Pub Date : 2023-09-22DOI: 10.1007/s12686-023-01321-7
Xin Guan, Richard F. Lance, Sheena M. Feist
Abstract We designed two novel environmental DNA (eDNA) assays for the detection of Ridgway’s Rail ( Rallus obsoletus ), and successfully validated each assay using eDNA samples collected from the species’ known distribution within the United States. These assays add to the suite of tools available for the monitoring of this rare and secretive marsh bird, and may help to further elucidate its movement patterns as well as identify important migration corridors. Observed sensitivity of the assays indicates exceptional performance, with limits of detection at ≤ 8 copies of the target eDNA fragment per reaction. Our publication adds to the growing body of literature supporting eDNA surveys as viable tools for bird monitoring endeavors.
摘要设计了两种检测Ridgway ' s Rail (Rallus obsoletus)的新型环境DNA (eDNA)检测方法，并利用该物种在美国已知分布的eDNA样本成功验证了每种检测方法的有效性。这些分析增加了一套可用的工具来监测这种稀有而神秘的沼泽鸟类，并可能有助于进一步阐明其运动模式以及确定重要的迁徙走廊。观察到的检测灵敏度表明了卓越的性能，每次反应的检测限≤8个目标eDNA片段。我们的出版物增加了越来越多的文献支持eDNA调查作为鸟类监测工作的可行工具。
{"title":"Environmental DNA (eDNA) assays for the detection of Ridgway’s rail (Rallus obsoletus) in the United States","authors":"Xin Guan, Richard F. Lance, Sheena M. Feist","doi":"10.1007/s12686-023-01321-7","DOIUrl":"https://doi.org/10.1007/s12686-023-01321-7","url":null,"abstract":"Abstract We designed two novel environmental DNA (eDNA) assays for the detection of Ridgway’s Rail ( Rallus obsoletus ), and successfully validated each assay using eDNA samples collected from the species’ known distribution within the United States. These assays add to the suite of tools available for the monitoring of this rare and secretive marsh bird, and may help to further elucidate its movement patterns as well as identify important migration corridors. Observed sensitivity of the assays indicates exceptional performance, with limits of detection at ≤ 8 copies of the target eDNA fragment per reaction. Our publication adds to the growing body of literature supporting eDNA surveys as viable tools for bird monitoring endeavors.","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136061217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-16DOI: 10.1007/s12686-023-01323-5
Wenwei Huang, Jiangwei Zhou, Huawei Zhan, Yuying Zhang, Suhan Peng, Wenjun Li, Zusheng Yi, Lin Gui, Chong Han, Qiang Li
{"title":"Isolation and characterization of 32 SNP markers in Spinibarbus hollandi based on restriction site-associated DNA sequencing (RADseq)","authors":"Wenwei Huang, Jiangwei Zhou, Huawei Zhan, Yuying Zhang, Suhan Peng, Wenjun Li, Zusheng Yi, Lin Gui, Chong Han, Qiang Li","doi":"10.1007/s12686-023-01323-5","DOIUrl":"https://doi.org/10.1007/s12686-023-01323-5","url":null,"abstract":"","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135308010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identifying sex and individual from faecal DNA of the Asian elephant using a single multiplex PCR for population monitoring","authors":"Rahul De, Reeta Sharma, Parag Nigam, Amirtharaj Christy Williams, Bilal Habib, Surendra Prakash Goyal","doi":"10.1007/s12686-023-01316-4","DOIUrl":"https://doi.org/10.1007/s12686-023-01316-4","url":null,"abstract":"","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135981105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-22DOI: 10.1007/s12686-023-01315-5
H. Takeshima, Kotomi Yamaura, Natsumi Kuboshima, Miyu Hiramatsu, Chisato Kameya, K. Nohara, K. Sakuma, Satoru N. Chiba, A. Tawa, Nobuaki Suzuki
