Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles最新文献

Lipidoperoxidation in the sebum from 10 subjects suffering from inflammatory acne has been shown by use of mass spectroscopy, in contrast with 10 normal subjects where this was not observed. Peroxidation of sebum lipids is probably linked with the inflammation of the comedons.

The diameter of inhibition zones produced by discs of aminoglycosides in cultures of Escherichia coli varied on different Mueller Hinton media (Pasteur, Merck). The minimum inhibitory concentration (M.I.C.) estimated was 20 times greater with Pasteur medium compared to Merck medium. This increase of the M.I.C. was related to the presence of glucose in the medium.

A chemiluminescent procedure to measure acetylcholine was developed and used to detect continuously the release of transmitter from stimulated electric organ slices and synaptosomes. Acetylcholine is hydrolysed by acetylcholinesterase; the choline liberated is oxidized by choline oxidase to betaïne; the H2O2 generated triggers the luminescence of luminol in presence of peroxidase. The extension to other transmitters is possible; several developments are envisaged.

A physiological analysis of an E. coli K 12 periplasmic-leaky mutant shows that specific excretion of periplasmic proteins increases with time and temperature of incubation when cells are grown in rich L medium. The associated lky mutation was mapped by use of conventional genetic techniques near the gal locus.

In primary cultures the free nucleotide (FN) distribution pattern showed a slight increase of adenylic nucleotides in neuroblasts as compared to glioblasts. Significant differences in the FN distribution were found between primary cultures and clonal nerve cell lines.

Two strains of parasites isolated from cases of oriental sore in the Pyrénées-Orientales were identified by electrophoretic analysis of eight isoenzymes (PGM, PGI, G-6-PDH, 6-PGDH, IDH, MDH, ME and GOT). Both strains were identical with Leishmania infantum Nicolle, 1908, which normally causes visceral leishmaniasis. It is probable that, in the Mediterranean region, some cutaneous lesions observed within the area of visceral leishmaniasis are caused by this species rather than by Leishmania tropica (Wright, 1903) or Leishmania major (Yakimoff and Schokor, 1914) traditionally believed to be agents of cutaneous leishmaniasis in the Old World.

A rise in erythrocyte Na+ content secondary to a sodium rich diet has been observed in genetically hypertensive Rats and in Rats with a predisposition to develop hypertension under the influence of Na+ loading. In contrast, such a rise is absent in Rats with a genetic resistance to the development of hypertension. Hence, the determination of erythrocyte Na+ after exposure to a sodium load may permit the characterization of primary hypertension.

The binding capacity of the plasma testosterone binding globulin (TeBG) as determined by the equilibrium dialysis method, in fox and badger, was shown to wary in relation to the seasonal endocrine activity of the testis. During the winter breeding period, the TeBG binding capacity was low in the fox and high in the badger, whereas an opposite situation prevailed during the sexual quiescence phase. Independent of those seasonal variations, the binding capacity of TeBG was always lower in fox than in badger.

Structural modifications of enkephalins obtained by complexation of Zn++ are brought out by high resolution 1 H N M R spectroscopy. For one of the two enkephalins studied, the conformational equilibrium is greatly shifted by the introduction of the metal ion.

A single dose (120 mg/kg IM) of testosterone administered 1 month before subtotal hepatectomy (90%) in Rats, reduced liver steatosis and allowed complete liver regeneration.