Sudharshan Reddy , V. Sunitha

Trace metal leachate comes from inactive mine reservoirs in the study area. The main intention of the study was to analyze the metal content in groundwater samples around inactive mines in the southwest of the Cuddapah Basin and to assess the risk to the local habitants based on calculations using several statistical methods. In this context, the study emphasizes the use of an integrated heavy metal pollution approach, ecological risk assessment incorporating potential health risks, and a multivariate statistical approach at an inactive mining site in the southwestern Cuddapah Basin in southern India. In 2019, we collected 100 samples from bore wells in both pre & post monsoon seasons and ten heavy metals (As, Co, Cd, Fe, Cr, Ni, Mn, Pb, Zn, and Sr) were analyzed using an Agilent 725 ICP-OES instrument. The results showed that As (0–60.5 and 0–56.3 µg/L), Cd (0–28.1 and 0–31.1 µg/L), Ni (0–110.2 and 0–99 µg/L), Pb (0––175.6 and 0–92.3), Sr concentration (0–1150 and 0–1440 μg/L) exceeded permissible values ​​in both seasons. Heavy metal pollution index (HPI), heavy metal evolution index HEI, and pollution degree DOC are used to evaluate the metal pollution of drinking water in this area. As per the HPI values, 17.5 % and 10% of samples show a high pollution index; as per the classes of HEI, 95 % and 100% of samples show a low pollution class during both seasons. According to DOC, all groundwater samples fall in the low contamination zone. As per ecological risk classification, 90% of the samples cause extreme to high ecological risk in both seasons in the study area. From the perspective of health risk assessment, both adults and children have non-carcinogenic effects in the study area; and adequate remedial procedures or treatments are required to avoid metal pollution of groundwater.m.

微量金属渗滤液来源于研究区内不活跃的矿山储层。该研究的主要目的是分析Cuddapah盆地西南部非活动矿山周围地下水样本中的金属含量,并根据几种统计方法的计算结果评估对当地居民的风险。在这种情况下,该研究强调在印度南部库达帕盆地西南部的一个非活动矿场使用综合重金属污染方法、包含潜在健康风险的生态风险评估和多元统计方法。2019年,我们在pre&;使用安捷伦725 ICP-OES仪器对季风后季节和10种重金属(As、Co、Cd、Fe、Cr、Ni、Mn、Pb、Zn和Sr)进行了分析。结果表明,As(0–60.5和0–56.3µg/L)、Cd(0–28.1和0–31.1µg/L),Ni(0–110.2和0–99µg/L)和Pb(0–175.6和0–92.3),Sr浓度(0–1150和0–1440μg/L)超过允许值​​在两个季节。采用重金属污染指数(HPI)、重金属演化指数HEI和污染程度DOC对该地区饮用水的金属污染进行评价。根据HPI值,17.5%和10%的样品显示出高污染指数;根据HEI的等级,95%和100%的样品在两个季节都显示出低污染等级。根据DOC的说法,所有地下水样本都属于低污染区。根据生态风险分类,90%的样本在研究区域的两个季节都会造成极端到高的生态风险。从健康风险评估的角度来看,成人和儿童在研究区域都具有非致癌作用;并且需要足够的补救程序或处理以避免地下水的金属污染。
{"title":"Assessment of Heavy metal pollution and its health implications in groundwater for drinking purpose around inactive mines, SW region of Cuddapah Basin, South India","authors":"Y. Sudharshan Reddy ,&nbsp;V. Sunitha","doi":"10.1016/j.totert.2023.100069","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100069","url":null,"abstract":"<div><p>Trace metal leachate comes from inactive mine reservoirs in the study area. The main intention of the study was to analyze the metal content in groundwater samples around inactive mines in the southwest of the Cuddapah Basin and to assess the risk to the local habitants based on calculations using several statistical methods. In this context, the study emphasizes the use of an integrated heavy metal pollution approach, ecological risk assessment incorporating potential health risks, and a multivariate statistical approach at an inactive mining site in the southwestern Cuddapah Basin in southern India. In 2019, we collected 100 samples from bore wells in both pre &amp; post monsoon seasons and ten heavy metals (As, Co, Cd, Fe, Cr, Ni, Mn, Pb, Zn, and Sr) were analyzed using an Agilent 725 ICP-OES instrument. The results showed that As (0–60.5 and 0–56.3 µg/L), Cd (0–28.1 and 0–31.1 µg/L), Ni (0–110.2 and 0–99 µg/L), Pb (0––175.6 and 0–92.3), Sr concentration (0–1150 and 0–1440 μg/L) exceeded permissible values ​​in both seasons. Heavy metal pollution index (HPI), heavy metal evolution index HEI, and pollution degree DOC are used to evaluate the metal pollution of drinking water in this area. As per the HPI values, 17.5 % and 10% of samples show a high pollution index; as per the classes of HEI, 95 % and 100% of samples show a low pollution class during both seasons. According to DOC, all groundwater samples fall in the low contamination zone. As per ecological risk classification, 90% of the samples cause extreme to high ecological risk in both seasons in the study area. From the perspective of health risk assessment, both adults and children have non-carcinogenic effects in the study area; and adequate remedial procedures or treatments are required to avoid metal pollution of groundwater.m.