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Plant Parasitic Nematodes: A Major Constraint in Fruit Production 植物寄生线虫:制约果实生产的主要因素
Pub Date : 2022-01-17 DOI: 10.5772/intechopen.101696
Nishi Keshari, Gurram Mallikarjun
The plant parasitic nematodes are one of the major limiting factors in fruit trees specially in citrus, banana, papaya, jackfruit, guava etc. The root knot nematodes are the major problem amongst all those nematodes infecting on these trees. Besides, directly causing a huge losses, they are also inviting the secondary plant pathogens, like fungi, bacteria, viruses etc. amongst which, the wilt fungus, Fusarium species increase the severity of the diseases. This complex disease is becoming much severe in banana and guava recent years. In citrus also, the citrus nematodes, Tylenchulus semipenetrans, is causing havoc by slow decline disease and it is becoming a major problem in horticultural nurseries because these nurseries are a hot spot of citrus nematodes. So, unknowingly these nematodes get spread to different places. The management of these nematodes by simple, cheap and eco friendly methods, is very important as it will decrease the monetary pressure on cultivators as well as it helps in improving environmental pollution.
植物寄生线虫是制约果树生长的主要因素之一,尤其是柑橘、香蕉、木瓜、菠萝蜜、番石榴等。根结线虫是所有感染这些树木的线虫中的主要问题。除直接造成巨大损失外,还引入了真菌、细菌、病毒等次生植物病原体,其中枯萎菌、镰刀菌等增加了病害的严重程度。近年来,这种复杂的疾病在香蕉和番石榴中变得越来越严重。柑橘线虫(Tylenchulus semipenetrans)是柑橘线虫的一种慢衰病,在柑橘苗圃中造成了严重的危害,因为苗圃是柑橘线虫的热点。因此,这些线虫不知不觉地传播到不同的地方。通过简单、廉价和环保的方法来管理这些线虫是非常重要的,因为它将减少种植者的经济压力,并有助于改善环境污染。
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引用次数: 3
Root-Knot Nematodes a Major Peril to Protected Cultivation System in India: Current Status and its Management 根结线虫是印度保护地栽培系统的主要危害:现状及其管理
Pub Date : 2021-10-31 DOI: 10.5772/intechopen.100541
Jaydeep A. Patil, S. Yadav
Growing of vegetable crops under protected conditions are relatively, an innovative technology and most popular among farmers throughout the country. In last few decades protected cultivation has shown potential enhancement in horticultural production. The southern root-knot nematode, Meloidogyne incognita, is an emerging nematode under protected conditions. This nematode can cause chlorosis, stunting and reduce yields associated with the induction of many root galls on host plants. Root-knot nematode severely affect the plant root system by inducing specialized feeding cells i.e., giant cells in the vascular tissues. Recently, this nematode has been considered as a worldwide menace for combat root-knot nematodes, integrated nematode management strategies such as soil solarization, biological control, organic amendment, crop rotation, field sanitation, and fumigants have been developed and successfully used in the past. Here, in this book chapter discussed on biology and life cycle, control measures and proposed future strategies to improve Megalaima incognita management under protected conditions.
在保护条件下种植蔬菜作物是一项相对创新的技术,在全国范围内最受农民欢迎。在过去的几十年里,保护栽培在园艺生产方面显示出潜在的提高潜力。南方根结线虫(Meloidogyne incognita)是一种在保护条件下出现的新型线虫。这种线虫可以引起黄化,发育迟缓和减少产量与诱导许多根瘿在寄主植物上。根结线虫通过诱导维管组织中的特化摄食细胞,即巨细胞,严重影响植物根系。近年来,这种线虫被认为是世界范围内的一种威胁,对线虫的综合管理策略,如土壤日晒、生物防治、有机改良剂、作物轮作、田间卫生和熏蒸,已经开发并成功应用。在这里,本章讨论了在保护条件下,提高隐翅巨蜥管理的生物学和生命周期,控制措施和提出了未来的策略。
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引用次数: 0
Molecular Characterization and Pathogenicity of Trichoderma Isolates to Meloidogyne javanica 木霉分离株的分子特征及致病性
Pub Date : 2021-10-09 DOI: 10.5772/intechopen.99035
Ricardo R. Balardin, C. Bellé, Daiane Dalla Nora, Rodrigo F. Ramos, José Carlos V. Rodrigues, Zaida I. Antoniolli
Nematodes are considered a serious problem for agriculture. Nematodes of the Meloidogyne genus can attack a wide range of plants, needing different management methods to decrease its population. Fungi from the Trichoderma genus has been related to have potential as biological control agents. However, before an organism is used as biological control agent, first it is necessary to prospect, characterize and test its potential as biocontrol agent, so the objective of this work was to characterize and test fungi isolates of the Trichoderma genus to control M. javanica. We obtained forty isolate to carry out this experiment. We extracted the DNA of each isolate to discover which species we were testing, by doing a PCR and sequencing. We tested in vitro their parasitism effect using ELISA plate. Also, we extracted their filtrate to see if their metabolites have potential to reduce nematode population by showing a high mortality or inhibiting hatching. The results confirmed the high potential of the fungi of Trichoderma genus as a biological agent to control Meloidogyne javanica.