{"title":"A method for identifying nine tuna and tuna-like species (tribe Thunnini) by using high-resolution melting analysis based on genotyping single nucleotide polymorphisms in mitochondrial DNA","authors":"H. Takeshima, Kotomi Yamaura, Natsumi Kuboshima, Miyu Hiramatsu, Chisato Kameya, K. Nohara, K. Sakuma, Satoru N. Chiba, A. Tawa, Nobuaki Suzuki","doi":"10.1007/s12686-023-01315-5","DOIUrl":"https://doi.org/10.1007/s12686-023-01315-5","url":null,"abstract":"","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45199808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-21DOI: 10.1007/s12686-023-01311-9
M. Tringali, Siobhan B. Gorham, S. Seyoum, C. Puchulutegui, Margaret S. Bass, B. Furman, C. Mallison
{"title":"A PCR assay for the detection of introduced Vallisneria spiralis, V. denseserrulata and their hybrids","authors":"M. Tringali, Siobhan B. Gorham, S. Seyoum, C. Puchulutegui, Margaret S. Bass, B. Furman, C. Mallison","doi":"10.1007/s12686-023-01311-9","DOIUrl":"https://doi.org/10.1007/s12686-023-01311-9","url":null,"abstract":"","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44803371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-21DOI: 10.1007/s12686-023-01312-8
U. S. Leão, L. G. Bueno, A. B. Negreiros, G. R. Silva, R. Maggioni, F. B. Britto, Jose L. R. Sarmento, D. Galvani, F. Diniz
{"title":"Unveiling the demographic background and genetic diversity of Urochloa mosambicensis (Poaceae) through genome-wide identification of simple sequence repeats and molecular marker development","authors":"U. S. Leão, L. G. Bueno, A. B. Negreiros, G. R. Silva, R. Maggioni, F. B. Britto, Jose L. R. Sarmento, D. Galvani, F. Diniz","doi":"10.1007/s12686-023-01312-8","DOIUrl":"https://doi.org/10.1007/s12686-023-01312-8","url":null,"abstract":"","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44721672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-09DOI: 10.1007/s12686-023-01313-7
M. Padrón, M. Milhes, A. Delcamp, E. Guichoux, K. Guizien
{"title":"Development of a set of SNP markers for population genetics of the sea peach (Halocynthia papillosa)","authors":"M. Padrón, M. Milhes, A. Delcamp, E. Guichoux, K. Guizien","doi":"10.1007/s12686-023-01313-7","DOIUrl":"https://doi.org/10.1007/s12686-023-01313-7","url":null,"abstract":"","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47913838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-05DOI: 10.1007/s12686-023-01314-6
Chang-Deuk Park, Keun-Sik Kim, Keun-Yong Kim, Jung Soo Heo, Hong-Shik Oh, Seon-Mi Park, Ju-Duk Yoon
{"title":"Development of the genotyping marker for Reeves’ turtle (Mauremys reevesii) using high-resolution melting (HRM) analysis","authors":"Chang-Deuk Park, Keun-Sik Kim, Keun-Yong Kim, Jung Soo Heo, Hong-Shik Oh, Seon-Mi Park, Ju-Duk Yoon","doi":"10.1007/s12686-023-01314-6","DOIUrl":"https://doi.org/10.1007/s12686-023-01314-6","url":null,"abstract":"","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41763636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-08DOI: 10.1007/s12686-023-01309-3
N. Sastre, A. Mercadé, J. Casellas
{"title":"SNP+ to predict dropout rates in SNP arrays","authors":"N. Sastre, A. Mercadé, J. Casellas","doi":"10.1007/s12686-023-01309-3","DOIUrl":"https://doi.org/10.1007/s12686-023-01309-3","url":null,"abstract":"","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48174522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1007/s12686-023-01307-5
Patrick R. Hutchins, J. J. Giersch, Adam J. Sepulveda, C. Muhlfeld
{"title":"Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly, Lednia tumana, in environmental samples","authors":"Patrick R. Hutchins, J. J. Giersch, Adam J. Sepulveda, C. Muhlfeld","doi":"10.1007/s12686-023-01307-5","DOIUrl":"https://doi.org/10.1007/s12686-023-01307-5","url":null,"abstract":"","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46999202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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