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"8 ","pages":"Article 100069"},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49730475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Spatio-temporal variations of metals in groundwater from an iron mining impacted area: Assessing sources and human health risk 铁矿开采影响地区地下水中金属的时空变化:评估来源和人类健康风险
Pub Date : 2023-07-29 DOI: 10.1016/j.totert.2023.100070
Soma Giri , Ashwani Kumar Tiwari , Mukesh Kumar Mahato , Abhay Kumar Singh

The concentrations of metals were determined seasonally to assess the probable risk on the local population due to ingestion of metals via the groundwater in an iron mining impacted area of Jharkhand in India. The results depicted significant spatial and temporal variation in the concentration of metals with the highest values during the pre-monsoon season and in the locations with active mining activities. Fe and Mn exceeded the drinking water quality standards in about 75% of the samples considering all the seasons. The metals in the groundwater can be ascribed to both geogenic sources and anthropogenic causes as depicted from principal component analysis, which resulted in extraction of four factors explaining 68.1% of data variance. The hazard quotients (HQ) of the metals calculated for the human health risk assessment according to USEPA methodology suggested that the metals individually did not pose risk to the local population since the HQ of the metals did not exceed unity. However, collective risk of the metals as calculated by the Hazard Index (HI) suggested that the groundwater of most of the locations is unfit for drinking predominantly in the pre monsoon season. The child residents were more susceptible to non-carcinogenic risk than the adult population. The HI for the child population was estimated to be greater than one (1.16) in the pre monsoon season suggesting health risk for vulnerable child populace. The risk was higher in the pre monsoon season as compared to other seasons.

金属浓度是季节性确定的,以评估印度贾坎德邦受铁矿开采影响地区通过地下水摄入金属对当地人口可能造成的风险。结果显示,在季风前季节和采矿活动活跃的地区,金属浓度在空间和时间上存在显著变化,最高值。考虑到所有季节,约75%的样本中Fe和Mn超过了饮用水质量标准。如主成分分析所示,地下水中的金属既可归因于地质来源,也可归因于人为原因,这导致提取了四个因素,解释了68.1%的数据差异。根据美国环保局的方法,为人类健康风险评估计算的金属的危险系数(HQ)表明,由于金属的HQ没有超过1,因此这些金属单独不会对当地人口构成风险。然而,根据危害指数(HI)计算的金属集体风险表明,大多数地区的地下水主要在季风前季节不适合饮用。儿童居民比成年人更容易受到非致癌风险的影响。据估计,雨季前儿童人口的HI大于1(1.16),这表明弱势儿童人口的健康风险。与其他季节相比,季风前季节的风险更高。
{"title":"Spatio-temporal variations of metals in groundwater from an iron mining impacted area: Assessing sources and human health risk","authors":"Soma Giri ,&nbsp;Ashwani Kumar Tiwari ,&nbsp;Mukesh Kumar Mahato ,&nbsp;Abhay Kumar Singh","doi":"10.1016/j.totert.2023.100070","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100070","url":null,"abstract":"<div><p>The concentrations of metals were determined seasonally to assess the probable risk on the local population due to ingestion of metals via the groundwater in an iron mining impacted area of Jharkhand in India. The results depicted significant spatial and temporal variation in the concentration of metals with the highest values during the pre-monsoon season and in the locations with active mining activities. Fe and Mn exceeded the drinking water quality standards in about 75% of the samples considering all the seasons. The metals in the groundwater can be ascribed to both geogenic sources and anthropogenic causes as depicted from principal component analysis, which resulted in extraction of four factors explaining 68.1% of data variance. The hazard quotients (HQ) of the metals calculated for the human health risk assessment according to USEPA methodology suggested that the metals individually did not pose risk to the local population since the HQ of the metals did not exceed unity. However, collective risk of the metals as calculated by the Hazard Index (HI) suggested that the groundwater of most of the locations is unfit for drinking predominantly in the pre monsoon season. The child residents were more susceptible to non-carcinogenic risk than the adult population. The HI for the child population was estimated to be greater than one (1.16) in the pre monsoon season suggesting health risk for vulnerable child populace. The risk was higher in the pre monsoon season as compared to other seasons.