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Viability of seeds of some tropical tree species during storage 某些热带树种种子贮藏期间的生存力
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p3279
J. C. F. M. Junior, L. D. F. D. A. Melo, Larice Bruna Ferreira Soares, V. M. Ferreira, J. C. A. Neto, Maria Inajal Rodrigues da Silva das Neves, R. D. A. Paes, E. P. Gonçalves, P. C. Souto, J. F. O. Costa, L. Chaves, Wesley Oliveira de Assis
For most forest species the irregularity of fruiting, low seed production and spatial distribution of trees, make it impossible to supply seeds to meet seedling production programs. On the other hand, to prevent subsequent years of low production and maintain seed viability, it is necessary to adopt adequate storage procedures. Therefore, the objectives of the study were to evaluate the influence of water content and storage conditions on the physiological potential of seeds of Colubrina glandulosa Perkins (Rhamnaceae), Chloroleucon dumosum (Benth.) G.P. Lewis (Fabaceae), Enterolobium contortisiliquum (Vell.) Morong (Fabaceae), Mimosa bimucronata (DC.) O. Kuntze (Fabaceae) and Sapindus saponaria L. (Sapindaceae), which are native tree species in tropical forests in South America. The seeds were placed in paper packaging and glass packaging and stored in a laboratory environment (uncontrolled), during storage periods of zero (recently harvested), three, six, nine, 12 and 15 months. For each storage period, the water content and physiological potential of the seeds were evaluated. The design was entirely randomized with four repetitions of 25 seeds. The germination (G) and germination uniformity (U) data were subjected to analysis of variance in a 2 x 6 factorial arrangement (two types of packaging x six storage periods), and application of polynomial regression. Seeds of Colubrina glandulosa, Chloroleucon dumosum, Enterolobium contortisiliquum, Mimosa bimucronata and Sapindus saponaria, packed in paper packaging or glass packaging, and stored under uncontrolled conditions of natural environment, presented higher G and U, in the storage period of 15 months. The physiological potential of the seeds can be maintained for a long time, as long as they are packed in a glass container with an adequate initial water content. With a water content of 8.2, 8.1, 7.9, 8.4% and 12.1%, the seeds of Colubrina glandulosa, Chloroleucon dumosum, Enterolobium contortisiliquum, Mimosa bimucronata and Sapindus saponaria, respectively, maintain the viability during storage. Therefore, the solution to store these seeds would be to rigorously dry them, and then use waterproof packaging. It is recommended that for the storage of seeds of these tree species, they are previously packaged in glass containers
对于大多数森林树种来说,结果不规律,种子产量低,树木的空间分布,使种子无法满足幼苗生产计划。另一方面,为了防止随后几年的低产量和保持种子活力,有必要采取适当的储存程序。因此,本研究的目的是评价水分含量和贮藏条件对鼠李科(Colubrina glandulsa Perkins)、绿绿松子(Chloroleucon dumosum)种子生理电位的影响。G.P. Lewis(豆科),concortisiliquum (well .)蚕豆科,含羞草(DC.)南美洲热带森林的原生树种:豆科(O. Kuntze)和Sapindus saponaria L. (Sapindaceae)。将种子分别置于纸包装和玻璃包装中,在实验室环境(非受控环境)中分别储存0个月(最近收获的种子)、3个月、6个月、9个月、12个月和15个月。在每个贮藏期,对种子的含水量和生理电位进行测定。该设计完全随机化,每25粒种子重复4次。发芽(G)和发芽均匀性(U)数据采用2 × 6因子(两种包装类型× 6个贮存期)分析方差,并应用多项式回归。在自然环境不受控制的条件下,采用纸包装或玻璃包装,在15个月的储存期中,毛毡花种子、绿绿豆种子、弯曲肠虫种子、含羞草种子和皂荚种子表现出较高的G和U。只要将种子装在初始含水量足够的玻璃容器中,种子的生理潜能就可以保持很长时间。在水分含量分别为8.2、8.1、7.9、8.4%和12.1%的条件下,绿毛茛、绿毛茛、细纹肠、含羞草和皂料种子在贮藏过程中保持活力。因此,储存这些种子的解决方案是将它们严格干燥,然后使用防水包装。建议储存这些树种的种子时,事先将它们包装在玻璃容器中