线虫被认为是农业的一个严重问题。丝状线虫属的线虫可以攻击广泛的植物,需要不同的管理方法来减少其数量。木霉属真菌被认为具有作为生物防治剂的潜力。然而,在将一种生物用作生物防治剂之前,首先需要对其作为生物防治剂的潜力进行研究、表征和测试,因此本研究的目的是对木霉属真菌分离株进行表征和测试,以防治爪哇木霉。我们获得了40个分离物来进行这个实验。我们提取每个分离物的DNA,通过PCR和测序来发现我们要测试的物种。采用酶联免疫吸附法(ELISA)检测其体外寄生效果。此外,我们提取了它们的滤液,看看它们的代谢物是否有潜力通过显示高死亡率或抑制孵化来减少线虫种群。结果表明,木霉属真菌具有很强的生物防治潜力。
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引用次数: 2
Entomopathogenic Nematodes: Their Characterization, Bio-Control Properties and New Perspectives 昆虫病原线虫:它们的特征、生物防治特性和新观点
Pub Date : 2021-09-07 DOI: 10.5772/intechopen.99319
H. Sharma, A. Rana, Aashaq H. Bhat, Ashok K. Chaubey
The insect parasitoid nematodes are a means boon to agronomy and serve as important bio-pesticides for controlling crop damaging insect pests. These nematodes inhabit moist soils and have been to exist in all the continents excluding Polar Regions. These nematodes have 3rd larval stage infective which is the only free living stage existing outside the host. These infective stages are mutually associated with bacteria which reside in their alimentary canal and duo are responsible for mortality of the insect host. These nematodes are currently given great attention by scientific community because of their insect killing properties and can be used to replace hazardous pesticides. These nematodes include various species belonging to genus Heterorhabditis and Steinernema, and members of insectivorous group of genus Oscheius. Before their use as bio-control agents, these nematodes need to be properly identified. Currently, these nematodes are characterized by using morphological and morphometrical parameters and advanced molecular tools including cross hybridization and scanning electron microscope studies. Their associated bacterial partners are studied through advanced molecular and biochemical techniques. The properly characterized nematodes having more entomopathogenic properties can be easily mass produced through in vitro and in vivo methods. They can be formulated in various carrier materials and supplied to farmers for effective control of damaging insect pests. Several countries have formulated various useful products of entomopathogenic nematodes which are available in markets for use by the farmer community and some have given very effective results. India is still at the early stage in the use of these nematodes for bio-control of insects in agronomy. More research in this field needs to be carried, especially in India to produce effective indigenous nematode products which may prove a boon for agriculture.