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"8 ","pages":"Article 100070"},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49757415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Perspectives on systematic generation of antibiotic resistance with special emphasis on modern antibiotics 对抗生素耐药性系统生成的展望,特别强调现代抗生素
Pub Date : 2023-07-14 DOI: 10.1016/j.totert.2023.100068
Kavya I.K , Nikita Kochhar , Anshika Ghosh , Shrashti Shrivastava , Varunendra Singh Rawat , Soma Mondal Ghorai , Kushneet Kaur Sodhi , Anina James , Mohit Kumar

Antibiotics, considered a “wonder medication” in the 20th century, have been exploited to such an extent that bacteria have developed resistance against them giving rise to highly resistant superbugs, which are able to survive in the presence of a medicine that is designed to stop them from growing. The study elucidates the classification and molecular mechanisms of resistance methods employed by bacteria against different generations of antibiotics with their classes, examples and resistant strains. The overuse, misuse and underuse of several antibiotic classes and their improper disposal/treatment in various industries including agriculture, poultry, veterinary and healthcare has led to the generation of antibiotic resistance in many species of bacteria at a higher rate. The causes, drivers, consequences, and the environmental impact of antibiotic resistance also addressed. This is a major global concern amid the recent rise in infectious diseases caused by antibiotic resistant bacterial pathogens and calls for effective, newer generations of antibiotics and better alternative treatments. If we fail to combat antibiotic resistance, death tolls due to small infections would rise which would further lead to social, economic, environmental and medical losses. This paper highlights the pressing need to curb the overuse of antibiotics to prevent antibiotic resistance, advocates for regulation, awareness programs about AMR and the barriers to further discovery.

抗生素在20世纪被认为是一种“神奇药物”,但它的开发程度已经达到了细菌对抗生素产生耐药性的程度,从而产生了高耐药性的超级细菌,这些细菌能够在旨在阻止它们生长的药物存在下生存。本研究阐明了细菌对不同世代抗生素的耐药性方法的分类和分子机制,以及它们的类别、实例和耐药性菌株。在包括农业、家禽、兽医和医疗保健在内的各个行业中,几种抗生素的过度使用、误用和使用不足,以及它们的不当处置/治疗,导致许多细菌以更高的速度产生抗生素耐药性。抗生素耐药性的原因、驱动因素、后果和环境影响也得到了解决。这是全球关注的一个主要问题,因为最近由抗生素耐药性细菌病原体引起的传染病有所增加,需要有效的新一代抗生素和更好的替代治疗方法。如果我们不能对抗抗生素耐药性,小规模感染导致的死亡人数将上升,这将进一步导致社会、经济、环境和医疗损失。这篇论文强调了遏制抗生素过度使用以防止抗生素耐药性的迫切需要、监管的倡导者、对AMR的认识计划以及进一步发现的障碍。
{"title":"Perspectives on systematic generation of antibiotic resistance with special emphasis on modern antibiotics","authors":"Kavya I.K ,&nbsp;Nikita Kochhar ,&nbsp;Anshika Ghosh ,&nbsp;Shrashti Shrivastava ,&nbsp;Varunendra Singh Rawat ,&nbsp;Soma Mondal Ghorai ,&nbsp;Kushneet Kaur Sodhi ,&nbsp;Anina James ,&nbsp;Mohit Kumar","doi":"10.1016/j.totert.2023.100068","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100068","url":null,"abstract":"<div><p>Antibiotics, considered a “wonder medication” in the 20th century, have been exploited to such an extent that bacteria have developed resistance against them giving rise to highly resistant superbugs, which are able to survive in the presence of a medicine that is designed to stop them from growing. The study elucidates the classification and molecular mechanisms of resistance methods employed by bacteria against different generations of antibiotics with their classes, examples and resistant strains. The overuse, misuse and underuse of several antibiotic classes and their improper disposal/treatment in various industries including agriculture, poultry, veterinary and healthcare has led to the generation of antibiotic resistance in many species of bacteria at a higher rate. The causes, drivers, consequences, and the environmental impact of antibiotic resistance also addressed. This is a major global concern amid the recent rise in infectious diseases caused by antibiotic resistant bacterial pathogens and calls for effective, newer generations of antibiotics and better alternative treatments. If we fail to combat antibiotic resistance, death tolls due to small infections would rise which would further lead to social, economic, environmental and medical losses. This paper highlights the pressing need to curb the overuse of antibiotics to prevent antibiotic resistance, advocates for regulation, awareness programs about AMR and the barriers to further discovery.