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LC-MS/MS Targeted Amino Acid Profiling of Edible Beetle Anomala Sp. and its Host Plants 食用甲虫 Anomala Sp.及其寄主植物的 LC-MS/MS 氨基酸靶向分析
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.23
N. Muni, Prveen Prveen, Y. Rajashekar, J. Chakravorty
Anomala sp. is a green coleopteran beetle that feeds on the leaves of various fruit trees. It is one of the most preferred and abundantly found edible insects in Arunachal Pradesh, India. People from different countries consume it due to its nutritional and therapeutic value, both in cooked and uncooked form. This study aimed to assess the amino acid content in this edible insect and its host plant, which is still lagging. The method involves targeted metabolomic analysis using LC–MS/MS, producing reliable and reproducible data for quantifying free amino acids using deuterated internal standards without derivatization. Results showed that 0.57% and 0.36% of essential amino acids contributed to the total amino acids content in beetle and host plant’s leaf, respectively, which does not meet the FAO requirement. Still, it has a higher content than related edible scarab beetle analyzed by other techniques. Amino acid content is significantly higher in edible beetle than in the host plant’s leaf (P< 0.001).
Anomala sp. 是一种绿色鞘翅目甲虫,以各种果树的叶子为食。它是印度阿鲁纳恰尔邦最受欢迎和最常见的食用昆虫之一。由于其营养和食疗价值,不同国家的人们都食用它,无论是煮熟的还是未煮熟的。这项研究旨在评估这种食用昆虫及其寄主植物中的氨基酸含量,目前这项工作还很滞后。该方法采用 LC-MS/MS 进行有针对性的代谢组学分析,使用氘代内标对游离氨基酸进行定量,无需衍生,数据可靠,重现性好。结果表明,甲虫和寄主植物叶片中的必需氨基酸分别占总氨基酸含量的0.57%和0.36%,不符合粮农组织的要求。尽管如此,其含量仍高于其他技术分析的相关食用猩甲虫。食用甲虫的氨基酸含量明显高于寄主植物叶片(P< 0.001)。
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引用次数: 0
IoT implemented Osmotic Dehydrator 物联网实施的渗透脱水机
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.25
Lesly C. Flores- Mendoza, Estrellita M. Calle- Berru, Manuel Jesús Sánchez Chero
Osmotic dehydration is a dehydration technique that allows the preservation of the organoleptic characteristics of the food and its nutritional properties compared to other preservation methods, such as hot air drying or solar drying. Studies on this dehydration process are usually carried out on a laboratory scale and with the constant presence of the evaluator, interrupting the process for sampling. That is why the main objective of this research was to build an automated osmotic dehydration equipment implementing the Internet of Things (IoT). The measurable factors involved in the process were determined, and the necessary sensors and actuators were chosen along with the best IoT alternative for the process. A prototype was built, which allows for controlling the agitation of the osmotic solution, temperature control, and remote monitoring of concentration and temperature variables. The equipment was tested by evaluating its performance in the dehydration of melon and apple, where the ANOVA tests demonstrated the significance (p < 0.05) of the factors chosen as part of the equipment design and their interaction with the process. In the apple samples, a weight loss of up to 44.007% and a water loss of 53.234% were obtained. For melon, the process showed greater efficiency in dehydration, with values of 75.259% for weight loss and 75.979% for water loss.