拟寄生线虫是农艺学的一种重要手段,是防治作物有害害虫的重要生物农药。这些线虫栖息在潮湿的土壤中,在除极地地区以外的所有大陆都存在。这些线虫有第三幼虫期感染,这是唯一存在于宿主外的自由生活期。这些感染阶段与寄生在其消化道中的细菌相互关联,并对昆虫宿主的死亡负责。这些线虫因其杀虫特性和可替代有害农药而受到科学界的高度重视。这些线虫包括属于Heterorhabditis和Steinernema属的各种物种,以及Oscheius属食虫组的成员。在将这些线虫用作生物防治剂之前,需要对它们进行适当的鉴定。目前,这些线虫是利用形态学和形态计量学参数和先进的分子工具,包括交叉杂交和扫描电镜研究。通过先进的分子和生化技术研究它们的相关细菌伙伴。通过体外和体内的方法,可以很容易地批量生产具有更多昆虫病原性的线虫。它们可以配制成各种载体材料,供应给农民,以有效控制有害害虫。一些国家制定了各种有用的昆虫病原线虫产品,可在市场上获得,供农民社区使用,有些国家取得了非常有效的结果。在利用这些线虫进行农学昆虫生物防治方面,印度仍处于早期阶段。需要在这个领域进行更多的研究,特别是在印度,以生产有效的本土线虫产品,这可能证明是农业的福音。
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引用次数: 1
Entomopathogenic Nematodes: Biological Model of Studies with Anthelmintics 昆虫病原线虫:用驱虫学研究的生物学模型
Pub Date : 2021-09-05 DOI: 10.5772/intechopen.99663
O. Barrón-Bravo, Ismael Montiel-Maya, A. Cruz-Avalos, F. Avila-Ramos, Jaime Molina Ochoa, C. Ángel-Sahagún
Anthelmintics used in animals to combat parasitic infections are mainly excreted in manure and cause negative effects on the environment and decomposers. Nematodes are associated with the rhizosphere; some are gastrointestinal parasites of animals, and others regulate insects and other arthropods (entomopathogenic nematodes) and are considered beneficial. The habitat and the similarities that exist among them give the opportunity to use nematodes as a biological model. The availability of target organisms is not always feasible; therefore, experimental studies with models similar to those of the target organisms are a possibility. In veterinary clinics, the study of drug susceptibility is a fundamental tool to monitor the development of resistance. To conserve the biodiversity of the environment, it is necessary to make adequate use of anthelmintics, avoid resistance to these pesticides and prevent the used products from damaging populations of beneficial organisms.
用于动物对抗寄生虫感染的驱虫药主要随粪便排出,对环境和分解者造成负面影响。线虫与根际有关;一些是动物的胃肠道寄生虫,另一些调节昆虫和其他节肢动物(昆虫病原线虫),被认为是有益的。栖息地和它们之间存在的相似性为使用线虫作为生物模型提供了机会。目标生物的可用性并不总是可行的;因此,使用与目标生物相似的模型进行实验研究是可能的。在兽医诊所,药物敏感性研究是监测耐药性发展的基本工具。为了保护环境的生物多样性,必须充分使用驱虫剂,避免对这些杀虫剂产生抗药性,并防止使用的产品损害有益生物种群。
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引用次数: 1
Nematodes as Biological Indicators of Soil Quality in the Agroecosystems 线虫作为农业生态系统土壤质量的生物学指标
Pub Date : 2021-09-04 DOI: 10.5772/intechopen.99745
T. Khanum, N. Mehmood, N. Khatoon
Soil nematodes have advantages as bio-indicators, because they have beneficial role in the food web. Nematodes associated with bacteria are probably the most studied biological indicators of soil fertility. Saprophytic nematodes act as bio-indicators of soil health because they have different beneficial ways to increase in soil functions such as in management of ecosystem; enhancement of nitrogen in soil by ingestion of nitrogen and secrete extra nitrogen as NH4, that is easily absorbable; putrefaction and by dispersion of bacteria and fungi to recently available organic residues. Therefore, nematode are beneficial in increasing soil health or plant growth by providing the nutrient through associated bacteria. So it can be evaluated that the nematodes use as biological indicators of soil fertility because of remarkable diversity and nematode contribution in many functions of the soil fertility.