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"8 ","pages":"Article 100068"},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49730309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Use of treated sewage or wastewater as an irrigation water for agricultural purposes- Environmental, health, and economic impacts 使用处理过的污水或废水作为农业灌溉用水-环境、健康和经济影响
Pub Date : 2023-06-01 DOI: 10.1016/j.totert.2023.100051
Soma Mishra , Rakesh Kumar , Manish Kumar

Reusing sewage water for crop irrigation becomes necessary due to freshwater scarcity, and groundwater depletion. Although the suitability of using treated sewage for crop irrigation remains a topic of argument among government authorities and policymakers. In light of this, this review article summarizes the present global situation and techniques for recycling and application of treated sewage water in agriculture. This paper highlights the environmental, health, and economic impacts of using wastewater as crop irrigation water, along with the advantages and disadvantages are carefully considered. The main objective of this study is to present a global scenario of treated wastewater being used to irrigate agricultural crops, along with the hazards associated with soil fertility, crop production, public health, and economic factors. This study compiled information on the best sewage treatment for agricultural irrigation disinfection practices. Eventually, database of laws and rules governing the use of sewage or wastewater in agricultural irrigation techniques for many countries throughout the world was created. As an environmental impact, effects on water bodies, quality of agricultural soil, microbial communities of soil, and growth of plants have been summarized. For the impact on public health, significant emphasis was given to the exposure to pathogens and heavy metals for both farmers and consumers. The potential risks of utilizing sewage water for human consumption have also been summarised. Focusing on summarizing economic impact involves financial investment and benefits to sewage treatment plants and farmers.

由于淡水短缺和地下水枯竭,有必要将污水重新用于作物灌溉。尽管使用处理过的污水进行作物灌溉的适宜性仍然是政府当局和政策制定者争论的话题。鉴于此,本文综述了处理后的污水在农业中的回收和应用的全球现状和技术。本文强调了将废水用作作物灌溉水对环境、健康和经济的影响,并仔细考虑了其优缺点。本研究的主要目的是介绍一种用于灌溉农作物的经处理废水的全球情景,以及与土壤肥力、作物生产、公共卫生和经济因素相关的危害。这项研究汇编了有关农业灌溉消毒实践中最佳污水处理的信息。最终,建立了世界上许多国家在农业灌溉技术中使用污水或废水的法律和规则数据库。作为一种环境影响,综述了对水体、农业土壤质量、土壤微生物群落和植物生长的影响。关于对公众健康的影响,特别强调了农民和消费者接触病原体和重金属的情况。还总结了将污水用于人类消费的潜在风险。重点总结经济影响涉及到对污水处理厂和农民的财政投资和效益。
{"title":"Use of treated sewage or wastewater as an irrigation water for agricultural purposes- Environmental, health, and economic impacts","authors":"Soma Mishra ,&nbsp;Rakesh Kumar ,&nbsp;Manish Kumar","doi":"10.1016/j.totert.2023.100051","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100051","url":null,"abstract":"<div><p>Reusing sewage water for crop irrigation becomes necessary due to freshwater scarcity, and groundwater depletion. Although the suitability of using treated sewage for crop irrigation remains a topic of argument among government authorities and policymakers. In light of this, this review article summarizes the present global situation and techniques for recycling and application of treated sewage water in agriculture. This paper highlights the environmental, health, and economic impacts of using wastewater as crop irrigation water, along with the advantages and disadvantages are carefully considered. The main objective of this study is to present a global scenario of treated wastewater being used to irrigate agricultural crops, along with the hazards associated with soil fertility, crop production, public health, and economic