渗透脱水是一种脱水技术,与其他保存方法(如热风干燥或太阳能干燥)相比,它能保存食品的感官特征及其营养特性。对这种脱水工艺的研究通常是在实验室进行的,评估人员必须始终在场,并在取样时中断整个过程。因此,本研究的主要目标是建立一个采用物联网(IoT)技术的自动渗透脱水设备。研究人员确定了过程中涉及的可测量因素,选择了必要的传感器和执行器,并为过程选择了最佳的物联网替代方案。我们制作了一个原型,可以控制渗透溶液的搅拌、温度控制以及浓度和温度变量的远程监控。通过评估甜瓜和苹果的脱水性能,对设备进行了测试,方差分析测试表明,作为设备设计一部分而选择的因素及其与工艺的交互作用具有显著性(p < 0.05)。苹果样品的重量损失高达 44.007%,水分损失为 53.234%。甜瓜的脱水效率更高,重量损失率为 75.259%,水分损失率为 75.979%。
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引用次数: 0
Nutritional Profiling, Antioxidant Potential and Collagen Building Properties of Lacto-Fermented, Microencapsulated Guava Juice Powder 乳酸发酵微胶囊番石榴汁粉的营养分析、抗氧化潜力和胶原蛋白生成特性
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.16
Ratna Upadhyay, Siva Ramamoorthy
Fermented products consist beneficial probiotics, which makes these products render improved nutritional properties and health effects. There is a need for research to explore and establish the use of commonly existing fruits in fermented forms for application in skincare and as anti aging solutions.This study has been conducted to establish collagen building and antioxidant potential of fermented guava fruit juice powder.The human dermal fibroblast cells were used to determine the collagen building potential while chemical enzymatic assays were used to evaluate antioxidant properties of fermented guava juice powder (FGJP) . The antioxidant activity evaluated by the ability to scavenge DPPH radicals showed very high anti-oxidative capacity (IC50 = 0.015 mg/ml) comparable to ascorbic acid (IC50 = 0.018 mg/ml). This antioxidant potential can be attributed to the presence of various phytonutrients, primarily flavonoid (quercetin equivalent) at a concentration of 38 mcg/mg of the powder. The Collagen building potential was evaluated on the normal human fibroblast cell line . The levels of Collagen IV involved in skin cell migration, regeneration, and wound healing were estimated. FGJP imparted proliferation ability in NHDF cells at the concentrations of 0.025, 0.05, and 0.1mg/ml,which is 31% collagen synthesis against untreated cell control. This explains that fermented guava juice powder has a collagen building potential along with antioxidant properties. Such product would be helpful in providing a plant based anti aging solutions especially for vegan consumers.The optimized fermented guava juice powder has a shelf life of 45 days when stored at refrigerated conditions with a potential for combating oxidative stress and supporting in alleviating the aging symptoms like fine-line, wrinkle through collagen building.
发酵产品中含有有益的益生菌,使这些产品具有更好的营养特性和保健效果。本研究旨在确定发酵番石榴果汁粉的胶原蛋白生成和抗氧化潜力。研究人员使用人类真皮成纤维细胞来确定胶原蛋白生成潜力,并使用化学酶测定法来评估发酵番石榴果汁粉(FGJP)的抗氧化特性。通过清除 DPPH 自由基的能力来评估抗氧化活性,结果显示其抗氧化能力非常高(IC50 = 0.015 mg/ml),与抗坏血酸(IC50 = 0.018 mg/ml)相当。这种抗氧化潜力可归因于各种植物营养素的存在,主要是黄酮类化合物(槲皮素当量),浓度为 38 微克/毫克粉末。在正常人成纤维细胞系上对胶原蛋白的生成潜力进行了评估。评估了参与皮肤细胞迁移、再生和伤口愈合的胶原蛋白 IV 的水平。浓度分别为 0.025、0.05 和 0.1 毫克/毫升时,番石榴果粉能赋予 NHDF 细胞增殖能力,与未处理的细胞对照相比,胶原蛋白的合成量增加了 31%。这说明发酵番石榴果汁粉具有增加胶原蛋白的潜力和抗氧化特性。经过优化的发酵番石榴果汁粉在冷藏条件下保存的保质期为 45 天,具有对抗氧化应激的潜力,并能通过胶原蛋白的生成缓解细纹、皱纹等衰老症状。
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引用次数: 0
Extraction and Industrial Applications of Macro Molecules: A Review 大分子的提取和工业应用:综述
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.02
Abiodun Famakinwa, Jumoke Ilo, O. Olubi, O. Oguntibeju, J. Wyk, A. Obilana
Macromolecules, large molecules composed of repeating subunits called monomers, play a crucial role in living organisms, performing diverse functions such as energy storage, structural support, information storage and transfer, and catalysis of chemical reactions. Carbohydrates, lipids, proteins, and nucleic acids are the four major classes of biological macromolecules. Extracting macromolecules from natural resources is critical in developing analytical processes and subsequent fortified products. Public awareness has grown due to using natural resources for environmental preservation and sustainable development. Extraction might be the first step in developing analytical methods and give room to product development. However, conventional techniques use organic solvents like acetone, ethanol, methanol, and ethyl acetate along with heated or boiling water. As a result, high temperatures and lengthy extraction times are produced when procedures like maceration, percolation, and solvent extraction are utilized. Due to these drawbacks, other extraction techniques have recently started to replace these conventional methods. These conventional procedures frequently entail using an energy source to hasten the transfer of the macromolecules compounds for further processing. This paper explores emerging techniques, such as pulse electric field-assisted extraction, Ionic liquid-based extraction, Subcritical water extraction, pressurized liquid extraction, Enzyme-assisted extraction, supercritical fluid extraction, ultrasonication-assisted extraction, and microwave-assisted extraction. The extraction of macromolecules for fortification purposes offers significant health and commercial benefits, addressing nutritional deficits and malnutrition. By understanding each macronutrient's specific benefits and purposes, effective fortification strategies can be developed to maintain a healthy body.