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引用次数: 0
Interaction of irrigation rates and fertilization doses on postharvest quality of papaya ‘Formosa ’ 灌水量与施肥量对福尔摩沙木瓜采后品质的相互作用
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p3165
L. B. Marinho, Fernanda Nery Vargens, A. F. Neto, Vanessa Rodrigues da Silva, Aureliano Roberto Rodrigues Ramos, L. Vellame, E. Aires
Water deficit is a water-saving strategy that might nevertheless negatively affect fruit quality. In the present study, the papaya quality was evaluated after application of different irrigation rates and nitrogen fertilization doses at harvest and postharvest. The study was conducted on ‘Formosa’ cultivar in semiarid region. The experiment was performed in a completely randomized design in a 2 x 5 x 3 scheme; two storage times (0 and 5 days), five irrigation rates (50, 75, 100, 125, and 150% of crop evapotranspiration), and three nitrogen doses (150, 180, and 210 Kg.ha-1), with four replicates. Total Soluble Solids (TSS), Titratable Acidity (TA), and fruit skin colour (Luminosity - L, intensity of green/red coloration - a*, and intensity of yellow coloration - b*) were evaluated. Reduced irrigation rates and lower nitrogen doses resulted in increased soluble solids and SS/TA ratio. The latter was increased during storage. Irrigation deficit and a lower amount of nitrogen (150 Kg. ha-1) did not reduce papaya quality, even after 5 days of storage in an environment with controlled temperature and humidity. In order to ensure net productivity in rural properties, this water-saving strategy of maintaining the crop under water restriction conditions must take into consideration not only in the curves of papaya quality-related variables, but also fruit productivity curve
水分亏缺是一种节水策略,但可能对果实品质产生负面影响。本研究对采前和采后不同灌溉量和施氮量对木瓜品质的影响进行了评价。以半干旱区的“福尔摩沙”品种为研究对象。试验采用2 × 5 × 3的完全随机设计;2个贮存时间(0和5 d), 5个灌溉量(作物蒸散量的50、75、100、125和150%),3个施氮量(150、180和210 Kg.ha-1), 4个重复。评价了总可溶性固形物(TSS)、可滴定酸度(TA)和果皮颜色(光度- L,绿/红着色强度- a*,黄着色强度- b*)。减少灌溉量和降低施氮量导致可溶性固形物和SS/TA比值升高。后者在贮藏过程中增加。灌溉亏缺和低施氮量(150 Kg)。在控制温度和湿度的环境中储存5天后,Ha-1也没有降低木瓜的品质。为了保证农村财产的净生产力,这种限水条件下保持作物的节水策略不仅要考虑木瓜品质相关变量曲线,而且要考虑果实生产力曲线
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引用次数: 0
Effect of phytoregulators on vegetative characteristics of ‘packham’s triumph’ pear trees 植物调节剂对‘帕克汉凯旋’梨树营养特性的影响
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p2782
A. Lima, M. Giovanaz, Priscila Alvariza Amaral, R. Navroski, J. Fachinello, M. Malgarim
This study aimed at evaluating the use of phytoregulators in vegetative characteristics of ‘Packham’s Triumph’ pear trees in both 2014/2015 and 2015/2016 cycles. The orchard, was implemented in 2011. The following phytoregulators and doses were used: Ethephon and 6-Benzyladenine at 100, 200, 300 and 400 ml L-1. The experiment had a randomized block design with five replicates. Each experimental unit was composed of a plant. Variables under evaluation were leaf area, annual branch length, annual branch diameter, number of buds on the annual branch, internode length, fertility index and trunk cross section areas of the crown cultivar and of the rootstock. Only the following variables exhibited significance: leaf area, annual branch length and number of buds in plants treated with Ethephon in the 2015/2016 cycle. Leaf area was smaller when high concentrations of Ethephon were applied while the least efficient doses for annual branch length and number of buds were 162.66 ml L-1 and 107.30 ml L-1, respectively