factors. This study compiled information on the best sewage treatment for agricultural irrigation disinfection practices. Eventually, database of laws and rules governing the use of sewage or wastewater in agricultural irrigation techniques for many countries throughout the world was created. As an environmental impact, effects on water bodies, quality of agricultural soil, microbial communities of soil, and growth of plants have been summarized. For the impact on public health, significant emphasis was given to the exposure to pathogens and heavy metals for both farmers and consumers. The potential risks of utilizing sewage water for human consumption have also been summarised. Focusing on summarizing economic impact involves financial investment and benefits to sewage treatment plants and farmers.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"6 ","pages":"Article 100051"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49707519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Perspectives of heavy metal pollution indices for soil, sediment, and water pollution evaluation: An insight 重金属污染指标在土壤、沉积物和水污染评价中的应用前景
Pub Date : 2023-06-01 DOI: 10.1016/j.totert.2023.100039
Bhairo Prasad Ahirvar , Pallavi Das , Vaibhav Srivastava , Manish Kumar

The paper reviews the widely used heavy metal pollution indices (HPIs) in water, soil, and sediments and presents their numerical equation and application in various fields. HPIs are an essential and efficient tool to measure metal contamination in the environmental system. The present study includes twenty pollution indices from water, soil, and sediments for their comparative study with their ranges. In addition, the regional distribution of publications has been reviewed, and it observed that 68% were published from Asia, followed by other continents. The merits and demerits of each index have been presented, and a comprehensive method for selecting the appropriate pollution index is evaluated in order to better interpret the pollution level in soils and water systems. Among water quality indices HPI, HEI and HI have been used widely due to precise scale, easy to use, less limitations and provide efficient results. Among single indices, EF, Igeo, and CF are widely used and can be used in a simple manner for soil quality assessment, whereas in combined indices PLI and INemerow are found to be useful as it is easy to apply and have precise scale. In integrated indices, Cd and IAvg are found to be useful as it is easy to apply, and there is no limitation for heavy metals. Selection of appropriate geochemical background (GB) must be established, and the nature of fluctuation in GB may give a false result. The ecological risk index (ERI) is a unique index used to represent the ecological risk of metal pollution in water, soil, and sediment systems. A comprehensive approach and guidelines for using metal pollution indices in soil and sediment considering different sectors may be developed for each nation. The widespread application of HPI makes the study more accessible, and a well-defined result can be presented.

综述了水、土壤和沉积物中广泛使用的重金属污染指数,并介绍了它们的数值方程及其在各个领域的应用。HPI是测量环境系统中金属污染的重要而有效的工具。本研究包括20个来自水、土壤和沉积物的污染指数,用于与它们的范围进行比较研究。此外,还对出版物的区域分布进行了审查,发现68%的出版物来自亚洲,其次是其他大陆。介绍了各指标的优缺点,并对选择适当污染指数的综合方法进行了评估,以更好地解释土壤和水系的污染水平。在水质指标中,HPI、HEI和HI由于尺度精确、易于使用、局限性小、结果有效而得到广泛应用。在单一指数中,EF、Igeo和CF被广泛使用,并且可以以简单的方式用于土壤质量评估,而在组合指数中,PLI和INemerow被发现是有用的,因为它易于应用并且具有精确的尺度。在综合指数中,Cd和IAvg很有用,因为它很容易应用,而且对重金属没有限制。必须确定适当的地球化学背景(GB)的选择,GB波动的性质可能会给出错误的结果。生态风险指数(ERI)是一个独特的指数,用于表示水、土壤和沉积物系统中金属污染的生态风险。考虑到不同部门,可以为每个国家制定一种使用土壤和沉积物中金属污染指数的综合方法和指南。HPI的广泛应用使该研究更容易获得,并且可以给出明确的结果。