大分子是由称为单体的重复亚基组成的大分子,在生物体内发挥着至关重要的作用,具有能量储存、结构支撑、信息储存和传递以及催化化学反应等多种功能。碳水化合物、脂类、蛋白质和核酸是生物大分子的四大类。从自然资源中提取大分子对于开发分析过程和后续强化产品至关重要。公众对利用自然资源保护环境和实现可持续发展的认识不断提高。提取可能是开发分析方法的第一步,也为产品开发提供了空间。然而,传统技术使用丙酮、乙醇、甲醇和乙酸乙酯等有机溶剂以及加热或沸腾的水。因此,在使用浸渍、渗滤和溶剂萃取等程序时,会产生高温和较长的萃取时间。由于这些缺点,其他萃取技术最近开始取代这些传统方法。这些传统方法通常需要使用能源来加速大分子化合物的转移,以便进一步加工。本文探讨了脉冲电场辅助萃取、离子液体萃取、亚临界水萃取、加压液体萃取、酶辅助萃取、超临界流体萃取、超声辅助萃取和微波辅助萃取等新兴技术。萃取大分子营养素用于营养强化,可为健康和商业带来巨大利益,解决营养缺乏和营养不良问题。通过了解每种宏量营养素的具体益处和用途,可以制定有效的强化策略,以保持健康的身体。
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引用次数: 0
Quarg Cheese: The Impact of Fat Content Change on its Microstructure, Characterization, Rheology, and Textural Properties Quarg 奶酪:脂肪含量变化对其微观结构、表征、流变学和纹理特性的影响
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.12
Shubhendra Singh, Anil Kumar Chauhan, Aparnna V. P, Ravi Prakash, P. Maiti, Rajeev Ranjan, Pallawi Joshi
This study aimed to investigate the impact of different levels of fat content on the microstructure, rheology, and textural characteristics of Quarg cheese. Quarg is a soft unripened cheese variety made by the addition of mesophilic starter cultures (Lactococcus lactis subsp. lactis, Leuconostoc citrovorum) and a trace of rennet. The fat levels of cheese were; 6.0 ± 0.01 (QC1), 3.5 ± 0.03 (QC2), 1.5 ± 0.03 (QC3), and 0.5 ± 0.02 (QC4). Statistically significant changes (p< 0.05) were observed in the values of textural parameters. Furthermore, rheological investigations indicated a positive correlation between the reduction in fat percentages and an increase in the hardness of the cheese. The characterization of the sample was conducted using Fourier Transform Infrared (FTIR) and Ultraviolet-Visible (UV-Vis) spectrophotometric techniques. The microstructure of quarg cheese was analyzed using fluorescence microscopy and helped depict the distribution of fat and protein in the cheese matrix. Sensorial properties were superior in cheese samples with higher fat percentages as a virtue of fat content.