本研究旨在评估植物调节剂在2014/2015和2015/2016两个周期对“帕克汉姆凯旋”梨树营养特性的影响。该果园于2011年建成。使用以下植物调节剂和剂量:乙烯利和6-苄基腺嘌呤分别为100、200、300和400 ml L-1。试验采用随机区组设计,共5个重复。每个实验单元由一株植物组成。评价变量为冠型品种和砧木的叶面积、年枝长、年枝径、年枝芽数、节间长、育性指数和树干截面面积。在2015/2016周期内,乙烯利处理植株的叶面积、年分枝长和芽数具有显著性。施用高浓度乙烯利时,叶面积较小,年枝长和芽数的最低有效剂量分别为162.66 ml L-1和107.30 ml L-1
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引用次数: 0
Yield and physiological quality of common bean grains as a function of boron application in the soil 土壤施硼对普通豆粒产量和生理品质的影响
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p3136
Amanda Magalhães Bueno, Aline Fanciel de Andrade, Aline F. S. Carvalho, R. Flores, K. Abdala, G. Santos, M. Mesquita
We carried out a field experiment to evaluate the effects of soil application of sources and doses of boron (B) on production yield and quality of grains of Phaseolus vulgaris (BRS Estilo) grown in an irrigated system. The factorial design was 4x5, being four sources of boron (boric acid, borax, borogran, and FTE BR12) and five doses (0, 1, 2, 3, and 4 kg ha-1) (three replications). We evaluated relative chlorophyll index (RCI), leaf B content, yield, seed viability, and economic value. The data were subjected to analysis of variance, and when significant, evaluated by Tukey test (qualitative) and regression (quantitative). Boron sources affected the RCI 28 days after emergence (DAE). The results for boric acid, borogran, and FTE BR12 were significantly higher (3%, p-value<0.05) than the results for borax. At 46 DAE, boric acid resulted in a RCI 5% higher than the other sources. Borax and boric acid promoted high leaf B contents (126.11 and 105.63 mg dm-3, respectively). The maximum yield (2,224.03 kg ha-1) was obtained by using 2.21 kg ha-1 of B, regardless of the source. Seed viability showed a linear behavior by using borax and boric acid and a quadratic behavior by using borogran and FTE BR12. The dose that resulted in the highest differential profit return (US$ 398.00) was 3.00 kg ha-1. Based on the productive, physiological, and economic results, the dose of 3.00 kg ha-1 provides the highest profit margin for common bean producers
通过田间试验,研究了不同硼源和硼剂量对灌溉条件下菜豆(Phaseolus vulgaris)产量和品质的影响。析因设计为4x5,有四种硼源(硼酸、硼砂、硼格兰和FTE BR12)和五种剂量(0、1、2、3和4 kg ha-1)(3个重复)。我们评估了相对叶绿素指数(RCI)、叶片B含量、产量、种子活力和经济价值。数据进行方差分析,当数据显著时,采用Tukey检验(定性)和回归(定量)进行评估。硼源对羽化后28 d RCI有影响。硼酸、硼砂和FTE BR12的结果显著高于硼砂(3%,p值<0.05)。在46 DAE时,硼酸导致的RCI比其他来源高5%。硼砂和硼酸提高了叶片B含量(分别为126.11和105.63 mg dm-3)。无论来源如何,施用2.21 kg ha-1的B产量最高,为2,224.03 kg ha-1。硼砂和硼酸处理的种子活力呈线性变化,硼砂和FTE BR12处理的种子活力呈二次变化。产生最高差额利润回报(398.00美元)的剂量为3.00公斤每公顷。根据生产、生理和经济结果,3.00 kg hm -1的剂量为普通豆生产者提供了最高的利润率
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引用次数: 0
Maize-Crotalaria spectabilis intercropping in organic system and relations with the insect community 玉米-黄豆间作的有机系统及其与昆虫群落的关系
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p3196
Ivonete Theodoro Araújo, G. G. Zacarin, Erivaldo Silva de Oliveira, Leila Bonfanti, N. D. F. Guimarães, A. D. S. Gallo, A. Fontanetti
