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Effect of Phytohormones on rooting behaviour in air layers of Phoebe cooperiana, an economically important tree species of Arunachal Pradesh, India 植物激素对印度**经济树种菲比(Phoebe cooperiana)气层生根行为的影响
Pub Date : 2021-10-13 DOI: 10.25081/cb.2021.v12.7187
Jasmine Pabin, N. Lyngdoh, T. Mehra, M. B. Devi, Temin Payum
This study reports the response of air layers of Phoebe cooperiana (Common name: Tapil) to different concentrations of rooting hormones in mid hills of Arunachal Pradesh. Ten trees between the ages of 7 and 10 were selected at Boleng village of Siang district, Arunachal Pradesh, India for the experiment. Eight treatments comprising of IBA and NAA at concentrations of 1000ppm, 2000ppm and 3000ppm each, Rootex hormone for semi-hardwood and control were applied to a total of 240 layers during April 2020. After 60 days, rooting percentage, mean number of roots and length of the longest root were recorded. Significant variation (p<0.05) among treatments was observed for all root parameters. Among treatments, the highest rooting percentage and mean a number of roots were obtained in layers treated with NAA 3000ppm (80% and 21.33 respectively). The length of the longest root was the highest in layers treated with NBA 2000ppm (4.23cm). The highest survival percentage of layers two months after transplanting was observed in those treated with NAA 3000ppm (54.16%) and the least in control (23.07%). The technique offers an easy and cheap method of propagation for farmers as well as for government and private agencies to enhance seedling production of the species.
本研究报道了**中山凤梨(Phoebe cooperiana,俗称:Tapil)气层对不同浓度生根激素的响应。在印度**省的博棱村挑选了10棵年龄在7到10岁之间的树进行试验。2020年4月,采用浓度分别为1000ppm、2000ppm和3000ppm的IBA和NAA、半硬木Rootex激素和对照剂8个处理,共240层。60 d后记录生根率、平均根数和最长根长度。各处理间根系参数差异显著(p<0.05)。其中,NAA浓度为3000ppm处理的生根率和平均根数最高,分别为80%和21.33%。在NBA 2000ppm处理下,最长根的长度最高(4.23cm)。NAA 3000ppm处理2个月后成活率最高,为54.16%,对照组最低,为23.07%。该技术为农民以及政府和私人机构提供了一种简单而廉价的繁殖方法,以提高该物种的苗木产量。
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引用次数: 1
Dyeing of cotton fabric by Caesalpinia sappan aqueous extract at different temperatures and mordants 在不同温度和媒染剂条件下,用皂荚水提液对棉织物进行染色
Pub Date : 2021-09-22 DOI: 10.25081/cb.2021.v12.7277
K. Kannathasan, P. Kokila
The colour is one of the most important features in textile industry and customers requirements. Synthetic colours are available at affordable prices due to their bulk production. On the other hand, they pose undesirable taste (in case of foods) and harmful effects to ecosystem. The natural colours when used in textiles do not threat the environments and do not cause any skin allergy, toxicity and other hazards to living things as compared to the synthetic counterparts. In the present study, dyeing experiments were conducted with the aqueous extract of bark chips of Caesalpinia sappan at 60, 80 and100° C and using different mordant treatments. The fastness to washing for most of cotton fabrics showed fair grey scale rating. Among the mordants Alum, CuSo4 and Myrobalan used, the natural mordant myrobalan showed poor fastness properties compared to other two mordants. The staining tests showed that most of fabrics exhibited only slight/completely no pilling in majority of the treatments.