这项研究旨在调查不同水平的脂肪含量对夸尔格奶酪的微观结构、流变学和纹理特征的影响。Quarg 是一种未熟的软质奶酪,是通过添加嗜中性起始培养物(乳酸乳球菌亚种、柠檬白念珠菌)和微量凝乳酶制成的。奶酪的脂肪含量分别为:6.0 ± 0.01(QC1)、3.5 ± 0.03(QC2)、1.5 ± 0.03(QC3)和 0.5 ± 0.02(QC4)。纹理参数值出现了明显的统计学变化(p< 0.05)。此外,流变学研究表明,脂肪百分比的降低与奶酪硬度的增加之间存在正相关。使用傅立叶变换红外(FTIR)和紫外-可见(UV-Vis)分光光度法对样品进行了表征。使用荧光显微镜分析了夸尔格奶酪的微观结构,有助于描述脂肪和蛋白质在奶酪基质中的分布。由于脂肪含量的关系,脂肪百分比较高的奶酪样品的感官特性更佳。
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引用次数: 0
Formulation, Nutritional and Sensory Evaluation of Mocaf (Modified Cassava Flour) Noodles with Latoh (Caulerpa lentillifera) Addition 添加 Latoh(扁豆)的改良木薯粉面条的配方、营养和感官评估
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.08
Sri Budi Wahjuningsih, Dian Anggraeni, Z. D. Siqhny, Agus Triputranto, Dahlia Elianarni, L. Purwitasari, M. N. Azkia
Instant noodles gained widespread popularity, resulting in a surge in global wheat consumption. However, concerns about the health implications of wheat-based products spurred the need for alternative ingredients in noodle production. The substitution of wheat flour with local resources, such as mocaf flour, and the enrichment of noodles with latoh (Caulerpa lentillifera), provided an opportunity to reduce wheat imports while enhancing the quality and potential health benefits of noodles. This study aimed to determine the optimal formulation, assess the characteristics, and evaluate the functional properties of wheat noodles that were substituted with mocaf flour and supplemented with latoh. The formulation of these noodles was conducted using design expert. The research encompassed a comprehensive analysis of physical, chemical, and organoleptic properties. The presence of latoh, in terms of chemical characteristics, increased the water and protein content in the noodles. On the physical side, it increased the final viscosity, setback, peak time, and elongation, as well as reduced the peak viscosity and breakdown. Meanwhile, the presence of mocaf increased carbohydrate content, peak viscosity, and breakdown value. The variations in noodle formulas only affected consumer acceptance of the appearance of the noodles; the noodle formula 99:1 (Mocaf:Latoh) had the most preferred appearance. Based on the sensory test parameters, the elongation value, and the tensile strength, noodles with optimal formulas were obtained, namely mocaf 63%, wheat 36% and latoh 1%. The optimum formula was similar to sample 10 (60:39:1) which exhibited characteristics such as a bright color, a rather smooth appearance with no cracks, a very soft mouthfeel, a bit springiness and elasticity, and a slightly non-sticky texture to the teeth without a fishy aroma. The essential and non-essential amino acids with the highest concentration contained in the Mocaf Noodles’ optimum formula were glutamate and leucine. Mocaf noodles with added latoh had low fat content but high carbohydrates and were rich in essential amino acids compared to other noodles. Additionally, they possessed sensory characteristics that were deemed acceptable by the panelists.