土壤线虫在食物网中发挥着有益的作用,作为生物指示物具有一定的优势。与细菌相关的线虫可能是研究最多的土壤肥力的生物指标。腐生线虫是土壤健康的生物指标,因为它们有不同的有益途径来增加土壤的功能,如生态系统管理;通过吸收氮提高土壤中的氮含量,并以NH4的形式分泌多余的氮,易于吸收;腐烂和细菌和真菌分散到最近可用的有机残留物。因此,线虫通过相关的细菌提供养分,有利于土壤健康或植物生长。线虫具有显著的多样性,在土壤肥力的许多功能中都有贡献,因此可以评价线虫作为土壤肥力的生物学指标。
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引用次数: 2
Effects of Irrigation and Bioproducts of Microbial Origin on Nematode Community and Mycorrhizal Root Colonization in Soybean 灌溉和微生物源生物制品对大豆线虫群落和菌根定植的影响
Pub Date : 2021-08-11 DOI: 10.5772/intechopen.99294
I. Majić, A. Sarajlić, E. Raspudić, M. Josipović, G. Šarić
Soybean (Glycine max L. Merr) is the most important legume and threaten by diverse pests and diseases. Complex interactions among rhizosphere organisms are found in all agro-ecosystems. Results of these interactions can be positive and/or negative in terms of plant production. Soil nematode community consists of different trophic groups of nematodes. Nematodes are the most abundant soil invertebrates. Several nematode species penetrate soybean roots as parasites, and can cause loss in yields. Arbuscular mycorrhiza fungi are obligate plant symbionts that colonize soybean roots naturally. The aim of the study was to evaluate effects of irrigation and amendments of bioproducts containing beneficial soil microorganisms (ABM) on nematode community and mycorrhizal root colonization in soybean. Field experiments were conducted in soybean in 2013 in Osijek, Croatia. The plots were either rain fed or irrigated to 60-100% field water capacity (FWC). We tested soil amendments and soil + foliar amendments of three commercial products containing beneficial organisms. Average number of nematodes per soil sample varied from 186,67 (soil ABM in non-irrigated plots) to 297,57 (soil+foliar ABM in plots with 60-100% FWC), and there were no significant differences between the treatments. Bacterial feeding nematodes were the most abundant, while plant parasitic genus Pratylenchus was the most abundant among other plant parasitic nematodes. There was no clear influence of any of the treatments on soil nematode community. Amendments of the bioproducts increased mycorrhizal root colonization in rain fed plots, while it decreased the mycorrhizal root colonization when soybeans were irrigated. Irrigation increased mycorrhizal root colonization in plots without amendments of the bioproducts, and mycorrhizal colonization differed significantly between the sampling dates. Further research is needed to determine if irrigation alters the potential of mycorrhiza to colonize the roots.
大豆(Glycine max L. Merr)是最重要的豆科植物,受到多种病虫害的威胁。在所有农业生态系统中,根际生物之间都存在复杂的相互作用。就植物生产而言,这些相互作用的结果可能是积极的和/或消极的。土壤线虫群落由不同营养类群的线虫组成。线虫是最丰富的土壤无脊椎动物。几种线虫以寄生虫的形式侵入大豆根系,造成产量损失。丛枝菌根真菌是专性植物共生体,自然定植在大豆根部。摘要本研究旨在探讨灌溉和含有益土壤微生物(ABM)生物制品改良对大豆线虫群落和菌根定植的影响。2013年在克罗地亚奥西耶克进行了大豆田间试验。这些地块采用雨养或灌溉,灌溉水量达到60-100%。我们测试了三种含有有益生物的商业产品的土壤改良剂和土壤+叶面改良剂。每样土壤样品平均线虫数为186,67(未灌溉区土壤ABM) ~ 297,57(灌溉区土壤+叶面ABM),处理间差异不显著。细菌取食线虫数量最多,而植物寄生属(Pratylenchus)在其他植物寄生线虫中数量最多。各处理对土壤线虫群落的影响均不明显。生物制品的添加增加了雨养田中菌根的定植,而减少了大豆灌溉田中菌根的定植。在未添加生物制品的情况下,灌溉水增加了菌根定植,且不同采样日期间菌根定植差异显著。需要进一步的研究来确定灌溉是否会改变菌根在根部定殖的潜力。
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引用次数: 0
Management of Root-Knot Nematode, Meloidogyne Incognita Dreaded Invading in Pointed Gourd (Trichosanthes dioica Roxb.) Crop Prone to Eastern U.P of India 尖葫芦根结线虫入侵的防治作物倾向于印度北方邦东部
Pub Date : 2021-08-03 DOI: 10.5772/intechopen.98923
A. Anwar, Najeeb Mughal, Efath Shahnaz, S. Banday, Taibah Bashir, Qadrul Nisa, G. Jeelani
Pointed gourd belongs to cucurbitaceae family and is extensively cultivated in eastern Uttar Pradesh (10000 Hectares), Bihar (14000 hectares), West Bengal, Assam, Orissa, Madhya Pradesh, Maharashtra and Gujrat. Its plants are perennial in nature and can survive for several years even if left uncared. This crop occupies large area of land in India. The system of cultivation varies from region to region such as trained on pandals or ardours especially during the rainy season in southern and western India. However, it is most susceptible to root-knot nematode, Meloidogyne incognita, the population level or density of root-knot nematodes were found in the range of 15–100 per cent of the root and soil samples. This nematode induces severe damage to pointed gourd on coarse-textured sandy soils, particularly during droughts stress. Crop failure is noticed at earlier stage of vines. In view of fact it is necessary to evolve the integrated strategies for management of root-knot nematode in this viny crop.