{"title":"Perspectives of heavy metal pollution indices for soil, sediment, and water pollution evaluation: An insight","authors":"Bhairo Prasad Ahirvar ,&nbsp;Pallavi Das ,&nbsp;Vaibhav Srivastava ,&nbsp;Manish Kumar","doi":"10.1016/j.totert.2023.100039","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100039","url":null,"abstract":"<div><p>The paper reviews the widely used heavy metal pollution indices (HPIs) in water, soil, and sediments and presents their numerical equation and application in various fields. HPIs are an essential and efficient tool to measure metal contamination in the environmental system. The present study includes twenty pollution indices from water, soil, and sediments for their comparative study with their ranges. In addition, the regional distribution of publications has been reviewed, and it observed that 68% were published from Asia, followed by other continents. The merits and demerits of each index have been presented, and a comprehensive method for selecting the appropriate pollution index is evaluated in order to better interpret the pollution level in soils and water systems. Among water quality indices HPI, HEI and HI have been used widely due to precise scale, easy to use, less limitations and provide efficient results. Among single indices, EF, I<sub>geo</sub>, and CF are widely used and can be used in a simple manner for soil quality assessment, whereas in combined indices PLI and I<sub>Nemerow</sub> are found to be useful as it is easy to apply and have precise scale. In integrated indices, C<sub>d</sub> and I<sub>Avg</sub> are found to be useful as it is easy to apply, and there is no limitation for heavy metals. Selection of appropriate geochemical background (GB) must be established, and the nature of fluctuation in GB may give a false result. The ecological risk index (ERI) is a unique index used to represent the ecological risk of metal pollution in water, soil, and sediment systems. A comprehensive approach and guidelines for using metal pollution indices in soil and sediment considering different sectors may be developed for each nation. The widespread application of HPI makes the study more accessible, and a well-defined result can be presented.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"6 ","pages":"Article 100039"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49757117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Potential role of Pseudomonas fluorescens c50 and Sphingobium yanoikuyae HAU in enhancing bioremediation of persistent herbicide atrazine and its toxic metabolites from contaminated soil 荧光假单胞菌c50和刺槐藻HAU在加强污染土壤中持久性除草剂阿特拉津及其毒性代谢物的生物修复中的潜在作用
Pub Date : 2023-06-01 DOI: 10.1016/j.totert.2023.100052
Anil Duhan , Priyanka Bhatti , Ajay Pal , Jagdish Parshad , Ravi Kumar Beniwal , Deepika Verma , Dharam Bir Yadav

This research aimed to decipher the potential utility of Pseudomonas fluorescens strain c50 and Sphingobium yanoikuyae strain HAU in biodegradation and the mechanism of biochemical events occurring during the metabolic route of the herbicide atrazine. The bacterial strains c50 and HAU were isolated and identified based on 16S rRNA gene sequence analysis as P. fluorescens (accession no. OM838401) and S. yanoikuyae (accession no. OM830303), respectively. Both strains could survive in a concentration as high as 200 µg/mL of AT. Desethyl atrazine (DEA), desisopropyl atrazine (DIA), and desethylhydroxy atrazine (DEHA) were three critical metabolites produced during catabolism and quantified using GC–MS/MS. In culture medium, both strains effectively removed AT and its metabolites within 30 d with half-lives ranging from 2 to 9.9 d. Strain c50 performed better than HAU in rapid removal of AT and metabolites. In natural soil without microbial treatment, AT, DEA, DIA and DEHA persisted for 120, 90, 60 and 90 d, respectively with half-lives ranging from 12.1 to 26 d. Application of c50 and HAU accelerated degradation in soil. Persistence time of AT, DEA, DIA and DEHA was reduced to 25–30 d with half-lives ranging from 2.8 to 5.1 d. Strain HAU performed marginal better during biodegradation in soil. Performance of both the strains together was found at par. First-order dissipation kinetics was observed during biodegradation. Increase in microbial biomass carbon from 230 to 670 mg/kg soil indicated about initial surge (150 times) in bacterial population in treated soil and rapid biodegradation of contaminants using later as a food source. This study further revealed that identification of almost all major metabolites and intermediate products is possible using GC–MS/MS. However, reliable quantification of only DEA, DIA, and DEHA is possible without derivatization. Both bacteria could provide eco-friendly alternatives for attenuation of these toxicants from the contaminated soils.