方便面广受欢迎,导致全球小麦消费量激增。然而,由于人们担心小麦产品会影响健康,因此需要在面条生产中使用替代配料。用当地资源(如莫卡夫面粉)替代小麦粉,并在面条中添加拉托(Caulerpa lentillifera),为减少小麦进口,同时提高面条质量和潜在健康益处提供了机会。本研究旨在确定用莫卡夫面粉替代小麦并添加拉托的面条的最佳配方、评估其特点和功能特性。这些面条的配方是通过专家设计进行的。研究包括对物理、化学和感官特性的全面分析。从化学特性来看,拉托的存在增加了面条中的水分和蛋白质含量。在物理特性方面,它增加了最终粘度、回弹率、峰值时间和伸长率,并降低了峰值粘度和分解率。同时,mocaf 的存在增加了碳水化合物含量、峰值粘度和分解值。面条配方的变化只影响消费者对面条外观的接受程度;面条配方 99:1(Mocaf:Latoh)的面条外观最受欢迎。根据感官测试参数、伸长值和拉伸强度,得到了最佳配方的面条,即莫卡夫占 63%,小麦占 36%,拉托占 1%。最佳配方与样品 10(60:39:1)相似,其特点是色泽鲜艳,外观光滑无裂纹,口感非常柔软,有一定的弹力和弹性,吃起来略微不粘牙,没有腥味。莫卡夫面条最佳配方中含量最高的必需氨基酸和非必需氨基酸是谷氨酸和亮氨酸。与其他面条相比,添加了拉多的莫卡夫面条脂肪含量低,但碳水化合物含量高,且含有丰富的必需氨基酸。此外,这些面条还具有小组成员认为可以接受的感官特征。
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引用次数: 0
Increase of Anthocyanin, Vitamin C, and Flavonoid Content in Red Lettuce (Lactuca sativa L.) using Alternating Magnetic Field Exposure 利用交变磁场提高红莴苣(Lactuca sativa L.)中的花青素、维生素 C 和类黄酮含量
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.19
Mokhamad Tirono, Agus Mulyono
Red lettuce (Lactuca sativa L.) is widely consumed for its health benefits. With the a growing global population and limited land resources, there is a need to increase the nutritional content and bioactive compounds of vegetables or fruits. This research aims to increase the growth, productivity, and the content of anthocyanin, vitamin C and flavonoids in red lettuce leaves through exposure to magnetic fields during seeding. The research utilized red lettuce seeds as the research sample. Exposure was carried out using an alternating magnetic field (MF) with an effective magnetic flux density (MFD) of 0.4 mT, a frequency range of 25-125 Hz, and a dailyexposure time of 15 minutes. The treatment of each sample group was repeated for five days. The results showed that exposure to a MF with a frequency of 75 Hz resulted in optimum growth and weight, an increase of 65.15% and 60.18%, respectively. The optimum anthocyanin, vitamin C, and flavonoid content were obtained at an exposure to a frequency of 100 Hz, an increase of 22.52%, 40.70%, and 32.50%, respectively. Exposure to seeds uses an alternating MF; frequency is an important parameter for producing optimum enhancement.
红莴苣(Lactuca sativa L.)因其健康益处而被广泛食用。随着全球人口的增长和土地资源的有限,有必要增加蔬菜或水果的营养成分和生物活性化合物。本研究旨在通过在播种过程中暴露于磁场来提高红莴苣叶片的生长、产量以及花青素、维生素 C 和类黄酮的含量。研究采用红莴苣种子作为研究样本。使用有效磁通密度(MFD)为 0.4 mT、频率范围为 25-125 Hz、每天暴露时间为 15 分钟的交变磁场(MF)进行暴露。每个样本组重复处理五天。结果表明,暴露于频率为 75 赫兹的中频辐射下,鱼类的生长和体重均达到最佳状态,分别增加了 65.15%和 60.18%。频率为 100 赫兹时,花青素、维生素 C 和类黄酮含量最佳,分别增加了 22.52%、40.70% 和 32.50%。对种子的曝晒采用交变中频;频率是产生最佳增强效果的重要参数。
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引用次数: 0
Investigating the Behaviour and Practices of Household Food Waste Disposal of Consumers in the KwaDukuza Municipality, South Africa 调查南非夸杜库扎市消费者处理家庭食物垃圾的行为和做法
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.27
Imana Pal, A. Naicker, Heleen Grobbelaar
Food waste poses a threat to both food security as well as the long-term sustainability of a country’s food system. In South Africa, the expense of post-consumer food waste (mostly from households) is estimated at roughly US$2.7 billion annually, or 0.7% of South Africa's annual GDP. It is unfortunate that so much food is wasted in a country where 26% of people regularly experience hunger and another 28.3% are at risk of becoming hungry at a household level. This study aimed to investigate household food waste practices and behaviours in the KwaDukuza community using a consumer survey. The survey was conducted on 190 households which were randomly selected in KwaDukuza municipality and administered with the structured questionnaire. The study's findings showed that 75% of the households reported doing weekly food shopping, and 87.4 % said they shopped primarily at supermarkets. Bread accounted for the largest share of food waste (28.9%), followed by vegetables and salads (24.7%), and in general, consumers experienced difficulty in making sense of expiry dates. However, many households (67.9%) acknowledged feeling guilty about food waste, and many more (34.2%) said they would waste less if they were informed about the adverse effects of food waste on the environment and economy. The comprehensive information gathered from the food waste survey would contribute to a better understanding of consumers' behaviour and practices regarding the disposal of household food waste, allowing for the development of appropriate food literacy tools to reduce household food waste.