The objective of this study was to evaluate the canopy insect community associated with maize intercropped with different arrangements of Crotalaria spectabilis and the effects on the damage caused to maize by Spodoptera frugiperda and Helicoverpa zea. The treatments were intercropping systems of maize with Crotalaria spectabilis: CR - Crotalaria spectabilis sown on the same rows as maize; CBR - Crotalaria spectabilis sown between the rows of maize; CRBR - Crotalaria spectabilis sown on the rows and between the rows of maize, and M - maize monocrop. The experimental plot consisted of five rows of maize, five meters long, spaced 0.8 m apart. Assessments were made of the following parameters: insect community present in the maize canopy, leaf damage caused by the fall armyworm (S. frugiperda) and the corn earworm (H. zea), maize grain yield and shoot dry weight of maize and crotalaria. The CRBR intercropping system was characterized by the presence of predators and parasitoids, especially from the families Forficulidae and Braconidae: 79% and 82%, respectively. The maize monocrop, in turn, was mainly characterized by the presence of chewing and sucking phytophagous insects and predators. There was no influence of plant arrangements on the damage to maize caused by S. frugiperda (mean variation between 0.47 and 0.64 of damage) and H. zea (ranging between 6.42 and 7.49 of damage), neither on the grain yield of the crop (variation between 4129.57 kg ha-1 and 5653.77 kg ha-1). Our results suggest that C. spectablis sown in the rows and between the rows of maize has the potential to optimize conservative biological control, without, however, affecting the grain yield of the cereal
摘要本研究旨在评价玉米间作不同黄穗状夜蛾布置方式对玉米冠层昆虫群落的影响,以及不同布置方式对果夜蛾和玉米穗状夜蛾对玉米危害的影响。两种处理分别是玉米与绿爪草间作:CR -绿爪草与玉米同行播种;玉米行间播种的Crotalaria spectabilis;CRBR - Crotalaria spectabilis播种在玉米的行和行之间,和M -玉米单一作物。试验田由5行玉米组成,每行长5米,每行间隔0.8米。评价指标包括:玉米冠层昆虫群落、秋粘虫和玉米耳虫对叶片的危害、玉米籽粒产量、玉米和玉米花茎干重。CRBR间作系统的主要特征是捕食者和寄生蜂的存在,其中狐螨科和芸螨科的存在率分别为79%和82%。玉米单作的主要特征是存在咀嚼和吸吮的植食性昆虫和捕食者。不同种植方式对玉米的危害程度(平均变异值在0.47 ~ 0.64之间)和玉米蚜(平均变异值在6.42 ~ 7.49之间)没有影响,对玉米产量(变异值在4129.57 ~ 5653.77 kg ha-1之间)也没有影响。我们的研究结果表明,在玉米行间和行间播种,具有优化保守生物防治的潜力,但不会影响谷物的产量
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引用次数: 1
Flow rate changes of drippers with dilutions of treated water produced by oil exploration in the Brazilian semiarid region 巴西半干旱区石油开采处理水稀释后滴管流量的变化
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p2754
H. S. M. Vale, D. O. Costa, R. O. Batista, L. C. A. L. Filho, M. K. T. D. Oliveira, F. A. Oliveira, Marcelo Tavares l Gurge, J. O. Pereira, J.C.S. Portela, Marineide Jussara Diniz, B. Marques, Francisca Evânia de Carvalho, Ana Luiza Veras de Souza
The liquid residue called “produced water” from the exploitation of oil in the ground and sea is generated in large volumes and has significant polluting potential. In the Brazilian semiarid region, this liquid can be applied to the agricultural lands, if properly treated and applied to the soil by dripping. It is an alternative that can mitigate water scarcity and impacts on the environment. However, the vulnerability of drippers to clogging is a problem and can be mitigated with the dilution technique. The flow rate changes of drippers for the application of dilutions of produced water treated (PW) with underground water (UW) was analyzed. The experiment was conducted in a completely randomized split-split-plot design with three replications. Plots consisted of treatments (D1: 100% of UW, D2: 90% of UW and 10% PW, D3: 80% of UW and 20% of PW, D4: 70% of UW and 30% of PW and D5: 60% of UW and 40% of PW). The split-plots consisted of types of drippers (G1: 1.6 L h-1, G2: - 1.6 L h-1, G3: 1.7 L h-1) and split-split-plots consisted of evaluation times (0, 40, 80, 120 and 160 h). Flow rate (D) and flow rate coefficient of variation (FCV) were taken every 40 hours untill 160 h. The results showed that the G3 emitter was the most resistant to clogging. The dilutions D2 and D3 provided the lowest losses in hydraulic performance in the drip units. The highest rates of clogging occurred in the G2 emitter operating in the D5 dilution
地下和海上石油开采产生的被称为“采出水”的液体残留物大量产生,具有严重的污染潜力。在巴西半干旱地区,这种液体可以应用于农业用地,如果适当处理和应用于土壤滴。这是一种可以缓解水资源短缺和对环境影响的替代方案。然而,滴管堵塞的脆弱性是一个问题,可以通过稀释技术加以缓解。分析了地下水处理采出水稀释后滴管的流量变化规律。试验采用完全随机分割-分割图设计,3个重复。各组处理分别为D1: 100% UW, D2: 90% UW和10% PW, D3: 80% UW和20% PW, D4: 70% UW和30% PW, D5: 60% UW和40% PW。分块图由不同滴管类型组成(G1: 1.6 L h-1, G2: - 1.6 L h-1, G3: 1.7 L h-1),分块图由评价时间组成(0、40、80、120和160 h),每隔40 h测定一次流量(D)和流量变异系数(FCV),直至160 h。结果表明,G3滴管最耐堵塞。D2和D3的稀释度对滴注单元的水力性能损失最小。在D5稀释条件下,G2发射器的堵塞率最高
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引用次数: 0
Nutritional and biomass aspects of Helianthus annuus according to boron application in the soil 土壤施硼对向日葵营养和生物量的影响
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p3135
Aline Franciel de Andrade, K. Viçosi, Amanda Magalhães Bueno, R. Flores, Carlos L. R. Santos, G. Santos, M. Mesquita
Sunflower is considered highly demanding in boron (B); however, it has low efficiency in using this micronutrient. An experiment under protected cultivation was carried out to evaluate the effects of boron fertilization on nutrition and sunflower biomass production (Helianthus annuus). A completely randomized design with four replications was used. Five boron doses: 0 (Control), 1, 2, 3, and 4 kg ha-1, were applied to the soil using boric acid as the source. Biometric assessments, measurements of the relative chlorophyll index (RCI), production of dry biomass and the boron use efficiency by plants were carried out. The data were subjected to analysis of variance and F test (p<0.05), with polynomial regression analysis when significant. Leaf area, RCI evaluated at 15 and 60 days, and biomass increase linearly according to the boron application up to the dose of 4 kg ha-1, which corresponds to an increase of 31, 12, 14, and 61%, respectively, compared to control treatment. However, for plant height and nutritional efficiency, the best results were obtained with the dose of 2.64 kg ha-1 of B, with a decrease in higher doses. Boron fertilization in sunflower crop positively influences the nutritional and growth aspects of plants. In general, the application of up to 3 kg ha-1 of B in soils with low natural content increases the crop yield, without phytotoxicity aspects caused by the nutrient
向日葵被认为对硼的需求量很大(B);然而,这种微量营养素的利用效率很低。在保护地栽培条件下,研究了施硼对向日葵营养和生物量产量的影响。采用完全随机设计,共4个重复。以硼酸为源,对土壤施用5种硼剂量:0(对照)、1、2、3和4 kg hm -1。进行了生物计量学评价、相对叶绿素指数(RCI)测定、干生物量产量测定和植物硼利用效率测定。资料进行方差分析和F检验(p<0.05),显著者采用多项式回归分析。15和60 d时叶片面积、RCI和生物量随硼用量的增加呈线性增加,硼用量达到4 kg hm -1,分别比对照增加了31%、12%、14%和61%。但在株高和营养效率方面,以2.64 kg ha-1施硼效果最好,剂量越高越低。施硼对向日葵植株的营养和生长有积极影响。一般来说,在自然含量低的土壤中施用高达3公斤每公顷的B可以增加作物产量,而不会引起营养物的植物毒性
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引用次数: 1