本研究旨在揭示荧光假单胞菌菌株c50和黄曲霉菌株HAU在生物降解中的潜在效用,以及除草剂阿特拉津代谢过程中发生的生化事件的机制。分离菌株c50和HAU,并基于16S rRNA基因序列分析分别鉴定为荧光假单胞菌(登录号OM838401)和yanoikuyae链霉菌(登录号OM 830303)。这两种菌株都可以在高达200µg/mL的AT浓度下存活。去乙基阿特拉津(DEA)、去异丙基阿特拉津和去乙基羟基阿特拉津是分解代谢过程中产生的三种关键代谢产物,并使用GC–MS/MS进行定量。在培养基中,两个菌株在30天内有效地去除了AT及其代谢物,半衰期为2~9.9天。菌株c50在快速去除AT及其代谢物方面表现优于HAU。在未经微生物处理的天然土壤中,AT、DEA、DIA和DEHA的半衰期分别为120、90、60和90d,半衰期为12.1至26d。c50和HAU的应用加速了土壤的降解。AT、DEA、DIA和DEHA的持续时间缩短至25-30 d,半衰期在2.8-5.1 d之间。菌株HAU在土壤中的生物降解过程中表现略好。两种菌株的性能基本一致。在生物降解过程中观察到一级耗散动力学。微生物生物量碳从230毫克/公斤土壤增加到670毫克/公斤,这表明处理过的土壤中的细菌数量最初激增(150倍),污染物随后作为食物来源迅速生物降解。这项研究进一步表明,使用GC–MS/MS可以鉴定几乎所有主要代谢产物和中间产物。然而,只有DEA、DIA和DEHA的可靠量化是可能的,而无需衍生化。这两种细菌都可以为减少污染土壤中的这些有毒物质提供环保的替代品。
{"title":"Potential role of Pseudomonas fluorescens c50 and Sphingobium yanoikuyae HAU in enhancing bioremediation of persistent herbicide atrazine and its toxic metabolites from contaminated soil","authors":"Anil Duhan ,&nbsp;Priyanka Bhatti ,&nbsp;Ajay Pal ,&nbsp;Jagdish Parshad ,&nbsp;Ravi Kumar Beniwal ,&nbsp;Deepika Verma ,&nbsp;Dharam Bir Yadav","doi":"10.1016/j.totert.2023.100052","DOIUrl":"https://doi.org/10.1016/j.totert.2023.100052","url":null,"abstract":"<div><p>This research aimed to decipher the potential utility of <em>Pseudomonas fluorescens</em> strain c50 and <em>Sphingobium yanoikuyae</em> strain HAU in biodegradation and the mechanism of biochemical events occurring during the metabolic route of the herbicide atrazine. The bacterial strains c50 and HAU were isolated and identified based on 16S rRNA gene sequence analysis as <em>P. fluorescens</em> (accession no. OM838401) and <em>S. yanoikuyae</em> (accession no. OM830303), respectively. Both strains could survive in a concentration as high as 200 µg/mL of AT. Desethyl atrazine (DEA), desisopropyl atrazine (DIA), and desethylhydroxy atrazine (DEHA) were three critical metabolites produced during catabolism and quantified using GC–MS/MS. In culture medium, both strains effectively removed AT and its metabolites within 30 d with half-lives ranging from 2 to 9.9 d. Strain c50 performed better than HAU in rapid removal of AT and metabolites. In natural soil without microbial treatment, AT, DEA, DIA and DEHA persisted for 120, 90, 60 and 90 d, respectively with half-lives ranging from 12.1 to 26 d. Application of c50 and HAU accelerated degradation in soil. Persistence time of AT, DEA, DIA and DEHA was reduced to 25–30 d with half-lives ranging from 2.8 to 5.1 d. Strain HAU performed marginal better during biodegradation in soil. Performance of both the strains together was found at par. First-order dissipation kinetics was observed during biodegradation. Increase in microbial biomass carbon from 230 to 670 mg/kg soil indicated about initial surge (150 times) in bacterial population in treated soil and rapid biodegradation of contaminants using later as a food source. This study further revealed that identification of almost all major metabolites and intermediate products is possible using GC–MS/MS. However, reliable quantification of only DEA, DIA, and DEHA is possible without derivatization. Both bacteria could provide eco-friendly alternatives for attenuation of these toxicants from the contaminated soils.</p></div>","PeriodicalId":101255,"journal":{"name":"Total Environment Research Themes","volume":"6 ","pages":"Article 100052"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49707683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
期刊
Total Environment Research Themes
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1