食物浪费对粮食安全和国家粮食系统的长期可持续性都构成了威胁。在南非,消费后的食物浪费(主要来自家庭)估计每年约为 27 亿美元,占南非年 GDP 的 0.7%。令人遗憾的是,在南非,有 26% 的人经常挨饿,另有 28.3% 的家庭面临挨饿的风险,而浪费的食物却如此之多。这项研究旨在通过一项消费者调查,调查夸杜库扎社区家庭浪费食物的做法和行为。调查在夸杜库扎市随机抽取了 190 户家庭,并发放了结构化问卷。调查结果显示,75%的家庭表示每周都会进行食品购物,87.4%的家庭表示他们主要在超市购物。在食物浪费中,面包所占的比例最大(28.9%),其次是蔬菜和沙拉(24.7%)。然而,许多家庭(67.9%)承认对浪费食物感到内疚,更多家庭(34.2%)表示,如果他们了解到浪费食物对环境和经济的不利影响,他们会减少浪费食物。从食物浪费调查中收集到的全面信息将有助于更好地了解消费者处理家庭食物浪费的行为和做法,从而开发适当的食物知识工具,减少家庭食物浪费。
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引用次数: 0
Development of Functional Milk-based Smoothie by Incorporating Horse Gram Extract 通过添加马齿苋提取物开发功能性奶基冰沙
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.26
Priyanka Panda, Sunil Meena, Kamlesh Meena, Dinesh Chandra Rai, Shankar Bunkar, P. Gautam
In the last few decades, the demand for fruits and vegetables and mixed beverages such as juices and smoothies increased rapidly, which made it one of the highest-growing segments of the food industry. The horse gram is an underutilized crop having desired nutrient and bioactive compounds essential for growth and normal body functioning. In view of the above facts, the present study aimed to develop a milk-based smoothie by incorporating horse gram extract in addition to commonly used ingredients such as sugar, dahi, stabilizer, fruits, and vegetables. In this study, three levels of horse gram extract (HGE- 5, 10, and 15%) were blended with cow milk, and other ingredients (sugar, dahi, pectin, banana, carrot juice extract) were kept constant. The 0% level of HGE was taken as a control to compare the nutritional and functional characteristics of the optimized product. The 10% HGE incorporated product was found best based on sensory and physico-chemical properties. The optimized product was found with 3.03±0.08% fat, 3.38±0.07% protein, 16.17±0.10% carbohydrate, 0.81±0.05% ash, 843.33±3.08 cp viscosity, 3.48±0.05 g/10 g whey syneresis, 12.12±0.67 g/20 g sedimentation, and 31.11 ± 4.17% 2,2-dipenylpicrylhydrazyl inhibition activity. The optimized smoothie had nine days shelf life after packing in polypropylene cups when stored under refrigerated conditions.