尖头葫芦属于葫芦科,广泛种植于北方邦东部(10000公顷)、比哈尔邦(14000公顷)、西孟加拉邦、阿萨姆邦、奥里萨邦、中央邦、马哈拉施特拉邦和古吉拉特。它的植物是多年生的,即使不加照料,也能存活数年。这种作物在印度占据了大片土地。种植系统因地区而异,例如在熊猫或热情训练,特别是在印度南部和西部的雨季。然而,它最容易感染根结线虫,根结线虫的种群水平或密度在根和土壤样品的15 - 100%范围内。这种线虫对粗质沙质土壤上的尖葫芦造成严重损害,特别是在干旱胁迫期间。在葡萄藤的早期阶段就可以注意到作物的歉收。鉴于这一事实,有必要发展根结线虫的综合管理策略。
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引用次数: 1
Biological Control of Root-Knot Nematodes Using Trichoderma Spp. 木霉对根结线虫的生物防治。
Pub Date : 2021-07-28 DOI: 10.5772/intechopen.99218
Linnley Mulusa
Agriculture is an important activity globally since it ensures food security and is a source of income for many families, especially those living in underprivileged countries. The continuous growth in the global population has seen farmers increase the crop production acreage to meet the increasing demand for food and avert food shortage. Despite this, farmers continue to harvest lower yields than anticipated, which threatens global food security. The reduced yields result from outdated and ineffective farming practices as well as pests and diseases. Diseases are a significant cause of reduced crop yields globally. Biotic and abiotic factors cause diseases. Of the recognized biotic causes of disease, root-knot nematodes, also known as Meloidogyne spp. are plant-parasitic nematodes that cause significant losses to farmers in terms of reduced plant yields. Over the years, researchers have conducted several studies on the effective use of Trichoderma spp. fungi as a biocontrol agent for these pathogens. This paper analyzes the advancements made towards the effective and efficient biocontrol of Meloidogyne spp. using Trichoderma spp. and the implications of these advancements for agriculture and food security.
农业是全球的一项重要活动,因为它确保粮食安全,是许多家庭的收入来源,特别是生活在贫困国家的家庭。随着全球人口的持续增长,农民增加了农作物种植面积,以满足日益增长的粮食需求,避免粮食短缺。尽管如此,农民的收成仍然低于预期,这威胁到全球粮食安全。产量下降是由于过时和无效的耕作方法以及病虫害造成的。疾病是全球农作物减产的一个重要原因。生物和非生物因素引起疾病。在公认的生物致病原因中,根结线虫,也称为根结线虫属,是植物寄生线虫,对农民造成重大损失,使植物产量下降。多年来,研究人员对木霉真菌作为这些病原体的生物防治剂的有效利用进行了多项研究。本文分析了利用木霉有效防治甜霉病的研究进展及其对农业和粮食安全的意义。
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引用次数: 1
The Emerging Nematode Problems in Horticultural Crops and their Management 园艺作物中新出现的线虫问题及其管理
Pub Date : 2021-07-28 DOI: 10.5772/intechopen.99292
S. Yadav, J. Patil
Plant-parasitic nematodes (PPNs) are responsible for significant monetary losses to horticultural crops. They are unseen foes of crops and devitalize plants by causing injury to plant roots or aboveground parts. From last few decades, increased attention has been paid to nematode problems in horticultural crops in open as well as under protected cultivation. PPNs are obligate parasites, mostly have wide host range and are widespread pathogens of horticultural crops. The dimension of damage is density dependent and their management options vary with type of crop, nematode species and other factors. Recent approaches to combat losses caused by nematodes are the use of nematicides, cultural practices and resistant cultivars that may be used singly or in an integrated manner. This book chapter gives an overview of the emerging nematode problems in horticultural crops and their management strategies.
植物寄生线虫(ppn)对园艺作物造成重大经济损失。它们是农作物看不见的敌人,通过对植物根部或地上部分造成伤害而使植物失去生命。近几十年来,园艺类作物的线虫问题越来越受到人们的重视。ppn是专性寄生虫,寄主范围广,是园艺作物的广泛病原菌。损害程度与密度有关,其管理方案因作物类型、线虫种类和其他因素而异。防治线虫造成的损失的最新方法是使用杀线虫剂、栽培方法和抗性品种,这些方法可以单独使用,也可以综合使用。本章概述了园艺作物中出现的线虫问题及其管理策略。
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引用次数: 0
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