颜色是纺织工业和客户要求中最重要的特征之一。由于它们的批量生产,合成色素以可承受的价格提供。另一方面,它们带来了不良的味道(在食物的情况下)和对生态系统有害的影响。与合成色素相比,用于纺织品的天然色素不会对环境造成威胁,也不会对生物造成任何皮肤过敏、毒性和其他危害。在本研究中,对杉木树皮片的水提液在60、80和100℃下进行了不同媒染剂处理的染色实验。大多数棉织物的耐洗牢度呈中等灰度等级。在明矾、CuSo4和Myrobalan三种媒染剂中,天然媒染剂Myrobalan的牢度性能较差。染色试验表明,大多数织物在大多数处理中只有轻微的起球或完全没有起球。
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引用次数: 3
Biochemical composition, nutritional analysis and antioxidant activity of Buchanania lanzan Spreng fruits 兰赞春果的生化组成、营养分析及抗氧化活性
Pub Date : 2021-09-21 DOI: 10.25081/cb.2021.v12.6852
M. Suryawanshi, Vilas T. Patil, V. Jadhav
Buchanania lanzan Spreng belongs to the family Anacardiaceae. The primary focus of this study was to examine phytochemical, proximate, antioxidant property and GCMS evaluation of unripe and ripen fruit of B. lanzan Spreng. The phytochemical studies showed that alkaloids, phenols, flavones, saponins, coumarins, glycosides and tannins are present in both ripen and unripe fruits. The proximate evaluation confirmed that crude fat (14.5%) and protein (6.37±0.69 g/100g) is high in ripen fruit. In unripe fruit (11.3%) crude fat and protein (4.11%) is less percent. The carotenoid (5.58 ± 0.5 mg/100g) and catalase (0.226±0.074 mg/100g) activity is higher in unripe fruit. The total polyphenol (6.4±0.8 mg/100g) and peroxidase (0.362±0.017 mg/100g) content show greater activity in ripen fruit. In DPPH and FRAP highest activity showed in methanol extract of ripen and unripe fruit than the other solvent. GC-MS evaluation showed many bioactive compounds present in unripe and ripen fruit. It is concluded that nutritional and bioactive ability is high in ripen and unripe fruits of B. lanzan. The fruits of B.lanzan are a good source of nutrition and medicinally important.
布坎南兰山泉属水仙科。本研究的主要目的是研究兰赞春未熟和成熟果实的植物化学、近因性、抗氧化性和GCMS评价。植物化学研究表明,成熟和未成熟的水果中均含有生物碱、酚类、黄酮、皂苷、香豆素、糖苷和单宁。初步评价表明,成熟果实中含有较高的粗脂肪(14.5%)和蛋白质(6.37±0.69 g/100g)。在未成熟的水果(11.3%)中,粗脂肪和蛋白质(4.11%)较少。未成熟果实的类胡萝卜素(5.58±0.5 mg/100g)和过氧化氢酶(0.226±0.074 mg/100g)活性较高。总多酚(6.4±0.8 mg/100g)和过氧化物酶(0.362±0.017 mg/100g)含量在成熟果实中表现出较高的活性。在DPPH和FRAP中,成熟和未成熟果实甲醇提取物的活性最高。气相色谱-质谱分析表明,未成熟和成熟果实中均含有多种活性成分。综上所述,兰赞成熟果实和未成熟果实的营养和生物活性均较高。山核桃的果实是营养和药用的良好来源。
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引用次数: 0
Biosynthesis and characterization of silver nanoparticles using ginger spent and their antibacterial activity 姜末纳米银的生物合成与表征及其抗菌活性
Pub Date : 2021-09-20 DOI: 10.25081/cb.2021.v12.6590
S. Ganesan, P. Mehalingam, G. Selvam
Ginger spent is the byproduct of spice industries that remove the essential oils of ginger (Zingiber officinale) for food industry and medicinal purposes. Ginger is a well known spice used often for seasoning in Indian cuisine. The de-oiled ginger has no specific use mostly goes to waste. Hence, we utilized this industrial waste product in the efficient synthesis of silver nanoparticles with the aid of UV irradiation from a solution of 1mM silver nitrate and spent extract in the ratio 9:1. Immediate colour change from pale yellow to dark brown was noted indicating the rapid synthesis of silver nanoparticles. These nanoparticles were centrifuged, dried and well characterized. UV Vis Spectroscopy, XRD analysis, Zeta potential and SEM analysis was carried out. It was commendable that the size of the nanoparticles fell well within the upper limit of 100nm. Agar well diffusion method was used to screen the antimicrobial activity of the well characterized silver nanoparticles. They were tested against seven pathogenic strains of three gram negative bacteria (Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa) three gram positive bacteria(Bacillus subtilis, Staphylococcus aureus and Streptococcus faecalis) and a fungus (Candida  albicans). It was seen that the zone of inhibition(ZOI) in well plate method  increased on increasing the concentration of silver nanoparticles. Further studies could lead to the application of these silver nanoparticles in medicine.