在过去的几十年里,人们对水果和蔬菜以及果汁和冰沙等混合饮料的需求迅速增长,使其成为食品行业增长最快的细分市场之一。马齿苋是一种未得到充分利用的作物,具有生长和正常身体机能所需的营养和生物活性化合物。鉴于上述事实,本研究旨在开发一种以牛奶为基础的冰沙,除了糖、Dahi、稳定剂、水果和蔬菜等常用配料外,还加入了马齿苋提取物。在这项研究中,牛奶中混合了三种水平的马革提取物(HGE- 5、10 和 15%),其他配料(糖、Dahi、果胶、香蕉、胡萝卜汁提取物)保持不变。以 HGE 含量为 0% 的产品为对照,比较优化产品的营养和功能特性。从感官和理化特性来看,添加 10% HGE 的产品效果最佳。优化产品的脂肪含量为 3.03±0.08%,蛋白质含量为 3.38±0.07%,碳水化合物含量为 16.17±0.10%,灰分含量为 0.81±0.05%,粘度为 843.33±3.08cp,乳清粘滞度为 3.48±0.05g/10g,沉淀度为 12.12±0.67g/20g,2,2-二苯基吡啶肼抑制活性为 31.11±4.17%。优化后的冰沙在聚丙烯杯中包装后,在冷藏条件下可保存 9 天。
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引用次数: 0
Antibacterial Efficacy of Essential Oils from Four Spices against Salmonella typhimurium: Mathematical Modelling and Application in Enhancing Salad Cream Safety 四种香料精油对鼠伤寒沙门氏菌的抗菌功效:数学模型及在提高沙拉奶油安全性中的应用
Pub Date : 2023-12-31 DOI: 10.12944/crnfsj.11.3.30
Phattaraporn Lertchirakarn, Rattana Muangrat
The study was conducted to investigate the inhibitory effects of certain natural substances (finger root, clove, lemongrass, cardamom, and the combination of lemongrass with cardamom) against Salmonella typhimurium, a type of bacteria known to cause foodborne illnesses. The result showed that finger root, clove, lemongrass, cardamom, and the combination of lemongrass with cardamom exhibited strong inhibitory effects against S. typhimurium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated. MIC values ranged from 0.049 to 0.781 µl/ml, and MBC values ranged from 0.049 to 6.250 µl/ml. Furthermore, the study aimed to develop mathematical models that accurately describe S. typhimurium survival in the presence of these essential oils. By understanding how the S. typhimurium respond to the oils over time, it was found that the mathematical models accurately described bacterial survival, with the modified Gompertz model fitting for finger root essential oil and the Weibull and modified Gompertz models suitable for the other oils. Additionally, the study sought to evaluate the practical viability of incorporating these essential oils into salad cream formulations, primarily aiming to assess their potential in reducing S. typhimurium counts and ensuring compliance with established quality standards. Specifically, the inclusion of finger root, clove, lemongrass, cardamom, and the combination of lemongrass with cardamom in salad cream formulations, maintained at a controlled temperature of 4 °C, yielded positive outcomes, meeting the required quality standards. Importantly, the presence of S. typhimurium was rendered undetectable, and an overall reduction in microbial count was observed when compared to cream formulations lacking these essential oils. This study effectively underscores the potential of the examined essential oils as natural antimicrobial agents suitable for incorporation in food products
该研究旨在探讨某些天然物质(指根、丁香、柠檬草、豆蔻以及柠檬草与豆蔻的组合)对鼠伤寒沙门氏菌的抑制作用,鼠伤寒沙门氏菌是一种已知可导致食源性疾病的细菌。结果表明,指根、丁香、柠檬草、小豆蔻以及柠檬草与小豆蔻的组合对鼠伤寒沙门氏菌有很强的抑制作用。对最低抑菌浓度(MIC)和最低杀菌浓度(MBC)进行了评估。MIC 值范围为 0.049 至 0.781 µl/ml,MBC 值范围为 0.049 至 6.250 µl/ml。此外,该研究还旨在建立数学模型,以准确描述伤寒杆菌在这些精油中的存活情况。通过了解鼠伤寒杆菌随着时间的推移对精油的反应,研究发现数学模型能准确描述细菌的存活率,改良贡珀兹模型适合指根精油,Weibull 和改良贡珀兹模型适合其他精油。此外,该研究还试图评估在沙拉奶油配方中添加这些精油的实际可行性,主要目的是评估它们在减少鼠伤寒杆菌数量和确保符合既定质量标准方面的潜力。具体来说,在温度控制在 4 °C 的沙拉酱配方中加入指根、丁香、柠檬草、小豆蔻以及柠檬草与小豆蔻的组合,都能产生积极的效果,达到所要求的质量标准。重要的是,与缺乏这些精油的奶油配方相比,伤寒杆菌的存在变得不可检测,微生物数量总体上也有所减少。这项研究有效地强调了所研究的精油作为天然抗菌剂的潜力,适合添加到食品中。
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Current Research in Nutrition and Food Science Journal
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