Lines derived from coffee BA-10 with resistance to Pseudomonas syringae under field conditions with simultaneous natural infections of the pathovars garcae and tabaci 从咖啡BA-10中获得的对丁香假单胞菌具有抗性的品系,在野外条件下同时感染病原菌garcae和tabaci
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p2786
C. T. M. Pereira, Fernando Cesar Carducci, L. M. R. Rodrigues, L. Beriam, L. Shigueoka, Valdir Mariucci Junior, Kawana Silva Bortolato, G. H. Sera
Bacterial-halo-blight and bacterial-leaf-spot are important coffee diseases caused by Pseudomonas syringae pv. garcae and pv. tabaci, respectively. The most suitable method to control these diseases is the use of resistant cultivars. There are no studies on resistance to Pseudomonas syringae (PS) in coffee derived from BA-10 genotypes. Therefore, the aim of this study was to evaluate the resistance to PS in lines derived from BA-10 under field conditions with simultaneous natural infections of the pathovars garcae and tabaci. 38 F4 and two F5 lines derived from BA-10 were evaluated in a field trial in Londrina, PR, Brazil. The Catuaí Vermelho IAC 81 and IAPAR 59 were the susceptible and intermediate resistant controls, respectively. Resistance to PS was evaluated in January 2017 after 45 months of planting. The grading scale varied from 1 to 5, where grade 1 was plants with more resistance and 5 plants more susceptible. Two F5 lines showed 100% of resistant plants (grades 1 and 2) and the F4 line IAPAR 12201 showed 60% of plants with a high level of resistance to PS (grade 1), while the control Catuaí showed no resistant plant. IAPAR 59 and several F4 lines showed high frequency of plants with intermediate resistance to PS
细菌性晕叶枯病和细菌性叶斑病是由丁香假单胞菌引起的重要咖啡病害。Garcae和pv。分别地烟。防治这些病害最合适的方法是利用抗病品种。BA-10基因型咖啡对丁香假单胞菌(PS)的抗性研究尚未见报道。因此,本研究的目的是在野外条件下,评价BA-10衍生系对PS的抗性,同时自然感染病原菌garcae和tabaci。从BA-10衍生的38个F4系和2个F5系在巴西PR的Londrina进行了田间试验。Catuaí Vermelho IAC 81和IAPAR 59分别为敏感对照和中抗性对照。种植45个月后,于2017年1月对其抗性进行了评估。等级分为1 ~ 5级,其中1级为抗性较强的植株,5级为易感植株。2个F5系100%抗性(1级和2级),F4系IAPAR 12201 60%抗性高(1级),而对照Catuaí无抗性植株。IAPAR 59和几个F4系对PS具有中等抗性的植株频率较高
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引用次数: 0
Genetic variability of cultivated plum (Prunus domestica L. & Prunus salicina Lindl.) in Morocco assessed by ISSR markers 利用ISSR标记分析了摩洛哥栽培李(Prunus domestica L.和Prunus salicina Lindl.)的遗传变异
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p3217
Youssef Ait Bella, S. Bouda, Y. Khachtib, A. Haddioui
Moroccan plum cultivars were genetically characterized for assessing diversity and relatedness. In this study, a total of 23 plum cultivars were analyzed using 14 Inter-Simple Sequence Repeat (ISSR) primers. A total of 100 bands were obtained, of which 84 were polymorphic (84%). The mean values of PIC, Rp, I and Ht were 0.45; 3.03; 0.42 and 0.27, respectively, implying the important genetic variability between the plum cultivars. Furthermore, the coefficient of genetic differentiation (GST) between all groups was 0.21 indicating that 21 % of total genetic variability was between groups and 79 % was within groups. The UPGMA dendrogram and Bayesian model-based clustering approach identified four gene pools of cultivars independently of their geographic origin and denomination. These results showed that the ISSRs markers can be a useful tool for detecting molecular polymorphism and to survey the genetic diversity in this fruit crop.