生姜是香料工业去除生姜精油的副产品,用于食品工业和药用目的。姜是一种众所周知的香料,经常用于印度菜的调味。去油的姜没有什么特殊用途,大部分都被浪费了。因此,我们利用该工业废料,在紫外线照射下,以1mM硝酸银和废提取物为溶液,以9:1的比例高效合成纳米银。颜色立即从淡黄色变为深棕色,表明银纳米颗粒的合成速度很快。对这些纳米颗粒进行离心、干燥和表征。进行了紫外可见光谱分析、XRD分析、Zeta电位分析和SEM分析。值得称道的是,纳米颗粒的尺寸落在了100nm的上限之内。采用琼脂孔扩散法对表征良好的纳米银进行抑菌活性筛选。它们对三种革兰氏阴性菌(大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌)、三种革兰氏阳性菌(枯草芽孢杆菌、金黄色葡萄球菌和粪链球菌)和一种真菌(白色念珠菌)的七种致病菌株进行了检测。结果表明,孔板法的抑制带(ZOI)随着银纳米粒子浓度的增加而增加。进一步的研究可能会导致这些银纳米颗粒在医学上的应用。
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引用次数: 1
Development of wound dressing film using methanolic extracts of freshwater microalgae and determining its wound healing ability 淡水微藻甲醇提取物创面敷料的研制及其创面愈合能力的测定
Pub Date : 2021-09-20 DOI: 10.25081/cb.2021.v12.6986
S. Soundararajan, R. Raja, S. V. Chitthan, S. Prasad, N. Thajuddin
Wound healing is a complex process in which bacterial infection is a major cause for delayed wound healing. Occurrence of drug resistance among bacterial pathogens led to discovery of new antimicrobial agents from new sources. The present study aimed to identify microalgal metabolites with antibacterial activity and to develop a wound dressing film with their potential healing activity. Microalgal samples were collected from three different freshwater habitats, isolated, made them pure cultures and physico-chemical properties of water samples from the respective sampling sites were analzed. Among the three microalgal isolates,  Chlorella sp. NRMC-F-0350 showed antibacterial activity against clinical isolates of  E. coli, Klebsiella pneumoniae and Staphylococcus aureus. Additionally, functional groups present in Chlorella sp. NRMC-F-0350 were identified using FTIR and GC-MS analysis. Several antibacterial compounds viz. benzoxazole, 1,2 Benzene dicarboxylic acid, sistosterol, 9-Octadecanoic acid, eicosane and hexadecane were identified. Wound dressing films were developed and showed evident antibacterial as well as significant wound healing activity (84.5%). Therefore, the developed films can be used as a potential wound dressing material. 
伤口愈合是一个复杂的过程,其中细菌感染是导致伤口愈合延迟的主要原因。细菌病原菌耐药的发生导致从新的来源发现新的抗微生物药物。本研究旨在鉴定具有抗菌活性的微藻代谢物,并开发具有潜在愈合活性的伤口敷料膜。从3个不同的淡水生境采集微藻样品,进行分离、纯培养,并对各采样点水样的理化性质进行分析。3株微藻中,小球藻sp. NRMC-F-0350对临床分离的大肠杆菌、肺炎克雷伯菌和金黄色葡萄球菌均有抑菌活性。此外,利用FTIR和GC-MS分析鉴定了小球藻NRMC-F-0350中存在的官能团。鉴定出苯并恶唑、1,2苯二甲酸、姐妹甾醇、9-十八烷酸、二十烷和十六烷等抗菌化合物。创面敷料膜具有明显的抗菌作用和显著的创面愈合活性(84.5%)。因此,显影膜可作为一种潜在的伤口敷料。
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引用次数: 0
A comprehensive evaluation of pectinase, pectinmethylesterase and pectolyase activity 果胶酶、果胶甲基酯酶和果胶酶活性的综合评价
Pub Date : 2021-09-14 DOI: 10.25081/cb.2021.v12.6579
Karishma Rajbhar, Himanshu G. Dawda, U. Mukundan
Pectin polysaccharide has galacturonic acid with linear chains of α-(1–4)-linked D- galacturonic acid.  Rhamnogalacturonan I pectins (RG-I) shows the existence of the repeating disaccharide 4-α-D-galacturonic acid-(1,2)-α-L- rhamnose, which acts as a backbone. Chiefly, D-galactose, L-arabinose, and D-xylose are the sugars types and its proportions of neutral sugars are varied according to the origin of pectin. Pectinase, pectinmethylesterase, and pectolyase enzymes have important applications in food, textile and agricultural industries. These enzymes play an important role in the breakdown of the central part of the plant cell wall. Pectin forms the center part of the plant cell wall. Pectins are termed as structural polysaccharide that has integrity for the steadiness of the plant cell wall. Citrate buffer of molarity 0.1 utilized to verify optimal pH along with temperature, for standardising enzyme activity of pectinase, pectolyase, and pectinmethylesterase by the dinitrosalicylic acid reagent method. Confirmatory check of enzyme’s activity was performed on plant leaves dried particles.  Impact of catechin presence in enzyme reaction was too studied. Results delve into degradation of the plant polysaccharide by applying these enzymes.  An increase in the monosaccharide galacturonic acid quantity was also significant. The highest release of the polyphenols was found due to pectolyase followed by pectinmethylesterase and pectinase. Pectinmethylesterase effect showed the maximum release of the flavonoids followed by pectinase and pectolyase which was remarkable.
果胶多糖含有半乳糖醛酸,具有α-(1-4)-连接D-半乳糖醛酸的线性链。鼠李糖半乳糖酸I果胶(RG-I)中含有重复双糖4-α- d-半乳糖酸-(1,2)-α- l -鼠李糖,作为主干结构。主要是d -半乳糖、l -阿拉伯糖和d -木糖,其中性糖的比例根据果胶的来源而变化。果胶酶、果胶甲基酯酶和果胶水解酶在食品、纺织和农业工业中有着重要的应用。这些酶在植物细胞壁中心部分的分解中起着重要作用。果胶形成植物细胞壁的中心部分。果胶被称为结构多糖,具有植物细胞壁稳定性的完整性。柠檬酸缓冲液的摩尔浓度为0.1,用二硝基水杨酸试剂法对果胶酶、果胶水解酶和果胶甲基酯酶的酶活性进行了最佳pH随温度的验证。对植物叶片干燥颗粒进行了酶活性的验证性检测。研究了儿茶素的存在对酶反应的影响。结果研究了这些酶对植物多糖的降解作用。单糖半乳糖醛酸的含量也显著增加。果胶酶释放多酚量最大,其次是果胶甲基酯酶和果胶酶。果胶甲基酯酶效应表现为黄酮类化合物释放量最大,果胶酶和果胶酶释放量次之。
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引用次数: 0
Genetic status of Centella asiatica (L.) Urb. (Indian pennywort): A review 积雪草的遗传状况市区。(印度苦艾草):一篇评论
Pub Date : 2021-09-10 DOI: 10.25081/cb.2021.v12.6971
Sakthipriya Mathavaraj, K. Sabu
In recent years, demand for medicinal plants increased due to the rise in attraction towards herbal products which are safer compared to modern drugs. Centella asiatica (L.) Urb is known as an important medicinal plant in herbal medicinal systems. It also used as an active ingredient for many products in the pharmaceutical and cosmetic industry. So far, review on this plant concerns mainly on medicinal, cosmetology and photochemical works reported. This review presents the genetic studies conducted in this herb along with a mention on conservation. Since documenting and studying genetic variation and its composition has an important connection for the understanding of evolution and improving the conservation of this species.