对摩洛哥梅品种进行了遗传鉴定,以评估其多样性和亲缘性。本研究利用14条ISSR引物对23个李子品种进行了分析。共获得100条条带,其中84条为多态性,占84%。PIC、Rp、I、Ht的平均值为0.45;3.03;分别为0.42和0.27,表明品种间具有重要的遗传变异。各组间遗传分化系数(GST)为0.21,表明组间遗传变异占总遗传变异的21%,组内遗传变异占总遗传变异的79%。UPGMA树状图和基于贝叶斯模型的聚类方法鉴定了4个独立于地理起源和名称的品种基因库。这些结果表明,ISSRs标记可以作为检测该水果作物分子多态性和调查其遗传多样性的有效工具。
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引用次数: 5
Genetic diversity in sorghum (Sorghum bicolor L. Moench) accessions using SNP based Kompetitive allele-specific (KASP) markers 利用基于SNP的竞争等位基因特异性(KASP)标记分析高粱(sorghum bicolor L. Moench)遗传多样性
Pub Date : 2021-06-10 DOI: 10.21475/ajcs.21.15.06.p3088
Thulo Sejake, N. Shargie, R. Christian, A. Amelework, T. Tsilo
Genetic diversity analysis is an important component in conventional and marker-assisted breeding. The objective of this study was to assess the level of genetic diversity among 100 sorghum accessions, which were selected randomly from the Sorghum National Germplasm Bank maintained at Agricultural Research Council, South Africa. A total of 136 Kompetitive Allele-Specific PCR (KASP) markers were used in this study. The KASP markers were previously derived from single-nucleotide polymorphic (SNP) analysis of the world-wide sorghum accessions by other research groups. A total of 110 KASP markers were polymorphic and recorded an average polymorphic information content (PIC) value of 0.3, which indicated high level of discrimination of the markers. The markers had an average gene diversity and observed heterozygosity of 0.3 and 0.10, respectively. Analysis of molecular variance revealed a significantly high variation among accessions (83% and 89%) than within accessions (10% and 11%) based on breeding status and geographic origin, respectively. Genetic distance varied from 0.0 between SA0672 and SA0673, SA1282 and SA0670 to 0.57 between Hakika and SA1442 with an average mean of 0.30. The dendrogram and model-based population analysis identified three and four distinct groups in 95 sorghum accessions, respectively. These results imply the presence of genetic diversity and lack of genetic bottleneck within the National Sorghum Germplasm Bank, which could be highly relevant for sorghum breeding and germplasm maintenance
遗传多样性分析是传统育种和标记辅助育种的重要组成部分。本研究从南非农业研究委员会高粱国家种质资源库中随机抽取100份高粱材料,对其遗传多样性水平进行了评价。本研究共使用了136个竞争性等位基因特异性PCR (KASP)标记。KASP标记先前由其他研究小组从世界各地高粱材料的单核苷酸多态性(SNP)分析中获得。共有110个KASP标记存在多态性,平均多态性信息含量(PIC)为0.3,表明这些标记具有较高的辨识度。标记的平均基因多样性和杂合度分别为0.3和0.10。分子变异分析表明,品种间(83%)和品种间(89%)的差异显著高于品种间(10%)和品种间(11%)的差异。遗传距离从SA0672与SA0673、SA1282与SA0670之间的0.0变化到Hakika与SA1442之间的0.57,平均为0.30。树状图和基于模型的群体分析在95份高粱材料中分别鉴定出3个和4个不同的类群。这些结果表明,国家高粱种质资源库存在遗传多样性,不存在遗传瓶颈,这对高粱的育种和种质资源维护具有重要意义
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引用次数: 2
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Day 1 Mon, June 28, 2021
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