近年来,由于对草药产品的吸引力增加,对药用植物的需求增加,因为草药产品比现代药物更安全。积雪草(Centella asiatica)乌兰是中草药系统中一种重要的药用植物。它也被用作制药和化妆品行业许多产品的活性成分。目前,国内外对该植物的研究主要集中在药用、美容和光化学方面。本文综述了对这种草本植物进行的遗传研究,并提出了保护的建议。因为记录和研究遗传变异及其组成对了解该物种的进化和改善其保护具有重要的联系。
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引用次数: 2
Impact of tannery effluent on Sorghum bicolor (L.) Moench 制革废水对高粱双色菌的影响Moench
Pub Date : 2021-08-09 DOI: 10.25081/cb.2021.v12.7148
V. Sangeetha, P.S. Sharavannan
The present study is aimed to investigate the effect of tannery effluent on different varieties of Sorghum bicolor. The disparate concentrations of tannery effluent viz., 5, 25, 50, 75 and 100% were tested for its impacts on six varieties of Sorghum bicolor (L.) Moench (TNAU CO 5, TNAU CO 30, CO (S) 28, BSR 1, K Tall and Paiyur 1). The experimentation was carried out in a completely randomized design (CRD) with five replications. The impact of tannery effluent on the germination percentage, shoot length (cm), root length (cm), seedling weight (g), vigour index, tolerance index and phytotoxicity were recorded. The outcome revealed that the parameters escalated in 5% effluent concentration and declined after 25% concentration of tannery effluent.
本研究旨在探讨制革废水对不同品种双色高粱的影响。研究了不同浓度的制革废水(5、25、50、75和100%)对6个高粱品种的影响。Moench (TNAU CO 5、TNAU CO 30、CO (S) 28、BSR 1、K Tall和Paiyur 1)。试验采用完全随机设计(CRD), 5个重复。记录制革废水对发芽率、芽长、根长、苗重、活力指数、耐受性指数和植物毒性的影响。结果表明,当制革废水浓度为5%时,这些参数升高,当制革废水浓度为25%时,这些参数下降。
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引用次数: 0
Density, diversity and community composition of trees in tropical thorn forest, peninsular India 印度半岛热带刺林树木密度、多样性和群落组成
Pub Date : 2021-08-07 DOI: 10.25081/cb.2021.v12.7138
J. Evitex-Izayas, M. Udayakumar
Tropical thorn forests (TTFs) are characterized by the presence of small and thorny trees which usually shed their leaves in dry season. A quantitative phytosociological study was conducted in Uthumalai Reserve Forest of Peninsular India to record density, species richness, diversity and population structure of trees. Diameter of all free standing trees ≥1 cm diameter at breast height (DBH, cm) was measured at 1.37 m above the ground. A sum of 4135 trees ≥1cm DBH recorded from one hectare study plot. With 2272 (54.94%) individuals Dalbergia spinosa dominated the study plot followed by Commiphora berryi (484, 11.70%), Grewia flavescens (259, 6.26%), Dichrostachys cinerea (206, 4.98%) and Anogeissus pendula (171, 4.14%). In total, 26 species belonged to 19 genera and 15 families found in one ha study plot. The family Mimosaceae had a large number of species followed by Apocynaceae, Capparidaceae, Tiliaceae, Rhamnaceae. Rubiaceae (each 2 species). Stand basal area of tree community recorded as 15.238 m2 ha-1. Commiphora berryi constituted 50.80% (7.74 m2 ha-1) of stand basal area followed by Dalbergia spinosa (19.43%, 2.96 m2 ha-1). The forest showed a reverse J shaped population structure. Shannon diversity (H), equitability (H’) and Simpson’s dominance (D) indices of study area recorded as 1.76, 0.54 and 0.335, respectively. Dalbergia spinosa, Commiphora berryi, Grewia flavescens, Dichrostachys cinerea and Anogeissus pendula topped the species important value index with 87.80, 73.53, 20, 19.79, 17.43 scores, respectively. In family important value index, Papilionaceae topped the list followed by Burseraceae and Mimosaceae with scores 129.32, 74.23 and 34.43, respectively. The study area endowed with a moderate species richness and diversity, and acts as one of the homes for an IUCN’s vulnerable tree species.
热带荆棘林(ttf)的特点是存在小而多刺的树木,通常在旱季落叶。对印度半岛乌图马莱保护区的树木密度、物种丰富度、多样性和种群结构进行了定量的植物社会学研究。在离地1.37 m处测量所有胸径≥1cm的独立立木的直径(DBH, cm)。每公顷研究地块共记录≥1cm胸径的树木4135棵。黄檀(Dalbergia spinosa)以2272只(54.94%)居多,其次是金粉虱(commiora berryi)(484只,11.70%)、黄灰灰虱(Grewia flavescens)(259只,6.26%)、灰灰灰虱(Dichrostachys cinerea)(206只,4.98%)和钟鳞灰虱(Anogeissus pendula)(171只,4.14%)。1公顷样地共发现15科19属26种。含羞草科的种类较多,其次是夹竹桃科、麻瓜科、铁力科、鼠李科。茜草科(各2种)。乔木群落林分基面积为15.238 m2 ha-1。红木占林分基础面积的50.80% (7.74 m2 ha-1),其次是白檀(19.43%,2.96 m2 ha-1)。种群结构呈倒J型。研究区香农多样性指数(H)、公平度指数(H’)和辛普森优势度指数(D)分别为1.76、0.54和0.335。黄檀(Dalbergia spinosa)、红木(commihora berryi)、黄灰(Grewia flavescens)、灰灰(Dichrostachys cinerea)和苦参(Anogeissus pendula)的重要价值指数分别为87.80、73.53、20、19.79和17.43分,居首位。科重要价值指数中,凤蝶科以129.32分、74.23分、34.43分居首位,其次为麻丝科和含羞草科。研究区具有中等的物种丰富度和多样性,是世界自然保护联盟脆弱树种的家园之一。
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引用次数: 1
Anatomical studies on the leaf and stem of Tinospora formanii Udayan & Pradeep (Menispermaceae), an endemic species to Southern Western Ghats, Kerala, India 印度喀拉拉邦西南高止山脉特有种Tinospora formanii Udayan & Pradeep (menispermacae)叶和茎的解剖学研究
Pub Date : 2021-07-23 DOI: 10.25081/CB.2021.V12.6455
Patturaj Raj, S. N. Begum, K. Ravikumar, P. Dharmapal, P. Udayan
The anatomical studies on leaf and stem of T. formanii Udayan & Pradeep an endemic species to southern Western Ghats, Kerala, India was carried out focusing on its macroscopic, microscopic, maceration along with organoleptic evaluation. Distinguishing characters of the stem revealed the presence of calcium oxalate crystals, simple and compound starch grains and pitted lignified fibers. Leaf anatomy showed the anomocytic and paracytic stomata, pitted lignified fibers, spiral vessels, non-glandular small trichomes, C or half-moon shaped vascular bundle, surrounded with sclerenchymatous tissues and rosette and prism shaped calcium oxalate crystals. Whereas, maceration studies revealed the presence of spiral and scalar form vessel, fibers, calcium oxalate crystals, simple starch grains. These anatomical studies are vital in the present-day trade scenario not only helpful in the proper identification of the genuine materials in use but also to distinguish different species of Tinospora, where the stem and leaf are often admixed with other species of Tinospora in the crude drug markets.
本文对印度喀拉拉邦西高止山脉南部特有物种乌达扬&普拉迪普(T. manoii Udayan & Pradeep)的叶和茎进行了宏观、微观、浸渍和感官评价的解剖研究。茎部的特征显示草酸钙晶体、简单和复合淀粉粒和麻点木质化纤维。叶片解剖显示无规则细胞和副细胞气孔,木质素纤维,螺旋状血管,非腺状小毛状体,C形或半月形维管束,周围有厚壁组织和玫瑰状和棱镜状草酸钙晶体。然而,浸渍研究显示螺旋形和标量形血管、纤维、草酸钙晶体、简单淀粉颗粒的存在。这些解剖研究在当今的贸易情况下是至关重要的,不仅有助于正确识别使用的真正材料,而且还有助于区分不同种类的Tinospora,其中茎和叶经常与其他种类的Tinospora在生药市场上混合。
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引用次数: 0
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Current Botany
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